Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_935 and Pf6N2E2_936 are separated by 102 nucleotides Pf6N2E2_936 and Pf6N2E2_937 are separated by 79 nucleotides
Pf6N2E2_935 - Aldehyde dehydrogenase (EC 1.2.1.3), at 1,101,750 to 1,103,156
_935
Pf6N2E2_936 - NADH:flavin oxidoreductase/NADH oxidase, at 1,103,259 to 1,104,380
_936
Pf6N2E2_937 - Short-chain dehydrodenase (gene dltE), at 1,104,460 to 1,105,221
_937
Position (kb)
1103
1104
1105 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1102.271 kb on + strand, within Pf6N2E2_935 at 1102.271 kb on + strand, within Pf6N2E2_935 at 1102.271 kb on + strand, within Pf6N2E2_935 at 1102.272 kb on - strand, within Pf6N2E2_935 at 1102.272 kb on - strand, within Pf6N2E2_935 at 1102.272 kb on - strand, within Pf6N2E2_935 at 1102.272 kb on - strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.278 kb on - strand, within Pf6N2E2_935 at 1102.520 kb on + strand, within Pf6N2E2_935 at 1102.530 kb on + strand, within Pf6N2E2_935 at 1102.530 kb on + strand, within Pf6N2E2_935 at 1102.531 kb on - strand, within Pf6N2E2_935 at 1102.562 kb on + strand, within Pf6N2E2_935 at 1102.671 kb on + strand, within Pf6N2E2_935 at 1102.762 kb on - strand, within Pf6N2E2_935 at 1102.808 kb on + strand, within Pf6N2E2_935 at 1102.809 kb on - strand, within Pf6N2E2_935 at 1102.815 kb on + strand, within Pf6N2E2_935 at 1102.893 kb on + strand, within Pf6N2E2_935 at 1102.893 kb on + strand, within Pf6N2E2_935 at 1102.893 kb on + strand, within Pf6N2E2_935 at 1102.999 kb on + strand, within Pf6N2E2_935 at 1103.001 kb on + strand, within Pf6N2E2_935 at 1103.002 kb on - strand, within Pf6N2E2_935 at 1103.123 kb on - strand at 1103.133 kb on + strand at 1103.133 kb on + strand at 1103.134 kb on - strand at 1103.167 kb on + strand at 1103.168 kb on - strand at 1103.168 kb on - strand at 1103.260 kb on + strand at 1103.261 kb on - strand at 1103.278 kb on + strand at 1103.287 kb on + strand at 1103.287 kb on + strand at 1103.287 kb on + strand at 1103.288 kb on - strand at 1103.288 kb on - strand at 1103.308 kb on + strand at 1103.309 kb on - strand at 1103.309 kb on - strand at 1103.311 kb on + strand at 1103.312 kb on - strand at 1103.312 kb on - strand at 1103.382 kb on - strand, within Pf6N2E2_936 at 1103.382 kb on - strand, within Pf6N2E2_936 at 1103.595 kb on - strand, within Pf6N2E2_936 at 1103.629 kb on + strand, within Pf6N2E2_936 at 1103.629 kb on + strand, within Pf6N2E2_936 at 1103.650 kb on + strand, within Pf6N2E2_936 at 1103.687 kb on + strand, within Pf6N2E2_936 at 1103.877 kb on + strand, within Pf6N2E2_936 at 1103.989 kb on + strand, within Pf6N2E2_936 at 1103.990 kb on - strand, within Pf6N2E2_936 at 1103.995 kb on + strand, within Pf6N2E2_936 at 1103.995 kb on + strand, within Pf6N2E2_936 at 1104.190 kb on + strand, within Pf6N2E2_936 at 1104.190 kb on + strand, within Pf6N2E2_936 at 1104.212 kb on + strand, within Pf6N2E2_936 at 1104.212 kb on + strand, within Pf6N2E2_936 at 1104.231 kb on + strand, within Pf6N2E2_936 at 1104.231 kb on + strand, within Pf6N2E2_936 at 1104.231 kb on + strand, within Pf6N2E2_936 at 1104.231 kb on + strand, within Pf6N2E2_936 at 1104.232 kb on - strand, within Pf6N2E2_936 at 1104.232 kb on - strand, within Pf6N2E2_936 at 1104.259 kb on + strand, within Pf6N2E2_936 at 1104.263 kb on - strand, within Pf6N2E2_936 at 1104.503 kb on + strand at 1104.504 kb on - strand at 1104.504 kb on - strand at 1104.537 kb on + strand, within Pf6N2E2_937 at 1104.537 kb on + strand, within Pf6N2E2_937 at 1104.538 kb on - strand, within Pf6N2E2_937 at 1104.653 kb on + strand, within Pf6N2E2_937 at 1104.827 kb on + strand, within Pf6N2E2_937 at 1104.827 kb on + strand, within Pf6N2E2_937 at 1104.827 kb on + strand, within Pf6N2E2_937 at 1104.828 kb on - strand at 1104.908 kb on + strand, within Pf6N2E2_937 at 1104.999 kb on + strand, within Pf6N2E2_937 at 1104.999 kb on + strand, within Pf6N2E2_937 at 1104.999 kb on + strand, within Pf6N2E2_937 at 1104.999 kb on + strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.000 kb on - strand, within Pf6N2E2_937 at 1105.066 kb on + strand, within Pf6N2E2_937 at 1105.066 kb on + strand, within Pf6N2E2_937 at 1105.066 kb on + strand, within Pf6N2E2_937 at 1105.067 kb on - strand, within Pf6N2E2_937 at 1105.067 kb on - strand, within Pf6N2E2_937 at 1105.122 kb on + strand, within Pf6N2E2_937 at 1105.127 kb on - strand, within Pf6N2E2_937 at 1105.199 kb on - strand at 1105.304 kb on + strand at 1105.305 kb on - strand at 1105.305 kb on - strand at 1105.328 kb on - strand at 1105.328 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,102,271 + Pf6N2E2_935 0.37 -0.2 1,102,271 + Pf6N2E2_935 0.37 +0.2 1,102,271 + Pf6N2E2_935 0.37 -0.6 1,102,272 - Pf6N2E2_935 0.37 -0.7 1,102,272 - Pf6N2E2_935 0.37 +0.1 1,102,272 - Pf6N2E2_935 0.37 -0.3 1,102,272 - Pf6N2E2_935 0.37 -0.9 1,102,275 + Pf6N2E2_935 0.37 +0.8 1,102,275 + Pf6N2E2_935 0.37 +1.4 1,102,275 + Pf6N2E2_935 0.37 -0.6 1,102,275 + Pf6N2E2_935 0.37 -0.2 1,102,275 + Pf6N2E2_935 0.37 -1.1 1,102,275 + Pf6N2E2_935 0.37 +1.1 1,102,275 + Pf6N2E2_935 0.37 -1.0 1,102,275 + Pf6N2E2_935 0.37 +0.3 1,102,276 - Pf6N2E2_935 0.37 +0.4 1,102,276 - Pf6N2E2_935 0.37 +0.7 1,102,276 - Pf6N2E2_935 0.37 +0.1 1,102,276 - Pf6N2E2_935 0.37 +0.6 1,102,276 - Pf6N2E2_935 0.37 +0.2 1,102,276 - Pf6N2E2_935 0.37 -0.1 1,102,276 - Pf6N2E2_935 0.37 +0.7 1,102,276 - Pf6N2E2_935 0.37 -0.1 1,102,276 - Pf6N2E2_935 0.37 -0.8 1,102,278 - Pf6N2E2_935 0.38 -0.1 1,102,520 + Pf6N2E2_935 0.55 +0.7 1,102,530 + Pf6N2E2_935 0.55 -0.6 1,102,530 + Pf6N2E2_935 0.55 +0.6 1,102,531 - Pf6N2E2_935 0.56 +0.5 1,102,562 + Pf6N2E2_935 0.58 -0.9 1,102,671 + Pf6N2E2_935 0.65 +0.4 1,102,762 - Pf6N2E2_935 0.72 +0.5 1,102,808 + Pf6N2E2_935 0.75 +0.7 1,102,809 - Pf6N2E2_935 0.75 +0.4 1,102,815 + Pf6N2E2_935 0.76 +1.0 1,102,893 + Pf6N2E2_935 0.81 -0.1 1,102,893 + Pf6N2E2_935 0.81 +1.0 1,102,893 + Pf6N2E2_935 0.81 -0.0 1,102,999 + Pf6N2E2_935 0.89 -0.1 1,103,001 + Pf6N2E2_935 0.89 +0.2 1,103,002 - Pf6N2E2_935 0.89 -0.3 1,103,123 - -0.6 1,103,133 + -0.2 1,103,133 + +0.8 1,103,134 - -0.6 1,103,167 + +0.0 1,103,168 - -0.5 1,103,168 - +0.3 1,103,260 + +0.8 1,103,261 - -0.2 1,103,278 + -0.3 1,103,287 + -1.1 1,103,287 + +1.0 1,103,287 + +2.3 1,103,288 - -1.6 1,103,288 - -0.6 1,103,308 + -0.1 1,103,309 - -0.6 1,103,309 - -1.1 1,103,311 + -0.6 1,103,312 - +0.9 1,103,312 - -1.0 1,103,382 - Pf6N2E2_936 0.11 -0.1 1,103,382 - Pf6N2E2_936 0.11 -0.6 1,103,595 - Pf6N2E2_936 0.30 -0.1 1,103,629 + Pf6N2E2_936 0.33 +0.3 1,103,629 + Pf6N2E2_936 0.33 -1.0 1,103,650 + Pf6N2E2_936 0.35 +0.2 1,103,687 + Pf6N2E2_936 0.38 -0.6 1,103,877 + Pf6N2E2_936 0.55 -0.0 1,103,989 + Pf6N2E2_936 0.65 -2.1 1,103,990 - Pf6N2E2_936 0.65 -1.1 1,103,995 + Pf6N2E2_936 0.66 -0.6 1,103,995 + Pf6N2E2_936 0.66 +0.4 1,104,190 + Pf6N2E2_936 0.83 -1.0 1,104,190 + Pf6N2E2_936 0.83 -0.8 1,104,212 + Pf6N2E2_936 0.85 -0.6 1,104,212 + Pf6N2E2_936 0.85 -0.6 1,104,231 + Pf6N2E2_936 0.87 -0.7 1,104,231 + Pf6N2E2_936 0.87 -0.6 1,104,231 + Pf6N2E2_936 0.87 +0.4 1,104,231 + Pf6N2E2_936 0.87 -0.1 1,104,232 - Pf6N2E2_936 0.87 +0.4 1,104,232 - Pf6N2E2_936 0.87 -0.6 1,104,259 + Pf6N2E2_936 0.89 +0.0 1,104,263 - Pf6N2E2_936 0.89 -0.3 1,104,503 + -0.6 1,104,504 - -1.1 1,104,504 - -1.6 1,104,537 + Pf6N2E2_937 0.10 -0.6 1,104,537 + Pf6N2E2_937 0.10 +0.4 1,104,538 - Pf6N2E2_937 0.10 -0.6 1,104,653 + Pf6N2E2_937 0.25 -0.3 1,104,827 + Pf6N2E2_937 0.48 -0.6 1,104,827 + Pf6N2E2_937 0.48 -0.6 1,104,827 + Pf6N2E2_937 0.48 +0.9 1,104,828 - +2.0 1,104,908 + Pf6N2E2_937 0.59 -0.6 1,104,999 + Pf6N2E2_937 0.71 -0.1 1,104,999 + Pf6N2E2_937 0.71 +1.1 1,104,999 + Pf6N2E2_937 0.71 +0.0 1,104,999 + Pf6N2E2_937 0.71 -0.3 1,105,000 - Pf6N2E2_937 0.71 +0.4 1,105,000 - Pf6N2E2_937 0.71 +0.3 1,105,000 - Pf6N2E2_937 0.71 +0.0 1,105,000 - Pf6N2E2_937 0.71 -0.0 1,105,000 - Pf6N2E2_937 0.71 -0.6 1,105,000 - Pf6N2E2_937 0.71 -1.4 1,105,000 - Pf6N2E2_937 0.71 -0.6 1,105,000 - Pf6N2E2_937 0.71 -0.1 1,105,000 - Pf6N2E2_937 0.71 +0.8 1,105,066 + Pf6N2E2_937 0.80 -0.6 1,105,066 + Pf6N2E2_937 0.80 +0.9 1,105,066 + Pf6N2E2_937 0.80 +0.4 1,105,067 - Pf6N2E2_937 0.80 +0.0 1,105,067 - Pf6N2E2_937 0.80 +0.4 1,105,122 + Pf6N2E2_937 0.87 -0.2 1,105,127 - Pf6N2E2_937 0.88 +0.2 1,105,199 - +0.4 1,105,304 + -0.6 1,105,305 - -1.6 1,105,305 - -0.6 1,105,328 - -0.6 1,105,328 - -0.6
Or see this region's nucleotide sequence