Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_357 and Pf6N2E2_358 are separated by 21 nucleotides Pf6N2E2_358 and Pf6N2E2_359 are separated by 36 nucleotides Pf6N2E2_359 and Pf6N2E2_360 are separated by 86 nucleotides
Pf6N2E2_357 - hypothetical protein, at 422,979 to 423,353
_357
Pf6N2E2_358 - Haloacid dehalogenase, type II, at 423,375 to 424,043
_358
Pf6N2E2_359 - Opine oxidase subunit B, at 424,080 to 425,354
_359
Pf6N2E2_360 - ABC peptide transporter, periplasmic ligand binding protein, at 425,441 to 427,084
_360
Position (kb) 424
425
426 Strain fitness (log2 ratio) -1
0
1 at 423.137 kb on + strand, within Pf6N2E2_357 at 423.137 kb on + strand, within Pf6N2E2_357 at 423.138 kb on - strand, within Pf6N2E2_357 at 423.138 kb on - strand, within Pf6N2E2_357 at 423.282 kb on + strand, within Pf6N2E2_357 at 423.282 kb on + strand, within Pf6N2E2_357 at 423.283 kb on - strand, within Pf6N2E2_357 at 423.283 kb on - strand, within Pf6N2E2_357 at 423.396 kb on + strand at 423.396 kb on + strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.397 kb on - strand at 423.556 kb on - strand, within Pf6N2E2_358 at 423.633 kb on + strand, within Pf6N2E2_358 at 423.633 kb on + strand, within Pf6N2E2_358 at 423.781 kb on - strand, within Pf6N2E2_358 at 423.813 kb on + strand, within Pf6N2E2_358 at 424.095 kb on + strand at 424.360 kb on - strand, within Pf6N2E2_359 at 424.363 kb on - strand, within Pf6N2E2_359 at 424.371 kb on + strand, within Pf6N2E2_359 at 424.477 kb on - strand at 424.861 kb on + strand, within Pf6N2E2_359 at 424.862 kb on - strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.908 kb on + strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.909 kb on - strand, within Pf6N2E2_359 at 424.935 kb on + strand, within Pf6N2E2_359 at 424.936 kb on - strand, within Pf6N2E2_359 at 425.085 kb on + strand, within Pf6N2E2_359 at 425.085 kb on + strand, within Pf6N2E2_359 at 425.325 kb on + strand at 425.328 kb on + strand at 425.344 kb on - strand at 425.414 kb on + strand at 425.432 kb on - strand at 425.735 kb on + strand, within Pf6N2E2_360 at 425.736 kb on - strand, within Pf6N2E2_360 at 425.736 kb on - strand, within Pf6N2E2_360 at 425.747 kb on + strand, within Pf6N2E2_360 at 425.747 kb on + strand, within Pf6N2E2_360 at 425.747 kb on + strand, within Pf6N2E2_360 at 425.747 kb on + strand, within Pf6N2E2_360 at 425.748 kb on - strand, within Pf6N2E2_360 at 425.748 kb on - strand, within Pf6N2E2_360 at 425.756 kb on + strand, within Pf6N2E2_360 at 425.757 kb on - strand, within Pf6N2E2_360 at 426.013 kb on - strand, within Pf6N2E2_360 at 426.013 kb on - strand, within Pf6N2E2_360 at 426.013 kb on - strand, within Pf6N2E2_360 at 426.172 kb on + strand, within Pf6N2E2_360 at 426.180 kb on - strand, within Pf6N2E2_360 at 426.180 kb on - strand, within Pf6N2E2_360 at 426.180 kb on - strand, within Pf6N2E2_360 at 426.201 kb on - strand, within Pf6N2E2_360
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 423,137 + Pf6N2E2_357 0.42 -1.3 423,137 + Pf6N2E2_357 0.42 -0.1 423,138 - Pf6N2E2_357 0.42 +0.4 423,138 - Pf6N2E2_357 0.42 -0.6 423,282 + Pf6N2E2_357 0.81 +0.1 423,282 + Pf6N2E2_357 0.81 +1.6 423,283 - Pf6N2E2_357 0.81 -0.9 423,283 - Pf6N2E2_357 0.81 +0.2 423,396 + +0.9 423,396 + -0.4 423,397 - -0.9 423,397 - +1.5 423,397 - +0.5 423,397 - +0.1 423,397 - -0.6 423,397 - +1.1 423,556 - Pf6N2E2_358 0.27 +0.7 423,633 + Pf6N2E2_358 0.39 +1.3 423,633 + Pf6N2E2_358 0.39 +0.9 423,781 - Pf6N2E2_358 0.61 +0.2 423,813 + Pf6N2E2_358 0.65 -0.3 424,095 + -0.3 424,360 - Pf6N2E2_359 0.22 -0.0 424,363 - Pf6N2E2_359 0.22 +1.7 424,371 + Pf6N2E2_359 0.23 +1.7 424,477 - -0.3 424,861 + Pf6N2E2_359 0.61 +1.7 424,862 - Pf6N2E2_359 0.61 -1.0 424,908 + Pf6N2E2_359 0.65 -0.5 424,908 + Pf6N2E2_359 0.65 +0.4 424,908 + Pf6N2E2_359 0.65 -1.3 424,908 + Pf6N2E2_359 0.65 -0.1 424,908 + Pf6N2E2_359 0.65 -0.5 424,908 + Pf6N2E2_359 0.65 -0.4 424,908 + Pf6N2E2_359 0.65 +0.6 424,908 + Pf6N2E2_359 0.65 +1.0 424,908 + Pf6N2E2_359 0.65 +0.6 424,909 - Pf6N2E2_359 0.65 -0.5 424,909 - Pf6N2E2_359 0.65 -0.3 424,909 - Pf6N2E2_359 0.65 -0.6 424,909 - Pf6N2E2_359 0.65 -0.5 424,909 - Pf6N2E2_359 0.65 -1.6 424,909 - Pf6N2E2_359 0.65 -0.2 424,909 - Pf6N2E2_359 0.65 -1.2 424,935 + Pf6N2E2_359 0.67 -0.3 424,936 - Pf6N2E2_359 0.67 +0.7 425,085 + Pf6N2E2_359 0.79 -1.2 425,085 + Pf6N2E2_359 0.79 -0.7 425,325 + -0.7 425,328 + +0.3 425,344 - -0.9 425,414 + -0.9 425,432 - +0.7 425,735 + Pf6N2E2_360 0.18 +0.7 425,736 - Pf6N2E2_360 0.18 -0.3 425,736 - Pf6N2E2_360 0.18 -0.0 425,747 + Pf6N2E2_360 0.19 -1.3 425,747 + Pf6N2E2_360 0.19 -0.3 425,747 + Pf6N2E2_360 0.19 -0.9 425,747 + Pf6N2E2_360 0.19 -0.1 425,748 - Pf6N2E2_360 0.19 +0.1 425,748 - Pf6N2E2_360 0.19 +0.7 425,756 + Pf6N2E2_360 0.19 +0.2 425,757 - Pf6N2E2_360 0.19 +0.1 426,013 - Pf6N2E2_360 0.35 -1.0 426,013 - Pf6N2E2_360 0.35 -0.0 426,013 - Pf6N2E2_360 0.35 -0.3 426,172 + Pf6N2E2_360 0.44 +0.6 426,180 - Pf6N2E2_360 0.45 +0.3 426,180 - Pf6N2E2_360 0.45 -0.0 426,180 - Pf6N2E2_360 0.45 -0.3 426,201 - Pf6N2E2_360 0.46 -0.3
Or see this region's nucleotide sequence