Experiment: 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1043 and Pf6N2E2_1044 are separated by 176 nucleotides Pf6N2E2_1044 and Pf6N2E2_1045 are separated by 516 nucleotides
Pf6N2E2_1043 - Transcriptional regulator, IclR family, at 1,217,730 to 1,218,494
_1043
Pf6N2E2_1044 - hypothetical protein, at 1,218,671 to 1,218,862
_1044
Pf6N2E2_1045 - hypothetical protein, at 1,219,379 to 1,219,516
_1045
Position (kb)
1218
1219 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1217.710 kb on + strand at 1217.710 kb on + strand at 1217.710 kb on + strand at 1217.729 kb on + strand at 1217.730 kb on - strand at 1217.730 kb on - strand at 1217.883 kb on + strand, within Pf6N2E2_1043 at 1218.081 kb on + strand, within Pf6N2E2_1043 at 1218.081 kb on + strand, within Pf6N2E2_1043 at 1218.081 kb on + strand, within Pf6N2E2_1043 at 1218.082 kb on - strand, within Pf6N2E2_1043 at 1218.082 kb on - strand, within Pf6N2E2_1043 at 1218.320 kb on + strand, within Pf6N2E2_1043 at 1218.321 kb on - strand, within Pf6N2E2_1043 at 1218.387 kb on - strand, within Pf6N2E2_1043 at 1218.534 kb on + strand at 1218.534 kb on + strand at 1218.541 kb on - strand at 1218.541 kb on - strand at 1218.558 kb on + strand at 1218.559 kb on - strand at 1218.565 kb on + strand at 1218.566 kb on - strand at 1218.566 kb on - strand at 1218.566 kb on - strand at 1218.629 kb on + strand at 1218.629 kb on + strand at 1218.629 kb on + strand at 1218.633 kb on + strand at 1218.634 kb on - strand at 1218.673 kb on - strand at 1218.707 kb on + strand, within Pf6N2E2_1044 at 1218.708 kb on - strand, within Pf6N2E2_1044 at 1218.708 kb on - strand, within Pf6N2E2_1044 at 1218.708 kb on - strand, within Pf6N2E2_1044 at 1218.710 kb on - strand, within Pf6N2E2_1044 at 1218.808 kb on + strand, within Pf6N2E2_1044 at 1218.809 kb on - strand, within Pf6N2E2_1044 at 1218.868 kb on + strand at 1218.868 kb on + strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.869 kb on - strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.870 kb on + strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.871 kb on - strand at 1218.889 kb on - strand at 1218.950 kb on + strand at 1218.950 kb on + strand at 1218.950 kb on + strand at 1218.950 kb on + strand at 1218.950 kb on + strand at 1218.951 kb on - strand at 1218.951 kb on - strand at 1218.951 kb on - strand at 1219.027 kb on + strand at 1219.027 kb on + strand at 1219.179 kb on + strand at 1219.329 kb on - strand at 1219.514 kb on + strand at 1219.515 kb on - strand at 1219.515 kb on - strand at 1219.515 kb on - strand at 1219.562 kb on - strand at 1219.572 kb on + strand at 1219.573 kb on - strand at 1219.587 kb on - strand at 1219.640 kb on + strand at 1219.641 kb on - strand at 1219.641 kb on - strand at 1219.658 kb on + strand at 1219.715 kb on + strand at 1219.782 kb on + strand at 1219.783 kb on - strand at 1219.783 kb on - strand at 1219.789 kb on + strand at 1219.789 kb on + strand at 1219.790 kb on - strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.819 kb on + strand at 1219.820 kb on - strand at 1219.820 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 20 mM putrescine remove 1,217,710 + +0.4 1,217,710 + -0.6 1,217,710 + +2.4 1,217,729 + -1.3 1,217,730 - -0.6 1,217,730 - -0.8 1,217,883 + Pf6N2E2_1043 0.20 -0.6 1,218,081 + Pf6N2E2_1043 0.46 +1.0 1,218,081 + Pf6N2E2_1043 0.46 +0.2 1,218,081 + Pf6N2E2_1043 0.46 -2.0 1,218,082 - Pf6N2E2_1043 0.46 +1.4 1,218,082 - Pf6N2E2_1043 0.46 -0.2 1,218,320 + Pf6N2E2_1043 0.77 -0.6 1,218,321 - Pf6N2E2_1043 0.77 -0.6 1,218,387 - Pf6N2E2_1043 0.86 +0.4 1,218,534 + +0.2 1,218,534 + +0.3 1,218,541 - -1.6 1,218,541 - -1.0 1,218,558 + -0.2 1,218,559 - +0.4 1,218,565 + -1.6 1,218,566 - -1.6 1,218,566 - -0.9 1,218,566 - -0.3 1,218,629 + -0.6 1,218,629 + -0.2 1,218,629 + -0.8 1,218,633 + -1.6 1,218,634 - +1.0 1,218,673 - -0.4 1,218,707 + Pf6N2E2_1044 0.19 -0.6 1,218,708 - Pf6N2E2_1044 0.19 -1.0 1,218,708 - Pf6N2E2_1044 0.19 -0.6 1,218,708 - Pf6N2E2_1044 0.19 -1.6 1,218,710 - Pf6N2E2_1044 0.20 -1.2 1,218,808 + Pf6N2E2_1044 0.71 +0.4 1,218,809 - Pf6N2E2_1044 0.72 -0.6 1,218,868 + +0.2 1,218,868 + -1.6 1,218,869 - -0.6 1,218,869 - +1.2 1,218,869 - -0.6 1,218,869 - -0.1 1,218,869 - -0.6 1,218,869 - -0.2 1,218,869 - -1.6 1,218,869 - -0.0 1,218,869 - -0.6 1,218,869 - -0.6 1,218,869 - -1.1 1,218,870 + -0.6 1,218,870 + -0.2 1,218,870 + -0.0 1,218,870 + +1.4 1,218,870 + -0.6 1,218,870 + -0.0 1,218,870 + -1.6 1,218,871 - -0.6 1,218,871 - -0.0 1,218,871 - -0.6 1,218,871 - -1.3 1,218,871 - -0.3 1,218,871 - +0.2 1,218,871 - +1.4 1,218,871 - +1.2 1,218,871 - +2.0 1,218,889 - -1.6 1,218,950 + +0.8 1,218,950 + +0.4 1,218,950 + -0.2 1,218,950 + +0.4 1,218,950 + +2.4 1,218,951 - -0.3 1,218,951 - +1.0 1,218,951 - -0.6 1,219,027 + -0.6 1,219,027 + -0.3 1,219,179 + +0.1 1,219,329 - +0.2 1,219,514 + +1.4 1,219,515 - +0.4 1,219,515 - +0.0 1,219,515 - -2.2 1,219,562 - -0.0 1,219,572 + -0.2 1,219,573 - +0.0 1,219,587 - -0.3 1,219,640 + -0.6 1,219,641 - -0.6 1,219,641 - -0.6 1,219,658 + -1.6 1,219,715 + -0.6 1,219,782 + +0.3 1,219,783 - +0.4 1,219,783 - -0.6 1,219,789 + -1.6 1,219,789 + -0.6 1,219,790 - -0.6 1,219,819 + -1.0 1,219,819 + -0.6 1,219,819 + -1.6 1,219,819 + +1.3 1,219,820 - -1.6 1,219,820 - +0.2
Or see this region's nucleotide sequence