Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS20515 and HMPREF1058_RS14610 are separated by 286 nucleotides HMPREF1058_RS14610 and HMPREF1058_RS14605 are separated by 16 nucleotides HMPREF1058_RS14605 and HMPREF1058_RS14600 are separated by 30 nucleotides
HMPREF1058_RS20515: HMPREF1058_RS20515 - DUF4373 domain-containing protein, at 1,157,710 to 1,158,464
_RS20515
HMPREF1058_RS14610: HMPREF1058_RS14610 - adenylosuccinate synthase, at 1,158,751 to 1,160,022
_RS14610
HMPREF1058_RS14605: HMPREF1058_RS14605 - transcriptional repressor, at 1,160,039 to 1,160,530
_RS14605
HMPREF1058_RS14600: HMPREF1058_RS14600 - DNA alkylation repair protein, at 1,160,561 to 1,161,229
_RS14600
Position (kb)
1158
1159
1160
1161 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4 at 1157.753 kb on - strand at 1157.769 kb on + strand at 1157.769 kb on + strand at 1157.775 kb on + strand at 1157.785 kb on + strand at 1157.786 kb on - strand, within HMPREF1058_RS20515 at 1157.816 kb on + strand, within HMPREF1058_RS20515 at 1157.838 kb on + strand, within HMPREF1058_RS20515 at 1157.838 kb on + strand, within HMPREF1058_RS20515 at 1157.839 kb on - strand, within HMPREF1058_RS20515 at 1157.903 kb on - strand, within HMPREF1058_RS20515 at 1157.903 kb on - strand, within HMPREF1058_RS20515 at 1157.911 kb on - strand, within HMPREF1058_RS20515 at 1158.000 kb on - strand, within HMPREF1058_RS20515 at 1158.163 kb on + strand, within HMPREF1058_RS20515 at 1158.164 kb on - strand, within HMPREF1058_RS20515 at 1158.248 kb on + strand, within HMPREF1058_RS20515 at 1158.340 kb on + strand, within HMPREF1058_RS20515 at 1158.340 kb on + strand, within HMPREF1058_RS20515 at 1158.340 kb on + strand, within HMPREF1058_RS20515 at 1158.359 kb on + strand, within HMPREF1058_RS20515 at 1158.359 kb on + strand, within HMPREF1058_RS20515 at 1158.371 kb on - strand, within HMPREF1058_RS20515 at 1158.396 kb on + strand at 1158.397 kb on - strand at 1158.493 kb on - strand at 1158.531 kb on + strand at 1158.534 kb on + strand at 1158.535 kb on - strand at 1158.541 kb on + strand at 1158.577 kb on - strand at 1158.577 kb on - strand at 1158.593 kb on + strand at 1158.594 kb on - strand at 1158.611 kb on + strand at 1158.611 kb on + strand at 1158.616 kb on + strand at 1158.621 kb on + strand at 1158.622 kb on - strand at 1158.637 kb on + strand at 1158.637 kb on + strand at 1158.638 kb on - strand at 1158.642 kb on - strand at 1158.661 kb on + strand at 1158.666 kb on - strand at 1158.666 kb on - strand at 1158.675 kb on + strand at 1158.675 kb on + strand at 1158.681 kb on + strand at 1158.734 kb on + strand at 1158.752 kb on + strand at 1158.752 kb on + strand at 1158.775 kb on - strand at 1158.848 kb on + strand at 1158.849 kb on - strand at 1158.849 kb on - strand at 1158.927 kb on - strand, within HMPREF1058_RS14610 at 1158.927 kb on - strand, within HMPREF1058_RS14610 at 1158.928 kb on + strand, within HMPREF1058_RS14610 at 1158.951 kb on + strand, within HMPREF1058_RS14610 at 1158.957 kb on - strand, within HMPREF1058_RS14610 at 1158.985 kb on + strand, within HMPREF1058_RS14610 at 1158.986 kb on - strand, within HMPREF1058_RS14610 at 1158.992 kb on + strand, within HMPREF1058_RS14610 at 1158.993 kb on - strand, within HMPREF1058_RS14610 at 1159.048 kb on - strand, within HMPREF1058_RS14610 at 1159.052 kb on - strand, within HMPREF1058_RS14610 at 1159.077 kb on + strand, within HMPREF1058_RS14610 at 1159.077 kb on + strand, within HMPREF1058_RS14610 at 1159.077 kb on + strand, within HMPREF1058_RS14610 at 1159.077 kb on + strand, within HMPREF1058_RS14610 at 1159.078 kb on - strand, within HMPREF1058_RS14610 at 1159.078 kb on - strand, within HMPREF1058_RS14610 at 1159.082 kb on + strand, within HMPREF1058_RS14610 at 1159.082 kb on + strand, within HMPREF1058_RS14610 at 1159.093 kb on + strand, within HMPREF1058_RS14610 at 1159.226 kb on + strand, within HMPREF1058_RS14610 at 1159.244 kb on + strand, within HMPREF1058_RS14610 at 1159.246 kb on + strand, within HMPREF1058_RS14610 at 1159.247 kb on - strand, within HMPREF1058_RS14610 at 1159.302 kb on + strand, within HMPREF1058_RS14610 at 1159.303 kb on - strand at 1159.303 kb on - strand, within HMPREF1058_RS14610 at 1159.405 kb on - strand, within HMPREF1058_RS14610 at 1159.488 kb on + strand, within HMPREF1058_RS14610 at 1159.489 kb on - strand, within HMPREF1058_RS14610 at 1159.489 kb on - strand, within HMPREF1058_RS14610 at 1159.500 kb on - strand, within HMPREF1058_RS14610 at 1159.585 kb on + strand, within HMPREF1058_RS14610 at 1159.585 kb on + strand, within HMPREF1058_RS14610 at 1159.586 kb on - strand, within HMPREF1058_RS14610 at 1159.613 kb on + strand, within HMPREF1058_RS14610 at 1159.613 kb on + strand, within HMPREF1058_RS14610 at 1159.614 kb on - strand, within HMPREF1058_RS14610 at 1159.617 kb on + strand, within HMPREF1058_RS14610 at 1159.618 kb on - strand, within HMPREF1058_RS14610 at 1159.618 kb on - strand, within HMPREF1058_RS14610 at 1159.618 kb on - strand, within HMPREF1058_RS14610 at 1159.618 kb on - strand, within HMPREF1058_RS14610 at 1159.625 kb on - strand, within HMPREF1058_RS14610 at 1159.626 kb on + strand, within HMPREF1058_RS14610 at 1159.662 kb on + strand, within HMPREF1058_RS14610 at 1159.829 kb on - strand, within HMPREF1058_RS14610 at 1159.900 kb on - strand at 1159.900 kb on - strand at 1159.900 kb on - strand at 1159.934 kb on - strand at 1159.934 kb on - strand at 1160.003 kb on - strand at 1160.003 kb on - strand at 1160.074 kb on + strand at 1160.074 kb on + strand at 1160.074 kb on + strand at 1160.075 kb on - strand at 1160.075 kb on - strand at 1160.087 kb on + strand at 1160.088 kb on - strand at 1160.094 kb on + strand, within HMPREF1058_RS14605 at 1160.094 kb on + strand, within HMPREF1058_RS14605 at 1160.094 kb on + strand, within HMPREF1058_RS14605 at 1160.099 kb on - strand, within HMPREF1058_RS14605 at 1160.100 kb on + strand, within HMPREF1058_RS14605 at 1160.100 kb on + strand, within HMPREF1058_RS14605 at 1160.101 kb on + strand, within HMPREF1058_RS14605 at 1160.101 kb on - strand, within HMPREF1058_RS14605 at 1160.101 kb on - strand, within HMPREF1058_RS14605 at 1160.101 kb on - strand, within HMPREF1058_RS14605 at 1160.101 kb on - strand, within HMPREF1058_RS14605 at 1160.205 kb on - strand, within HMPREF1058_RS14605 at 1160.272 kb on + strand, within HMPREF1058_RS14605 at 1160.311 kb on + strand, within HMPREF1058_RS14605 at 1160.313 kb on + strand, within HMPREF1058_RS14605 at 1160.358 kb on + strand, within HMPREF1058_RS14605 at 1160.404 kb on + strand, within HMPREF1058_RS14605 at 1160.405 kb on - strand, within HMPREF1058_RS14605 at 1160.405 kb on - strand, within HMPREF1058_RS14605 at 1160.405 kb on - strand, within HMPREF1058_RS14605 at 1160.416 kb on + strand, within HMPREF1058_RS14605 at 1160.416 kb on + strand, within HMPREF1058_RS14605 at 1160.416 kb on + strand, within HMPREF1058_RS14605 at 1160.416 kb on + strand, within HMPREF1058_RS14605 at 1160.417 kb on - strand, within HMPREF1058_RS14605 at 1160.481 kb on - strand at 1160.550 kb on - strand at 1160.550 kb on - strand at 1160.588 kb on + strand at 1160.741 kb on + strand, within HMPREF1058_RS14600 at 1160.838 kb on - strand, within HMPREF1058_RS14600 at 1160.893 kb on - strand, within HMPREF1058_RS14600 at 1160.897 kb on + strand, within HMPREF1058_RS14600 at 1160.963 kb on + strand, within HMPREF1058_RS14600 at 1160.964 kb on - strand at 1160.973 kb on - strand at 1161.002 kb on + strand, within HMPREF1058_RS14600
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 1,157,753 - -0.6 1,157,769 + +0.7 1,157,769 + +1.2 1,157,775 + +0.7 1,157,785 + -1.1 1,157,786 - HMPREF1058_RS20515 0.10 +2.9 1,157,816 + HMPREF1058_RS20515 0.14 +3.6 1,157,838 + HMPREF1058_RS20515 0.17 -0.8 1,157,838 + HMPREF1058_RS20515 0.17 -0.6 1,157,839 - HMPREF1058_RS20515 0.17 -0.2 1,157,903 - HMPREF1058_RS20515 0.26 -0.3 1,157,903 - HMPREF1058_RS20515 0.26 -0.5 1,157,911 - HMPREF1058_RS20515 0.27 +1.5 1,158,000 - HMPREF1058_RS20515 0.38 -0.6 1,158,163 + HMPREF1058_RS20515 0.60 -1.0 1,158,164 - HMPREF1058_RS20515 0.60 +1.2 1,158,248 + HMPREF1058_RS20515 0.71 +2.2 1,158,340 + HMPREF1058_RS20515 0.83 -0.5 1,158,340 + HMPREF1058_RS20515 0.83 +2.2 1,158,340 + HMPREF1058_RS20515 0.83 +0.5 1,158,359 + HMPREF1058_RS20515 0.86 -0.2 1,158,359 + HMPREF1058_RS20515 0.86 +1.7 1,158,371 - HMPREF1058_RS20515 0.88 +1.7 1,158,396 + +0.7 1,158,397 - -0.5 1,158,493 - +0.8 1,158,531 + +0.4 1,158,534 + -0.3 1,158,535 - +1.8 1,158,541 + +1.2 1,158,577 - -1.3 1,158,577 - -0.9 1,158,593 + +0.4 1,158,594 - -0.2 1,158,611 + -1.4 1,158,611 + -0.9 1,158,616 + -0.7 1,158,621 + -0.7 1,158,622 - -0.3 1,158,637 + -1.1 1,158,637 + -2.1 1,158,638 - -2.4 1,158,642 - -1.1 1,158,661 + -0.4 1,158,666 - +1.4 1,158,666 - -0.4 1,158,675 + -0.7 1,158,675 + +0.9 1,158,681 + +1.5 1,158,734 + -1.0 1,158,752 + -2.8 1,158,752 + -0.9 1,158,775 - -3.2 1,158,848 + +0.3 1,158,849 - -0.2 1,158,849 - -0.6 1,158,927 - HMPREF1058_RS14610 0.14 -3.1 1,158,927 - HMPREF1058_RS14610 0.14 -0.7 1,158,928 + HMPREF1058_RS14610 0.14 +0.0 1,158,951 + HMPREF1058_RS14610 0.16 -1.7 1,158,957 - HMPREF1058_RS14610 0.16 +3.4 1,158,985 + HMPREF1058_RS14610 0.18 -0.9 1,158,986 - HMPREF1058_RS14610 0.18 -2.4 1,158,992 + HMPREF1058_RS14610 0.19 -3.2 1,158,993 - HMPREF1058_RS14610 0.19 -1.0 1,159,048 - HMPREF1058_RS14610 0.23 -3.5 1,159,052 - HMPREF1058_RS14610 0.24 -2.6 1,159,077 + HMPREF1058_RS14610 0.26 -3.6 1,159,077 + HMPREF1058_RS14610 0.26 -2.0 1,159,077 + HMPREF1058_RS14610 0.26 -4.0 1,159,077 + HMPREF1058_RS14610 0.26 -1.8 1,159,078 - HMPREF1058_RS14610 0.26 -3.4 1,159,078 - HMPREF1058_RS14610 0.26 -1.4 1,159,082 + HMPREF1058_RS14610 0.26 -2.2 1,159,082 + HMPREF1058_RS14610 0.26 -2.2 1,159,093 + HMPREF1058_RS14610 0.27 -1.1 1,159,226 + HMPREF1058_RS14610 0.37 -3.1 1,159,244 + HMPREF1058_RS14610 0.39 -1.8 1,159,246 + HMPREF1058_RS14610 0.39 -3.4 1,159,247 - HMPREF1058_RS14610 0.39 -0.7 1,159,302 + HMPREF1058_RS14610 0.43 +0.0 1,159,303 - +1.0 1,159,303 - HMPREF1058_RS14610 0.43 -4.2 1,159,405 - HMPREF1058_RS14610 0.51 +3.4 1,159,488 + HMPREF1058_RS14610 0.58 +1.4 1,159,489 - HMPREF1058_RS14610 0.58 -2.6 1,159,489 - HMPREF1058_RS14610 0.58 -1.9 1,159,500 - HMPREF1058_RS14610 0.59 -2.3 1,159,585 + HMPREF1058_RS14610 0.66 -1.9 1,159,585 + HMPREF1058_RS14610 0.66 -5.0 1,159,586 - HMPREF1058_RS14610 0.66 -3.2 1,159,613 + HMPREF1058_RS14610 0.68 -1.6 1,159,613 + HMPREF1058_RS14610 0.68 -2.7 1,159,614 - HMPREF1058_RS14610 0.68 -3.7 1,159,617 + HMPREF1058_RS14610 0.68 -3.2 1,159,618 - HMPREF1058_RS14610 0.68 -3.9 1,159,618 - HMPREF1058_RS14610 0.68 -4.8 1,159,618 - HMPREF1058_RS14610 0.68 -1.0 1,159,618 - HMPREF1058_RS14610 0.68 -4.0 1,159,625 - HMPREF1058_RS14610 0.69 -2.6 1,159,626 + HMPREF1058_RS14610 0.69 +1.5 1,159,662 + HMPREF1058_RS14610 0.72 -2.9 1,159,829 - HMPREF1058_RS14610 0.85 -2.7 1,159,900 - -2.6 1,159,900 - -0.8 1,159,900 - -3.5 1,159,934 - +0.7 1,159,934 - -1.5 1,160,003 - -3.4 1,160,003 - -0.5 1,160,074 + +0.1 1,160,074 + +1.2 1,160,074 + -1.1 1,160,075 - -0.7 1,160,075 - -2.5 1,160,087 + -2.1 1,160,088 - +2.4 1,160,094 + HMPREF1058_RS14605 0.11 -1.4 1,160,094 + HMPREF1058_RS14605 0.11 +0.6 1,160,094 + HMPREF1058_RS14605 0.11 +4.4 1,160,099 - HMPREF1058_RS14605 0.12 +1.4 1,160,100 + HMPREF1058_RS14605 0.12 +2.0 1,160,100 + HMPREF1058_RS14605 0.12 +0.3 1,160,101 + HMPREF1058_RS14605 0.13 +0.5 1,160,101 - HMPREF1058_RS14605 0.13 +1.7 1,160,101 - HMPREF1058_RS14605 0.13 +0.4 1,160,101 - HMPREF1058_RS14605 0.13 +2.6 1,160,101 - HMPREF1058_RS14605 0.13 -0.4 1,160,205 - HMPREF1058_RS14605 0.34 +2.0 1,160,272 + HMPREF1058_RS14605 0.47 +1.4 1,160,311 + HMPREF1058_RS14605 0.55 +2.4 1,160,313 + HMPREF1058_RS14605 0.56 +0.9 1,160,358 + HMPREF1058_RS14605 0.65 +0.8 1,160,404 + HMPREF1058_RS14605 0.74 +1.2 1,160,405 - HMPREF1058_RS14605 0.74 +1.5 1,160,405 - HMPREF1058_RS14605 0.74 -0.3 1,160,405 - HMPREF1058_RS14605 0.74 +0.5 1,160,416 + HMPREF1058_RS14605 0.77 +1.0 1,160,416 + HMPREF1058_RS14605 0.77 +4.4 1,160,416 + HMPREF1058_RS14605 0.77 +0.6 1,160,416 + HMPREF1058_RS14605 0.77 +1.6 1,160,417 - HMPREF1058_RS14605 0.77 +1.0 1,160,481 - +0.7 1,160,550 - -0.7 1,160,550 - -0.2 1,160,588 + -2.1 1,160,741 + HMPREF1058_RS14600 0.27 -0.0 1,160,838 - HMPREF1058_RS14600 0.41 -0.1 1,160,893 - HMPREF1058_RS14600 0.50 -0.4 1,160,897 + HMPREF1058_RS14600 0.50 -0.2 1,160,963 + HMPREF1058_RS14600 0.60 -2.9 1,160,964 - +1.0 1,160,973 - +3.4 1,161,002 + HMPREF1058_RS14600 0.66 -0.9
Or see this region's nucleotide sequence