Experiment: Vitafiber 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05345 and HMPREF1058_RS05340 are separated by 19 nucleotides HMPREF1058_RS05340 and HMPREF1058_RS20985 are separated by 286 nucleotides HMPREF1058_RS20985 and HMPREF1058_RS05335 are separated by 294 nucleotides
HMPREF1058_RS05345: HMPREF1058_RS05345 - site-specific integrase, at 184,979 to 186,196
_RS05345
HMPREF1058_RS05340: HMPREF1058_RS05340 - site-specific integrase, at 186,216 to 187,445
_RS05340
HMPREF1058_RS20985: HMPREF1058_RS20985 - tyrosine-type recombinase/integrase, at 187,732 to 187,899
_RS20985
HMPREF1058_RS05335: HMPREF1058_RS05335 - hypothetical protein, at 188,194 to 188,982
_RS05335
Position (kb) 186
187
188 Strain fitness (log2 ratio) -2
-1
0
1
2 at 185.544 kb on + strand, within HMPREF1058_RS05345 at 185.545 kb on - strand, within HMPREF1058_RS05345 at 185.545 kb on - strand, within HMPREF1058_RS05345 at 185.545 kb on - strand, within HMPREF1058_RS05345 at 185.628 kb on + strand, within HMPREF1058_RS05345 at 185.629 kb on - strand, within HMPREF1058_RS05345 at 185.868 kb on - strand, within HMPREF1058_RS05345 at 185.974 kb on - strand, within HMPREF1058_RS05345 at 186.032 kb on + strand, within HMPREF1058_RS05345 at 186.108 kb on - strand at 186.150 kb on - strand at 186.166 kb on + strand at 186.168 kb on + strand at 186.168 kb on + strand at 186.169 kb on - strand at 186.173 kb on + strand at 186.174 kb on - strand at 186.177 kb on - strand at 186.220 kb on - strand at 186.299 kb on - strand at 186.517 kb on + strand, within HMPREF1058_RS05340 at 186.600 kb on + strand, within HMPREF1058_RS05340 at 186.622 kb on + strand, within HMPREF1058_RS05340 at 186.674 kb on - strand, within HMPREF1058_RS05340 at 186.808 kb on + strand, within HMPREF1058_RS05340 at 186.808 kb on + strand, within HMPREF1058_RS05340 at 186.808 kb on + strand, within HMPREF1058_RS05340 at 186.938 kb on - strand, within HMPREF1058_RS05340 at 187.523 kb on + strand at 187.524 kb on - strand at 187.753 kb on + strand, within HMPREF1058_RS20985 at 187.753 kb on + strand, within HMPREF1058_RS20985 at 187.754 kb on - strand, within HMPREF1058_RS20985 at 187.850 kb on + strand, within HMPREF1058_RS20985 at 187.887 kb on - strand at 187.900 kb on + strand at 187.958 kb on + strand at 188.002 kb on + strand at 188.006 kb on + strand at 188.085 kb on - strand at 188.096 kb on - strand at 188.096 kb on - strand at 188.100 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.228 kb on + strand at 188.231 kb on - strand at 188.317 kb on + strand, within HMPREF1058_RS05335 at 188.318 kb on - strand, within HMPREF1058_RS05335 at 188.318 kb on - strand, within HMPREF1058_RS05335 at 188.344 kb on - strand, within HMPREF1058_RS05335 at 188.348 kb on + strand, within HMPREF1058_RS05335 at 188.421 kb on - strand, within HMPREF1058_RS05335 at 188.437 kb on - strand, within HMPREF1058_RS05335
Per-strain Table
Position Strand Gene LocusTag Fraction Vitafiber 10 mg/mL (C) remove 185,544 + HMPREF1058_RS05345 0.46 -2.1 185,545 - HMPREF1058_RS05345 0.46 +0.3 185,545 - HMPREF1058_RS05345 0.46 -0.2 185,545 - HMPREF1058_RS05345 0.46 +0.1 185,628 + HMPREF1058_RS05345 0.53 +0.1 185,629 - HMPREF1058_RS05345 0.53 -0.0 185,868 - HMPREF1058_RS05345 0.73 -0.5 185,974 - HMPREF1058_RS05345 0.82 -0.9 186,032 + HMPREF1058_RS05345 0.86 -0.4 186,108 - +1.3 186,150 - -0.7 186,166 + -0.0 186,168 + -0.2 186,168 + +1.3 186,169 - -0.6 186,173 + +0.3 186,174 - +0.3 186,177 - +1.4 186,220 - -0.1 186,299 - -0.3 186,517 + HMPREF1058_RS05340 0.24 -0.1 186,600 + HMPREF1058_RS05340 0.31 -0.9 186,622 + HMPREF1058_RS05340 0.33 +0.1 186,674 - HMPREF1058_RS05340 0.37 +1.5 186,808 + HMPREF1058_RS05340 0.48 +0.8 186,808 + HMPREF1058_RS05340 0.48 +1.8 186,808 + HMPREF1058_RS05340 0.48 -0.7 186,938 - HMPREF1058_RS05340 0.59 +0.9 187,523 + -1.7 187,524 - +0.5 187,753 + HMPREF1058_RS20985 0.12 -2.3 187,753 + HMPREF1058_RS20985 0.12 +0.7 187,754 - HMPREF1058_RS20985 0.13 -0.1 187,850 + HMPREF1058_RS20985 0.70 -1.8 187,887 - +2.1 187,900 + +0.6 187,958 + -2.4 188,002 + +0.6 188,006 + -0.6 188,085 - +0.0 188,096 - -0.1 188,096 - +0.8 188,100 + +0.1 188,228 + -2.5 188,228 + +1.7 188,228 + +0.4 188,231 - -0.7 188,317 + HMPREF1058_RS05335 0.16 -0.8 188,318 - HMPREF1058_RS05335 0.16 +0.5 188,318 - HMPREF1058_RS05335 0.16 -1.0 188,344 - HMPREF1058_RS05335 0.19 +0.4 188,348 + HMPREF1058_RS05335 0.20 -0.7 188,421 - HMPREF1058_RS05335 0.29 +0.6 188,437 - HMPREF1058_RS05335 0.31 -1.0
Or see this region's nucleotide sequence