Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS05135 and HMPREF1058_RS21645 are separated by 470 nucleotides HMPREF1058_RS21645 and HMPREF1058_RS05130 are separated by 259 nucleotides HMPREF1058_RS05130 and HMPREF1058_RS05125 are separated by 17 nucleotides HMPREF1058_RS05125 and HMPREF1058_RS05120 are separated by 510 nucleotides
HMPREF1058_RS05135: HMPREF1058_RS05135 - arylsulfatase, at 237,262 to 238,779
_RS05135
HMPREF1058_RS21645: HMPREF1058_RS21645 - hypothetical protein, at 239,250 to 239,498
_RS21645
HMPREF1058_RS05130: HMPREF1058_RS05130 - site-specific integrase, at 239,758 to 240,990
_RS05130
HMPREF1058_RS05125: HMPREF1058_RS05125 - hypothetical protein, at 241,008 to 241,400
_RS05125
HMPREF1058_RS05120: HMPREF1058_RS05120 - plasmid recombination protein, at 241,911 to 242,722
_RS05120
Position (kb) 239
240
241 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 238.839 kb on - strand at 238.910 kb on + strand at 238.911 kb on - strand at 238.916 kb on - strand at 239.057 kb on + strand at 239.214 kb on + strand at 239.214 kb on + strand at 239.352 kb on + strand, within HMPREF1058_RS21645 at 239.352 kb on + strand, within HMPREF1058_RS21645 at 239.353 kb on - strand, within HMPREF1058_RS21645 at 239.353 kb on - strand, within HMPREF1058_RS21645 at 239.353 kb on - strand, within HMPREF1058_RS21645 at 239.353 kb on - strand, within HMPREF1058_RS21645 at 239.353 kb on - strand, within HMPREF1058_RS21645 at 239.373 kb on - strand, within HMPREF1058_RS21645 at 239.488 kb on - strand at 239.591 kb on + strand at 239.766 kb on + strand at 239.778 kb on - strand at 239.780 kb on + strand at 239.967 kb on + strand, within HMPREF1058_RS05130 at 239.968 kb on - strand, within HMPREF1058_RS05130 at 240.022 kb on + strand, within HMPREF1058_RS05130 at 240.023 kb on - strand, within HMPREF1058_RS05130 at 240.319 kb on - strand, within HMPREF1058_RS05130 at 240.491 kb on + strand, within HMPREF1058_RS05130 at 240.660 kb on + strand, within HMPREF1058_RS05130 at 240.673 kb on + strand, within HMPREF1058_RS05130 at 240.737 kb on + strand, within HMPREF1058_RS05130 at 240.787 kb on + strand, within HMPREF1058_RS05130 at 240.788 kb on - strand, within HMPREF1058_RS05130 at 240.879 kb on - strand at 240.921 kb on - strand at 240.973 kb on - strand at 240.989 kb on - strand at 240.993 kb on - strand at 241.072 kb on + strand, within HMPREF1058_RS05125 at 241.073 kb on - strand, within HMPREF1058_RS05125 at 241.180 kb on + strand, within HMPREF1058_RS05125 at 241.180 kb on + strand, within HMPREF1058_RS05125 at 241.180 kb on + strand, within HMPREF1058_RS05125 at 241.181 kb on - strand, within HMPREF1058_RS05125 at 241.181 kb on - strand, within HMPREF1058_RS05125 at 241.181 kb on - strand, within HMPREF1058_RS05125 at 241.183 kb on - strand, within HMPREF1058_RS05125 at 241.189 kb on + strand, within HMPREF1058_RS05125 at 241.189 kb on + strand, within HMPREF1058_RS05125 at 241.218 kb on + strand, within HMPREF1058_RS05125 at 241.321 kb on + strand, within HMPREF1058_RS05125 at 241.322 kb on - strand, within HMPREF1058_RS05125 at 241.406 kb on + strand at 241.423 kb on - strand at 241.423 kb on - strand at 241.436 kb on + strand at 241.437 kb on - strand at 241.438 kb on + strand at 241.439 kb on - strand at 241.440 kb on + strand at 241.440 kb on + strand at 241.440 kb on + strand at 241.440 kb on + strand at 241.441 kb on - strand at 241.441 kb on - strand at 241.441 kb on - strand at 241.441 kb on - strand at 241.452 kb on + strand at 241.453 kb on - strand at 241.590 kb on - strand at 241.728 kb on + strand at 241.774 kb on + strand at 241.778 kb on - strand at 241.778 kb on - strand at 241.781 kb on + strand at 241.866 kb on - strand at 241.880 kb on + strand at 241.880 kb on + strand at 241.880 kb on + strand at 241.881 kb on - strand at 241.927 kb on + strand at 241.973 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 238,839 - -2.0 238,910 + -0.8 238,911 - -2.7 238,916 - -1.1 239,057 + +1.8 239,214 + -0.2 239,214 + +1.0 239,352 + HMPREF1058_RS21645 0.41 -1.6 239,352 + HMPREF1058_RS21645 0.41 +0.6 239,353 - HMPREF1058_RS21645 0.41 -1.1 239,353 - HMPREF1058_RS21645 0.41 -0.8 239,353 - HMPREF1058_RS21645 0.41 -1.0 239,353 - HMPREF1058_RS21645 0.41 +0.5 239,353 - HMPREF1058_RS21645 0.41 -0.3 239,373 - HMPREF1058_RS21645 0.49 -0.2 239,488 - -1.5 239,591 + +0.4 239,766 + +0.3 239,778 - +0.0 239,780 + +0.6 239,967 + HMPREF1058_RS05130 0.17 +0.8 239,968 - HMPREF1058_RS05130 0.17 -0.7 240,022 + HMPREF1058_RS05130 0.21 +0.0 240,023 - HMPREF1058_RS05130 0.21 -0.3 240,319 - HMPREF1058_RS05130 0.45 -1.8 240,491 + HMPREF1058_RS05130 0.59 -0.6 240,660 + HMPREF1058_RS05130 0.73 +1.0 240,673 + HMPREF1058_RS05130 0.74 -2.2 240,737 + HMPREF1058_RS05130 0.79 -1.4 240,787 + HMPREF1058_RS05130 0.83 +2.1 240,788 - HMPREF1058_RS05130 0.84 -2.8 240,879 - +1.5 240,921 - -2.2 240,973 - -0.7 240,989 - -1.5 240,993 - +2.1 241,072 + HMPREF1058_RS05125 0.16 -2.5 241,073 - HMPREF1058_RS05125 0.17 +0.4 241,180 + HMPREF1058_RS05125 0.44 +1.1 241,180 + HMPREF1058_RS05125 0.44 +2.1 241,180 + HMPREF1058_RS05125 0.44 +0.3 241,181 - HMPREF1058_RS05125 0.44 -0.2 241,181 - HMPREF1058_RS05125 0.44 +1.0 241,181 - HMPREF1058_RS05125 0.44 -1.3 241,183 - HMPREF1058_RS05125 0.45 +2.4 241,189 + HMPREF1058_RS05125 0.46 +1.3 241,189 + HMPREF1058_RS05125 0.46 +0.0 241,218 + HMPREF1058_RS05125 0.53 +0.2 241,321 + HMPREF1058_RS05125 0.80 +0.9 241,322 - HMPREF1058_RS05125 0.80 -1.0 241,406 + +0.6 241,423 - +0.9 241,423 - +0.2 241,436 + -1.8 241,437 - +0.3 241,438 + +0.1 241,439 - +0.9 241,440 + +2.1 241,440 + -0.8 241,440 + +0.3 241,440 + -0.7 241,441 - +0.2 241,441 - -0.2 241,441 - -0.1 241,441 - +2.4 241,452 + -2.0 241,453 - +1.3 241,590 - -2.0 241,728 + +0.1 241,774 + -1.9 241,778 - +2.0 241,778 - -0.4 241,781 + +0.0 241,866 - -0.8 241,880 + +1.8 241,880 + +2.3 241,880 + -0.7 241,881 - +0.4 241,927 + -0.1 241,973 + -0.4
Or see this region's nucleotide sequence