Experiment: FSL 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS01460 and HMPREF1058_RS01455 are separated by 2 nucleotides HMPREF1058_RS01455 and HMPREF1058_RS01450 are separated by 24 nucleotides HMPREF1058_RS01450 and HMPREF1058_RS01445 overlap by 35 nucleotides HMPREF1058_RS01445 and HMPREF1058_RS21720 are separated by 709 nucleotides
HMPREF1058_RS01460: HMPREF1058_RS01460 - acyl-CoA carboxylase subunit beta, at 119,792 to 121,324
_RS01460
HMPREF1058_RS01455: HMPREF1058_RS01455 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 121,327 to 121,842
_RS01455
HMPREF1058_RS01450: HMPREF1058_RS01450 - acetyl-CoA carboxylase biotin carboxylase subunit, at 121,867 to 123,378
_RS01450
HMPREF1058_RS01445: HMPREF1058_RS01445 - cysteine-rich CWC family protein, at 123,344 to 123,595
_RS01445
HMPREF1058_RS21720: HMPREF1058_RS21720 - ribonucleoside-triphosphate reductase, at 124,305 to 124,805
_RS21720
Position (kb) 121
122
123
124 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 123.201 kb on - strand, within HMPREF1058_RS01450 at 123.214 kb on + strand, within HMPREF1058_RS01450 at 123.214 kb on + strand, within HMPREF1058_RS01450 at 123.215 kb on - strand, within HMPREF1058_RS01450 at 123.215 kb on - strand, within HMPREF1058_RS01450 at 123.248 kb on + strand at 123.417 kb on + strand, within HMPREF1058_RS01445 at 123.469 kb on - strand, within HMPREF1058_RS01445 at 123.484 kb on - strand, within HMPREF1058_RS01445 at 123.578 kb on + strand at 123.967 kb on + strand at 124.038 kb on - strand at 124.051 kb on + strand at 124.211 kb on + strand at 124.263 kb on - strand at 124.286 kb on - strand at 124.288 kb on - strand at 124.299 kb on - strand at 124.304 kb on + strand at 124.322 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction FSL 10 mg/mL (C) remove 123,201 - HMPREF1058_RS01450 0.88 -1.0 123,214 + HMPREF1058_RS01450 0.89 -2.8 123,214 + HMPREF1058_RS01450 0.89 -3.0 123,215 - HMPREF1058_RS01450 0.89 -1.9 123,215 - HMPREF1058_RS01450 0.89 -2.2 123,248 + -0.6 123,417 + HMPREF1058_RS01445 0.29 -1.6 123,469 - HMPREF1058_RS01445 0.50 -0.2 123,484 - HMPREF1058_RS01445 0.56 -0.4 123,578 + -1.4 123,967 + -0.0 124,038 - -0.6 124,051 + -0.3 124,211 + +0.6 124,263 - +1.4 124,286 - -0.8 124,288 - +0.8 124,299 - -0.6 124,304 + -1.1 124,322 - -1.1
Or see this region's nucleotide sequence