Experiment: ISOT Umich-01 10 mg/mL (C)
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS07450 and HMPREF1058_RS07445 are separated by 89 nucleotides HMPREF1058_RS07445 and HMPREF1058_RS07440 are separated by 117 nucleotides HMPREF1058_RS07440 and HMPREF1058_RS07435 are separated by 202 nucleotides HMPREF1058_RS07435 and HMPREF1058_RS07430 are separated by 311 nucleotides
HMPREF1058_RS07450: HMPREF1058_RS07450 - LPS-assembly protein LptD, at 220,960 to 223,695
_RS07450
HMPREF1058_RS07445: HMPREF1058_RS07445 - uracil-DNA glycosylase, at 223,785 to 224,447
_RS07445
HMPREF1058_RS07440: HMPREF1058_RS07440 - aspartate--ammonia ligase, at 224,565 to 225,605
_RS07440
HMPREF1058_RS07435: HMPREF1058_RS07435 - tRNA-Arg, at 225,808 to 225,879
_RS07435
HMPREF1058_RS07430: HMPREF1058_RS07430 - TonB-dependent receptor, at 226,191 to 229,466
_RS07430
Position (kb)
224
225
226 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 223.694 kb on - strand at 223.780 kb on + strand at 223.781 kb on - strand at 223.867 kb on + strand, within HMPREF1058_RS07445 at 223.867 kb on + strand, within HMPREF1058_RS07445 at 223.968 kb on + strand, within HMPREF1058_RS07445 at 223.969 kb on - strand, within HMPREF1058_RS07445 at 223.974 kb on - strand, within HMPREF1058_RS07445 at 224.016 kb on - strand, within HMPREF1058_RS07445 at 224.088 kb on + strand, within HMPREF1058_RS07445 at 224.088 kb on + strand, within HMPREF1058_RS07445 at 224.172 kb on - strand, within HMPREF1058_RS07445 at 224.316 kb on - strand, within HMPREF1058_RS07445 at 224.336 kb on + strand, within HMPREF1058_RS07445 at 224.361 kb on + strand, within HMPREF1058_RS07445 at 224.433 kb on + strand at 224.434 kb on - strand at 224.460 kb on - strand at 224.509 kb on - strand at 224.560 kb on + strand at 224.563 kb on - strand at 224.621 kb on + strand at 224.640 kb on + strand at 224.686 kb on - strand, within HMPREF1058_RS07440 at 224.686 kb on - strand, within HMPREF1058_RS07440 at 224.860 kb on - strand, within HMPREF1058_RS07440 at 224.910 kb on - strand at 224.972 kb on + strand, within HMPREF1058_RS07440 at 224.977 kb on - strand, within HMPREF1058_RS07440 at 224.977 kb on - strand, within HMPREF1058_RS07440 at 224.981 kb on + strand, within HMPREF1058_RS07440 at 224.982 kb on - strand, within HMPREF1058_RS07440 at 224.985 kb on + strand, within HMPREF1058_RS07440 at 225.044 kb on - strand, within HMPREF1058_RS07440 at 225.044 kb on - strand at 225.123 kb on + strand, within HMPREF1058_RS07440 at 225.128 kb on + strand, within HMPREF1058_RS07440 at 225.129 kb on - strand, within HMPREF1058_RS07440 at 225.159 kb on - strand, within HMPREF1058_RS07440 at 225.168 kb on + strand, within HMPREF1058_RS07440 at 225.168 kb on + strand, within HMPREF1058_RS07440 at 225.169 kb on - strand, within HMPREF1058_RS07440 at 225.234 kb on + strand, within HMPREF1058_RS07440 at 225.239 kb on - strand, within HMPREF1058_RS07440 at 225.239 kb on - strand, within HMPREF1058_RS07440 at 225.239 kb on - strand, within HMPREF1058_RS07440 at 225.240 kb on + strand, within HMPREF1058_RS07440 at 225.240 kb on + strand, within HMPREF1058_RS07440 at 225.317 kb on + strand, within HMPREF1058_RS07440 at 225.322 kb on + strand, within HMPREF1058_RS07440 at 225.395 kb on + strand, within HMPREF1058_RS07440 at 225.493 kb on - strand at 225.577 kb on + strand at 225.620 kb on - strand at 225.704 kb on + strand at 225.928 kb on + strand at 225.930 kb on + strand at 226.042 kb on + strand at 226.062 kb on - strand at 226.083 kb on - strand at 226.216 kb on + strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.276 kb on + strand at 226.365 kb on - strand at 226.365 kb on - strand at 226.405 kb on - strand at 226.406 kb on + strand at 226.407 kb on - strand at 226.408 kb on + strand at 226.409 kb on - strand at 226.452 kb on + strand at 226.570 kb on + strand, within HMPREF1058_RS07430 at 226.573 kb on + strand, within HMPREF1058_RS07430 at 226.586 kb on + strand, within HMPREF1058_RS07430
Per-strain Table
Position Strand Gene LocusTag Fraction ISOT Umich-01 10 mg/mL (C) remove 223,694 - -0.4 223,780 + +0.3 223,781 - -0.4 223,867 + HMPREF1058_RS07445 0.12 +0.1 223,867 + HMPREF1058_RS07445 0.12 +1.1 223,968 + HMPREF1058_RS07445 0.28 -1.0 223,969 - HMPREF1058_RS07445 0.28 -0.2 223,974 - HMPREF1058_RS07445 0.29 +2.8 224,016 - HMPREF1058_RS07445 0.35 +0.9 224,088 + HMPREF1058_RS07445 0.46 +1.1 224,088 + HMPREF1058_RS07445 0.46 -0.5 224,172 - HMPREF1058_RS07445 0.58 +1.6 224,316 - HMPREF1058_RS07445 0.80 +0.6 224,336 + HMPREF1058_RS07445 0.83 -0.5 224,361 + HMPREF1058_RS07445 0.87 -0.7 224,433 + -0.1 224,434 - -0.3 224,460 - +0.5 224,509 - -0.0 224,560 + +1.6 224,563 - +0.5 224,621 + -1.6 224,640 + -0.8 224,686 - HMPREF1058_RS07440 0.12 -2.2 224,686 - HMPREF1058_RS07440 0.12 -2.9 224,860 - HMPREF1058_RS07440 0.28 -0.8 224,910 - -0.6 224,972 + HMPREF1058_RS07440 0.39 -2.4 224,977 - HMPREF1058_RS07440 0.40 -1.5 224,977 - HMPREF1058_RS07440 0.40 -4.6 224,981 + HMPREF1058_RS07440 0.40 -0.6 224,982 - HMPREF1058_RS07440 0.40 -3.5 224,985 + HMPREF1058_RS07440 0.40 -2.7 225,044 - HMPREF1058_RS07440 0.46 -0.5 225,044 - -1.3 225,123 + HMPREF1058_RS07440 0.54 -2.1 225,128 + HMPREF1058_RS07440 0.54 -0.3 225,129 - HMPREF1058_RS07440 0.54 +1.5 225,159 - HMPREF1058_RS07440 0.57 -3.5 225,168 + HMPREF1058_RS07440 0.58 -2.5 225,168 + HMPREF1058_RS07440 0.58 -0.3 225,169 - HMPREF1058_RS07440 0.58 -3.4 225,234 + HMPREF1058_RS07440 0.64 -1.5 225,239 - HMPREF1058_RS07440 0.65 -0.3 225,239 - HMPREF1058_RS07440 0.65 -3.0 225,239 - HMPREF1058_RS07440 0.65 -0.5 225,240 + HMPREF1058_RS07440 0.65 +2.5 225,240 + HMPREF1058_RS07440 0.65 -1.6 225,317 + HMPREF1058_RS07440 0.72 -1.2 225,322 + HMPREF1058_RS07440 0.73 -0.3 225,395 + HMPREF1058_RS07440 0.80 +0.5 225,493 - -0.6 225,577 + -1.4 225,620 - +0.1 225,704 + -0.9 225,928 + +1.1 225,930 + +0.1 226,042 + +2.0 226,062 - -0.9 226,083 - -2.3 226,216 + +0.4 226,276 + +0.5 226,276 + +1.1 226,276 + -0.6 226,365 - -0.0 226,365 - +0.0 226,405 - -0.1 226,406 + -1.5 226,407 - +0.0 226,408 + -1.7 226,409 - -0.6 226,452 + -2.2 226,570 + HMPREF1058_RS07430 0.12 +0.1 226,573 + HMPREF1058_RS07430 0.12 -0.6 226,586 + HMPREF1058_RS07430 0.12 -0.0
Or see this region's nucleotide sequence