Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS12500 and HMPREF1058_RS12495 are separated by 36 nucleotides HMPREF1058_RS12495 and HMPREF1058_RS12490 are separated by 124 nucleotides HMPREF1058_RS12490 and HMPREF1058_RS12485 are separated by 83 nucleotides
HMPREF1058_RS12500: HMPREF1058_RS12500 - phosphatase PAP2 family protein, at 94,042 to 94,698
_RS12500
HMPREF1058_RS12495: HMPREF1058_RS12495 - glycine cleavage system protein GcvH, at 94,735 to 95,115
_RS12495
HMPREF1058_RS12490: HMPREF1058_RS12490 - 5-(carboxyamino)imidazole ribonucleotide mutase, at 95,240 to 95,749
_RS12490
HMPREF1058_RS12485: HMPREF1058_RS12485 - 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase, at 95,833 to 97,677
_RS12485
Position (kb) 95
96 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 94.271 kb on - strand, within HMPREF1058_RS12500 at 94.339 kb on + strand, within HMPREF1058_RS12500 at 94.350 kb on + strand, within HMPREF1058_RS12500 at 94.386 kb on + strand, within HMPREF1058_RS12500 at 94.387 kb on - strand, within HMPREF1058_RS12500 at 94.408 kb on - strand, within HMPREF1058_RS12500 at 94.537 kb on - strand, within HMPREF1058_RS12500 at 94.617 kb on - strand, within HMPREF1058_RS12500 at 94.627 kb on - strand, within HMPREF1058_RS12500 at 94.627 kb on - strand, within HMPREF1058_RS12500 at 94.631 kb on + strand, within HMPREF1058_RS12500 at 94.632 kb on - strand, within HMPREF1058_RS12500 at 94.634 kb on + strand at 94.754 kb on + strand at 94.787 kb on + strand, within HMPREF1058_RS12495 at 94.799 kb on + strand at 94.799 kb on + strand, within HMPREF1058_RS12495 at 94.799 kb on + strand, within HMPREF1058_RS12495 at 94.799 kb on + strand, within HMPREF1058_RS12495 at 94.804 kb on + strand, within HMPREF1058_RS12495 at 94.856 kb on + strand, within HMPREF1058_RS12495 at 95.037 kb on + strand, within HMPREF1058_RS12495 at 95.102 kb on + strand at 95.118 kb on + strand at 95.118 kb on + strand at 95.118 kb on + strand at 95.118 kb on + strand at 95.214 kb on + strand at 95.328 kb on + strand, within HMPREF1058_RS12490 at 95.571 kb on + strand, within HMPREF1058_RS12490 at 95.571 kb on + strand, within HMPREF1058_RS12490 at 95.747 kb on + strand at 95.771 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 94,271 - HMPREF1058_RS12500 0.35 -0.3 94,339 + HMPREF1058_RS12500 0.45 +0.6 94,350 + HMPREF1058_RS12500 0.47 -1.4 94,386 + HMPREF1058_RS12500 0.52 -2.1 94,387 - HMPREF1058_RS12500 0.53 +1.9 94,408 - HMPREF1058_RS12500 0.56 -0.9 94,537 - HMPREF1058_RS12500 0.75 -1.6 94,617 - HMPREF1058_RS12500 0.88 +0.1 94,627 - HMPREF1058_RS12500 0.89 -0.8 94,627 - HMPREF1058_RS12500 0.89 +0.0 94,631 + HMPREF1058_RS12500 0.90 -0.8 94,632 - HMPREF1058_RS12500 0.90 -1.2 94,634 + -0.3 94,754 + -1.6 94,787 + HMPREF1058_RS12495 0.14 -0.7 94,799 + +0.6 94,799 + HMPREF1058_RS12495 0.17 -0.4 94,799 + HMPREF1058_RS12495 0.17 -1.1 94,799 + HMPREF1058_RS12495 0.17 -0.9 94,804 + HMPREF1058_RS12495 0.18 +1.0 94,856 + HMPREF1058_RS12495 0.32 -0.7 95,037 + HMPREF1058_RS12495 0.79 -0.2 95,102 + +1.0 95,118 + -0.1 95,118 + -1.0 95,118 + +0.4 95,118 + -1.0 95,214 + -0.6 95,328 + HMPREF1058_RS12490 0.17 -2.2 95,571 + HMPREF1058_RS12490 0.65 -3.4 95,571 + HMPREF1058_RS12490 0.65 -1.8 95,747 + -1.4 95,771 + -1.5
Or see this region's nucleotide sequence