Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS20225 and HMPREF1058_RS13675 are separated by 122 nucleotides HMPREF1058_RS13675 and HMPREF1058_RS20540 are separated by 35 nucleotides HMPREF1058_RS20540 and HMPREF1058_RS13670 are separated by 12 nucleotides
HMPREF1058_RS20225: HMPREF1058_RS20225 - hypothetical protein, at 1,402,374 to 1,402,811
_RS20225
HMPREF1058_RS13675: HMPREF1058_RS13675 - hypothetical protein, at 1,402,934 to 1,403,296
_RS13675
HMPREF1058_RS20540: HMPREF1058_RS20540 - hypothetical protein, at 1,403,332 to 1,403,610
_RS20540
HMPREF1058_RS13670: HMPREF1058_RS13670 - TonB-dependent receptor, at 1,403,623 to 1,405,956
_RS13670
Position (kb)
1402
1403
1404 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1401.967 kb on + strand at 1401.967 kb on + strand at 1401.968 kb on - strand at 1401.968 kb on - strand at 1401.968 kb on - strand at 1401.968 kb on - strand at 1401.968 kb on - strand at 1402.000 kb on + strand at 1402.001 kb on - strand at 1402.101 kb on + strand at 1402.101 kb on + strand at 1402.101 kb on + strand at 1402.102 kb on - strand at 1402.103 kb on + strand at 1402.104 kb on - strand at 1402.198 kb on + strand at 1402.201 kb on - strand at 1402.269 kb on + strand at 1402.345 kb on + strand at 1402.382 kb on - strand at 1402.413 kb on - strand at 1402.430 kb on + strand, within HMPREF1058_RS20225 at 1402.457 kb on + strand, within HMPREF1058_RS20225 at 1402.561 kb on - strand, within HMPREF1058_RS20225 at 1402.628 kb on + strand, within HMPREF1058_RS20225 at 1402.631 kb on - strand, within HMPREF1058_RS20225 at 1402.741 kb on + strand, within HMPREF1058_RS20225 at 1402.745 kb on - strand, within HMPREF1058_RS20225 at 1402.748 kb on + strand, within HMPREF1058_RS20225 at 1402.774 kb on + strand at 1402.835 kb on - strand at 1402.842 kb on + strand at 1402.892 kb on + strand at 1402.905 kb on - strand at 1402.915 kb on - strand at 1402.915 kb on - strand at 1402.915 kb on - strand at 1402.918 kb on - strand at 1402.923 kb on + strand at 1402.935 kb on + strand at 1402.987 kb on + strand, within HMPREF1058_RS13675 at 1403.001 kb on - strand, within HMPREF1058_RS13675 at 1403.080 kb on - strand, within HMPREF1058_RS13675 at 1403.080 kb on - strand, within HMPREF1058_RS13675 at 1403.152 kb on + strand, within HMPREF1058_RS13675 at 1403.155 kb on + strand, within HMPREF1058_RS13675 at 1403.169 kb on + strand, within HMPREF1058_RS13675 at 1403.170 kb on - strand, within HMPREF1058_RS13675 at 1403.170 kb on - strand, within HMPREF1058_RS13675 at 1403.170 kb on - strand, within HMPREF1058_RS13675 at 1403.267 kb on + strand at 1403.268 kb on - strand at 1403.268 kb on - strand at 1403.320 kb on + strand at 1403.322 kb on + strand at 1403.333 kb on + strand at 1403.422 kb on - strand, within HMPREF1058_RS20540 at 1403.428 kb on + strand, within HMPREF1058_RS20540 at 1403.526 kb on + strand, within HMPREF1058_RS20540 at 1403.532 kb on - strand, within HMPREF1058_RS20540 at 1403.534 kb on + strand, within HMPREF1058_RS20540 at 1403.619 kb on + strand at 1403.619 kb on + strand at 1403.707 kb on + strand at 1403.734 kb on - strand at 1403.785 kb on - strand at 1403.798 kb on - strand at 1403.820 kb on + strand at 1403.865 kb on + strand, within HMPREF1058_RS13670 at 1403.881 kb on - strand, within HMPREF1058_RS13670 at 1403.967 kb on + strand, within HMPREF1058_RS13670 at 1403.967 kb on + strand, within HMPREF1058_RS13670 at 1403.967 kb on + strand, within HMPREF1058_RS13670 at 1403.979 kb on + strand, within HMPREF1058_RS13670 at 1404.032 kb on + strand, within HMPREF1058_RS13670 at 1404.033 kb on - strand, within HMPREF1058_RS13670 at 1404.080 kb on + strand, within HMPREF1058_RS13670 at 1404.081 kb on - strand, within HMPREF1058_RS13670 at 1404.102 kb on - strand, within HMPREF1058_RS13670 at 1404.163 kb on - strand, within HMPREF1058_RS13670 at 1404.163 kb on - strand, within HMPREF1058_RS13670 at 1404.249 kb on + strand, within HMPREF1058_RS13670 at 1404.250 kb on - strand, within HMPREF1058_RS13670 at 1404.251 kb on + strand, within HMPREF1058_RS13670 at 1404.274 kb on - strand, within HMPREF1058_RS13670
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 1,401,967 + +0.8 1,401,967 + -0.3 1,401,968 - -1.4 1,401,968 - -0.1 1,401,968 - -0.6 1,401,968 - -0.1 1,401,968 - -1.5 1,402,000 + -0.1 1,402,001 - -1.2 1,402,101 + -1.5 1,402,101 + +3.4 1,402,101 + +0.9 1,402,102 - -0.4 1,402,103 + -0.3 1,402,104 - -0.4 1,402,198 + -1.3 1,402,201 - +0.5 1,402,269 + +0.4 1,402,345 + -0.4 1,402,382 - -0.7 1,402,413 - -1.0 1,402,430 + HMPREF1058_RS20225 0.13 +0.4 1,402,457 + HMPREF1058_RS20225 0.19 -0.6 1,402,561 - HMPREF1058_RS20225 0.43 +0.0 1,402,628 + HMPREF1058_RS20225 0.58 -1.4 1,402,631 - HMPREF1058_RS20225 0.59 -0.0 1,402,741 + HMPREF1058_RS20225 0.84 -0.3 1,402,745 - HMPREF1058_RS20225 0.85 +0.6 1,402,748 + HMPREF1058_RS20225 0.85 +0.8 1,402,774 + +0.1 1,402,835 - +0.3 1,402,842 + -0.6 1,402,892 + -0.6 1,402,905 - +0.2 1,402,915 - -1.2 1,402,915 - -1.5 1,402,915 - -0.9 1,402,918 - -2.0 1,402,923 + -3.2 1,402,935 + -0.8 1,402,987 + HMPREF1058_RS13675 0.15 -0.2 1,403,001 - HMPREF1058_RS13675 0.18 -1.2 1,403,080 - HMPREF1058_RS13675 0.40 -1.0 1,403,080 - HMPREF1058_RS13675 0.40 -0.8 1,403,152 + HMPREF1058_RS13675 0.60 +1.2 1,403,155 + HMPREF1058_RS13675 0.61 +0.5 1,403,169 + HMPREF1058_RS13675 0.65 -0.3 1,403,170 - HMPREF1058_RS13675 0.65 -1.4 1,403,170 - HMPREF1058_RS13675 0.65 +0.1 1,403,170 - HMPREF1058_RS13675 0.65 -2.6 1,403,267 + -1.3 1,403,268 - -0.8 1,403,268 - +0.3 1,403,320 + -1.3 1,403,322 + -0.6 1,403,333 + -1.0 1,403,422 - HMPREF1058_RS20540 0.32 +0.2 1,403,428 + HMPREF1058_RS20540 0.34 -0.7 1,403,526 + HMPREF1058_RS20540 0.70 +0.1 1,403,532 - HMPREF1058_RS20540 0.72 -0.2 1,403,534 + HMPREF1058_RS20540 0.72 -0.2 1,403,619 + +0.3 1,403,619 + +1.1 1,403,707 + -0.5 1,403,734 - -1.5 1,403,785 - +1.9 1,403,798 - +1.3 1,403,820 + +0.2 1,403,865 + HMPREF1058_RS13670 0.10 -0.1 1,403,881 - HMPREF1058_RS13670 0.11 -0.3 1,403,967 + HMPREF1058_RS13670 0.15 -0.7 1,403,967 + HMPREF1058_RS13670 0.15 -0.8 1,403,967 + HMPREF1058_RS13670 0.15 +0.4 1,403,979 + HMPREF1058_RS13670 0.15 -1.9 1,404,032 + HMPREF1058_RS13670 0.18 -1.8 1,404,033 - HMPREF1058_RS13670 0.18 -0.8 1,404,080 + HMPREF1058_RS13670 0.20 -1.5 1,404,081 - HMPREF1058_RS13670 0.20 +1.1 1,404,102 - HMPREF1058_RS13670 0.21 -2.3 1,404,163 - HMPREF1058_RS13670 0.23 +2.6 1,404,163 - HMPREF1058_RS13670 0.23 +0.4 1,404,249 + HMPREF1058_RS13670 0.27 -0.4 1,404,250 - HMPREF1058_RS13670 0.27 +2.8 1,404,251 + HMPREF1058_RS13670 0.27 -1.2 1,404,274 - HMPREF1058_RS13670 0.28 -2.5
Or see this region's nucleotide sequence