Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS07200 and HMPREF1058_RS07195 overlap by 4 nucleotides HMPREF1058_RS07195 and HMPREF1058_RS07190 are separated by 12 nucleotides HMPREF1058_RS07190 and HMPREF1058_RS07185 are separated by 38 nucleotides
HMPREF1058_RS07200: HMPREF1058_RS07200 - tryptophan synthase subunit alpha, at 290,212 to 290,988
_RS07200
HMPREF1058_RS07195: HMPREF1058_RS07195 - phosphoribosylanthranilate isomerase, at 290,985 to 291,602
_RS07195
HMPREF1058_RS07190: HMPREF1058_RS07190 - indole-3-glycerol phosphate synthase TrpC, at 291,615 to 292,403
_RS07190
HMPREF1058_RS07185: HMPREF1058_RS07185 - anthranilate phosphoribosyltransferase, at 292,442 to 293,437
_RS07185
Position (kb) 291
292
293 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 290.723 kb on - strand, within HMPREF1058_RS07200 at 290.863 kb on - strand, within HMPREF1058_RS07200 at 290.908 kb on + strand, within HMPREF1058_RS07200 at 290.908 kb on + strand, within HMPREF1058_RS07200 at 291.015 kb on - strand at 291.173 kb on - strand, within HMPREF1058_RS07195 at 291.248 kb on - strand, within HMPREF1058_RS07195 at 291.257 kb on + strand, within HMPREF1058_RS07195 at 291.340 kb on - strand, within HMPREF1058_RS07195 at 291.384 kb on - strand, within HMPREF1058_RS07195 at 291.496 kb on + strand, within HMPREF1058_RS07195 at 291.589 kb on - strand at 291.638 kb on + strand at 291.877 kb on - strand, within HMPREF1058_RS07190 at 291.877 kb on - strand, within HMPREF1058_RS07190 at 291.879 kb on - strand, within HMPREF1058_RS07190 at 291.879 kb on - strand, within HMPREF1058_RS07190 at 291.879 kb on - strand, within HMPREF1058_RS07190 at 291.880 kb on + strand, within HMPREF1058_RS07190 at 291.881 kb on - strand, within HMPREF1058_RS07190 at 292.041 kb on + strand, within HMPREF1058_RS07190 at 292.042 kb on - strand, within HMPREF1058_RS07190 at 292.185 kb on - strand, within HMPREF1058_RS07190 at 292.393 kb on - strand at 292.393 kb on - strand at 292.557 kb on - strand at 292.557 kb on - strand, within HMPREF1058_RS07185 at 292.698 kb on - strand, within HMPREF1058_RS07185 at 292.834 kb on - strand, within HMPREF1058_RS07185 at 292.920 kb on - strand, within HMPREF1058_RS07185 at 292.924 kb on + strand, within HMPREF1058_RS07185 at 292.928 kb on + strand, within HMPREF1058_RS07185 at 292.929 kb on - strand, within HMPREF1058_RS07185 at 292.929 kb on - strand, within HMPREF1058_RS07185 at 292.929 kb on - strand, within HMPREF1058_RS07185 at 292.995 kb on + strand, within HMPREF1058_RS07185 at 292.995 kb on - strand, within HMPREF1058_RS07185 at 292.996 kb on - strand, within HMPREF1058_RS07185 at 292.996 kb on - strand, within HMPREF1058_RS07185 at 293.106 kb on + strand, within HMPREF1058_RS07185 at 293.164 kb on + strand, within HMPREF1058_RS07185 at 293.180 kb on - strand, within HMPREF1058_RS07185 at 293.237 kb on + strand, within HMPREF1058_RS07185 at 293.346 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 290,723 - HMPREF1058_RS07200 0.66 -2.4 290,863 - HMPREF1058_RS07200 0.84 -3.2 290,908 + HMPREF1058_RS07200 0.90 -2.5 290,908 + HMPREF1058_RS07200 0.90 -1.7 291,015 - -0.5 291,173 - HMPREF1058_RS07195 0.30 -0.2 291,248 - HMPREF1058_RS07195 0.43 +0.0 291,257 + HMPREF1058_RS07195 0.44 -2.0 291,340 - HMPREF1058_RS07195 0.57 -1.8 291,384 - HMPREF1058_RS07195 0.65 -1.2 291,496 + HMPREF1058_RS07195 0.83 +0.6 291,589 - -1.7 291,638 + -0.7 291,877 - HMPREF1058_RS07190 0.33 +1.2 291,877 - HMPREF1058_RS07190 0.33 -1.1 291,879 - HMPREF1058_RS07190 0.33 -2.2 291,879 - HMPREF1058_RS07190 0.33 -0.2 291,879 - HMPREF1058_RS07190 0.33 -4.0 291,880 + HMPREF1058_RS07190 0.34 -2.5 291,881 - HMPREF1058_RS07190 0.34 -1.0 292,041 + HMPREF1058_RS07190 0.54 -1.7 292,042 - HMPREF1058_RS07190 0.54 -1.2 292,185 - HMPREF1058_RS07190 0.72 -0.5 292,393 - -3.1 292,393 - -2.4 292,557 - +1.2 292,557 - HMPREF1058_RS07185 0.12 -2.0 292,698 - HMPREF1058_RS07185 0.26 -3.1 292,834 - HMPREF1058_RS07185 0.39 -3.0 292,920 - HMPREF1058_RS07185 0.48 -1.8 292,924 + HMPREF1058_RS07185 0.48 -0.9 292,928 + HMPREF1058_RS07185 0.49 -1.8 292,929 - HMPREF1058_RS07185 0.49 -3.3 292,929 - HMPREF1058_RS07185 0.49 +0.5 292,929 - HMPREF1058_RS07185 0.49 -3.0 292,995 + HMPREF1058_RS07185 0.56 +0.4 292,995 - HMPREF1058_RS07185 0.56 +0.0 292,996 - HMPREF1058_RS07185 0.56 -3.5 292,996 - HMPREF1058_RS07185 0.56 -3.0 293,106 + HMPREF1058_RS07185 0.67 +0.0 293,164 + HMPREF1058_RS07185 0.72 +1.0 293,180 - HMPREF1058_RS07185 0.74 +1.0 293,237 + HMPREF1058_RS07185 0.80 -0.4 293,346 + -1.9
Or see this region's nucleotide sequence