Experiment: 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1431 and Pf6N2E2_1432 are separated by 11 nucleotides Pf6N2E2_1432 and Pf6N2E2_1433 are separated by 67 nucleotides Pf6N2E2_1433 and Pf6N2E2_1434 are separated by 13 nucleotides
Pf6N2E2_1431 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 1,680,698 to 1,681,765
_1431
Pf6N2E2_1432 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 1,681,777 to 1,682,658
_1432
Pf6N2E2_1433 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 1,682,726 to 1,683,508
_1433
Pf6N2E2_1434 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,683,522 to 1,684,682
_1434
Position (kb)
1682
1683
1684 Strain fitness (log2 ratio)
-2
-1
0
1 at 1681.779 kb on - strand at 1681.895 kb on + strand, within Pf6N2E2_1432 at 1681.896 kb on - strand, within Pf6N2E2_1432 at 1681.963 kb on - strand, within Pf6N2E2_1432 at 1682.351 kb on + strand, within Pf6N2E2_1432 at 1682.351 kb on + strand, within Pf6N2E2_1432 at 1682.352 kb on - strand, within Pf6N2E2_1432 at 1682.573 kb on + strand at 1682.606 kb on + strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.607 kb on - strand at 1682.638 kb on + strand at 1682.638 kb on + strand at 1682.911 kb on + strand, within Pf6N2E2_1433 at 1683.031 kb on - strand, within Pf6N2E2_1433 at 1683.233 kb on - strand, within Pf6N2E2_1433 at 1683.334 kb on - strand, within Pf6N2E2_1433 at 1683.466 kb on + strand at 1683.466 kb on + strand at 1683.467 kb on - strand at 1683.841 kb on + strand, within Pf6N2E2_1434 at 1683.860 kb on - strand, within Pf6N2E2_1434 at 1683.860 kb on - strand, within Pf6N2E2_1434 at 1684.262 kb on - strand, within Pf6N2E2_1434
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM putrescine remove 1,681,779 - +0.0 1,681,895 + Pf6N2E2_1432 0.13 -1.6 1,681,896 - Pf6N2E2_1432 0.13 +0.3 1,681,963 - Pf6N2E2_1432 0.21 +1.1 1,682,351 + Pf6N2E2_1432 0.65 -0.6 1,682,351 + Pf6N2E2_1432 0.65 +0.4 1,682,352 - Pf6N2E2_1432 0.65 -0.8 1,682,573 + +0.8 1,682,606 + -1.2 1,682,607 - -0.3 1,682,607 - -0.2 1,682,607 - +1.0 1,682,607 - -2.2 1,682,607 - +0.7 1,682,607 - +0.4 1,682,638 + +0.3 1,682,638 + -0.1 1,682,911 + Pf6N2E2_1433 0.24 -1.0 1,683,031 - Pf6N2E2_1433 0.39 -0.6 1,683,233 - Pf6N2E2_1433 0.65 -0.8 1,683,334 - Pf6N2E2_1433 0.78 -0.9 1,683,466 + +0.4 1,683,466 + -0.0 1,683,467 - -1.2 1,683,841 + Pf6N2E2_1434 0.27 -0.6 1,683,860 - Pf6N2E2_1434 0.29 -0.2 1,683,860 - Pf6N2E2_1434 0.29 +0.3 1,684,262 - Pf6N2E2_1434 0.64 +0.7
Or see this region's nucleotide sequence