Experiment: 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5011 and Pf6N2E2_5012 are separated by 117 nucleotides Pf6N2E2_5012 and Pf6N2E2_5013 are separated by 103 nucleotides
Pf6N2E2_5011 - Leucyl-tRNA synthetase (EC 6.1.1.4), at 5,776,317 to 5,779,109
_5011
Pf6N2E2_5012 - FIG00954871: hypothetical protein, at 5,779,227 to 5,779,547
_5012
Pf6N2E2_5013 - Putative membrane protein, at 5,779,651 to 5,780,412
_5013
Position (kb) 5779
5780 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 5779.033 kb on - strand at 5779.181 kb on + strand at 5779.181 kb on + strand at 5779.182 kb on - strand at 5779.182 kb on - strand at 5779.182 kb on - strand at 5779.182 kb on - strand at 5779.207 kb on + strand at 5779.207 kb on + strand at 5779.208 kb on - strand at 5779.208 kb on - strand at 5779.346 kb on - strand, within Pf6N2E2_5012 at 5779.399 kb on - strand, within Pf6N2E2_5012 at 5779.399 kb on - strand, within Pf6N2E2_5012 at 5779.418 kb on + strand, within Pf6N2E2_5012 at 5779.418 kb on + strand, within Pf6N2E2_5012 at 5779.419 kb on - strand, within Pf6N2E2_5012 at 5779.419 kb on - strand, within Pf6N2E2_5012 at 5779.444 kb on + strand, within Pf6N2E2_5012 at 5779.444 kb on + strand, within Pf6N2E2_5012 at 5779.508 kb on + strand, within Pf6N2E2_5012 at 5779.519 kb on + strand at 5779.520 kb on - strand at 5779.520 kb on - strand at 5779.520 kb on - strand at 5779.545 kb on + strand at 5779.545 kb on + strand at 5779.545 kb on + strand at 5779.546 kb on - strand at 5779.546 kb on - strand at 5779.546 kb on - strand at 5779.623 kb on - strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.637 kb on + strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.638 kb on - strand at 5779.664 kb on - strand at 5779.682 kb on + strand at 5779.682 kb on + strand at 5779.682 kb on + strand at 5779.682 kb on + strand at 5779.683 kb on - strand at 5779.683 kb on - strand at 5779.683 kb on - strand at 5779.683 kb on - strand at 5779.956 kb on + strand, within Pf6N2E2_5013 at 5779.956 kb on + strand, within Pf6N2E2_5013 at 5780.044 kb on + strand, within Pf6N2E2_5013 at 5780.130 kb on + strand, within Pf6N2E2_5013 at 5780.404 kb on + strand at 5780.405 kb on - strand at 5780.434 kb on + strand at 5780.453 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM putrescine remove 5,779,033 - +0.3 5,779,181 + +0.1 5,779,181 + +0.3 5,779,182 - +0.3 5,779,182 - -1.4 5,779,182 - -3.4 5,779,182 - -0.2 5,779,207 + -1.5 5,779,207 + -0.5 5,779,208 - -1.0 5,779,208 - +0.3 5,779,346 - Pf6N2E2_5012 0.37 -1.2 5,779,399 - Pf6N2E2_5012 0.54 -1.0 5,779,399 - Pf6N2E2_5012 0.54 -1.3 5,779,418 + Pf6N2E2_5012 0.60 -2.3 5,779,418 + Pf6N2E2_5012 0.60 +0.3 5,779,419 - Pf6N2E2_5012 0.60 -1.5 5,779,419 - Pf6N2E2_5012 0.60 +1.3 5,779,444 + Pf6N2E2_5012 0.68 +0.1 5,779,444 + Pf6N2E2_5012 0.68 -0.5 5,779,508 + Pf6N2E2_5012 0.88 -1.1 5,779,519 + +0.0 5,779,520 - -0.9 5,779,520 - +0.3 5,779,520 - +0.9 5,779,545 + -0.2 5,779,545 + -0.6 5,779,545 + -0.2 5,779,546 - +0.3 5,779,546 - -0.8 5,779,546 - -0.7 5,779,623 - -0.2 5,779,637 + -0.3 5,779,637 + -1.4 5,779,637 + -0.5 5,779,637 + -1.0 5,779,637 + -0.6 5,779,637 + -0.3 5,779,637 + -1.3 5,779,637 + -1.5 5,779,637 + -0.5 5,779,637 + +1.6 5,779,637 + +0.0 5,779,637 + -1.6 5,779,638 - -0.9 5,779,638 - -1.2 5,779,638 - -0.6 5,779,638 - -0.7 5,779,638 - -1.2 5,779,638 - -0.4 5,779,638 - -0.0 5,779,638 - -0.8 5,779,664 - -0.7 5,779,682 + +0.5 5,779,682 + -0.3 5,779,682 + +0.6 5,779,682 + +0.3 5,779,683 - -1.8 5,779,683 - -1.3 5,779,683 - -0.3 5,779,683 - +1.0 5,779,956 + Pf6N2E2_5013 0.40 +1.3 5,779,956 + Pf6N2E2_5013 0.40 -0.1 5,780,044 + Pf6N2E2_5013 0.52 +0.3 5,780,130 + Pf6N2E2_5013 0.63 +1.5 5,780,404 + -0.2 5,780,405 - +0.7 5,780,434 + -0.1 5,780,453 + -0.1
Or see this region's nucleotide sequence