Experiment: FSL 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS01075 and HMPREF1058_RS01065 are separated by 653 nucleotides HMPREF1058_RS01065 and HMPREF1058_RS01060 are separated by 18 nucleotides HMPREF1058_RS01060 and HMPREF1058_RS21725 overlap by 11 nucleotides
HMPREF1058_RS01075: HMPREF1058_RS01075 - site-specific integrase, at 215,799 to 217,013
_RS01075
HMPREF1058_RS01065: HMPREF1058_RS01065 - hypothetical protein, at 217,667 to 218,284
_RS01065
HMPREF1058_RS01060: HMPREF1058_RS01060 - hypothetical protein, at 218,303 to 218,827
_RS01060
HMPREF1058_RS21725: HMPREF1058_RS21725 - hypothetical protein, at 218,817 to 219,350
_RS21725
Position (kb) 217
218
219 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 217.144 kb on + strand at 217.145 kb on - strand at 217.145 kb on - strand at 217.170 kb on - strand at 217.321 kb on - strand at 217.323 kb on + strand at 217.323 kb on + strand at 217.358 kb on - strand at 217.360 kb on - strand at 217.453 kb on - strand at 217.563 kb on + strand at 217.603 kb on - strand at 217.603 kb on - strand at 217.606 kb on - strand at 217.634 kb on - strand at 217.643 kb on + strand at 217.643 kb on + strand at 217.644 kb on - strand at 217.679 kb on - strand at 217.691 kb on - strand at 217.693 kb on + strand at 217.740 kb on - strand, within HMPREF1058_RS01065 at 217.854 kb on + strand, within HMPREF1058_RS01065 at 217.882 kb on - strand, within HMPREF1058_RS01065 at 217.884 kb on - strand, within HMPREF1058_RS01065 at 217.913 kb on - strand, within HMPREF1058_RS01065 at 217.914 kb on + strand, within HMPREF1058_RS01065 at 217.914 kb on + strand, within HMPREF1058_RS01065 at 218.125 kb on + strand, within HMPREF1058_RS01065 at 218.246 kb on + strand at 218.246 kb on + strand at 218.247 kb on - strand at 218.247 kb on - strand at 218.248 kb on + strand at 218.289 kb on - strand at 218.313 kb on + strand at 218.359 kb on - strand, within HMPREF1058_RS01060 at 218.454 kb on + strand, within HMPREF1058_RS01060 at 218.454 kb on + strand, within HMPREF1058_RS01060 at 218.454 kb on + strand, within HMPREF1058_RS01060 at 218.454 kb on + strand, within HMPREF1058_RS01060 at 218.455 kb on - strand, within HMPREF1058_RS01060 at 218.455 kb on - strand, within HMPREF1058_RS01060 at 218.455 kb on - strand, within HMPREF1058_RS01060 at 218.470 kb on - strand, within HMPREF1058_RS01060 at 218.475 kb on + strand, within HMPREF1058_RS01060 at 218.475 kb on + strand, within HMPREF1058_RS01060 at 218.570 kb on - strand, within HMPREF1058_RS01060 at 218.571 kb on + strand, within HMPREF1058_RS01060 at 218.747 kb on - strand, within HMPREF1058_RS01060 at 218.747 kb on - strand, within HMPREF1058_RS01060 at 218.810 kb on - strand at 218.816 kb on + strand at 218.825 kb on + strand at 218.855 kb on - strand at 218.951 kb on + strand, within HMPREF1058_RS21725 at 219.008 kb on + strand, within HMPREF1058_RS21725 at 219.009 kb on - strand, within HMPREF1058_RS21725 at 219.009 kb on - strand, within HMPREF1058_RS21725 at 219.023 kb on + strand, within HMPREF1058_RS21725 at 219.049 kb on - strand, within HMPREF1058_RS21725 at 219.049 kb on - strand, within HMPREF1058_RS21725 at 219.113 kb on - strand, within HMPREF1058_RS21725 at 219.113 kb on - strand, within HMPREF1058_RS21725 at 219.113 kb on - strand, within HMPREF1058_RS21725 at 219.113 kb on - strand, within HMPREF1058_RS21725 at 219.191 kb on + strand, within HMPREF1058_RS21725 at 219.192 kb on - strand, within HMPREF1058_RS21725
Per-strain Table
Position Strand Gene LocusTag Fraction FSL 10 mg/mL (C) remove 217,144 + +0.1 217,145 - -0.2 217,145 - -1.1 217,170 - -1.5 217,321 - +1.6 217,323 + +2.2 217,323 + +3.2 217,358 - -1.9 217,360 - +1.1 217,453 - -2.8 217,563 + +2.2 217,603 - +0.7 217,603 - -0.3 217,606 - +0.5 217,634 - -2.2 217,643 + -0.1 217,643 + -1.4 217,644 - +1.1 217,679 - +0.2 217,691 - -1.1 217,693 + +0.2 217,740 - HMPREF1058_RS01065 0.12 -0.4 217,854 + HMPREF1058_RS01065 0.30 -0.2 217,882 - HMPREF1058_RS01065 0.35 -1.6 217,884 - HMPREF1058_RS01065 0.35 -1.6 217,913 - HMPREF1058_RS01065 0.40 -0.6 217,914 + HMPREF1058_RS01065 0.40 -0.1 217,914 + HMPREF1058_RS01065 0.40 -0.0 218,125 + HMPREF1058_RS01065 0.74 -1.4 218,246 + +0.6 218,246 + +0.6 218,247 - -1.7 218,247 - +0.0 218,248 + +1.4 218,289 - -0.1 218,313 + +0.1 218,359 - HMPREF1058_RS01060 0.11 -1.0 218,454 + HMPREF1058_RS01060 0.29 +0.2 218,454 + HMPREF1058_RS01060 0.29 -0.3 218,454 + HMPREF1058_RS01060 0.29 +0.0 218,454 + HMPREF1058_RS01060 0.29 -0.5 218,455 - HMPREF1058_RS01060 0.29 -1.0 218,455 - HMPREF1058_RS01060 0.29 +1.2 218,455 - HMPREF1058_RS01060 0.29 -0.4 218,470 - HMPREF1058_RS01060 0.32 -1.2 218,475 + HMPREF1058_RS01060 0.33 +0.1 218,475 + HMPREF1058_RS01060 0.33 -0.3 218,570 - HMPREF1058_RS01060 0.51 +0.1 218,571 + HMPREF1058_RS01060 0.51 +0.4 218,747 - HMPREF1058_RS01060 0.85 -0.2 218,747 - HMPREF1058_RS01060 0.85 -1.0 218,810 - -0.2 218,816 + +0.9 218,825 + +0.3 218,855 - -0.8 218,951 + HMPREF1058_RS21725 0.25 -1.2 219,008 + HMPREF1058_RS21725 0.36 -2.9 219,009 - HMPREF1058_RS21725 0.36 +1.4 219,009 - HMPREF1058_RS21725 0.36 +0.2 219,023 + HMPREF1058_RS21725 0.39 -0.2 219,049 - HMPREF1058_RS21725 0.43 +0.5 219,049 - HMPREF1058_RS21725 0.43 +0.5 219,113 - HMPREF1058_RS21725 0.55 -0.2 219,113 - HMPREF1058_RS21725 0.55 -1.0 219,113 - HMPREF1058_RS21725 0.55 -2.0 219,113 - HMPREF1058_RS21725 0.55 -0.5 219,191 + HMPREF1058_RS21725 0.70 -0.7 219,192 - HMPREF1058_RS21725 0.70 +0.6
Or see this region's nucleotide sequence