Experiment: FSL 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS0119345 and HMPREF1058_RS17540 overlap by 59 nucleotides HMPREF1058_RS17540 and HMPREF1058_RS17535 are separated by 485 nucleotides HMPREF1058_RS17535 and HMPREF1058_RS17530 are separated by 11 nucleotides
HMPREF1058_RS0119345: HMPREF1058_RS0119345 - 6-bladed beta-propeller, at 402,681 to 403,445
_RS0119345
HMPREF1058_RS17540: HMPREF1058_RS17540 - 6-bladed beta-propeller, at 403,387 to 403,839
_RS17540
HMPREF1058_RS17535: HMPREF1058_RS17535 - IS21-like element helper ATPase IstB, at 404,325 to 405,113
_RS17535
HMPREF1058_RS17530: HMPREF1058_RS17530 - IS21 family transposase, at 405,125 to 406,690
_RS17530
Position (kb) 404
405
406 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 403.370 kb on + strand at 403.380 kb on + strand at 403.381 kb on - strand at 403.388 kb on + strand at 403.391 kb on - strand at 403.419 kb on + strand at 403.422 kb on + strand at 403.424 kb on + strand at 403.424 kb on + strand at 403.424 kb on + strand at 403.424 kb on + strand at 403.425 kb on - strand at 403.426 kb on + strand at 403.427 kb on - strand at 403.427 kb on - strand at 403.428 kb on + strand at 403.429 kb on - strand at 403.429 kb on - strand at 403.429 kb on - strand at 403.430 kb on + strand at 403.431 kb on - strand at 403.464 kb on + strand, within HMPREF1058_RS17540 at 403.464 kb on + strand, within HMPREF1058_RS17540 at 403.466 kb on + strand, within HMPREF1058_RS17540 at 403.504 kb on + strand, within HMPREF1058_RS17540 at 403.550 kb on + strand, within HMPREF1058_RS17540 at 403.551 kb on - strand, within HMPREF1058_RS17540 at 403.703 kb on - strand, within HMPREF1058_RS17540 at 403.711 kb on + strand, within HMPREF1058_RS17540 at 403.712 kb on - strand, within HMPREF1058_RS17540 at 403.765 kb on - strand, within HMPREF1058_RS17540 at 403.767 kb on + strand, within HMPREF1058_RS17540 at 403.768 kb on - strand, within HMPREF1058_RS17540 at 403.770 kb on + strand, within HMPREF1058_RS17540 at 403.801 kb on + strand at 403.801 kb on + strand at 403.804 kb on - strand at 403.812 kb on - strand at 403.872 kb on + strand at 403.893 kb on + strand at 403.916 kb on - strand at 403.993 kb on - strand at 403.993 kb on - strand at 403.997 kb on - strand at 404.038 kb on + strand at 404.038 kb on + strand at 404.039 kb on - strand at 404.039 kb on - strand at 404.044 kb on - strand at 404.049 kb on + strand at 404.062 kb on - strand at 404.086 kb on - strand at 404.086 kb on - strand at 404.115 kb on - strand at 404.162 kb on + strand at 404.173 kb on + strand at 404.624 kb on + strand, within HMPREF1058_RS17535 at 404.631 kb on + strand, within HMPREF1058_RS17535 at 405.529 kb on - strand, within HMPREF1058_RS17530
Per-strain Table
Position Strand Gene LocusTag Fraction FSL 10 mg/mL (C) remove 403,370 + +0.2 403,380 + +2.1 403,381 - -0.1 403,388 + +0.4 403,391 - -1.4 403,419 + +0.7 403,422 + +0.3 403,424 + +0.8 403,424 + -2.5 403,424 + -0.2 403,424 + +0.1 403,425 - -1.1 403,426 + -0.4 403,427 - +0.2 403,427 - -0.1 403,428 + +0.9 403,429 - +1.1 403,429 - -0.8 403,429 - -1.2 403,430 + -1.4 403,431 - +3.1 403,464 + HMPREF1058_RS17540 0.17 +0.2 403,464 + HMPREF1058_RS17540 0.17 -1.1 403,466 + HMPREF1058_RS17540 0.17 -0.5 403,504 + HMPREF1058_RS17540 0.26 +1.1 403,550 + HMPREF1058_RS17540 0.36 +0.8 403,551 - HMPREF1058_RS17540 0.36 -0.0 403,703 - HMPREF1058_RS17540 0.70 -2.8 403,711 + HMPREF1058_RS17540 0.72 -1.2 403,712 - HMPREF1058_RS17540 0.72 +0.2 403,765 - HMPREF1058_RS17540 0.83 -0.9 403,767 + HMPREF1058_RS17540 0.84 -0.9 403,768 - HMPREF1058_RS17540 0.84 -0.7 403,770 + HMPREF1058_RS17540 0.85 -0.2 403,801 + +0.1 403,801 + -0.8 403,804 - -1.4 403,812 - +0.3 403,872 + +1.0 403,893 + -0.1 403,916 - -0.0 403,993 - +1.0 403,993 - -0.6 403,997 - -0.1 404,038 + -1.4 404,038 + +0.4 404,039 - -0.1 404,039 - +0.1 404,044 - +0.1 404,049 + -0.6 404,062 - +2.0 404,086 - -0.0 404,086 - +0.2 404,115 - -0.0 404,162 + +1.5 404,173 + -0.5 404,624 + HMPREF1058_RS17535 0.38 -2.1 404,631 + HMPREF1058_RS17535 0.39 -0.2 405,529 - HMPREF1058_RS17530 0.26 -1.0
Or see this region's nucleotide sequence