Experiment: Brucella 1x (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS12250 and HMPREF1058_RS12245 are separated by 114 nucleotides HMPREF1058_RS12245 and HMPREF1058_RS12240 overlap by 4 nucleotides HMPREF1058_RS12240 and HMPREF1058_RS12235 are separated by 13 nucleotides
HMPREF1058_RS12250: HMPREF1058_RS12250 - RagB/SusD family nutrient uptake outer membrane protein, at 164,234 to 165,751
_RS12250
HMPREF1058_RS12245: HMPREF1058_RS12245 - LysO family transporter, at 165,866 to 166,147
_RS12245
HMPREF1058_RS12240: HMPREF1058_RS12240 - lysine exporter LysO family protein, at 166,144 to 166,746
_RS12240
HMPREF1058_RS12235: HMPREF1058_RS12235 - DUF4981 domain-containing protein, at 166,760 to 169,936
_RS12235
Position (kb) 166
167 Strain fitness (log2 ratio) -2
-1
0
1
2 at 165.244 kb on + strand, within HMPREF1058_RS12250 at 165.248 kb on + strand, within HMPREF1058_RS12250 at 165.248 kb on + strand, within HMPREF1058_RS12250 at 165.249 kb on - strand, within HMPREF1058_RS12250 at 165.249 kb on - strand, within HMPREF1058_RS12250 at 165.259 kb on - strand, within HMPREF1058_RS12250 at 165.280 kb on + strand, within HMPREF1058_RS12250 at 165.315 kb on - strand, within HMPREF1058_RS12250 at 165.316 kb on + strand, within HMPREF1058_RS12250 at 165.316 kb on + strand, within HMPREF1058_RS12250 at 165.435 kb on + strand, within HMPREF1058_RS12250 at 165.508 kb on - strand, within HMPREF1058_RS12250 at 165.611 kb on + strand at 165.704 kb on + strand at 165.749 kb on + strand at 165.773 kb on + strand at 165.774 kb on - strand at 165.776 kb on - strand at 165.776 kb on - strand at 165.779 kb on + strand at 165.780 kb on - strand at 165.819 kb on + strand at 165.835 kb on + strand at 165.847 kb on + strand at 165.852 kb on - strand at 165.861 kb on + strand at 165.862 kb on - strand at 165.864 kb on - strand at 165.865 kb on + strand at 165.866 kb on - strand at 165.866 kb on - strand at 165.976 kb on - strand, within HMPREF1058_RS12245 at 165.993 kb on + strand, within HMPREF1058_RS12245 at 165.994 kb on - strand, within HMPREF1058_RS12245 at 165.994 kb on - strand, within HMPREF1058_RS12245 at 166.036 kb on + strand, within HMPREF1058_RS12245 at 166.110 kb on - strand, within HMPREF1058_RS12245 at 166.110 kb on - strand, within HMPREF1058_RS12245 at 166.114 kb on - strand, within HMPREF1058_RS12245 at 166.114 kb on - strand, within HMPREF1058_RS12245 at 166.143 kb on + strand at 166.143 kb on + strand at 166.144 kb on - strand at 166.246 kb on + strand, within HMPREF1058_RS12240 at 166.429 kb on - strand, within HMPREF1058_RS12240 at 166.486 kb on + strand, within HMPREF1058_RS12240 at 166.486 kb on + strand, within HMPREF1058_RS12240 at 166.488 kb on + strand, within HMPREF1058_RS12240 at 166.712 kb on + strand at 166.741 kb on - strand at 166.741 kb on - strand at 166.777 kb on + strand at 166.787 kb on - strand at 166.893 kb on - strand at 167.045 kb on - strand at 167.080 kb on + strand, within HMPREF1058_RS12235 at 167.080 kb on - strand, within HMPREF1058_RS12235 at 167.126 kb on + strand, within HMPREF1058_RS12235 at 167.127 kb on - strand, within HMPREF1058_RS12235 at 167.137 kb on - strand, within HMPREF1058_RS12235 at 167.137 kb on - strand, within HMPREF1058_RS12235 at 167.198 kb on + strand, within HMPREF1058_RS12235 at 167.198 kb on + strand, within HMPREF1058_RS12235 at 167.199 kb on - strand, within HMPREF1058_RS12235 at 167.199 kb on - strand, within HMPREF1058_RS12235 at 167.199 kb on - strand, within HMPREF1058_RS12235 at 167.348 kb on - strand, within HMPREF1058_RS12235 at 167.414 kb on + strand, within HMPREF1058_RS12235 at 167.463 kb on + strand, within HMPREF1058_RS12235 at 167.463 kb on + strand, within HMPREF1058_RS12235 at 167.464 kb on - strand, within HMPREF1058_RS12235 at 167.464 kb on - strand, within HMPREF1058_RS12235 at 167.622 kb on + strand, within HMPREF1058_RS12235 at 167.647 kb on - strand, within HMPREF1058_RS12235
Per-strain Table
Position Strand Gene LocusTag Fraction Brucella 1x (C) complex media remove 165,244 + HMPREF1058_RS12250 0.67 +0.8 165,248 + HMPREF1058_RS12250 0.67 +0.2 165,248 + HMPREF1058_RS12250 0.67 -0.2 165,249 - HMPREF1058_RS12250 0.67 +2.7 165,249 - HMPREF1058_RS12250 0.67 +0.1 165,259 - HMPREF1058_RS12250 0.68 -1.8 165,280 + HMPREF1058_RS12250 0.69 +0.1 165,315 - HMPREF1058_RS12250 0.71 -0.9 165,316 + HMPREF1058_RS12250 0.71 -0.6 165,316 + HMPREF1058_RS12250 0.71 +0.8 165,435 + HMPREF1058_RS12250 0.79 -0.1 165,508 - HMPREF1058_RS12250 0.84 -0.3 165,611 + -0.6 165,704 + +0.1 165,749 + -0.6 165,773 + -1.3 165,774 - +0.3 165,776 - -1.9 165,776 - -0.6 165,779 + -0.3 165,780 - -0.1 165,819 + +0.3 165,835 + -0.2 165,847 + +0.0 165,852 - -1.2 165,861 + +0.1 165,862 - -1.5 165,864 - +0.0 165,865 + +0.1 165,866 - +0.2 165,866 - -0.1 165,976 - HMPREF1058_RS12245 0.39 +0.7 165,993 + HMPREF1058_RS12245 0.45 -0.5 165,994 - HMPREF1058_RS12245 0.45 -0.6 165,994 - HMPREF1058_RS12245 0.45 +0.6 166,036 + HMPREF1058_RS12245 0.60 -1.0 166,110 - HMPREF1058_RS12245 0.87 -2.4 166,110 - HMPREF1058_RS12245 0.87 -0.6 166,114 - HMPREF1058_RS12245 0.88 -1.0 166,114 - HMPREF1058_RS12245 0.88 -0.0 166,143 + -0.3 166,143 + +0.0 166,144 - -0.1 166,246 + HMPREF1058_RS12240 0.17 -0.8 166,429 - HMPREF1058_RS12240 0.47 -0.1 166,486 + HMPREF1058_RS12240 0.57 -1.4 166,486 + HMPREF1058_RS12240 0.57 -0.0 166,488 + HMPREF1058_RS12240 0.57 -1.4 166,712 + +1.5 166,741 - +0.1 166,741 - -0.2 166,777 + +0.6 166,787 - -0.3 166,893 - +0.2 167,045 - -1.0 167,080 + HMPREF1058_RS12235 0.10 +0.0 167,080 - HMPREF1058_RS12235 0.10 +0.0 167,126 + HMPREF1058_RS12235 0.12 -0.2 167,127 - HMPREF1058_RS12235 0.12 +0.7 167,137 - HMPREF1058_RS12235 0.12 -1.0 167,137 - HMPREF1058_RS12235 0.12 +0.5 167,198 + HMPREF1058_RS12235 0.14 -0.1 167,198 + HMPREF1058_RS12235 0.14 +0.2 167,199 - HMPREF1058_RS12235 0.14 -0.9 167,199 - HMPREF1058_RS12235 0.14 -0.4 167,199 - HMPREF1058_RS12235 0.14 +0.1 167,348 - HMPREF1058_RS12235 0.19 +0.4 167,414 + HMPREF1058_RS12235 0.21 +0.1 167,463 + HMPREF1058_RS12235 0.22 -0.7 167,463 + HMPREF1058_RS12235 0.22 -0.1 167,464 - HMPREF1058_RS12235 0.22 +0.4 167,464 - HMPREF1058_RS12235 0.22 -0.4 167,622 + HMPREF1058_RS12235 0.27 +0.8 167,647 - HMPREF1058_RS12235 0.28 -0.1
Or see this region's nucleotide sequence