Experiment: 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_383 and Pf6N2E2_384 are separated by 58 nucleotides Pf6N2E2_384 and Pf6N2E2_385 are separated by 97 nucleotides Pf6N2E2_385 and Pf6N2E2_386 are separated by 460 nucleotides
Pf6N2E2_383 - 3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100), at 450,812 to 451,579
_383
Pf6N2E2_384 - Glycine cleavage system transcriptional activator, at 451,638 to 451,979
_384
Pf6N2E2_385 - hypothetical protein, at 452,077 to 452,247
_385
Pf6N2E2_386 - similar to ribulose-1,5-bisphosphate carboxylase, Type III, at 452,708 to 453,985
_386
Position (kb) 451
452 Strain fitness (log2 ratio) -2
-1
0
1
2 at 450.757 kb on + strand at 450.792 kb on + strand at 450.795 kb on + strand at 450.795 kb on + strand at 450.795 kb on + strand at 450.795 kb on + strand at 450.796 kb on - strand at 450.840 kb on + strand at 450.857 kb on - strand at 450.909 kb on + strand, within Pf6N2E2_383 at 450.909 kb on + strand, within Pf6N2E2_383 at 450.910 kb on - strand, within Pf6N2E2_383 at 450.929 kb on - strand, within Pf6N2E2_383 at 451.201 kb on + strand, within Pf6N2E2_383 at 451.201 kb on + strand, within Pf6N2E2_383 at 451.201 kb on + strand, within Pf6N2E2_383 at 451.202 kb on - strand, within Pf6N2E2_383 at 451.427 kb on + strand, within Pf6N2E2_383 at 451.428 kb on - strand, within Pf6N2E2_383 at 451.490 kb on + strand, within Pf6N2E2_383 at 451.490 kb on + strand, within Pf6N2E2_383 at 451.491 kb on - strand, within Pf6N2E2_383 at 451.515 kb on - strand at 451.515 kb on - strand at 451.590 kb on + strand at 451.591 kb on - strand at 451.597 kb on - strand at 451.616 kb on + strand at 451.616 kb on + strand at 451.660 kb on - strand at 451.852 kb on + strand at 451.853 kb on - strand, within Pf6N2E2_384 at 451.853 kb on - strand, within Pf6N2E2_384 at 451.913 kb on - strand, within Pf6N2E2_384 at 452.005 kb on + strand at 452.005 kb on + strand at 452.006 kb on - strand at 452.019 kb on + strand at 452.019 kb on + strand at 452.020 kb on - strand at 452.020 kb on - strand at 452.021 kb on + strand at 452.021 kb on + strand at 452.022 kb on - strand at 452.022 kb on - strand at 452.264 kb on + strand at 452.405 kb on + strand at 452.635 kb on - strand at 452.635 kb on - strand at 452.637 kb on - strand at 452.678 kb on - strand at 452.678 kb on - strand at 452.679 kb on + strand at 452.758 kb on - strand at 452.819 kb on - strand at 452.819 kb on - strand at 452.919 kb on + strand, within Pf6N2E2_386 at 452.924 kb on - strand, within Pf6N2E2_386 at 452.931 kb on - strand, within Pf6N2E2_386 at 452.931 kb on - strand, within Pf6N2E2_386 at 452.931 kb on - strand, within Pf6N2E2_386 at 452.931 kb on - strand, within Pf6N2E2_386 at 452.960 kb on + strand, within Pf6N2E2_386 at 452.960 kb on + strand, within Pf6N2E2_386 at 452.960 kb on + strand, within Pf6N2E2_386 at 452.960 kb on + strand, within Pf6N2E2_386 at 452.961 kb on - strand, within Pf6N2E2_386 at 452.961 kb on - strand, within Pf6N2E2_386 at 452.964 kb on - strand, within Pf6N2E2_386
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM putrescine remove 450,757 + +0.9 450,792 + -0.5 450,795 + +1.0 450,795 + -0.5 450,795 + +0.1 450,795 + +0.4 450,796 - -0.2 450,840 + -0.1 450,857 - -0.3 450,909 + Pf6N2E2_383 0.13 -1.9 450,909 + Pf6N2E2_383 0.13 -1.5 450,910 - Pf6N2E2_383 0.13 -0.3 450,929 - Pf6N2E2_383 0.15 -0.3 451,201 + Pf6N2E2_383 0.51 -0.4 451,201 + Pf6N2E2_383 0.51 -1.9 451,201 + Pf6N2E2_383 0.51 +0.9 451,202 - Pf6N2E2_383 0.51 +2.0 451,427 + Pf6N2E2_383 0.80 -0.5 451,428 - Pf6N2E2_383 0.80 +0.7 451,490 + Pf6N2E2_383 0.88 +0.2 451,490 + Pf6N2E2_383 0.88 +0.4 451,491 - Pf6N2E2_383 0.88 -0.7 451,515 - -0.4 451,515 - -1.3 451,590 + -0.3 451,591 - +2.3 451,597 - -0.9 451,616 + -0.8 451,616 + -0.3 451,660 - -0.3 451,852 + -0.3 451,853 - Pf6N2E2_384 0.63 -1.1 451,853 - Pf6N2E2_384 0.63 -0.3 451,913 - Pf6N2E2_384 0.80 -1.0 452,005 + +0.7 452,005 + -0.3 452,006 - -0.1 452,019 + -0.3 452,019 + -1.3 452,020 - -0.6 452,020 - +0.4 452,021 + +0.9 452,021 + +1.7 452,022 - -0.3 452,022 - -0.3 452,264 + -0.3 452,405 + -0.3 452,635 - +0.4 452,635 - -0.3 452,637 - -0.1 452,678 - -0.1 452,678 - +0.7 452,679 + +0.3 452,758 - -1.3 452,819 - +1.5 452,819 - +0.3 452,919 + Pf6N2E2_386 0.17 -1.3 452,924 - Pf6N2E2_386 0.17 -0.8 452,931 - Pf6N2E2_386 0.17 -0.1 452,931 - Pf6N2E2_386 0.17 -0.6 452,931 - Pf6N2E2_386 0.17 +0.7 452,931 - Pf6N2E2_386 0.17 +0.7 452,960 + Pf6N2E2_386 0.20 -0.3 452,960 + Pf6N2E2_386 0.20 -1.3 452,960 + Pf6N2E2_386 0.20 +0.7 452,960 + Pf6N2E2_386 0.20 -1.3 452,961 - Pf6N2E2_386 0.20 -0.3 452,961 - Pf6N2E2_386 0.20 -0.0 452,964 - Pf6N2E2_386 0.20 -0.9
Or see this region's nucleotide sequence