Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_341 and Pf6N2E2_342 overlap by 4 nucleotides Pf6N2E2_342 and Pf6N2E2_343 are separated by 397 nucleotides
Pf6N2E2_341 - sensor histidine kinase, at 408,044 to 409,390
_341
Pf6N2E2_342 - DNA-binding response regulator, at 409,387 to 410,097
_342
Pf6N2E2_343 - VacJ-like lipoprotein precursor, at 410,495 to 411,226
_343
Position (kb) 409
410
411 Strain fitness (log2 ratio) -2
-1
0
1
2 at 408.668 kb on + strand, within Pf6N2E2_341 at 409.011 kb on + strand, within Pf6N2E2_341 at 409.012 kb on - strand, within Pf6N2E2_341 at 409.805 kb on + strand, within Pf6N2E2_342 at 410.086 kb on + strand at 410.087 kb on - strand at 410.087 kb on - strand at 410.087 kb on - strand at 410.120 kb on + strand at 410.121 kb on - strand at 410.135 kb on + strand at 410.135 kb on + strand at 410.135 kb on + strand at 410.136 kb on - strand at 410.160 kb on + strand at 410.161 kb on - strand at 410.162 kb on + strand at 410.162 kb on + strand at 410.162 kb on + strand at 410.163 kb on - strand at 410.178 kb on + strand at 410.179 kb on - strand at 410.404 kb on - strand at 410.437 kb on + strand at 410.490 kb on + strand at 410.490 kb on + strand at 410.491 kb on - strand at 410.491 kb on - strand at 410.491 kb on - strand at 410.561 kb on + strand at 410.561 kb on + strand at 410.561 kb on + strand at 410.562 kb on - strand at 410.562 kb on - strand at 410.630 kb on + strand, within Pf6N2E2_343 at 410.630 kb on + strand, within Pf6N2E2_343 at 410.630 kb on + strand, within Pf6N2E2_343 at 410.630 kb on + strand, within Pf6N2E2_343 at 410.630 kb on + strand, within Pf6N2E2_343 at 410.630 kb on + strand, within Pf6N2E2_343 at 410.631 kb on - strand, within Pf6N2E2_343 at 410.631 kb on - strand, within Pf6N2E2_343 at 410.631 kb on - strand, within Pf6N2E2_343 at 410.631 kb on - strand, within Pf6N2E2_343 at 410.631 kb on - strand, within Pf6N2E2_343 at 410.631 kb on - strand, within Pf6N2E2_343 at 410.631 kb on - strand, within Pf6N2E2_343 at 410.734 kb on - strand, within Pf6N2E2_343 at 410.745 kb on + strand, within Pf6N2E2_343 at 410.745 kb on + strand, within Pf6N2E2_343 at 410.745 kb on + strand, within Pf6N2E2_343 at 410.745 kb on + strand, within Pf6N2E2_343 at 410.746 kb on - strand, within Pf6N2E2_343 at 410.746 kb on - strand, within Pf6N2E2_343 at 410.746 kb on - strand, within Pf6N2E2_343 at 410.858 kb on - strand, within Pf6N2E2_343
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 408,668 + Pf6N2E2_341 0.46 +0.9 409,011 + Pf6N2E2_341 0.72 -1.0 409,012 - Pf6N2E2_341 0.72 +1.0 409,805 + Pf6N2E2_342 0.59 -1.3 410,086 + -0.0 410,087 - -0.8 410,087 - -1.1 410,087 - +0.0 410,120 + -0.0 410,121 - +0.5 410,135 + +1.4 410,135 + +1.3 410,135 + -0.3 410,136 - -0.3 410,160 + +1.3 410,161 - +1.3 410,162 + -0.2 410,162 + +2.0 410,162 + -0.3 410,163 - +0.6 410,178 + +0.5 410,179 - +0.7 410,404 - -0.4 410,437 + -0.7 410,490 + +0.7 410,490 + +0.1 410,491 - -1.3 410,491 - -1.5 410,491 - -0.9 410,561 + +0.1 410,561 + +1.0 410,561 + -0.6 410,562 - -0.3 410,562 - -0.0 410,630 + Pf6N2E2_343 0.18 -1.6 410,630 + Pf6N2E2_343 0.18 +0.4 410,630 + Pf6N2E2_343 0.18 +0.3 410,630 + Pf6N2E2_343 0.18 -0.3 410,630 + Pf6N2E2_343 0.18 -1.0 410,630 + Pf6N2E2_343 0.18 +0.4 410,631 - Pf6N2E2_343 0.19 -0.3 410,631 - Pf6N2E2_343 0.19 +1.0 410,631 - Pf6N2E2_343 0.19 -0.0 410,631 - Pf6N2E2_343 0.19 +0.4 410,631 - Pf6N2E2_343 0.19 -0.3 410,631 - Pf6N2E2_343 0.19 -0.1 410,631 - Pf6N2E2_343 0.19 +0.2 410,734 - Pf6N2E2_343 0.33 -1.2 410,745 + Pf6N2E2_343 0.34 -1.9 410,745 + Pf6N2E2_343 0.34 +0.8 410,745 + Pf6N2E2_343 0.34 +0.8 410,745 + Pf6N2E2_343 0.34 +1.3 410,746 - Pf6N2E2_343 0.34 +0.4 410,746 - Pf6N2E2_343 0.34 -0.3 410,746 - Pf6N2E2_343 0.34 -1.3 410,858 - Pf6N2E2_343 0.50 +0.5
Or see this region's nucleotide sequence