Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS04385 and HMPREF1058_RS04380 are separated by 93 nucleotides HMPREF1058_RS04380 and HMPREF1058_RS04375 are separated by 114 nucleotides HMPREF1058_RS04375 and HMPREF1058_RS04370 are separated by 11 nucleotides
HMPREF1058_RS04385: HMPREF1058_RS04385 - methylated-DNA--[protein]-cysteine S-methyltransferase, at 47,647 to 48,174
_RS04385
HMPREF1058_RS04380: HMPREF1058_RS04380 - DUF4251 domain-containing protein, at 48,268 to 48,858
_RS04380
HMPREF1058_RS04375: HMPREF1058_RS04375 - carbon-nitrogen hydrolase, at 48,973 to 49,860
_RS04375
HMPREF1058_RS04370: HMPREF1058_RS04370 - agmatine deiminase family protein, at 49,872 to 50,903
_RS04370
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 47.975 kb on - strand, within HMPREF1058_RS04385 at 48.170 kb on + strand at 48.170 kb on + strand at 48.199 kb on + strand at 48.200 kb on - strand at 48.202 kb on - strand at 48.207 kb on + strand at 48.254 kb on - strand at 48.261 kb on + strand at 48.270 kb on - strand at 48.290 kb on - strand at 48.294 kb on + strand at 48.294 kb on + strand at 48.336 kb on + strand, within HMPREF1058_RS04380 at 48.359 kb on - strand, within HMPREF1058_RS04380 at 48.361 kb on - strand, within HMPREF1058_RS04380 at 48.361 kb on - strand, within HMPREF1058_RS04380 at 48.361 kb on - strand, within HMPREF1058_RS04380 at 48.364 kb on - strand, within HMPREF1058_RS04380 at 48.441 kb on + strand, within HMPREF1058_RS04380 at 48.442 kb on - strand, within HMPREF1058_RS04380 at 48.443 kb on + strand, within HMPREF1058_RS04380 at 48.479 kb on - strand, within HMPREF1058_RS04380 at 48.479 kb on - strand, within HMPREF1058_RS04380 at 48.535 kb on - strand, within HMPREF1058_RS04380 at 48.594 kb on - strand, within HMPREF1058_RS04380 at 48.638 kb on - strand, within HMPREF1058_RS04380 at 48.638 kb on - strand, within HMPREF1058_RS04380 at 48.677 kb on - strand, within HMPREF1058_RS04380 at 48.685 kb on - strand, within HMPREF1058_RS04380 at 48.685 kb on - strand, within HMPREF1058_RS04380 at 48.699 kb on - strand, within HMPREF1058_RS04380 at 48.700 kb on - strand, within HMPREF1058_RS04380 at 48.827 kb on - strand at 48.874 kb on + strand at 48.885 kb on + strand at 48.886 kb on - strand at 48.909 kb on - strand at 48.970 kb on + strand at 49.086 kb on + strand, within HMPREF1058_RS04375 at 49.121 kb on - strand, within HMPREF1058_RS04375 at 49.276 kb on + strand, within HMPREF1058_RS04375 at 49.322 kb on - strand, within HMPREF1058_RS04375 at 49.322 kb on - strand, within HMPREF1058_RS04375 at 49.324 kb on - strand, within HMPREF1058_RS04375 at 49.330 kb on - strand, within HMPREF1058_RS04375 at 49.335 kb on - strand, within HMPREF1058_RS04375 at 49.335 kb on - strand, within HMPREF1058_RS04375 at 49.337 kb on - strand, within HMPREF1058_RS04375 at 49.337 kb on - strand, within HMPREF1058_RS04375 at 49.337 kb on - strand, within HMPREF1058_RS04375 at 49.338 kb on + strand, within HMPREF1058_RS04375 at 49.408 kb on + strand, within HMPREF1058_RS04375 at 49.408 kb on + strand, within HMPREF1058_RS04375 at 49.409 kb on - strand, within HMPREF1058_RS04375 at 49.503 kb on + strand, within HMPREF1058_RS04375 at 49.528 kb on + strand, within HMPREF1058_RS04375 at 49.610 kb on - strand, within HMPREF1058_RS04375 at 49.775 kb on - strand at 49.833 kb on + strand at 49.834 kb on - strand at 49.860 kb on + strand at 49.864 kb on + strand at 49.865 kb on - strand at 49.866 kb on + strand at 49.876 kb on + strand at 49.876 kb on + strand at 49.877 kb on - strand at 49.878 kb on + strand at 49.878 kb on + strand at 49.953 kb on + strand at 49.954 kb on - strand at 49.956 kb on + strand at 49.956 kb on + strand at 50.004 kb on - strand, within HMPREF1058_RS04370 at 50.023 kb on + strand, within HMPREF1058_RS04370 at 50.068 kb on + strand, within HMPREF1058_RS04370 at 50.101 kb on + strand, within HMPREF1058_RS04370 at 50.169 kb on + strand, within HMPREF1058_RS04370 at 50.170 kb on - strand, within HMPREF1058_RS04370 at 50.233 kb on + strand, within HMPREF1058_RS04370 at 50.234 kb on - strand, within HMPREF1058_RS04370 at 50.234 kb on - strand, within HMPREF1058_RS04370 at 50.322 kb on + strand, within HMPREF1058_RS04370 at 50.322 kb on + strand, within HMPREF1058_RS04370 at 50.334 kb on + strand, within HMPREF1058_RS04370 at 50.545 kb on + strand, within HMPREF1058_RS04370 at 50.701 kb on + strand, within HMPREF1058_RS04370 at 50.701 kb on + strand, within HMPREF1058_RS04370 at 50.709 kb on + strand, within HMPREF1058_RS04370 at 50.709 kb on + strand, within HMPREF1058_RS04370 at 50.714 kb on + strand, within HMPREF1058_RS04370 at 50.714 kb on + strand, within HMPREF1058_RS04370 at 50.714 kb on + strand, within HMPREF1058_RS04370 at 50.715 kb on - strand, within HMPREF1058_RS04370 at 50.715 kb on - strand, within HMPREF1058_RS04370 at 50.818 kb on + strand at 50.818 kb on + strand at 50.819 kb on - strand at 50.851 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 47,975 - HMPREF1058_RS04385 0.62 +0.5 48,170 + +0.5 48,170 + -1.8 48,199 + -0.0 48,200 - +1.0 48,202 - +0.4 48,207 + +2.2 48,254 - -0.6 48,261 + -0.6 48,270 - +1.8 48,290 - +0.2 48,294 + +0.9 48,294 + -0.8 48,336 + HMPREF1058_RS04380 0.12 +0.2 48,359 - HMPREF1058_RS04380 0.15 -0.2 48,361 - HMPREF1058_RS04380 0.16 -1.8 48,361 - HMPREF1058_RS04380 0.16 +0.2 48,361 - HMPREF1058_RS04380 0.16 -0.5 48,364 - HMPREF1058_RS04380 0.16 -1.2 48,441 + HMPREF1058_RS04380 0.29 +1.7 48,442 - HMPREF1058_RS04380 0.29 +0.4 48,443 + HMPREF1058_RS04380 0.30 -0.3 48,479 - HMPREF1058_RS04380 0.36 -1.5 48,479 - HMPREF1058_RS04380 0.36 -0.6 48,535 - HMPREF1058_RS04380 0.45 +2.0 48,594 - HMPREF1058_RS04380 0.55 -0.8 48,638 - HMPREF1058_RS04380 0.63 -0.3 48,638 - HMPREF1058_RS04380 0.63 -1.0 48,677 - HMPREF1058_RS04380 0.69 -0.4 48,685 - HMPREF1058_RS04380 0.71 +0.7 48,685 - HMPREF1058_RS04380 0.71 +0.2 48,699 - HMPREF1058_RS04380 0.73 +0.3 48,700 - HMPREF1058_RS04380 0.73 +0.3 48,827 - -0.9 48,874 + -0.5 48,885 + +1.3 48,886 - +0.1 48,909 - -1.6 48,970 + -0.0 49,086 + HMPREF1058_RS04375 0.13 -1.2 49,121 - HMPREF1058_RS04375 0.17 -1.5 49,276 + HMPREF1058_RS04375 0.34 +0.1 49,322 - HMPREF1058_RS04375 0.39 -1.0 49,322 - HMPREF1058_RS04375 0.39 -1.2 49,324 - HMPREF1058_RS04375 0.40 -1.9 49,330 - HMPREF1058_RS04375 0.40 -2.9 49,335 - HMPREF1058_RS04375 0.41 +0.3 49,335 - HMPREF1058_RS04375 0.41 +1.2 49,337 - HMPREF1058_RS04375 0.41 -2.0 49,337 - HMPREF1058_RS04375 0.41 -1.9 49,337 - HMPREF1058_RS04375 0.41 -0.5 49,338 + HMPREF1058_RS04375 0.41 -1.1 49,408 + HMPREF1058_RS04375 0.49 +1.1 49,408 + HMPREF1058_RS04375 0.49 +0.3 49,409 - HMPREF1058_RS04375 0.49 -2.4 49,503 + HMPREF1058_RS04375 0.60 -0.6 49,528 + HMPREF1058_RS04375 0.62 -2.9 49,610 - HMPREF1058_RS04375 0.72 -1.6 49,775 - -1.4 49,833 + -0.4 49,834 - +1.4 49,860 + -0.8 49,864 + -1.0 49,865 - -1.5 49,866 + +1.2 49,876 + -2.4 49,876 + -0.2 49,877 - -0.1 49,878 + -0.2 49,878 + +0.7 49,953 + +0.4 49,954 - +3.0 49,956 + +0.7 49,956 + +0.9 50,004 - HMPREF1058_RS04370 0.13 +2.1 50,023 + HMPREF1058_RS04370 0.15 +0.2 50,068 + HMPREF1058_RS04370 0.19 -0.7 50,101 + HMPREF1058_RS04370 0.22 +2.5 50,169 + HMPREF1058_RS04370 0.29 -0.4 50,170 - HMPREF1058_RS04370 0.29 +0.9 50,233 + HMPREF1058_RS04370 0.35 +2.9 50,234 - HMPREF1058_RS04370 0.35 -0.9 50,234 - HMPREF1058_RS04370 0.35 +1.0 50,322 + HMPREF1058_RS04370 0.44 +2.7 50,322 + HMPREF1058_RS04370 0.44 +1.1 50,334 + HMPREF1058_RS04370 0.45 +3.6 50,545 + HMPREF1058_RS04370 0.65 -0.2 50,701 + HMPREF1058_RS04370 0.80 +1.3 50,701 + HMPREF1058_RS04370 0.80 +0.3 50,709 + HMPREF1058_RS04370 0.81 +1.2 50,709 + HMPREF1058_RS04370 0.81 +1.9 50,714 + HMPREF1058_RS04370 0.82 +1.7 50,714 + HMPREF1058_RS04370 0.82 +0.8 50,714 + HMPREF1058_RS04370 0.82 +2.2 50,715 - HMPREF1058_RS04370 0.82 +1.7 50,715 - HMPREF1058_RS04370 0.82 -0.2 50,818 + +1.4 50,818 + -0.2 50,819 - -0.3 50,851 - +0.5
Or see this region's nucleotide sequence