Experiment: FSL 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS03975 and HMPREF1058_RS03970 overlap by 13 nucleotides HMPREF1058_RS03970 and HMPREF1058_RS03965 are separated by 93 nucleotides
HMPREF1058_RS03975: HMPREF1058_RS03975 - dihydroorotate dehydrogenase, at 147,562 to 148,473
_RS03975
HMPREF1058_RS03970: HMPREF1058_RS03970 - dihydroorotate dehydrogenase electron transfer subunit, at 148,461 to 149,240
_RS03970
HMPREF1058_RS03965: HMPREF1058_RS03965 - helix-turn-helix transcriptional regulator, at 149,334 to 149,786
_RS03965
Position (kb) 148
149
150 Strain fitness (log2 ratio) -2
-1
0
1
2 at 147.524 kb on + strand at 148.192 kb on - strand, within HMPREF1058_RS03975 at 148.218 kb on - strand at 148.477 kb on - strand at 148.606 kb on - strand, within HMPREF1058_RS03970 at 148.606 kb on - strand, within HMPREF1058_RS03970 at 148.624 kb on + strand, within HMPREF1058_RS03970 at 148.629 kb on - strand, within HMPREF1058_RS03970 at 148.666 kb on + strand, within HMPREF1058_RS03970 at 148.667 kb on - strand, within HMPREF1058_RS03970 at 148.688 kb on + strand, within HMPREF1058_RS03970 at 148.753 kb on + strand at 148.753 kb on + strand, within HMPREF1058_RS03970 at 149.093 kb on + strand at 149.279 kb on - strand at 149.299 kb on + strand at 149.320 kb on - strand at 149.797 kb on - strand at 149.919 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction FSL 10 mg/mL (C) remove 147,524 + +1.0 148,192 - HMPREF1058_RS03975 0.69 +1.2 148,218 - -0.4 148,477 - +0.1 148,606 - HMPREF1058_RS03970 0.19 -0.5 148,606 - HMPREF1058_RS03970 0.19 -1.0 148,624 + HMPREF1058_RS03970 0.21 -0.8 148,629 - HMPREF1058_RS03970 0.22 -0.1 148,666 + HMPREF1058_RS03970 0.26 -0.5 148,667 - HMPREF1058_RS03970 0.26 -0.1 148,688 + HMPREF1058_RS03970 0.29 +0.6 148,753 + +1.2 148,753 + HMPREF1058_RS03970 0.37 -0.3 149,093 + +1.6 149,279 - +1.9 149,299 + -0.5 149,320 - +0.2 149,797 - -2.2 149,919 + -1.1
Or see this region's nucleotide sequence