Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS06190 and HMPREF1058_RS06185 are separated by 107 nucleotides HMPREF1058_RS06185 and HMPREF1058_RS06180 are separated by 1 nucleotides HMPREF1058_RS06180 and HMPREF1058_RS06175 are separated by 164 nucleotides HMPREF1058_RS06175 and HMPREF1058_RS06170 are separated by 9 nucleotides
HMPREF1058_RS06190: HMPREF1058_RS06190 - aspartate kinase, at 538,864 to 540,183
_RS06190
HMPREF1058_RS06185: HMPREF1058_RS06185 - ATP-binding cassette domain-containing protein, at 540,291 to 541,049
_RS06185
HMPREF1058_RS06180: HMPREF1058_RS06180 - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 541,051 to 541,662
_RS06180
HMPREF1058_RS06175: HMPREF1058_RS06175 - imidazole glycerol phosphate synthase subunit HisF, at 541,827 to 542,582
_RS06175
HMPREF1058_RS06170: HMPREF1058_RS06170 - 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino]imidazole-4- carboxamide isomerase, at 542,592 to 543,311
_RS06170
Position (kb) 541
542 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 540.085 kb on + strand at 540.116 kb on - strand at 540.124 kb on + strand at 540.160 kb on - strand at 540.173 kb on - strand at 540.219 kb on - strand at 540.219 kb on - strand at 540.222 kb on + strand at 540.315 kb on - strand at 540.315 kb on - strand at 540.315 kb on - strand at 540.370 kb on + strand, within HMPREF1058_RS06185 at 540.371 kb on - strand, within HMPREF1058_RS06185 at 540.818 kb on + strand, within HMPREF1058_RS06185 at 540.819 kb on - strand, within HMPREF1058_RS06185 at 540.876 kb on + strand, within HMPREF1058_RS06185 at 540.877 kb on - strand, within HMPREF1058_RS06185 at 540.877 kb on - strand, within HMPREF1058_RS06185 at 540.877 kb on - strand, within HMPREF1058_RS06185 at 540.975 kb on + strand at 540.993 kb on + strand at 541.010 kb on + strand at 541.011 kb on - strand at 541.012 kb on + strand at 541.014 kb on + strand at 541.014 kb on + strand at 541.015 kb on - strand at 541.136 kb on - strand, within HMPREF1058_RS06180 at 541.136 kb on - strand, within HMPREF1058_RS06180 at 541.136 kb on - strand, within HMPREF1058_RS06180 at 541.136 kb on - strand, within HMPREF1058_RS06180 at 541.149 kb on - strand, within HMPREF1058_RS06180 at 541.169 kb on + strand, within HMPREF1058_RS06180 at 541.328 kb on - strand, within HMPREF1058_RS06180 at 541.377 kb on + strand, within HMPREF1058_RS06180 at 541.377 kb on + strand, within HMPREF1058_RS06180 at 541.378 kb on - strand, within HMPREF1058_RS06180 at 541.396 kb on + strand, within HMPREF1058_RS06180 at 541.396 kb on + strand, within HMPREF1058_RS06180 at 541.397 kb on - strand, within HMPREF1058_RS06180 at 541.414 kb on + strand, within HMPREF1058_RS06180 at 541.415 kb on - strand, within HMPREF1058_RS06180 at 541.615 kb on + strand at 541.616 kb on - strand at 541.683 kb on + strand at 541.684 kb on - strand at 541.697 kb on + strand at 541.697 kb on + strand at 541.698 kb on - strand at 541.828 kb on + strand at 541.831 kb on - strand at 541.842 kb on - strand at 542.129 kb on - strand, within HMPREF1058_RS06175 at 542.335 kb on - strand, within HMPREF1058_RS06175 at 542.336 kb on + strand, within HMPREF1058_RS06175 at 542.336 kb on + strand, within HMPREF1058_RS06175 at 542.337 kb on - strand, within HMPREF1058_RS06175 at 542.337 kb on - strand, within HMPREF1058_RS06175 at 542.467 kb on + strand, within HMPREF1058_RS06175 at 542.471 kb on + strand, within HMPREF1058_RS06175 at 542.471 kb on + strand, within HMPREF1058_RS06175 at 542.529 kb on + strand at 542.594 kb on - strand at 542.594 kb on - strand at 542.635 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 540,085 + -1.4 540,116 - -0.0 540,124 + +0.2 540,160 - -0.7 540,173 - -0.1 540,219 - +1.0 540,219 - +1.3 540,222 + +1.2 540,315 - +2.0 540,315 - -0.8 540,315 - +0.6 540,370 + HMPREF1058_RS06185 0.10 -2.0 540,371 - HMPREF1058_RS06185 0.11 +0.3 540,818 + HMPREF1058_RS06185 0.69 +0.7 540,819 - HMPREF1058_RS06185 0.70 +0.2 540,876 + HMPREF1058_RS06185 0.77 -0.3 540,877 - HMPREF1058_RS06185 0.77 -0.4 540,877 - HMPREF1058_RS06185 0.77 -1.6 540,877 - HMPREF1058_RS06185 0.77 +0.8 540,975 + -0.8 540,993 + -1.0 541,010 + -0.3 541,011 - -1.6 541,012 + -0.1 541,014 + -0.2 541,014 + +0.0 541,015 - +1.4 541,136 - HMPREF1058_RS06180 0.14 -0.4 541,136 - HMPREF1058_RS06180 0.14 -2.2 541,136 - HMPREF1058_RS06180 0.14 -2.4 541,136 - HMPREF1058_RS06180 0.14 -1.4 541,149 - HMPREF1058_RS06180 0.16 +1.6 541,169 + HMPREF1058_RS06180 0.19 -0.5 541,328 - HMPREF1058_RS06180 0.45 -3.2 541,377 + HMPREF1058_RS06180 0.53 +1.2 541,377 + HMPREF1058_RS06180 0.53 -1.3 541,378 - HMPREF1058_RS06180 0.53 -0.5 541,396 + HMPREF1058_RS06180 0.56 +1.8 541,396 + HMPREF1058_RS06180 0.56 -0.8 541,397 - HMPREF1058_RS06180 0.57 -1.2 541,414 + HMPREF1058_RS06180 0.59 +0.7 541,415 - HMPREF1058_RS06180 0.59 -0.5 541,615 + -0.8 541,616 - -3.2 541,683 + -1.8 541,684 - -0.0 541,697 + -0.7 541,697 + +0.2 541,698 - -0.2 541,828 + -4.2 541,831 - -0.5 541,842 - -1.4 542,129 - HMPREF1058_RS06175 0.40 -1.4 542,335 - HMPREF1058_RS06175 0.67 -0.9 542,336 + HMPREF1058_RS06175 0.67 +0.9 542,336 + HMPREF1058_RS06175 0.67 +2.4 542,337 - HMPREF1058_RS06175 0.67 -1.2 542,337 - HMPREF1058_RS06175 0.67 +2.0 542,467 + HMPREF1058_RS06175 0.85 -3.1 542,471 + HMPREF1058_RS06175 0.85 -2.0 542,471 + HMPREF1058_RS06175 0.85 +0.1 542,529 + -1.0 542,594 - +1.4 542,594 - +0.4 542,635 + -0.2
Or see this region's nucleotide sequence