Experiment: FSL 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS02230 and HMPREF1058_RS02225 are separated by 8 nucleotides HMPREF1058_RS02225 and HMPREF1058_RS02220 are separated by 180 nucleotides HMPREF1058_RS02220 and HMPREF1058_RS0120025 are separated by 17 nucleotides HMPREF1058_RS0120025 and HMPREF1058_RS02215 are separated by 122 nucleotides
HMPREF1058_RS02230: HMPREF1058_RS02230 - exodeoxyribonuclease VII large subunit, at 252,673 to 253,980
_RS02230
HMPREF1058_RS02225: HMPREF1058_RS02225 - exodeoxyribonuclease VII small subunit, at 253,989 to 254,192
_RS02225
HMPREF1058_RS02220: HMPREF1058_RS02220 - branched-chain amino acid aminotransferase, at 254,373 to 255,392
_RS02220
HMPREF1058_RS0120025: HMPREF1058_RS0120025 - DUF975 family protein, at 255,410 to 256,035
_RS0120025
HMPREF1058_RS02215: HMPREF1058_RS02215 - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 256,158 to 256,916
_RS02215
Position (kb)
254
255
256 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 253.373 kb on - strand, within HMPREF1058_RS02230 at 253.373 kb on - strand, within HMPREF1058_RS02230 at 253.407 kb on - strand, within HMPREF1058_RS02230 at 253.410 kb on + strand, within HMPREF1058_RS02230 at 253.410 kb on + strand, within HMPREF1058_RS02230 at 253.410 kb on + strand, within HMPREF1058_RS02230 at 253.414 kb on - strand, within HMPREF1058_RS02230 at 253.425 kb on + strand, within HMPREF1058_RS02230 at 253.486 kb on - strand, within HMPREF1058_RS02230 at 253.507 kb on - strand, within HMPREF1058_RS02230 at 253.508 kb on + strand, within HMPREF1058_RS02230 at 253.569 kb on + strand, within HMPREF1058_RS02230 at 253.570 kb on - strand, within HMPREF1058_RS02230 at 253.570 kb on - strand, within HMPREF1058_RS02230 at 253.616 kb on + strand at 253.617 kb on - strand, within HMPREF1058_RS02230 at 253.617 kb on - strand, within HMPREF1058_RS02230 at 253.617 kb on - strand, within HMPREF1058_RS02230 at 253.697 kb on + strand, within HMPREF1058_RS02230 at 253.697 kb on + strand, within HMPREF1058_RS02230 at 253.863 kb on + strand at 253.864 kb on - strand at 253.864 kb on - strand at 253.867 kb on - strand at 253.963 kb on - strand at 253.964 kb on + strand at 253.965 kb on - strand at 253.965 kb on - strand at 254.010 kb on + strand, within HMPREF1058_RS02225 at 254.054 kb on - strand, within HMPREF1058_RS02225 at 254.054 kb on - strand, within HMPREF1058_RS02225 at 254.060 kb on + strand, within HMPREF1058_RS02225 at 254.225 kb on - strand at 254.263 kb on - strand at 254.319 kb on + strand at 254.367 kb on - strand at 254.372 kb on + strand at 254.372 kb on + strand at 254.410 kb on - strand at 254.424 kb on + strand at 254.425 kb on - strand at 254.425 kb on - strand at 254.426 kb on + strand at 254.431 kb on + strand at 254.431 kb on + strand at 254.431 kb on + strand at 254.431 kb on + strand at 254.573 kb on + strand, within HMPREF1058_RS02220 at 254.622 kb on - strand, within HMPREF1058_RS02220 at 254.740 kb on - strand at 254.763 kb on - strand, within HMPREF1058_RS02220 at 254.800 kb on - strand, within HMPREF1058_RS02220 at 254.870 kb on + strand, within HMPREF1058_RS02220 at 254.935 kb on - strand, within HMPREF1058_RS02220 at 254.935 kb on - strand, within HMPREF1058_RS02220 at 254.994 kb on + strand, within HMPREF1058_RS02220 at 255.041 kb on + strand, within HMPREF1058_RS02220 at 255.069 kb on + strand, within HMPREF1058_RS02220 at 255.105 kb on - strand, within HMPREF1058_RS02220 at 255.105 kb on - strand, within HMPREF1058_RS02220 at 255.278 kb on + strand, within HMPREF1058_RS02220 at 255.285 kb on - strand, within HMPREF1058_RS02220 at 255.339 kb on - strand at 255.345 kb on + strand at 255.362 kb on + strand at 255.362 kb on + strand at 255.369 kb on - strand at 255.386 kb on - strand at 255.393 kb on + strand at 255.402 kb on - strand at 255.484 kb on + strand, within HMPREF1058_RS0120025 at 255.635 kb on + strand, within HMPREF1058_RS0120025 at 255.669 kb on + strand, within HMPREF1058_RS0120025 at 255.693 kb on + strand, within HMPREF1058_RS0120025 at 255.715 kb on + strand, within HMPREF1058_RS0120025 at 255.716 kb on - strand, within HMPREF1058_RS0120025 at 255.716 kb on - strand, within HMPREF1058_RS0120025 at 255.717 kb on + strand, within HMPREF1058_RS0120025 at 255.717 kb on + strand, within HMPREF1058_RS0120025 at 255.766 kb on + strand, within HMPREF1058_RS0120025 at 255.830 kb on + strand, within HMPREF1058_RS0120025 at 255.956 kb on - strand, within HMPREF1058_RS0120025 at 255.956 kb on - strand, within HMPREF1058_RS0120025 at 255.960 kb on - strand, within HMPREF1058_RS0120025 at 255.960 kb on - strand, within HMPREF1058_RS0120025 at 255.960 kb on - strand, within HMPREF1058_RS0120025 at 255.960 kb on - strand, within HMPREF1058_RS0120025 at 255.983 kb on - strand at 256.034 kb on - strand at 256.036 kb on + strand at 256.037 kb on - strand at 256.039 kb on - strand at 256.039 kb on - strand at 256.145 kb on + strand at 256.191 kb on - strand at 256.218 kb on + strand at 256.244 kb on + strand, within HMPREF1058_RS02215 at 256.244 kb on + strand, within HMPREF1058_RS02215 at 256.335 kb on - strand, within HMPREF1058_RS02215 at 256.352 kb on + strand, within HMPREF1058_RS02215 at 256.352 kb on + strand, within HMPREF1058_RS02215 at 256.352 kb on + strand, within HMPREF1058_RS02215 at 256.382 kb on - strand, within HMPREF1058_RS02215 at 256.385 kb on + strand, within HMPREF1058_RS02215
Per-strain Table
Position Strand Gene LocusTag Fraction FSL 10 mg/mL (C) remove 253,373 - HMPREF1058_RS02230 0.54 -1.3 253,373 - HMPREF1058_RS02230 0.54 +0.3 253,407 - HMPREF1058_RS02230 0.56 -0.1 253,410 + HMPREF1058_RS02230 0.56 -0.6 253,410 + HMPREF1058_RS02230 0.56 +0.1 253,410 + HMPREF1058_RS02230 0.56 +0.4 253,414 - HMPREF1058_RS02230 0.57 -0.2 253,425 + HMPREF1058_RS02230 0.57 -0.9 253,486 - HMPREF1058_RS02230 0.62 +0.2 253,507 - HMPREF1058_RS02230 0.64 -0.7 253,508 + HMPREF1058_RS02230 0.64 +3.0 253,569 + HMPREF1058_RS02230 0.69 -0.7 253,570 - HMPREF1058_RS02230 0.69 +0.5 253,570 - HMPREF1058_RS02230 0.69 -0.3 253,616 + +1.8 253,617 - HMPREF1058_RS02230 0.72 -1.4 253,617 - HMPREF1058_RS02230 0.72 +0.3 253,617 - HMPREF1058_RS02230 0.72 -1.3 253,697 + HMPREF1058_RS02230 0.78 +1.7 253,697 + HMPREF1058_RS02230 0.78 +1.4 253,863 + -0.6 253,864 - +0.1 253,864 - +0.8 253,867 - +0.2 253,963 - -0.2 253,964 + +0.8 253,965 - -1.1 253,965 - +1.8 254,010 + HMPREF1058_RS02225 0.10 +0.2 254,054 - HMPREF1058_RS02225 0.32 -0.8 254,054 - HMPREF1058_RS02225 0.32 -0.3 254,060 + HMPREF1058_RS02225 0.35 -0.3 254,225 - -1.6 254,263 - -0.0 254,319 + -0.8 254,367 - -0.6 254,372 + -1.1 254,372 + -3.5 254,410 - -2.1 254,424 + -3.7 254,425 - -1.9 254,425 - -2.7 254,426 + -2.1 254,431 + -3.7 254,431 + -3.8 254,431 + -2.6 254,431 + -3.5 254,573 + HMPREF1058_RS02220 0.20 -3.8 254,622 - HMPREF1058_RS02220 0.24 +0.7 254,740 - -0.6 254,763 - HMPREF1058_RS02220 0.38 -1.6 254,800 - HMPREF1058_RS02220 0.42 -1.5 254,870 + HMPREF1058_RS02220 0.49 -1.5 254,935 - HMPREF1058_RS02220 0.55 -3.8 254,935 - HMPREF1058_RS02220 0.55 -2.7 254,994 + HMPREF1058_RS02220 0.61 -1.4 255,041 + HMPREF1058_RS02220 0.65 -1.0 255,069 + HMPREF1058_RS02220 0.68 -3.1 255,105 - HMPREF1058_RS02220 0.72 -1.3 255,105 - HMPREF1058_RS02220 0.72 -1.5 255,278 + HMPREF1058_RS02220 0.89 -3.3 255,285 - HMPREF1058_RS02220 0.89 -2.0 255,339 - -1.1 255,345 + -1.0 255,362 + -2.1 255,362 + -3.2 255,369 - -2.5 255,386 - -2.2 255,393 + -0.6 255,402 - -0.3 255,484 + HMPREF1058_RS0120025 0.12 +0.2 255,635 + HMPREF1058_RS0120025 0.36 +0.3 255,669 + HMPREF1058_RS0120025 0.41 +0.7 255,693 + HMPREF1058_RS0120025 0.45 -0.3 255,715 + HMPREF1058_RS0120025 0.49 -1.0 255,716 - HMPREF1058_RS0120025 0.49 -0.0 255,716 - HMPREF1058_RS0120025 0.49 +0.2 255,717 + HMPREF1058_RS0120025 0.49 -0.7 255,717 + HMPREF1058_RS0120025 0.49 -0.1 255,766 + HMPREF1058_RS0120025 0.57 +0.6 255,830 + HMPREF1058_RS0120025 0.67 -0.6 255,956 - HMPREF1058_RS0120025 0.87 -1.2 255,956 - HMPREF1058_RS0120025 0.87 -0.0 255,960 - HMPREF1058_RS0120025 0.88 -1.2 255,960 - HMPREF1058_RS0120025 0.88 -1.3 255,960 - HMPREF1058_RS0120025 0.88 +0.4 255,960 - HMPREF1058_RS0120025 0.88 -2.3 255,983 - -0.4 256,034 - +0.9 256,036 + +0.7 256,037 - +1.5 256,039 - -0.3 256,039 - -0.1 256,145 + -0.6 256,191 - +0.8 256,218 + +0.0 256,244 + HMPREF1058_RS02215 0.11 -0.5 256,244 + HMPREF1058_RS02215 0.11 +0.6 256,335 - HMPREF1058_RS02215 0.23 -1.0 256,352 + HMPREF1058_RS02215 0.26 +0.6 256,352 + HMPREF1058_RS02215 0.26 +0.7 256,352 + HMPREF1058_RS02215 0.26 +1.1 256,382 - HMPREF1058_RS02215 0.30 +0.1 256,385 + HMPREF1058_RS02215 0.30 +0.7
Or see this region's nucleotide sequence