Experiment: 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3421 and Pf6N2E2_3422 are separated by 159 nucleotides Pf6N2E2_3422 and Pf6N2E2_3423 are separated by 3 nucleotides Pf6N2E2_3423 and Pf6N2E2_3424 overlap by 4 nucleotides
Pf6N2E2_3421 - Transcriptional regulator ahyR/asaR family, at 3,978,349 to 3,979,110
_3421
Pf6N2E2_3422 - Proline iminopeptidase (EC 3.4.11.5), at 3,979,270 to 3,980,160
_3422
Pf6N2E2_3423 - Putative glutathione transporter, permease component, at 3,980,164 to 3,981,111
_3423
Pf6N2E2_3424 - Dipeptide transport system permease protein DppC (TC 3.A.1.5.2), at 3,981,108 to 3,981,965
_3424
Position (kb)
3979
3980
3981 Strain fitness (log2 ratio)
-1
0
1
2 at 3978.273 kb on + strand at 3978.274 kb on - strand at 3978.316 kb on + strand at 3978.316 kb on + strand at 3978.316 kb on + strand at 3978.316 kb on + strand at 3978.320 kb on + strand at 3978.321 kb on - strand at 3978.321 kb on - strand at 3978.321 kb on - strand at 3978.333 kb on - strand at 3978.755 kb on + strand, within Pf6N2E2_3421 at 3978.755 kb on + strand, within Pf6N2E2_3421 at 3978.756 kb on - strand, within Pf6N2E2_3421 at 3978.756 kb on - strand, within Pf6N2E2_3421 at 3978.881 kb on + strand, within Pf6N2E2_3421 at 3978.881 kb on + strand, within Pf6N2E2_3421 at 3978.884 kb on + strand, within Pf6N2E2_3421 at 3978.884 kb on + strand, within Pf6N2E2_3421 at 3978.924 kb on + strand, within Pf6N2E2_3421 at 3978.989 kb on + strand, within Pf6N2E2_3421 at 3978.990 kb on - strand, within Pf6N2E2_3421 at 3978.990 kb on - strand, within Pf6N2E2_3421 at 3978.995 kb on + strand, within Pf6N2E2_3421 at 3978.995 kb on + strand, within Pf6N2E2_3421 at 3978.995 kb on + strand, within Pf6N2E2_3421 at 3978.996 kb on - strand, within Pf6N2E2_3421 at 3979.154 kb on + strand at 3979.154 kb on + strand at 3979.194 kb on + strand at 3979.194 kb on + strand at 3979.194 kb on + strand at 3979.195 kb on - strand at 3979.195 kb on - strand at 3979.195 kb on - strand at 3979.206 kb on + strand at 3979.206 kb on + strand at 3979.206 kb on + strand at 3979.206 kb on + strand at 3979.206 kb on - strand at 3979.207 kb on - strand at 3979.211 kb on + strand at 3979.211 kb on + strand at 3979.212 kb on - strand at 3979.221 kb on + strand at 3979.234 kb on - strand at 3979.240 kb on + strand at 3979.241 kb on - strand at 3979.340 kb on - strand at 3979.406 kb on - strand, within Pf6N2E2_3422 at 3979.406 kb on - strand, within Pf6N2E2_3422 at 3979.494 kb on + strand, within Pf6N2E2_3422 at 3979.538 kb on + strand, within Pf6N2E2_3422 at 3979.538 kb on + strand, within Pf6N2E2_3422 at 3979.618 kb on + strand, within Pf6N2E2_3422 at 3979.619 kb on - strand, within Pf6N2E2_3422 at 3979.666 kb on - strand, within Pf6N2E2_3422 at 3979.678 kb on - strand, within Pf6N2E2_3422 at 3979.678 kb on - strand, within Pf6N2E2_3422 at 3979.786 kb on + strand, within Pf6N2E2_3422 at 3979.787 kb on - strand, within Pf6N2E2_3422 at 3979.813 kb on + strand, within Pf6N2E2_3422 at 3979.814 kb on - strand, within Pf6N2E2_3422 at 3979.814 kb on - strand, within Pf6N2E2_3422 at 3979.814 kb on - strand, within Pf6N2E2_3422 at 3979.814 kb on - strand, within Pf6N2E2_3422 at 3979.888 kb on + strand, within Pf6N2E2_3422 at 3979.888 kb on + strand, within Pf6N2E2_3422 at 3979.888 kb on + strand, within Pf6N2E2_3422 at 3979.889 kb on - strand, within Pf6N2E2_3422 at 3979.924 kb on - strand, within Pf6N2E2_3422 at 3979.989 kb on + strand, within Pf6N2E2_3422 at 3979.990 kb on - strand, within Pf6N2E2_3422 at 3980.057 kb on + strand, within Pf6N2E2_3422 at 3980.057 kb on + strand, within Pf6N2E2_3422 at 3980.058 kb on - strand, within Pf6N2E2_3422 at 3980.058 kb on - strand, within Pf6N2E2_3422 at 3980.058 kb on - strand, within Pf6N2E2_3422 at 3980.116 kb on + strand at 3980.117 kb on - strand at 3980.159 kb on - strand at 3980.189 kb on + strand at 3980.190 kb on - strand at 3980.205 kb on - strand at 3980.210 kb on + strand at 3980.210 kb on + strand at 3980.211 kb on - strand at 3980.211 kb on - strand at 3980.339 kb on - strand, within Pf6N2E2_3423 at 3980.368 kb on + strand, within Pf6N2E2_3423 at 3980.368 kb on + strand, within Pf6N2E2_3423 at 3980.369 kb on - strand, within Pf6N2E2_3423 at 3980.369 kb on - strand, within Pf6N2E2_3423 at 3980.374 kb on + strand, within Pf6N2E2_3423 at 3980.374 kb on + strand, within Pf6N2E2_3423 at 3980.374 kb on + strand, within Pf6N2E2_3423 at 3980.375 kb on - strand, within Pf6N2E2_3423 at 3980.375 kb on - strand, within Pf6N2E2_3423 at 3980.375 kb on - strand, within Pf6N2E2_3423 at 3980.422 kb on + strand, within Pf6N2E2_3423 at 3980.423 kb on - strand, within Pf6N2E2_3423 at 3980.451 kb on + strand, within Pf6N2E2_3423 at 3980.790 kb on - strand, within Pf6N2E2_3423 at 3980.790 kb on - strand, within Pf6N2E2_3423 at 3980.790 kb on - strand, within Pf6N2E2_3423 at 3980.844 kb on - strand, within Pf6N2E2_3423 at 3980.978 kb on + strand, within Pf6N2E2_3423 at 3980.992 kb on - strand, within Pf6N2E2_3423 at 3981.001 kb on + strand, within Pf6N2E2_3423 at 3981.001 kb on + strand, within Pf6N2E2_3423 at 3981.001 kb on + strand, within Pf6N2E2_3423 at 3981.001 kb on + strand, within Pf6N2E2_3423 at 3981.002 kb on - strand, within Pf6N2E2_3423 at 3981.002 kb on - strand, within Pf6N2E2_3423 at 3981.002 kb on - strand, within Pf6N2E2_3423 at 3981.002 kb on - strand, within Pf6N2E2_3423
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM putrescine remove 3,978,273 + -1.0 3,978,274 - +0.5 3,978,316 + +0.3 3,978,316 + -0.4 3,978,316 + -0.7 3,978,316 + +0.2 3,978,320 + +0.1 3,978,321 - +0.1 3,978,321 - +0.7 3,978,321 - +0.4 3,978,333 - -0.1 3,978,755 + Pf6N2E2_3421 0.53 +0.6 3,978,755 + Pf6N2E2_3421 0.53 +0.5 3,978,756 - Pf6N2E2_3421 0.53 -0.5 3,978,756 - Pf6N2E2_3421 0.53 +0.6 3,978,881 + Pf6N2E2_3421 0.70 +1.3 3,978,881 + Pf6N2E2_3421 0.70 +0.9 3,978,884 + Pf6N2E2_3421 0.70 -0.4 3,978,884 + Pf6N2E2_3421 0.70 -1.3 3,978,924 + Pf6N2E2_3421 0.75 +0.3 3,978,989 + Pf6N2E2_3421 0.84 -0.0 3,978,990 - Pf6N2E2_3421 0.84 +1.6 3,978,990 - Pf6N2E2_3421 0.84 +0.1 3,978,995 + Pf6N2E2_3421 0.85 -0.5 3,978,995 + Pf6N2E2_3421 0.85 +0.9 3,978,995 + Pf6N2E2_3421 0.85 +0.3 3,978,996 - Pf6N2E2_3421 0.85 -0.7 3,979,154 + +0.3 3,979,154 + -0.7 3,979,194 + +0.3 3,979,194 + -0.5 3,979,194 + +0.3 3,979,195 - +0.0 3,979,195 - +0.3 3,979,195 - +0.3 3,979,206 + +0.3 3,979,206 + +1.3 3,979,206 + -0.3 3,979,206 + -0.1 3,979,206 - +0.1 3,979,207 - +0.4 3,979,211 + +0.5 3,979,211 + +0.3 3,979,212 - +0.0 3,979,221 + +0.5 3,979,234 - +1.0 3,979,240 + -0.7 3,979,241 - +1.3 3,979,340 - +1.3 3,979,406 - Pf6N2E2_3422 0.15 -0.5 3,979,406 - Pf6N2E2_3422 0.15 +1.3 3,979,494 + Pf6N2E2_3422 0.25 -0.1 3,979,538 + Pf6N2E2_3422 0.30 -0.4 3,979,538 + Pf6N2E2_3422 0.30 +1.5 3,979,618 + Pf6N2E2_3422 0.39 +0.8 3,979,619 - Pf6N2E2_3422 0.39 +0.5 3,979,666 - Pf6N2E2_3422 0.44 +1.6 3,979,678 - Pf6N2E2_3422 0.46 +0.8 3,979,678 - Pf6N2E2_3422 0.46 -0.3 3,979,786 + Pf6N2E2_3422 0.58 +0.3 3,979,787 - Pf6N2E2_3422 0.58 -0.5 3,979,813 + Pf6N2E2_3422 0.61 +1.1 3,979,814 - Pf6N2E2_3422 0.61 +0.3 3,979,814 - Pf6N2E2_3422 0.61 -0.1 3,979,814 - Pf6N2E2_3422 0.61 +2.3 3,979,814 - Pf6N2E2_3422 0.61 -0.2 3,979,888 + Pf6N2E2_3422 0.69 +0.3 3,979,888 + Pf6N2E2_3422 0.69 +1.3 3,979,888 + Pf6N2E2_3422 0.69 -0.3 3,979,889 - Pf6N2E2_3422 0.69 -0.5 3,979,924 - Pf6N2E2_3422 0.73 +1.2 3,979,989 + Pf6N2E2_3422 0.81 +0.9 3,979,990 - Pf6N2E2_3422 0.81 +0.3 3,980,057 + Pf6N2E2_3422 0.88 +0.3 3,980,057 + Pf6N2E2_3422 0.88 +0.0 3,980,058 - Pf6N2E2_3422 0.88 -0.0 3,980,058 - Pf6N2E2_3422 0.88 +0.4 3,980,058 - Pf6N2E2_3422 0.88 +0.3 3,980,116 + -0.2 3,980,117 - +1.4 3,980,159 - -0.3 3,980,189 + +0.3 3,980,190 - +0.3 3,980,205 - -0.4 3,980,210 + +0.7 3,980,210 + +0.3 3,980,211 - -1.1 3,980,211 - +0.5 3,980,339 - Pf6N2E2_3423 0.18 -0.5 3,980,368 + Pf6N2E2_3423 0.22 -0.0 3,980,368 + Pf6N2E2_3423 0.22 -0.7 3,980,369 - Pf6N2E2_3423 0.22 +0.1 3,980,369 - Pf6N2E2_3423 0.22 +0.3 3,980,374 + Pf6N2E2_3423 0.22 +0.3 3,980,374 + Pf6N2E2_3423 0.22 +0.1 3,980,374 + Pf6N2E2_3423 0.22 +0.4 3,980,375 - Pf6N2E2_3423 0.22 -0.5 3,980,375 - Pf6N2E2_3423 0.22 +0.3 3,980,375 - Pf6N2E2_3423 0.22 +0.0 3,980,422 + Pf6N2E2_3423 0.27 +0.1 3,980,423 - Pf6N2E2_3423 0.27 +0.3 3,980,451 + Pf6N2E2_3423 0.30 -0.7 3,980,790 - Pf6N2E2_3423 0.66 -1.4 3,980,790 - Pf6N2E2_3423 0.66 -0.4 3,980,790 - Pf6N2E2_3423 0.66 +0.1 3,980,844 - Pf6N2E2_3423 0.72 -0.4 3,980,978 + Pf6N2E2_3423 0.86 +0.4 3,980,992 - Pf6N2E2_3423 0.87 -0.3 3,981,001 + Pf6N2E2_3423 0.88 -1.3 3,981,001 + Pf6N2E2_3423 0.88 -0.5 3,981,001 + Pf6N2E2_3423 0.88 -0.2 3,981,001 + Pf6N2E2_3423 0.88 -1.3 3,981,002 - Pf6N2E2_3423 0.88 +0.3 3,981,002 - Pf6N2E2_3423 0.88 +0.7 3,981,002 - Pf6N2E2_3423 0.88 +0.3 3,981,002 - Pf6N2E2_3423 0.88 -0.8
Or see this region's nucleotide sequence