Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS15890 and HMPREF1058_RS15885 are separated by 89 nucleotides HMPREF1058_RS15885 and HMPREF1058_RS15880 are separated by 3 nucleotides
HMPREF1058_RS15890: HMPREF1058_RS15890 - NAD(+) synthase, at 811,810 to 813,735
_RS15890
HMPREF1058_RS15885: HMPREF1058_RS15885 - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 813,825 to 814,943
_RS15885
HMPREF1058_RS15880: HMPREF1058_RS15880 - histidinol-phosphate transaminase, at 814,947 to 815,987
_RS15880
Position (kb) 813
814
815 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 813.743 kb on - strand at 813.778 kb on - strand at 813.800 kb on - strand at 813.800 kb on - strand at 813.804 kb on + strand at 813.831 kb on - strand at 813.831 kb on - strand at 813.831 kb on - strand at 813.850 kb on - strand at 813.921 kb on + strand at 813.922 kb on - strand at 813.926 kb on + strand at 813.960 kb on + strand, within HMPREF1058_RS15885 at 814.172 kb on + strand, within HMPREF1058_RS15885 at 814.172 kb on + strand, within HMPREF1058_RS15885 at 814.172 kb on + strand at 814.205 kb on + strand, within HMPREF1058_RS15885 at 814.208 kb on + strand, within HMPREF1058_RS15885 at 814.209 kb on - strand, within HMPREF1058_RS15885 at 814.240 kb on - strand, within HMPREF1058_RS15885 at 814.357 kb on + strand, within HMPREF1058_RS15885 at 814.358 kb on - strand, within HMPREF1058_RS15885 at 814.358 kb on - strand, within HMPREF1058_RS15885 at 814.358 kb on - strand, within HMPREF1058_RS15885 at 814.361 kb on + strand, within HMPREF1058_RS15885 at 814.397 kb on + strand, within HMPREF1058_RS15885 at 814.397 kb on + strand, within HMPREF1058_RS15885 at 814.453 kb on - strand, within HMPREF1058_RS15885 at 814.494 kb on - strand, within HMPREF1058_RS15885 at 814.840 kb on - strand at 814.840 kb on - strand at 814.910 kb on + strand at 814.911 kb on - strand at 814.926 kb on - strand at 814.944 kb on - strand at 814.957 kb on + strand at 815.040 kb on - strand at 815.059 kb on - strand, within HMPREF1058_RS15880 at 815.074 kb on - strand, within HMPREF1058_RS15880 at 815.080 kb on + strand, within HMPREF1058_RS15880 at 815.088 kb on + strand, within HMPREF1058_RS15880 at 815.088 kb on + strand, within HMPREF1058_RS15880 at 815.112 kb on - strand, within HMPREF1058_RS15880 at 815.230 kb on + strand, within HMPREF1058_RS15880 at 815.294 kb on + strand, within HMPREF1058_RS15880 at 815.295 kb on - strand, within HMPREF1058_RS15880 at 815.295 kb on - strand, within HMPREF1058_RS15880 at 815.310 kb on - strand, within HMPREF1058_RS15880 at 815.405 kb on - strand, within HMPREF1058_RS15880 at 815.408 kb on + strand, within HMPREF1058_RS15880 at 815.463 kb on + strand, within HMPREF1058_RS15880 at 815.671 kb on + strand, within HMPREF1058_RS15880 at 815.672 kb on - strand, within HMPREF1058_RS15880 at 815.672 kb on - strand, within HMPREF1058_RS15880 at 815.675 kb on + strand, within HMPREF1058_RS15880 at 815.675 kb on + strand, within HMPREF1058_RS15880 at 815.676 kb on - strand, within HMPREF1058_RS15880 at 815.676 kb on - strand, within HMPREF1058_RS15880 at 815.676 kb on - strand, within HMPREF1058_RS15880 at 815.684 kb on + strand, within HMPREF1058_RS15880 at 815.700 kb on + strand, within HMPREF1058_RS15880 at 815.813 kb on + strand, within HMPREF1058_RS15880 at 815.814 kb on - strand, within HMPREF1058_RS15880 at 815.835 kb on - strand, within HMPREF1058_RS15880 at 815.835 kb on - strand, within HMPREF1058_RS15880 at 815.855 kb on + strand, within HMPREF1058_RS15880 at 815.856 kb on - strand, within HMPREF1058_RS15880 at 815.861 kb on + strand, within HMPREF1058_RS15880 at 815.862 kb on - strand, within HMPREF1058_RS15880 at 815.912 kb on + strand at 815.935 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 813,743 - +0.5 813,778 - -0.8 813,800 - +1.8 813,800 - +1.8 813,804 + -0.2 813,831 - -3.8 813,831 - -5.2 813,831 - +2.8 813,850 - -0.7 813,921 + -1.7 813,922 - -1.5 813,926 + +1.8 813,960 + HMPREF1058_RS15885 0.12 -2.4 814,172 + HMPREF1058_RS15885 0.31 -2.6 814,172 + HMPREF1058_RS15885 0.31 +0.6 814,172 + +1.6 814,205 + HMPREF1058_RS15885 0.34 +0.7 814,208 + HMPREF1058_RS15885 0.34 -2.0 814,209 - HMPREF1058_RS15885 0.34 -3.3 814,240 - HMPREF1058_RS15885 0.37 +0.1 814,357 + HMPREF1058_RS15885 0.48 -2.0 814,358 - HMPREF1058_RS15885 0.48 -4.4 814,358 - HMPREF1058_RS15885 0.48 -2.9 814,358 - HMPREF1058_RS15885 0.48 -2.7 814,361 + HMPREF1058_RS15885 0.48 -1.5 814,397 + HMPREF1058_RS15885 0.51 -1.6 814,397 + HMPREF1058_RS15885 0.51 -1.1 814,453 - HMPREF1058_RS15885 0.56 -2.3 814,494 - HMPREF1058_RS15885 0.60 -1.5 814,840 - -4.0 814,840 - +1.8 814,910 + -3.0 814,911 - +0.4 814,926 - -2.2 814,944 - -1.0 814,957 + -1.2 815,040 - -1.3 815,059 - HMPREF1058_RS15880 0.11 -2.0 815,074 - HMPREF1058_RS15880 0.12 -1.6 815,080 + HMPREF1058_RS15880 0.13 -2.1 815,088 + HMPREF1058_RS15880 0.14 -1.6 815,088 + HMPREF1058_RS15880 0.14 -3.5 815,112 - HMPREF1058_RS15880 0.16 -1.8 815,230 + HMPREF1058_RS15880 0.27 -1.1 815,294 + HMPREF1058_RS15880 0.33 +0.7 815,295 - HMPREF1058_RS15880 0.33 -2.2 815,295 - HMPREF1058_RS15880 0.33 -0.9 815,310 - HMPREF1058_RS15880 0.35 -1.6 815,405 - HMPREF1058_RS15880 0.44 -1.5 815,408 + HMPREF1058_RS15880 0.44 -3.4 815,463 + HMPREF1058_RS15880 0.50 -0.4 815,671 + HMPREF1058_RS15880 0.70 -1.1 815,672 - HMPREF1058_RS15880 0.70 -3.0 815,672 - HMPREF1058_RS15880 0.70 -2.0 815,675 + HMPREF1058_RS15880 0.70 -2.6 815,675 + HMPREF1058_RS15880 0.70 -1.1 815,676 - HMPREF1058_RS15880 0.70 -1.4 815,676 - HMPREF1058_RS15880 0.70 +1.6 815,676 - HMPREF1058_RS15880 0.70 -2.3 815,684 + HMPREF1058_RS15880 0.71 -1.2 815,700 + HMPREF1058_RS15880 0.72 -2.5 815,813 + HMPREF1058_RS15880 0.83 -0.1 815,814 - HMPREF1058_RS15880 0.83 +0.5 815,835 - HMPREF1058_RS15880 0.85 +1.6 815,835 - HMPREF1058_RS15880 0.85 -1.9 815,855 + HMPREF1058_RS15880 0.87 +0.2 815,856 - HMPREF1058_RS15880 0.87 -2.6 815,861 + HMPREF1058_RS15880 0.88 -1.7 815,862 - HMPREF1058_RS15880 0.88 -0.5 815,912 + -0.3 815,935 + -0.9
Or see this region's nucleotide sequence