Experiment: Vitafiber 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05835 and HMPREF1058_RS05830 are separated by 21 nucleotides HMPREF1058_RS05830 and HMPREF1058_RS05825 are separated by 56 nucleotides HMPREF1058_RS05825 and HMPREF1058_RS05820 overlap by 14 nucleotides HMPREF1058_RS05820 and HMPREF1058_RS05815 are separated by 119 nucleotides
HMPREF1058_RS05835: HMPREF1058_RS05835 - 50S ribosomal protein L21, at 69,735 to 70,052
_RS05835
HMPREF1058_RS05830: HMPREF1058_RS05830 - 50S ribosomal protein L27, at 70,074 to 70,337
_RS05830
HMPREF1058_RS05825: HMPREF1058_RS05825 - thiamine diphosphokinase, at 70,394 to 71,029
_RS05825
HMPREF1058_RS05820: HMPREF1058_RS05820 - nicotinamide mononucleotide transporter, at 71,016 to 71,588
_RS05820
HMPREF1058_RS05815: HMPREF1058_RS05815 - serine--tRNA ligase, at 71,708 to 72,982
_RS05815
Position (kb)
71
72 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 70.327 kb on + strand at 70.395 kb on + strand at 70.523 kb on + strand, within HMPREF1058_RS05825 at 70.535 kb on - strand, within HMPREF1058_RS05825 at 70.535 kb on - strand, within HMPREF1058_RS05825 at 70.570 kb on - strand, within HMPREF1058_RS05825 at 70.582 kb on + strand, within HMPREF1058_RS05825 at 70.583 kb on - strand, within HMPREF1058_RS05825 at 70.583 kb on - strand, within HMPREF1058_RS05825 at 70.614 kb on + strand, within HMPREF1058_RS05825 at 70.627 kb on + strand, within HMPREF1058_RS05825 at 70.648 kb on + strand, within HMPREF1058_RS05825 at 70.650 kb on + strand, within HMPREF1058_RS05825 at 70.651 kb on - strand, within HMPREF1058_RS05825 at 70.681 kb on + strand, within HMPREF1058_RS05825 at 70.681 kb on + strand, within HMPREF1058_RS05825 at 70.714 kb on + strand, within HMPREF1058_RS05825 at 70.718 kb on - strand, within HMPREF1058_RS05825 at 70.721 kb on - strand, within HMPREF1058_RS05825 at 70.721 kb on - strand, within HMPREF1058_RS05825 at 70.917 kb on + strand, within HMPREF1058_RS05825 at 71.007 kb on - strand at 71.044 kb on - strand at 71.185 kb on + strand, within HMPREF1058_RS05820 at 71.189 kb on + strand, within HMPREF1058_RS05820 at 71.189 kb on + strand, within HMPREF1058_RS05820 at 71.259 kb on - strand, within HMPREF1058_RS05820 at 71.405 kb on - strand, within HMPREF1058_RS05820 at 71.477 kb on + strand, within HMPREF1058_RS05820 at 71.480 kb on - strand, within HMPREF1058_RS05820 at 71.530 kb on + strand, within HMPREF1058_RS05820 at 71.582 kb on - strand at 71.595 kb on + strand at 71.633 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Vitafiber 10 mg/mL (C) remove 70,327 + +0.8 70,395 + +0.9 70,523 + HMPREF1058_RS05825 0.20 +2.2 70,535 - HMPREF1058_RS05825 0.22 +0.2 70,535 - HMPREF1058_RS05825 0.22 +0.5 70,570 - HMPREF1058_RS05825 0.28 +0.7 70,582 + HMPREF1058_RS05825 0.30 -0.2 70,583 - HMPREF1058_RS05825 0.30 +0.1 70,583 - HMPREF1058_RS05825 0.30 +0.5 70,614 + HMPREF1058_RS05825 0.35 +0.6 70,627 + HMPREF1058_RS05825 0.37 -0.2 70,648 + HMPREF1058_RS05825 0.40 -1.0 70,650 + HMPREF1058_RS05825 0.40 -0.9 70,651 - HMPREF1058_RS05825 0.40 +2.1 70,681 + HMPREF1058_RS05825 0.45 +0.3 70,681 + HMPREF1058_RS05825 0.45 +1.5 70,714 + HMPREF1058_RS05825 0.50 -0.2 70,718 - HMPREF1058_RS05825 0.51 -0.3 70,721 - HMPREF1058_RS05825 0.51 +2.3 70,721 - HMPREF1058_RS05825 0.51 -1.1 70,917 + HMPREF1058_RS05825 0.82 -2.6 71,007 - +0.1 71,044 - -1.7 71,185 + HMPREF1058_RS05820 0.29 +0.4 71,189 + HMPREF1058_RS05820 0.30 -0.9 71,189 + HMPREF1058_RS05820 0.30 +0.5 71,259 - HMPREF1058_RS05820 0.42 -0.5 71,405 - HMPREF1058_RS05820 0.68 -0.1 71,477 + HMPREF1058_RS05820 0.80 +2.1 71,480 - HMPREF1058_RS05820 0.81 +1.6 71,530 + HMPREF1058_RS05820 0.90 +1.0 71,582 - +1.0 71,595 + -1.5 71,633 + +0.4
Or see this region's nucleotide sequence