Experiment: Brucella 1x (C) complex media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt HMPREF1058_RS21635 and HMPREF1058_RS05500 are separated by 707 nucleotides HMPREF1058_RS05500 and HMPREF1058_RS05495 are separated by 101 nucleotides
HMPREF1058_RS21635: HMPREF1058_RS21635 - transcriptional regulator, at 151,675 to 151,937
_RS21635
HMPREF1058_RS05500: HMPREF1058_RS05500 - helix-turn-helix domain-containing protein, at 152,645 to 153,118
_RS05500
HMPREF1058_RS05495: HMPREF1058_RS05495 - IS982 family transposase, at 153,220 to 154,125
_RS05495
Position (kb) 152
153
154 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 151.772 kb on - strand, within HMPREF1058_RS21635 at 151.784 kb on + strand, within HMPREF1058_RS21635 at 151.795 kb on - strand, within HMPREF1058_RS21635 at 151.941 kb on - strand at 151.946 kb on + strand at 151.963 kb on - strand at 151.967 kb on + strand at 151.967 kb on + strand at 151.968 kb on - strand at 151.970 kb on - strand at 151.973 kb on - strand at 151.976 kb on + strand at 151.977 kb on - strand at 151.991 kb on + strand at 152.008 kb on + strand at 152.019 kb on + strand at 152.033 kb on + strand at 152.205 kb on - strand at 152.245 kb on - strand at 152.369 kb on + strand at 152.387 kb on + strand at 152.388 kb on - strand at 152.518 kb on + strand at 152.605 kb on - strand at 152.720 kb on - strand, within HMPREF1058_RS05500 at 152.758 kb on + strand, within HMPREF1058_RS05500 at 152.815 kb on - strand, within HMPREF1058_RS05500 at 152.815 kb on - strand, within HMPREF1058_RS05500 at 152.878 kb on - strand, within HMPREF1058_RS05500 at 152.884 kb on - strand, within HMPREF1058_RS05500 at 152.906 kb on + strand, within HMPREF1058_RS05500 at 152.907 kb on - strand, within HMPREF1058_RS05500 at 152.908 kb on + strand, within HMPREF1058_RS05500 at 152.908 kb on + strand, within HMPREF1058_RS05500 at 152.908 kb on + strand, within HMPREF1058_RS05500 at 152.914 kb on + strand, within HMPREF1058_RS05500 at 152.960 kb on - strand, within HMPREF1058_RS05500 at 152.971 kb on + strand, within HMPREF1058_RS05500 at 152.971 kb on + strand, within HMPREF1058_RS05500 at 152.971 kb on + strand at 153.008 kb on + strand, within HMPREF1058_RS05500 at 153.009 kb on - strand, within HMPREF1058_RS05500 at 153.058 kb on + strand, within HMPREF1058_RS05500 at 153.076 kb on + strand at 153.083 kb on - strand at 153.148 kb on + strand at 153.162 kb on + strand at 153.184 kb on - strand at 153.219 kb on + strand at 153.219 kb on + strand at 153.251 kb on - strand at 153.251 kb on - strand at 153.333 kb on + strand, within HMPREF1058_RS05495 at 153.366 kb on + strand, within HMPREF1058_RS05495 at 153.377 kb on + strand, within HMPREF1058_RS05495 at 153.418 kb on + strand, within HMPREF1058_RS05495 at 153.422 kb on - strand, within HMPREF1058_RS05495 at 153.476 kb on - strand, within HMPREF1058_RS05495 at 153.525 kb on - strand, within HMPREF1058_RS05495 at 153.587 kb on + strand, within HMPREF1058_RS05495 at 153.587 kb on + strand, within HMPREF1058_RS05495 at 153.593 kb on + strand, within HMPREF1058_RS05495 at 153.594 kb on - strand, within HMPREF1058_RS05495 at 153.594 kb on - strand, within HMPREF1058_RS05495 at 153.594 kb on - strand, within HMPREF1058_RS05495 at 153.600 kb on + strand, within HMPREF1058_RS05495 at 153.821 kb on - strand, within HMPREF1058_RS05495 at 153.822 kb on + strand, within HMPREF1058_RS05495 at 153.824 kb on + strand, within HMPREF1058_RS05495 at 153.824 kb on + strand, within HMPREF1058_RS05495 at 153.864 kb on - strand, within HMPREF1058_RS05495 at 153.891 kb on + strand, within HMPREF1058_RS05495 at 154.025 kb on + strand, within HMPREF1058_RS05495 at 154.088 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Brucella 1x (C) complex media remove 151,772 - HMPREF1058_RS21635 0.37 +0.3 151,784 + HMPREF1058_RS21635 0.41 -2.0 151,795 - HMPREF1058_RS21635 0.46 -0.1 151,941 - +0.7 151,946 + -0.1 151,963 - -0.3 151,967 + +0.2 151,967 + -0.3 151,968 - +1.1 151,970 - -0.3 151,973 - -0.5 151,976 + -0.8 151,977 - -1.1 151,991 + -0.2 152,008 + +0.4 152,019 + +0.3 152,033 + +0.3 152,205 - -0.4 152,245 - +1.1 152,369 + +0.4 152,387 + +3.7 152,388 - +0.5 152,518 + +0.3 152,605 - -0.1 152,720 - HMPREF1058_RS05500 0.16 +1.0 152,758 + HMPREF1058_RS05500 0.24 -2.1 152,815 - HMPREF1058_RS05500 0.36 -0.8 152,815 - HMPREF1058_RS05500 0.36 -0.9 152,878 - HMPREF1058_RS05500 0.49 -1.3 152,884 - HMPREF1058_RS05500 0.50 -0.5 152,906 + HMPREF1058_RS05500 0.55 -0.7 152,907 - HMPREF1058_RS05500 0.55 -0.5 152,908 + HMPREF1058_RS05500 0.55 -1.7 152,908 + HMPREF1058_RS05500 0.55 +0.1 152,908 + HMPREF1058_RS05500 0.55 -1.5 152,914 + HMPREF1058_RS05500 0.57 +0.6 152,960 - HMPREF1058_RS05500 0.66 +0.4 152,971 + HMPREF1058_RS05500 0.69 -0.2 152,971 + HMPREF1058_RS05500 0.69 -0.2 152,971 + -1.1 153,008 + HMPREF1058_RS05500 0.77 -2.3 153,009 - HMPREF1058_RS05500 0.77 +0.1 153,058 + HMPREF1058_RS05500 0.87 -1.8 153,076 + +0.3 153,083 - +0.2 153,148 + +0.0 153,162 + +0.4 153,184 - +0.1 153,219 + +0.0 153,219 + +1.7 153,251 - +1.3 153,251 - +0.6 153,333 + HMPREF1058_RS05495 0.12 -1.3 153,366 + HMPREF1058_RS05495 0.16 -0.4 153,377 + HMPREF1058_RS05495 0.17 -0.3 153,418 + HMPREF1058_RS05495 0.22 -0.7 153,422 - HMPREF1058_RS05495 0.22 +0.9 153,476 - HMPREF1058_RS05495 0.28 +0.5 153,525 - HMPREF1058_RS05495 0.34 -0.2 153,587 + HMPREF1058_RS05495 0.41 -0.4 153,587 + HMPREF1058_RS05495 0.41 +1.4 153,593 + HMPREF1058_RS05495 0.41 +2.7 153,594 - HMPREF1058_RS05495 0.41 -0.4 153,594 - HMPREF1058_RS05495 0.41 -0.3 153,594 - HMPREF1058_RS05495 0.41 +1.1 153,600 + HMPREF1058_RS05495 0.42 +0.1 153,821 - HMPREF1058_RS05495 0.66 -1.0 153,822 + HMPREF1058_RS05495 0.66 -2.5 153,824 + HMPREF1058_RS05495 0.67 -0.9 153,824 + HMPREF1058_RS05495 0.67 +0.1 153,864 - HMPREF1058_RS05495 0.71 -0.1 153,891 + HMPREF1058_RS05495 0.74 +0.5 154,025 + HMPREF1058_RS05495 0.89 -0.1 154,088 + -0.1
Or see this region's nucleotide sequence