Experiment: 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5879 and Pf6N2E2_5880 overlap by 4 nucleotides Pf6N2E2_5880 and Pf6N2E2_5881 are separated by 129 nucleotides Pf6N2E2_5881 and Pf6N2E2_5882 are separated by 64 nucleotides
Pf6N2E2_5879 - FIG00955363: hypothetical protein, at 6,699,284 to 6,700,078
_5879
Pf6N2E2_5880 - hydrolase, alpha/beta fold family, at 6,700,075 to 6,700,926
_5880
Pf6N2E2_5881 - Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit, at 6,701,056 to 6,701,874
_5881
Pf6N2E2_5882 - Putative membrane protein, at 6,701,939 to 6,702,976
_5882
Position (kb)
6701
6702 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6700.160 kb on + strand at 6700.310 kb on - strand, within Pf6N2E2_5880 at 6700.310 kb on - strand, within Pf6N2E2_5880 at 6700.549 kb on + strand, within Pf6N2E2_5880 at 6700.581 kb on + strand, within Pf6N2E2_5880 at 6700.623 kb on - strand, within Pf6N2E2_5880 at 6700.660 kb on + strand, within Pf6N2E2_5880 at 6700.661 kb on - strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.662 kb on + strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.663 kb on - strand, within Pf6N2E2_5880 at 6700.856 kb on + strand at 6700.856 kb on + strand at 6700.856 kb on + strand at 6700.856 kb on + strand at 6700.856 kb on + strand at 6700.857 kb on - strand at 6700.857 kb on - strand at 6700.857 kb on - strand at 6700.857 kb on - strand at 6700.890 kb on - strand at 6700.993 kb on - strand at 6701.218 kb on + strand, within Pf6N2E2_5881 at 6701.218 kb on + strand, within Pf6N2E2_5881 at 6701.218 kb on + strand, within Pf6N2E2_5881 at 6701.219 kb on - strand, within Pf6N2E2_5881 at 6701.301 kb on + strand, within Pf6N2E2_5881 at 6701.301 kb on + strand, within Pf6N2E2_5881 at 6701.302 kb on - strand, within Pf6N2E2_5881 at 6701.302 kb on - strand, within Pf6N2E2_5881 at 6701.305 kb on + strand, within Pf6N2E2_5881 at 6701.305 kb on + strand, within Pf6N2E2_5881 at 6701.305 kb on + strand, within Pf6N2E2_5881 at 6701.305 kb on + strand, within Pf6N2E2_5881 at 6701.305 kb on + strand, within Pf6N2E2_5881 at 6701.305 kb on + strand, within Pf6N2E2_5881 at 6701.305 kb on + strand, within Pf6N2E2_5881 at 6701.306 kb on - strand, within Pf6N2E2_5881 at 6701.343 kb on + strand, within Pf6N2E2_5881 at 6701.344 kb on - strand, within Pf6N2E2_5881 at 6701.461 kb on + strand, within Pf6N2E2_5881 at 6701.461 kb on + strand, within Pf6N2E2_5881 at 6701.462 kb on - strand, within Pf6N2E2_5881 at 6701.462 kb on - strand, within Pf6N2E2_5881 at 6701.462 kb on - strand, within Pf6N2E2_5881 at 6701.462 kb on - strand, within Pf6N2E2_5881 at 6701.536 kb on + strand, within Pf6N2E2_5881 at 6701.536 kb on + strand, within Pf6N2E2_5881 at 6701.536 kb on + strand, within Pf6N2E2_5881 at 6701.536 kb on + strand, within Pf6N2E2_5881 at 6701.536 kb on + strand, within Pf6N2E2_5881 at 6701.536 kb on + strand, within Pf6N2E2_5881 at 6701.537 kb on - strand, within Pf6N2E2_5881 at 6701.537 kb on - strand, within Pf6N2E2_5881 at 6701.566 kb on + strand, within Pf6N2E2_5881 at 6701.566 kb on + strand, within Pf6N2E2_5881 at 6701.567 kb on - strand, within Pf6N2E2_5881 at 6701.873 kb on - strand at 6701.936 kb on + strand at 6701.936 kb on + strand at 6701.936 kb on + strand at 6701.936 kb on + strand at 6701.936 kb on + strand at 6701.936 kb on + strand at 6701.936 kb on + strand at 6701.936 kb on + strand at 6702.132 kb on + strand, within Pf6N2E2_5882 at 6702.133 kb on - strand, within Pf6N2E2_5882 at 6702.133 kb on - strand, within Pf6N2E2_5882 at 6702.354 kb on - strand, within Pf6N2E2_5882 at 6702.354 kb on - strand, within Pf6N2E2_5882 at 6702.430 kb on + strand, within Pf6N2E2_5882 at 6702.430 kb on + strand, within Pf6N2E2_5882 at 6702.452 kb on + strand, within Pf6N2E2_5882 at 6702.528 kb on - strand, within Pf6N2E2_5882 at 6702.528 kb on - strand, within Pf6N2E2_5882 at 6702.528 kb on - strand, within Pf6N2E2_5882 at 6702.528 kb on - strand, within Pf6N2E2_5882 at 6702.815 kb on + strand, within Pf6N2E2_5882
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM putrescine remove 6,700,160 + +0.4 6,700,310 - Pf6N2E2_5880 0.28 +0.8 6,700,310 - Pf6N2E2_5880 0.28 +0.7 6,700,549 + Pf6N2E2_5880 0.56 +0.1 6,700,581 + Pf6N2E2_5880 0.59 -0.0 6,700,623 - Pf6N2E2_5880 0.64 -0.6 6,700,660 + Pf6N2E2_5880 0.69 -0.5 6,700,661 - Pf6N2E2_5880 0.69 -0.2 6,700,662 + Pf6N2E2_5880 0.69 -2.0 6,700,662 + Pf6N2E2_5880 0.69 -0.0 6,700,662 + Pf6N2E2_5880 0.69 -0.0 6,700,662 + Pf6N2E2_5880 0.69 -0.1 6,700,662 + Pf6N2E2_5880 0.69 -1.0 6,700,662 + Pf6N2E2_5880 0.69 -0.7 6,700,662 + Pf6N2E2_5880 0.69 -0.7 6,700,662 + Pf6N2E2_5880 0.69 -0.4 6,700,662 + Pf6N2E2_5880 0.69 +1.2 6,700,662 + Pf6N2E2_5880 0.69 -0.2 6,700,662 + Pf6N2E2_5880 0.69 +0.6 6,700,663 - Pf6N2E2_5880 0.69 +2.0 6,700,663 - Pf6N2E2_5880 0.69 -1.2 6,700,663 - Pf6N2E2_5880 0.69 -0.2 6,700,663 - Pf6N2E2_5880 0.69 +1.0 6,700,663 - Pf6N2E2_5880 0.69 +1.0 6,700,663 - Pf6N2E2_5880 0.69 +1.0 6,700,663 - Pf6N2E2_5880 0.69 -0.2 6,700,663 - Pf6N2E2_5880 0.69 +0.8 6,700,663 - Pf6N2E2_5880 0.69 -2.6 6,700,663 - Pf6N2E2_5880 0.69 -0.6 6,700,663 - Pf6N2E2_5880 0.69 +1.0 6,700,663 - Pf6N2E2_5880 0.69 -0.0 6,700,663 - Pf6N2E2_5880 0.69 -1.0 6,700,663 - Pf6N2E2_5880 0.69 +0.8 6,700,663 - Pf6N2E2_5880 0.69 -0.6 6,700,663 - Pf6N2E2_5880 0.69 -0.0 6,700,663 - Pf6N2E2_5880 0.69 +0.2 6,700,663 - Pf6N2E2_5880 0.69 -0.6 6,700,856 + +0.3 6,700,856 + -0.0 6,700,856 + -0.7 6,700,856 + -0.5 6,700,856 + +1.0 6,700,857 - -1.0 6,700,857 - +1.0 6,700,857 - -0.2 6,700,857 - -1.3 6,700,890 - +0.7 6,700,993 - -0.0 6,701,218 + Pf6N2E2_5881 0.20 -0.0 6,701,218 + Pf6N2E2_5881 0.20 +1.6 6,701,218 + Pf6N2E2_5881 0.20 +0.6 6,701,219 - Pf6N2E2_5881 0.20 -0.0 6,701,301 + Pf6N2E2_5881 0.30 +0.6 6,701,301 + Pf6N2E2_5881 0.30 +0.7 6,701,302 - Pf6N2E2_5881 0.30 +0.3 6,701,302 - Pf6N2E2_5881 0.30 +0.3 6,701,305 + Pf6N2E2_5881 0.30 +0.2 6,701,305 + Pf6N2E2_5881 0.30 -1.0 6,701,305 + Pf6N2E2_5881 0.30 -0.2 6,701,305 + Pf6N2E2_5881 0.30 +1.0 6,701,305 + Pf6N2E2_5881 0.30 +2.3 6,701,305 + Pf6N2E2_5881 0.30 +2.6 6,701,305 + Pf6N2E2_5881 0.30 -0.8 6,701,306 - Pf6N2E2_5881 0.31 -0.0 6,701,343 + Pf6N2E2_5881 0.35 -2.0 6,701,344 - Pf6N2E2_5881 0.35 +1.4 6,701,461 + Pf6N2E2_5881 0.49 +1.4 6,701,461 + Pf6N2E2_5881 0.49 +1.4 6,701,462 - Pf6N2E2_5881 0.50 +0.4 6,701,462 - Pf6N2E2_5881 0.50 +0.7 6,701,462 - Pf6N2E2_5881 0.50 +1.4 6,701,462 - Pf6N2E2_5881 0.50 +0.6 6,701,536 + Pf6N2E2_5881 0.59 +0.8 6,701,536 + Pf6N2E2_5881 0.59 -0.0 6,701,536 + Pf6N2E2_5881 0.59 +1.0 6,701,536 + Pf6N2E2_5881 0.59 -0.6 6,701,536 + Pf6N2E2_5881 0.59 +0.3 6,701,536 + Pf6N2E2_5881 0.59 +0.4 6,701,537 - Pf6N2E2_5881 0.59 -0.0 6,701,537 - Pf6N2E2_5881 0.59 +1.0 6,701,566 + Pf6N2E2_5881 0.62 +0.7 6,701,566 + Pf6N2E2_5881 0.62 -0.0 6,701,567 - Pf6N2E2_5881 0.62 -0.6 6,701,873 - +0.6 6,701,936 + -0.0 6,701,936 + -0.7 6,701,936 + +1.0 6,701,936 + -0.1 6,701,936 + +0.2 6,701,936 + -0.8 6,701,936 + +1.0 6,701,936 + +0.5 6,702,132 + Pf6N2E2_5882 0.19 -0.0 6,702,133 - Pf6N2E2_5882 0.19 -0.2 6,702,133 - Pf6N2E2_5882 0.19 -0.5 6,702,354 - Pf6N2E2_5882 0.40 -1.0 6,702,354 - Pf6N2E2_5882 0.40 -0.0 6,702,430 + Pf6N2E2_5882 0.47 +1.0 6,702,430 + Pf6N2E2_5882 0.47 -0.0 6,702,452 + Pf6N2E2_5882 0.49 -0.0 6,702,528 - Pf6N2E2_5882 0.57 +0.5 6,702,528 - Pf6N2E2_5882 0.57 -0.9 6,702,528 - Pf6N2E2_5882 0.57 +0.3 6,702,528 - Pf6N2E2_5882 0.57 -0.0 6,702,815 + Pf6N2E2_5882 0.84 +0.6
Or see this region's nucleotide sequence