Experiment: 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_354 and Pf6N2E2_355 are separated by 152 nucleotides Pf6N2E2_355 and Pf6N2E2_356 are separated by 126 nucleotides
Pf6N2E2_354 - Type III PLP / low-specificity D-threonine aldolase, at 419,632 to 420,711
_354
Pf6N2E2_355 - Transcriptional regulator, LysR family, at 420,864 to 421,748
_355
Pf6N2E2_356 - hypothetical protein, at 421,875 to 422,822
_356
Position (kb) 420
421
422 Strain fitness (log2 ratio) -1
0
1
2 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.059 kb on + strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.060 kb on - strand, within Pf6N2E2_354 at 420.242 kb on + strand, within Pf6N2E2_354 at 420.242 kb on + strand, within Pf6N2E2_354 at 420.243 kb on - strand, within Pf6N2E2_354 at 420.521 kb on + strand, within Pf6N2E2_354 at 420.522 kb on - strand, within Pf6N2E2_354 at 420.522 kb on - strand at 420.700 kb on - strand at 420.721 kb on - strand at 420.804 kb on + strand at 420.804 kb on + strand at 420.805 kb on - strand at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.225 kb on + strand, within Pf6N2E2_355 at 421.226 kb on - strand, within Pf6N2E2_355 at 421.226 kb on - strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.427 kb on + strand, within Pf6N2E2_355 at 421.428 kb on - strand, within Pf6N2E2_355 at 421.428 kb on - strand, within Pf6N2E2_355 at 421.857 kb on - strand at 421.857 kb on - strand at 421.857 kb on - strand at 421.857 kb on - strand at 421.877 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM putrescine remove 420,059 + Pf6N2E2_354 0.40 -1.1 420,059 + Pf6N2E2_354 0.40 -0.6 420,059 + Pf6N2E2_354 0.40 +0.1 420,059 + Pf6N2E2_354 0.40 +2.3 420,059 + Pf6N2E2_354 0.40 -0.3 420,060 - Pf6N2E2_354 0.40 +1.3 420,060 - Pf6N2E2_354 0.40 +0.7 420,060 - Pf6N2E2_354 0.40 +0.7 420,242 + Pf6N2E2_354 0.56 +0.1 420,242 + Pf6N2E2_354 0.56 +1.5 420,243 - Pf6N2E2_354 0.57 -0.1 420,521 + Pf6N2E2_354 0.82 +0.2 420,522 - Pf6N2E2_354 0.82 -0.7 420,522 - -0.3 420,700 - -0.3 420,721 - +1.1 420,804 + -0.3 420,804 + +0.0 420,805 - -0.5 421,225 + Pf6N2E2_355 0.41 +0.4 421,225 + Pf6N2E2_355 0.41 -0.3 421,225 + Pf6N2E2_355 0.41 +0.4 421,225 + Pf6N2E2_355 0.41 -0.3 421,225 + Pf6N2E2_355 0.41 -0.7 421,226 - Pf6N2E2_355 0.41 -0.3 421,226 - Pf6N2E2_355 0.41 +0.3 421,427 + Pf6N2E2_355 0.64 +0.4 421,427 + Pf6N2E2_355 0.64 +0.5 421,427 + Pf6N2E2_355 0.64 -0.0 421,427 + Pf6N2E2_355 0.64 +0.5 421,427 + Pf6N2E2_355 0.64 +0.3 421,428 - Pf6N2E2_355 0.64 -1.3 421,428 - Pf6N2E2_355 0.64 +1.8 421,857 - -1.2 421,857 - +0.2 421,857 - -0.9 421,857 - -1.4 421,877 - +0.9
Or see this region's nucleotide sequence