Experiment: 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_826 and Pf6N2E2_827 are separated by 541 nucleotides Pf6N2E2_827 and Pf6N2E2_828 are separated by 127 nucleotides Pf6N2E2_828 and Pf6N2E2_829 are separated by 201 nucleotides
Pf6N2E2_826 - ABC-type amino acid transport, signal transduction systems, periplasmic component/domain, at 964,711 to 965,301
_826
Pf6N2E2_827 - probable dioxygenase, at 965,843 to 966,268
_827
Pf6N2E2_828 - Transcriptional regulator, TetR family, at 966,396 to 967,016
_828
Pf6N2E2_829 - TesB-like acyl-CoA thioesterase 1, at 967,218 to 967,997
_829
Position (kb) 965
966
967 Strain fitness (log2 ratio) -2
-1
0
1
2 at 964.982 kb on + strand, within Pf6N2E2_826 at 964.982 kb on + strand, within Pf6N2E2_826 at 964.983 kb on - strand, within Pf6N2E2_826 at 964.983 kb on - strand, within Pf6N2E2_826 at 964.983 kb on - strand, within Pf6N2E2_826 at 965.020 kb on - strand, within Pf6N2E2_826 at 965.061 kb on - strand, within Pf6N2E2_826 at 965.242 kb on + strand, within Pf6N2E2_826 at 965.242 kb on + strand, within Pf6N2E2_826 at 965.242 kb on + strand, within Pf6N2E2_826 at 965.242 kb on + strand, within Pf6N2E2_826 at 965.242 kb on + strand, within Pf6N2E2_826 at 965.243 kb on - strand at 965.243 kb on - strand at 965.243 kb on - strand at 965.243 kb on - strand at 965.243 kb on - strand at 965.255 kb on - strand at 965.255 kb on - strand at 965.463 kb on + strand at 965.542 kb on + strand at 965.542 kb on + strand at 965.543 kb on - strand at 965.543 kb on - strand at 965.543 kb on - strand at 965.543 kb on - strand at 965.660 kb on + strand at 965.660 kb on + strand at 965.660 kb on + strand at 965.660 kb on + strand at 965.661 kb on - strand at 965.661 kb on - strand at 965.662 kb on + strand at 965.662 kb on + strand at 965.663 kb on - strand at 965.663 kb on - strand at 965.663 kb on - strand at 965.663 kb on - strand at 965.701 kb on + strand at 965.701 kb on + strand at 965.701 kb on + strand at 965.702 kb on - strand at 965.830 kb on - strand at 965.879 kb on + strand at 965.907 kb on - strand, within Pf6N2E2_827 at 965.907 kb on - strand, within Pf6N2E2_827 at 965.970 kb on - strand, within Pf6N2E2_827 at 966.152 kb on + strand, within Pf6N2E2_827 at 966.384 kb on + strand at 966.384 kb on + strand at 966.496 kb on - strand, within Pf6N2E2_828 at 966.555 kb on + strand, within Pf6N2E2_828 at 966.555 kb on + strand, within Pf6N2E2_828 at 966.556 kb on - strand, within Pf6N2E2_828 at 966.556 kb on - strand, within Pf6N2E2_828 at 966.556 kb on - strand, within Pf6N2E2_828 at 966.561 kb on + strand, within Pf6N2E2_828 at 966.562 kb on - strand, within Pf6N2E2_828 at 966.747 kb on + strand, within Pf6N2E2_828 at 966.747 kb on + strand, within Pf6N2E2_828 at 966.799 kb on - strand, within Pf6N2E2_828 at 966.934 kb on + strand, within Pf6N2E2_828 at 966.945 kb on + strand, within Pf6N2E2_828 at 966.945 kb on + strand, within Pf6N2E2_828 at 967.077 kb on - strand at 967.142 kb on + strand at 967.215 kb on + strand at 967.216 kb on - strand at 967.216 kb on - strand at 967.216 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM putrescine remove 964,982 + Pf6N2E2_826 0.46 -0.5 964,982 + Pf6N2E2_826 0.46 -0.5 964,983 - Pf6N2E2_826 0.46 -0.5 964,983 - Pf6N2E2_826 0.46 -1.0 964,983 - Pf6N2E2_826 0.46 +0.3 965,020 - Pf6N2E2_826 0.52 -1.5 965,061 - Pf6N2E2_826 0.59 +1.0 965,242 + Pf6N2E2_826 0.90 +0.9 965,242 + Pf6N2E2_826 0.90 +0.5 965,242 + Pf6N2E2_826 0.90 -0.1 965,242 + Pf6N2E2_826 0.90 +0.8 965,242 + Pf6N2E2_826 0.90 -0.5 965,243 - +0.0 965,243 - -0.2 965,243 - +0.5 965,243 - -0.5 965,243 - -1.5 965,255 - +0.0 965,255 - +1.5 965,463 + +0.3 965,542 + -0.5 965,542 + +0.5 965,543 - +0.3 965,543 - -0.8 965,543 - +0.3 965,543 - -0.3 965,660 + +1.2 965,660 + -0.5 965,660 + -1.1 965,660 + -1.1 965,661 - +0.0 965,661 - -0.5 965,662 + -0.7 965,662 + +0.2 965,663 - +0.0 965,663 - -0.1 965,663 - +0.0 965,663 - -0.4 965,701 + +0.1 965,701 + -2.1 965,701 + -0.5 965,702 - -0.5 965,830 - -0.5 965,879 + +0.5 965,907 - Pf6N2E2_827 0.15 -1.8 965,907 - Pf6N2E2_827 0.15 +0.2 965,970 - Pf6N2E2_827 0.30 -1.5 966,152 + Pf6N2E2_827 0.73 -0.7 966,384 + +1.5 966,384 + +1.0 966,496 - Pf6N2E2_828 0.16 +0.8 966,555 + Pf6N2E2_828 0.26 -0.1 966,555 + Pf6N2E2_828 0.26 +0.3 966,556 - Pf6N2E2_828 0.26 -1.0 966,556 - Pf6N2E2_828 0.26 -0.5 966,556 - Pf6N2E2_828 0.26 +0.5 966,561 + Pf6N2E2_828 0.27 +1.2 966,562 - Pf6N2E2_828 0.27 +2.0 966,747 + Pf6N2E2_828 0.57 -0.1 966,747 + Pf6N2E2_828 0.57 -0.3 966,799 - Pf6N2E2_828 0.65 +1.0 966,934 + Pf6N2E2_828 0.87 -2.5 966,945 + Pf6N2E2_828 0.88 -0.2 966,945 + Pf6N2E2_828 0.88 +0.5 967,077 - +0.9 967,142 + -0.1 967,215 + +1.0 967,216 - -0.2 967,216 - +0.2 967,216 - +0.5
Or see this region's nucleotide sequence