Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS04520 and HMPREF1058_RS04515 are separated by 3 nucleotides HMPREF1058_RS04515 and HMPREF1058_RS20295 are separated by 121 nucleotides HMPREF1058_RS20295 and HMPREF1058_RS04510 are separated by 20 nucleotides HMPREF1058_RS04510 and HMPREF1058_RS04505 are separated by 7 nucleotides
HMPREF1058_RS04520: HMPREF1058_RS04520 - hypothetical protein, at 12,711 to 12,926
_RS04520
HMPREF1058_RS04515: HMPREF1058_RS04515 - hypothetical protein, at 12,930 to 13,127
_RS04515
HMPREF1058_RS20295: HMPREF1058_RS20295 - hypothetical protein, at 13,249 to 13,641
_RS20295
HMPREF1058_RS04510: HMPREF1058_RS04510 - hypothetical protein, at 13,662 to 14,102
_RS04510
HMPREF1058_RS04505: HMPREF1058_RS04505 - type I restriction enzyme HsdR N-terminal domain-containing protein, at 14,110 to 15,195
_RS04505
Position (kb) 13
14 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 12.331 kb on + strand at 12.553 kb on + strand at 12.573 kb on + strand at 12.573 kb on + strand at 12.676 kb on + strand at 12.712 kb on + strand at 12.814 kb on + strand, within HMPREF1058_RS04520 at 12.931 kb on + strand at 12.931 kb on + strand at 13.009 kb on + strand, within HMPREF1058_RS04515 at 13.020 kb on + strand, within HMPREF1058_RS04515 at 13.130 kb on + strand at 13.455 kb on + strand, within HMPREF1058_RS20295 at 13.455 kb on + strand, within HMPREF1058_RS20295 at 13.465 kb on + strand, within HMPREF1058_RS20295 at 13.466 kb on - strand, within HMPREF1058_RS20295 at 13.646 kb on + strand at 13.651 kb on - strand at 13.674 kb on - strand at 13.674 kb on - strand at 13.746 kb on - strand, within HMPREF1058_RS04510 at 13.746 kb on - strand, within HMPREF1058_RS04510 at 13.877 kb on + strand, within HMPREF1058_RS04510 at 13.879 kb on + strand, within HMPREF1058_RS04510 at 13.879 kb on + strand, within HMPREF1058_RS04510 at 13.880 kb on - strand, within HMPREF1058_RS04510 at 13.949 kb on + strand, within HMPREF1058_RS04510 at 13.950 kb on - strand, within HMPREF1058_RS04510 at 14.006 kb on - strand, within HMPREF1058_RS04510 at 14.043 kb on + strand, within HMPREF1058_RS04510 at 14.044 kb on - strand, within HMPREF1058_RS04510 at 14.045 kb on + strand, within HMPREF1058_RS04510 at 14.053 kb on - strand, within HMPREF1058_RS04510 at 14.105 kb on + strand at 14.128 kb on - strand at 14.129 kb on + strand at 14.237 kb on + strand, within HMPREF1058_RS04505 at 14.238 kb on - strand, within HMPREF1058_RS04505 at 14.238 kb on - strand, within HMPREF1058_RS04505 at 14.256 kb on - strand, within HMPREF1058_RS04505 at 14.342 kb on + strand, within HMPREF1058_RS04505 at 14.342 kb on + strand, within HMPREF1058_RS04505 at 14.398 kb on + strand, within HMPREF1058_RS04505 at 14.399 kb on - strand, within HMPREF1058_RS04505 at 14.401 kb on + strand, within HMPREF1058_RS04505 at 14.402 kb on - strand, within HMPREF1058_RS04505 at 14.541 kb on - strand, within HMPREF1058_RS04505 at 14.627 kb on + strand, within HMPREF1058_RS04505
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 12,331 + +0.8 12,553 + -0.4 12,573 + -0.7 12,573 + +0.9 12,676 + -2.0 12,712 + +1.3 12,814 + HMPREF1058_RS04520 0.48 -2.7 12,931 + +1.9 12,931 + +1.8 13,009 + HMPREF1058_RS04515 0.40 -0.1 13,020 + HMPREF1058_RS04515 0.45 -1.5 13,130 + +0.5 13,455 + HMPREF1058_RS20295 0.52 -0.2 13,455 + HMPREF1058_RS20295 0.52 -2.2 13,465 + HMPREF1058_RS20295 0.55 +3.2 13,466 - HMPREF1058_RS20295 0.55 -2.9 13,646 + -0.2 13,651 - -0.6 13,674 - +0.4 13,674 - +0.5 13,746 - HMPREF1058_RS04510 0.19 -1.1 13,746 - HMPREF1058_RS04510 0.19 +0.5 13,877 + HMPREF1058_RS04510 0.49 +0.7 13,879 + HMPREF1058_RS04510 0.49 -0.4 13,879 + HMPREF1058_RS04510 0.49 -0.8 13,880 - HMPREF1058_RS04510 0.49 -1.6 13,949 + HMPREF1058_RS04510 0.65 -0.8 13,950 - HMPREF1058_RS04510 0.65 +2.2 14,006 - HMPREF1058_RS04510 0.78 -0.7 14,043 + HMPREF1058_RS04510 0.86 +1.9 14,044 - HMPREF1058_RS04510 0.87 +1.1 14,045 + HMPREF1058_RS04510 0.87 +1.7 14,053 - HMPREF1058_RS04510 0.89 +0.3 14,105 + +0.2 14,128 - +0.4 14,129 + +4.2 14,237 + HMPREF1058_RS04505 0.12 -0.1 14,238 - HMPREF1058_RS04505 0.12 +1.2 14,238 - HMPREF1058_RS04505 0.12 -0.7 14,256 - HMPREF1058_RS04505 0.13 -1.2 14,342 + HMPREF1058_RS04505 0.21 -3.2 14,342 + HMPREF1058_RS04505 0.21 +1.7 14,398 + HMPREF1058_RS04505 0.27 +0.5 14,399 - HMPREF1058_RS04505 0.27 +0.7 14,401 + HMPREF1058_RS04505 0.27 -1.3 14,402 - HMPREF1058_RS04505 0.27 +1.1 14,541 - HMPREF1058_RS04505 0.40 -0.4 14,627 + HMPREF1058_RS04505 0.48 +1.1
Or see this region's nucleotide sequence