Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS11305 and HMPREF1058_RS11300 are separated by 12 nucleotides HMPREF1058_RS11300 and HMPREF1058_RS11295 are separated by 2 nucleotides HMPREF1058_RS11295 and HMPREF1058_RS11290 are separated by 137 nucleotides HMPREF1058_RS11290 and HMPREF1058_RS11285 are separated by 9 nucleotides HMPREF1058_RS11285 and HMPREF1058_RS11280 overlap by 1 nucleotides
HMPREF1058_RS11305: HMPREF1058_RS11305 - DUF3098 domain-containing protein, at 406,232 to 406,477
_RS11305
HMPREF1058_RS11300: HMPREF1058_RS11300 - ABC transporter permease, at 406,490 to 407,359
_RS11300
HMPREF1058_RS11295: HMPREF1058_RS11295 - methyltransferase domain-containing protein, at 407,362 to 408,258
_RS11295
HMPREF1058_RS11290: HMPREF1058_RS11290 - PspC domain-containing protein, at 408,396 to 408,632
_RS11290
HMPREF1058_RS11285: HMPREF1058_RS11285 - FKBP-type peptidyl-prolyl cis-trans isomerase, at 408,642 to 409,043
_RS11285
HMPREF1058_RS11280: HMPREF1058_RS11280 - tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB, at 409,043 to 410,413
_RS11280
Position (kb)
407
408
409 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 406.478 kb on - strand at 406.486 kb on - strand at 406.486 kb on - strand at 406.501 kb on - strand at 406.501 kb on - strand at 406.501 kb on - strand at 406.528 kb on - strand at 406.544 kb on - strand at 406.544 kb on - strand at 406.546 kb on - strand at 406.546 kb on - strand at 406.546 kb on - strand at 406.546 kb on - strand at 406.546 kb on - strand at 406.622 kb on - strand, within HMPREF1058_RS11300 at 406.645 kb on - strand, within HMPREF1058_RS11300 at 406.650 kb on - strand, within HMPREF1058_RS11300 at 406.716 kb on - strand, within HMPREF1058_RS11300 at 406.823 kb on - strand, within HMPREF1058_RS11300 at 406.944 kb on - strand, within HMPREF1058_RS11300 at 407.115 kb on - strand, within HMPREF1058_RS11300 at 407.117 kb on - strand at 407.117 kb on - strand, within HMPREF1058_RS11300 at 407.117 kb on - strand, within HMPREF1058_RS11300 at 407.233 kb on - strand, within HMPREF1058_RS11300 at 407.479 kb on - strand, within HMPREF1058_RS11295 at 407.481 kb on - strand, within HMPREF1058_RS11295 at 407.597 kb on - strand, within HMPREF1058_RS11295 at 407.616 kb on - strand, within HMPREF1058_RS11295 at 407.652 kb on - strand, within HMPREF1058_RS11295 at 407.935 kb on + strand, within HMPREF1058_RS11295 at 407.935 kb on + strand at 407.936 kb on - strand, within HMPREF1058_RS11295 at 407.978 kb on - strand, within HMPREF1058_RS11295 at 408.003 kb on + strand, within HMPREF1058_RS11295 at 408.004 kb on - strand, within HMPREF1058_RS11295 at 408.004 kb on - strand, within HMPREF1058_RS11295 at 408.006 kb on - strand, within HMPREF1058_RS11295 at 408.006 kb on - strand, within HMPREF1058_RS11295 at 408.026 kb on - strand, within HMPREF1058_RS11295 at 408.067 kb on - strand, within HMPREF1058_RS11295 at 408.069 kb on - strand at 408.084 kb on - strand, within HMPREF1058_RS11295 at 408.180 kb on - strand at 408.238 kb on - strand at 408.251 kb on - strand at 408.327 kb on - strand at 408.362 kb on + strand at 408.497 kb on + strand, within HMPREF1058_RS11290 at 408.498 kb on - strand, within HMPREF1058_RS11290 at 408.548 kb on + strand, within HMPREF1058_RS11290 at 408.550 kb on + strand, within HMPREF1058_RS11290 at 408.550 kb on + strand, within HMPREF1058_RS11290 at 408.551 kb on - strand, within HMPREF1058_RS11290 at 408.551 kb on - strand, within HMPREF1058_RS11290 at 408.567 kb on - strand, within HMPREF1058_RS11290 at 408.643 kb on + strand at 408.681 kb on - strand at 408.728 kb on + strand, within HMPREF1058_RS11285 at 408.728 kb on + strand, within HMPREF1058_RS11285 at 408.728 kb on + strand, within HMPREF1058_RS11285 at 408.729 kb on - strand, within HMPREF1058_RS11285 at 408.729 kb on - strand, within HMPREF1058_RS11285 at 408.729 kb on - strand, within HMPREF1058_RS11285 at 408.729 kb on - strand, within HMPREF1058_RS11285 at 408.729 kb on - strand, within HMPREF1058_RS11285 at 408.729 kb on - strand, within HMPREF1058_RS11285 at 408.729 kb on - strand, within HMPREF1058_RS11285 at 408.731 kb on - strand, within HMPREF1058_RS11285 at 408.739 kb on + strand, within HMPREF1058_RS11285 at 408.740 kb on - strand, within HMPREF1058_RS11285 at 408.753 kb on - strand, within HMPREF1058_RS11285 at 408.760 kb on - strand, within HMPREF1058_RS11285 at 408.767 kb on + strand, within HMPREF1058_RS11285 at 408.796 kb on + strand, within HMPREF1058_RS11285 at 408.796 kb on - strand, within HMPREF1058_RS11285 at 408.846 kb on - strand, within HMPREF1058_RS11285 at 408.896 kb on + strand, within HMPREF1058_RS11285 at 408.896 kb on + strand, within HMPREF1058_RS11285 at 408.897 kb on - strand, within HMPREF1058_RS11285 at 408.908 kb on + strand, within HMPREF1058_RS11285 at 408.940 kb on - strand, within HMPREF1058_RS11285 at 408.944 kb on + strand, within HMPREF1058_RS11285 at 408.957 kb on - strand, within HMPREF1058_RS11285 at 408.957 kb on - strand, within HMPREF1058_RS11285 at 408.986 kb on + strand, within HMPREF1058_RS11285 at 409.004 kb on - strand at 409.023 kb on - strand at 409.025 kb on - strand at 409.094 kb on + strand at 409.095 kb on - strand at 409.095 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 406,478 - -0.3 406,486 - +1.8 406,486 - +0.6 406,501 - +0.5 406,501 - +0.2 406,501 - -0.1 406,528 - -0.1 406,544 - +0.6 406,544 - -0.7 406,546 - +0.2 406,546 - +0.2 406,546 - +0.7 406,546 - -0.4 406,546 - -1.2 406,622 - HMPREF1058_RS11300 0.15 -1.6 406,645 - HMPREF1058_RS11300 0.18 -0.2 406,650 - HMPREF1058_RS11300 0.18 +1.7 406,716 - HMPREF1058_RS11300 0.26 -0.2 406,823 - HMPREF1058_RS11300 0.38 -1.3 406,944 - HMPREF1058_RS11300 0.52 -0.8 407,115 - HMPREF1058_RS11300 0.72 -1.6 407,117 - +1.9 407,117 - HMPREF1058_RS11300 0.72 -0.1 407,117 - HMPREF1058_RS11300 0.72 +0.6 407,233 - HMPREF1058_RS11300 0.85 -0.4 407,479 - HMPREF1058_RS11295 0.13 -1.9 407,481 - HMPREF1058_RS11295 0.13 -0.9 407,597 - HMPREF1058_RS11295 0.26 +0.7 407,616 - HMPREF1058_RS11295 0.28 -2.0 407,652 - HMPREF1058_RS11295 0.32 -0.6 407,935 + HMPREF1058_RS11295 0.64 +0.8 407,935 + +1.0 407,936 - HMPREF1058_RS11295 0.64 -1.3 407,978 - HMPREF1058_RS11295 0.69 -1.3 408,003 + HMPREF1058_RS11295 0.71 +0.2 408,004 - HMPREF1058_RS11295 0.72 -0.1 408,004 - HMPREF1058_RS11295 0.72 -2.1 408,006 - HMPREF1058_RS11295 0.72 -2.0 408,006 - HMPREF1058_RS11295 0.72 +0.3 408,026 - HMPREF1058_RS11295 0.74 -0.5 408,067 - HMPREF1058_RS11295 0.79 -2.1 408,069 - +1.0 408,084 - HMPREF1058_RS11295 0.80 -1.5 408,180 - +0.5 408,238 - -2.5 408,251 - -0.5 408,327 - -1.3 408,362 + +1.0 408,497 + HMPREF1058_RS11290 0.43 -2.6 408,498 - HMPREF1058_RS11290 0.43 -1.1 408,548 + HMPREF1058_RS11290 0.64 +0.8 408,550 + HMPREF1058_RS11290 0.65 -1.2 408,550 + HMPREF1058_RS11290 0.65 -1.2 408,551 - HMPREF1058_RS11290 0.65 -1.0 408,551 - HMPREF1058_RS11290 0.65 -1.7 408,567 - HMPREF1058_RS11290 0.72 -0.5 408,643 + -1.1 408,681 - -1.5 408,728 + HMPREF1058_RS11285 0.21 -0.5 408,728 + HMPREF1058_RS11285 0.21 -1.6 408,728 + HMPREF1058_RS11285 0.21 -2.6 408,729 - HMPREF1058_RS11285 0.22 -1.1 408,729 - HMPREF1058_RS11285 0.22 -2.0 408,729 - HMPREF1058_RS11285 0.22 -2.3 408,729 - HMPREF1058_RS11285 0.22 -0.6 408,729 - HMPREF1058_RS11285 0.22 +0.2 408,729 - HMPREF1058_RS11285 0.22 +1.5 408,729 - HMPREF1058_RS11285 0.22 -0.6 408,731 - HMPREF1058_RS11285 0.22 -1.4 408,739 + HMPREF1058_RS11285 0.24 -0.3 408,740 - HMPREF1058_RS11285 0.24 -0.7 408,753 - HMPREF1058_RS11285 0.28 +3.4 408,760 - HMPREF1058_RS11285 0.29 -1.7 408,767 + HMPREF1058_RS11285 0.31 -1.1 408,796 + HMPREF1058_RS11285 0.38 -1.7 408,796 - HMPREF1058_RS11285 0.38 +1.3 408,846 - HMPREF1058_RS11285 0.51 -0.5 408,896 + HMPREF1058_RS11285 0.63 -0.1 408,896 + HMPREF1058_RS11285 0.63 -1.6 408,897 - HMPREF1058_RS11285 0.63 +1.3 408,908 + HMPREF1058_RS11285 0.66 +0.1 408,940 - HMPREF1058_RS11285 0.74 -1.3 408,944 + HMPREF1058_RS11285 0.75 +1.6 408,957 - HMPREF1058_RS11285 0.78 -0.7 408,957 - HMPREF1058_RS11285 0.78 -0.3 408,986 + HMPREF1058_RS11285 0.86 -1.4 409,004 - -0.7 409,023 - +0.2 409,025 - -1.6 409,094 + +1.0 409,095 - -0.9 409,095 - +0.3
Or see this region's nucleotide sequence