Experiment: Lacto-N-tetraose 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS03300 and HMPREF1058_RS03295 are separated by 385 nucleotides HMPREF1058_RS03295 and HMPREF1058_RS03290 overlap by 11 nucleotides HMPREF1058_RS03290 and HMPREF1058_RS03285 are separated by 17 nucleotides HMPREF1058_RS03285 and HMPREF1058_RS03280 are separated by 8 nucleotides
HMPREF1058_RS03300: HMPREF1058_RS03300 - superoxide dismutase, at 312,621 to 313,238
_RS03300
HMPREF1058_RS03295: HMPREF1058_RS03295 - sulfur carrier protein ThiS, at 313,624 to 313,824
_RS03295
HMPREF1058_RS03290: HMPREF1058_RS03290 - thiamine phosphate synthase, at 313,814 to 314,440
_RS03290
HMPREF1058_RS03285: HMPREF1058_RS03285 - thiazole synthase, at 314,458 to 315,231
_RS03285
HMPREF1058_RS03280: HMPREF1058_RS03280 - phosphomethylpyrimidine synthase ThiC, at 315,240 to 316,940
_RS03280
Position (kb) 313
314
315 Strain fitness (log2 ratio) -2
-1
0
1
2 at 312.850 kb on + strand, within HMPREF1058_RS03300 at 312.882 kb on - strand, within HMPREF1058_RS03300 at 312.886 kb on + strand, within HMPREF1058_RS03300 at 313.063 kb on + strand, within HMPREF1058_RS03300 at 313.063 kb on + strand, within HMPREF1058_RS03300 at 313.064 kb on - strand, within HMPREF1058_RS03300 at 313.064 kb on - strand, within HMPREF1058_RS03300 at 313.068 kb on - strand, within HMPREF1058_RS03300 at 313.068 kb on - strand, within HMPREF1058_RS03300 at 313.135 kb on + strand at 313.136 kb on - strand, within HMPREF1058_RS03300 at 313.149 kb on + strand, within HMPREF1058_RS03300 at 313.149 kb on + strand, within HMPREF1058_RS03300 at 313.150 kb on - strand, within HMPREF1058_RS03300 at 313.185 kb on - strand at 313.219 kb on + strand at 313.219 kb on + strand at 313.220 kb on - strand at 313.235 kb on - strand at 313.235 kb on - strand at 313.237 kb on - strand at 313.252 kb on + strand at 313.253 kb on - strand at 313.254 kb on + strand at 313.254 kb on + strand at 313.254 kb on + strand at 313.254 kb on + strand at 313.261 kb on + strand at 313.261 kb on + strand at 313.262 kb on - strand at 313.325 kb on + strand at 313.325 kb on + strand at 313.335 kb on - strand at 313.335 kb on - strand at 313.358 kb on + strand at 313.359 kb on - strand at 313.378 kb on + strand at 313.409 kb on + strand at 313.425 kb on - strand at 313.461 kb on + strand at 313.462 kb on - strand at 313.505 kb on + strand at 313.507 kb on + strand at 313.507 kb on + strand at 313.550 kb on + strand at 313.618 kb on + strand at 313.619 kb on - strand at 313.620 kb on + strand at 313.621 kb on - strand at 313.621 kb on - strand at 313.623 kb on + strand at 313.659 kb on - strand, within HMPREF1058_RS03295 at 313.754 kb on + strand, within HMPREF1058_RS03295 at 313.865 kb on + strand at 313.866 kb on - strand at 313.866 kb on - strand at 313.866 kb on - strand at 313.871 kb on + strand at 313.875 kb on - strand at 313.924 kb on - strand, within HMPREF1058_RS03290 at 314.106 kb on + strand, within HMPREF1058_RS03290 at 314.107 kb on - strand, within HMPREF1058_RS03290 at 314.155 kb on - strand, within HMPREF1058_RS03290 at 314.183 kb on + strand, within HMPREF1058_RS03290 at 314.184 kb on - strand, within HMPREF1058_RS03290 at 314.388 kb on + strand at 314.389 kb on - strand at 314.441 kb on + strand at 314.455 kb on - strand at 314.455 kb on - strand at 314.475 kb on - strand at 314.490 kb on + strand at 314.623 kb on - strand, within HMPREF1058_RS03285 at 314.634 kb on + strand, within HMPREF1058_RS03285 at 314.651 kb on + strand, within HMPREF1058_RS03285 at 314.652 kb on - strand, within HMPREF1058_RS03285 at 314.652 kb on - strand, within HMPREF1058_RS03285 at 314.657 kb on + strand at 314.668 kb on - strand, within HMPREF1058_RS03285 at 314.829 kb on - strand, within HMPREF1058_RS03285 at 314.865 kb on + strand, within HMPREF1058_RS03285 at 314.865 kb on + strand, within HMPREF1058_RS03285 at 314.866 kb on - strand, within HMPREF1058_RS03285 at 314.866 kb on - strand, within HMPREF1058_RS03285 at 315.000 kb on - strand, within HMPREF1058_RS03285 at 315.003 kb on - strand, within HMPREF1058_RS03285 at 315.075 kb on + strand, within HMPREF1058_RS03285 at 315.117 kb on - strand, within HMPREF1058_RS03285 at 315.168 kb on - strand at 315.224 kb on + strand at 315.239 kb on + strand at 315.293 kb on + strand at 315.294 kb on - strand at 315.316 kb on - strand at 315.326 kb on - strand at 315.364 kb on + strand at 315.407 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Lacto-N-tetraose 10 mg/mL (C) remove 312,850 + HMPREF1058_RS03300 0.37 +0.7 312,882 - HMPREF1058_RS03300 0.42 -0.7 312,886 + HMPREF1058_RS03300 0.43 -0.5 313,063 + HMPREF1058_RS03300 0.72 -0.8 313,063 + HMPREF1058_RS03300 0.72 -1.4 313,064 - HMPREF1058_RS03300 0.72 +0.2 313,064 - HMPREF1058_RS03300 0.72 +1.4 313,068 - HMPREF1058_RS03300 0.72 +1.2 313,068 - HMPREF1058_RS03300 0.72 +0.9 313,135 + +0.5 313,136 - HMPREF1058_RS03300 0.83 +1.7 313,149 + HMPREF1058_RS03300 0.85 -1.1 313,149 + HMPREF1058_RS03300 0.85 -0.9 313,150 - HMPREF1058_RS03300 0.86 +0.9 313,185 - -2.0 313,219 + -0.2 313,219 + -1.1 313,220 - -0.5 313,235 - +2.1 313,235 - +0.4 313,237 - +1.2 313,252 + +1.6 313,253 - +1.0 313,254 + -0.2 313,254 + -1.8 313,254 + -0.6 313,254 + +0.4 313,261 + +1.6 313,261 + +1.3 313,262 - -1.5 313,325 + -1.8 313,325 + +0.6 313,335 - +0.8 313,335 - +0.2 313,358 + -0.6 313,359 - +1.5 313,378 + +0.7 313,409 + -0.6 313,425 - -0.3 313,461 + -0.2 313,462 - -0.8 313,505 + -1.3 313,507 + +1.7 313,507 + +1.7 313,550 + -0.2 313,618 + -0.8 313,619 - -0.3 313,620 + +0.9 313,621 - -1.4 313,621 - +0.9 313,623 + +0.4 313,659 - HMPREF1058_RS03295 0.17 +0.0 313,754 + HMPREF1058_RS03295 0.65 -1.5 313,865 + -0.1 313,866 - +0.4 313,866 - +0.5 313,866 - -0.7 313,871 + -0.4 313,875 - +0.5 313,924 - HMPREF1058_RS03290 0.18 +0.8 314,106 + HMPREF1058_RS03290 0.47 -0.4 314,107 - HMPREF1058_RS03290 0.47 -1.0 314,155 - HMPREF1058_RS03290 0.54 -1.2 314,183 + HMPREF1058_RS03290 0.59 -0.5 314,184 - HMPREF1058_RS03290 0.59 -0.8 314,388 + +1.2 314,389 - -1.8 314,441 + +0.2 314,455 - -0.5 314,455 - -2.0 314,475 - -0.5 314,490 + -0.2 314,623 - HMPREF1058_RS03285 0.21 +1.7 314,634 + HMPREF1058_RS03285 0.23 -0.3 314,651 + HMPREF1058_RS03285 0.25 -0.8 314,652 - HMPREF1058_RS03285 0.25 +0.1 314,652 - HMPREF1058_RS03285 0.25 -0.2 314,657 + +1.4 314,668 - HMPREF1058_RS03285 0.27 +0.4 314,829 - HMPREF1058_RS03285 0.48 -1.0 314,865 + HMPREF1058_RS03285 0.53 +1.3 314,865 + HMPREF1058_RS03285 0.53 +0.0 314,866 - HMPREF1058_RS03285 0.53 +0.5 314,866 - HMPREF1058_RS03285 0.53 -0.5 315,000 - HMPREF1058_RS03285 0.70 +1.2 315,003 - HMPREF1058_RS03285 0.70 -0.4 315,075 + HMPREF1058_RS03285 0.80 -1.2 315,117 - HMPREF1058_RS03285 0.85 -1.6 315,168 - -0.5 315,224 + -0.7 315,239 + +0.6 315,293 + +0.2 315,294 - +0.5 315,316 - -1.5 315,326 - -0.4 315,364 + -1.6 315,407 + -1.3
Or see this region's nucleotide sequence