Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1433

Experiment: 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1431 and Pf6N2E2_1432 are separated by 11 nucleotidesPf6N2E2_1432 and Pf6N2E2_1433 are separated by 67 nucleotidesPf6N2E2_1433 and Pf6N2E2_1434 are separated by 13 nucleotides Pf6N2E2_1431 - Branched-chain amino acid transport system permease protein LivM (TC 3.A.1.4.1), at 1,680,698 to 1,681,765 _1431 Pf6N2E2_1432 - High-affinity branched-chain amino acid transport system permease protein LivH (TC 3.A.1.4.1), at 1,681,777 to 1,682,658 _1432 Pf6N2E2_1433 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 1,682,726 to 1,683,508 _1433 Pf6N2E2_1434 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,683,522 to 1,684,682 _1434 Position (kb) 1682 1683 1684Strain fitness (log2 ratio) -2 -1 0 1at 1681.779 kb on - strandat 1681.895 kb on + strand, within Pf6N2E2_1432at 1681.896 kb on - strand, within Pf6N2E2_1432at 1681.963 kb on - strand, within Pf6N2E2_1432at 1682.351 kb on + strand, within Pf6N2E2_1432at 1682.351 kb on + strand, within Pf6N2E2_1432at 1682.352 kb on - strand, within Pf6N2E2_1432at 1682.573 kb on + strandat 1682.606 kb on + strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.607 kb on - strandat 1682.638 kb on + strandat 1682.638 kb on + strandat 1682.911 kb on + strand, within Pf6N2E2_1433at 1683.031 kb on - strand, within Pf6N2E2_1433at 1683.233 kb on - strand, within Pf6N2E2_1433at 1683.334 kb on - strand, within Pf6N2E2_1433at 1683.466 kb on + strandat 1683.466 kb on + strandat 1683.467 kb on - strandat 1683.841 kb on + strand, within Pf6N2E2_1434at 1683.860 kb on - strand, within Pf6N2E2_1434at 1683.860 kb on - strand, within Pf6N2E2_1434at 1684.262 kb on - strand, within Pf6N2E2_1434

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Per-strain Table

Position Strand Gene LocusTag Fraction 20 mM putrescine
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1,681,779 - +0.0
1,681,895 + Pf6N2E2_1432 0.13 -1.6
1,681,896 - Pf6N2E2_1432 0.13 +0.3
1,681,963 - Pf6N2E2_1432 0.21 +1.1
1,682,351 + Pf6N2E2_1432 0.65 -0.6
1,682,351 + Pf6N2E2_1432 0.65 +0.4
1,682,352 - Pf6N2E2_1432 0.65 -0.8
1,682,573 + +0.8
1,682,606 + -1.2
1,682,607 - -0.3
1,682,607 - -0.2
1,682,607 - +1.0
1,682,607 - -2.2
1,682,607 - +0.7
1,682,607 - +0.4
1,682,638 + +0.3
1,682,638 + -0.1
1,682,911 + Pf6N2E2_1433 0.24 -1.0
1,683,031 - Pf6N2E2_1433 0.39 -0.6
1,683,233 - Pf6N2E2_1433 0.65 -0.8
1,683,334 - Pf6N2E2_1433 0.78 -0.9
1,683,466 + +0.4
1,683,466 + -0.0
1,683,467 - -1.2
1,683,841 + Pf6N2E2_1434 0.27 -0.6
1,683,860 - Pf6N2E2_1434 0.29 -0.2
1,683,860 - Pf6N2E2_1434 0.29 +0.3
1,684,262 - Pf6N2E2_1434 0.64 +0.7

Or see this region's nucleotide sequence