Experiment: Brucella 1x (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS14595 and HMPREF1058_RS14590 are separated by 96 nucleotides HMPREF1058_RS14590 and HMPREF1058_RS14585 are separated by 331 nucleotides HMPREF1058_RS14585 and HMPREF1058_RS14580 are separated by 97 nucleotides
HMPREF1058_RS14595: HMPREF1058_RS14595 - dihydrofolate reductase, at 1,161,238 to 1,163,250
_RS14595
HMPREF1058_RS14590: HMPREF1058_RS14590 - AraC family transcriptional regulator, at 1,163,347 to 1,163,814
_RS14590
HMPREF1058_RS14585: HMPREF1058_RS14585 - DNA helicase RecQ, at 1,164,146 to 1,165,963
_RS14585
HMPREF1058_RS14580: HMPREF1058_RS14580 - tetratricopeptide repeat protein, at 1,166,061 to 1,166,915
_RS14580
Position (kb)
1164
1165
1166 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1163.187 kb on + strand at 1163.187 kb on + strand at 1163.221 kb on - strand at 1163.271 kb on + strand at 1163.325 kb on + strand at 1163.328 kb on - strand at 1163.371 kb on - strand at 1163.444 kb on - strand, within HMPREF1058_RS14590 at 1163.472 kb on + strand, within HMPREF1058_RS14590 at 1163.479 kb on - strand, within HMPREF1058_RS14590 at 1163.518 kb on - strand, within HMPREF1058_RS14590 at 1163.590 kb on - strand, within HMPREF1058_RS14590 at 1163.627 kb on + strand, within HMPREF1058_RS14590 at 1163.851 kb on - strand at 1163.855 kb on - strand at 1163.859 kb on - strand at 1163.869 kb on - strand at 1163.872 kb on + strand at 1163.991 kb on + strand at 1163.991 kb on + strand at 1163.992 kb on - strand at 1164.012 kb on + strand at 1164.123 kb on - strand at 1164.145 kb on + strand at 1164.146 kb on - strand at 1164.146 kb on - strand at 1164.205 kb on + strand at 1164.206 kb on - strand at 1164.217 kb on + strand at 1164.243 kb on + strand at 1164.308 kb on - strand at 1164.363 kb on + strand, within HMPREF1058_RS14585 at 1164.364 kb on - strand at 1164.491 kb on - strand, within HMPREF1058_RS14585 at 1164.592 kb on + strand, within HMPREF1058_RS14585 at 1164.609 kb on + strand, within HMPREF1058_RS14585 at 1164.610 kb on - strand, within HMPREF1058_RS14585 at 1164.640 kb on - strand, within HMPREF1058_RS14585 at 1164.717 kb on - strand, within HMPREF1058_RS14585 at 1164.840 kb on - strand, within HMPREF1058_RS14585 at 1165.018 kb on + strand, within HMPREF1058_RS14585 at 1165.029 kb on + strand, within HMPREF1058_RS14585 at 1165.049 kb on - strand, within HMPREF1058_RS14585 at 1165.077 kb on - strand, within HMPREF1058_RS14585 at 1165.133 kb on + strand, within HMPREF1058_RS14585 at 1165.196 kb on - strand, within HMPREF1058_RS14585 at 1165.227 kb on - strand, within HMPREF1058_RS14585 at 1165.269 kb on - strand, within HMPREF1058_RS14585 at 1165.283 kb on - strand, within HMPREF1058_RS14585 at 1165.385 kb on - strand, within HMPREF1058_RS14585 at 1165.411 kb on - strand, within HMPREF1058_RS14585 at 1165.413 kb on + strand, within HMPREF1058_RS14585 at 1165.414 kb on - strand, within HMPREF1058_RS14585 at 1165.419 kb on + strand, within HMPREF1058_RS14585 at 1165.495 kb on + strand, within HMPREF1058_RS14585 at 1165.496 kb on - strand, within HMPREF1058_RS14585 at 1165.562 kb on + strand, within HMPREF1058_RS14585 at 1165.563 kb on - strand, within HMPREF1058_RS14585 at 1165.609 kb on - strand, within HMPREF1058_RS14585 at 1165.762 kb on - strand, within HMPREF1058_RS14585 at 1165.798 kb on + strand at 1165.798 kb on + strand at 1165.808 kb on + strand at 1165.818 kb on + strand at 1165.859 kb on - strand at 1165.859 kb on - strand at 1165.867 kb on - strand at 1165.901 kb on + strand at 1165.903 kb on + strand at 1165.904 kb on - strand at 1165.932 kb on + strand at 1165.941 kb on + strand at 1165.951 kb on - strand at 1166.044 kb on + strand at 1166.137 kb on + strand at 1166.353 kb on + strand, within HMPREF1058_RS14580 at 1166.374 kb on - strand, within HMPREF1058_RS14580 at 1166.399 kb on + strand, within HMPREF1058_RS14580 at 1166.422 kb on - strand, within HMPREF1058_RS14580 at 1166.422 kb on - strand, within HMPREF1058_RS14580 at 1166.432 kb on + strand, within HMPREF1058_RS14580 at 1166.432 kb on + strand, within HMPREF1058_RS14580 at 1166.432 kb on + strand, within HMPREF1058_RS14580 at 1166.433 kb on - strand, within HMPREF1058_RS14580 at 1166.434 kb on + strand, within HMPREF1058_RS14580 at 1166.434 kb on + strand, within HMPREF1058_RS14580 at 1166.435 kb on - strand, within HMPREF1058_RS14580 at 1166.455 kb on + strand, within HMPREF1058_RS14580 at 1166.455 kb on + strand, within HMPREF1058_RS14580 at 1166.473 kb on + strand, within HMPREF1058_RS14580 at 1166.473 kb on + strand, within HMPREF1058_RS14580 at 1166.474 kb on - strand, within HMPREF1058_RS14580 at 1166.478 kb on - strand, within HMPREF1058_RS14580 at 1166.478 kb on - strand, within HMPREF1058_RS14580 at 1166.478 kb on - strand, within HMPREF1058_RS14580 at 1166.513 kb on + strand at 1166.546 kb on - strand, within HMPREF1058_RS14580 at 1166.576 kb on - strand, within HMPREF1058_RS14580 at 1166.649 kb on - strand, within HMPREF1058_RS14580 at 1166.697 kb on - strand, within HMPREF1058_RS14580 at 1166.711 kb on + strand, within HMPREF1058_RS14580 at 1166.819 kb on - strand, within HMPREF1058_RS14580 at 1166.840 kb on + strand at 1166.841 kb on - strand at 1166.841 kb on - strand at 1166.850 kb on + strand at 1166.859 kb on - strand at 1166.859 kb on - strand at 1166.868 kb on + strand at 1166.868 kb on + strand at 1166.868 kb on + strand at 1166.868 kb on + strand at 1166.869 kb on - strand at 1166.869 kb on - strand at 1166.869 kb on - strand at 1166.898 kb on - strand at 1166.920 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Brucella 1x (C) complex media remove 1,163,187 + -0.9 1,163,187 + -0.0 1,163,221 - -2.2 1,163,271 + -1.1 1,163,325 + -1.5 1,163,328 - -1.3 1,163,371 - -1.3 1,163,444 - HMPREF1058_RS14590 0.21 -1.1 1,163,472 + HMPREF1058_RS14590 0.27 -0.3 1,163,479 - HMPREF1058_RS14590 0.28 +2.3 1,163,518 - HMPREF1058_RS14590 0.37 -1.0 1,163,590 - HMPREF1058_RS14590 0.52 +0.7 1,163,627 + HMPREF1058_RS14590 0.60 -0.6 1,163,851 - -1.2 1,163,855 - +0.0 1,163,859 - -0.7 1,163,869 - +0.2 1,163,872 + +0.0 1,163,991 + -0.1 1,163,991 + +0.0 1,163,992 - +1.4 1,164,012 + -0.5 1,164,123 - -2.3 1,164,145 + +1.0 1,164,146 - -0.6 1,164,146 - -0.1 1,164,205 + +0.5 1,164,206 - -1.4 1,164,217 + -0.5 1,164,243 + -0.2 1,164,308 - -0.6 1,164,363 + HMPREF1058_RS14585 0.12 -1.2 1,164,364 - +0.7 1,164,491 - HMPREF1058_RS14585 0.19 -0.8 1,164,592 + HMPREF1058_RS14585 0.25 -1.1 1,164,609 + HMPREF1058_RS14585 0.25 +1.0 1,164,610 - HMPREF1058_RS14585 0.26 -1.0 1,164,640 - HMPREF1058_RS14585 0.27 -2.9 1,164,717 - HMPREF1058_RS14585 0.31 -1.1 1,164,840 - HMPREF1058_RS14585 0.38 -0.6 1,165,018 + HMPREF1058_RS14585 0.48 -1.7 1,165,029 + HMPREF1058_RS14585 0.49 -0.8 1,165,049 - HMPREF1058_RS14585 0.50 -1.6 1,165,077 - HMPREF1058_RS14585 0.51 -1.4 1,165,133 + HMPREF1058_RS14585 0.54 -1.4 1,165,196 - HMPREF1058_RS14585 0.58 -1.4 1,165,227 - HMPREF1058_RS14585 0.59 -0.1 1,165,269 - HMPREF1058_RS14585 0.62 +1.7 1,165,283 - HMPREF1058_RS14585 0.63 -0.5 1,165,385 - HMPREF1058_RS14585 0.68 +1.1 1,165,411 - HMPREF1058_RS14585 0.70 -0.7 1,165,413 + HMPREF1058_RS14585 0.70 -0.0 1,165,414 - HMPREF1058_RS14585 0.70 +0.3 1,165,419 + HMPREF1058_RS14585 0.70 -1.2 1,165,495 + HMPREF1058_RS14585 0.74 -2.1 1,165,496 - HMPREF1058_RS14585 0.74 -1.1 1,165,562 + HMPREF1058_RS14585 0.78 -0.9 1,165,563 - HMPREF1058_RS14585 0.78 -0.2 1,165,609 - HMPREF1058_RS14585 0.80 +1.7 1,165,762 - HMPREF1058_RS14585 0.89 -0.6 1,165,798 + +0.4 1,165,798 + -0.1 1,165,808 + -0.3 1,165,818 + -1.1 1,165,859 - +0.7 1,165,859 - +0.6 1,165,867 - +0.2 1,165,901 + +0.2 1,165,903 + -0.6 1,165,904 - -0.3 1,165,932 + -0.9 1,165,941 + +0.2 1,165,951 - -0.2 1,166,044 + +1.1 1,166,137 + -0.7 1,166,353 + HMPREF1058_RS14580 0.34 +0.7 1,166,374 - HMPREF1058_RS14580 0.37 -0.2 1,166,399 + HMPREF1058_RS14580 0.40 +1.1 1,166,422 - HMPREF1058_RS14580 0.42 +0.1 1,166,422 - HMPREF1058_RS14580 0.42 +0.9 1,166,432 + HMPREF1058_RS14580 0.43 -1.1 1,166,432 + HMPREF1058_RS14580 0.43 -0.4 1,166,432 + HMPREF1058_RS14580 0.43 +0.5 1,166,433 - HMPREF1058_RS14580 0.44 +0.5 1,166,434 + HMPREF1058_RS14580 0.44 -1.6 1,166,434 + HMPREF1058_RS14580 0.44 -0.8 1,166,435 - HMPREF1058_RS14580 0.44 -1.1 1,166,455 + HMPREF1058_RS14580 0.46 +1.4 1,166,455 + HMPREF1058_RS14580 0.46 -1.5 1,166,473 + HMPREF1058_RS14580 0.48 +0.7 1,166,473 + HMPREF1058_RS14580 0.48 -0.2 1,166,474 - HMPREF1058_RS14580 0.48 +0.1 1,166,478 - HMPREF1058_RS14580 0.49 -0.1 1,166,478 - HMPREF1058_RS14580 0.49 +0.6 1,166,478 - HMPREF1058_RS14580 0.49 +0.4 1,166,513 + +0.2 1,166,546 - HMPREF1058_RS14580 0.57 -0.8 1,166,576 - HMPREF1058_RS14580 0.60 +0.9 1,166,649 - HMPREF1058_RS14580 0.69 -0.2 1,166,697 - HMPREF1058_RS14580 0.74 -0.7 1,166,711 + HMPREF1058_RS14580 0.76 +1.3 1,166,819 - HMPREF1058_RS14580 0.89 -0.7 1,166,840 + -0.5 1,166,841 - -0.4 1,166,841 - -0.3 1,166,850 + -1.6 1,166,859 - -0.4 1,166,859 - +0.7 1,166,868 + -0.1 1,166,868 + -1.1 1,166,868 + -1.4 1,166,868 + +0.3 1,166,869 - +0.4 1,166,869 - -0.1 1,166,869 - -0.0 1,166,898 - +0.1 1,166,920 - -0.8
Or see this region's nucleotide sequence