Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1843

Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1842 and Pf6N2E2_1843 are separated by 429 nucleotidesPf6N2E2_1843 and Pf6N2E2_1844 are separated by 201 nucleotidesPf6N2E2_1844 and Pf6N2E2_1845 are separated by 235 nucleotides Pf6N2E2_1842 - Transcriptional regulator, TetR family, at 2,179,706 to 2,180,350 _1842 Pf6N2E2_1843 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 2,180,780 to 2,181,541 _1843 Pf6N2E2_1844 - MaoC-related acyl dehydratase, at 2,181,743 to 2,182,228 _1844 Pf6N2E2_1845 - FIG005548: transport protein, at 2,182,464 to 2,184,959 _1845 Position (kb) 2180 2181 2182Strain fitness (log2 ratio) -2 -1 0 1at 2179.996 kb on - strand, within Pf6N2E2_1842at 2180.171 kb on - strand, within Pf6N2E2_1842at 2180.171 kb on - strand, within Pf6N2E2_1842at 2180.178 kb on - strand, within Pf6N2E2_1842at 2180.224 kb on + strand, within Pf6N2E2_1842at 2180.224 kb on + strand, within Pf6N2E2_1842at 2180.225 kb on - strand, within Pf6N2E2_1842at 2180.225 kb on - strand, within Pf6N2E2_1842at 2180.241 kb on + strand, within Pf6N2E2_1842at 2180.360 kb on - strandat 2180.678 kb on + strandat 2180.937 kb on + strand, within Pf6N2E2_1843at 2181.173 kb on + strand, within Pf6N2E2_1843at 2181.174 kb on - strand, within Pf6N2E2_1843at 2181.206 kb on + strand, within Pf6N2E2_1843at 2181.206 kb on + strand, within Pf6N2E2_1843at 2181.206 kb on + strand, within Pf6N2E2_1843at 2181.207 kb on - strand, within Pf6N2E2_1843at 2181.207 kb on - strand, within Pf6N2E2_1843at 2181.207 kb on - strand, within Pf6N2E2_1843at 2181.207 kb on - strand, within Pf6N2E2_1843at 2181.219 kb on - strand, within Pf6N2E2_1843at 2181.249 kb on - strand, within Pf6N2E2_1843at 2181.597 kb on + strandat 2181.598 kb on - strandat 2181.678 kb on + strandat 2181.716 kb on + strandat 2181.858 kb on - strand, within Pf6N2E2_1844at 2181.876 kb on - strand, within Pf6N2E2_1844at 2181.955 kb on - strand, within Pf6N2E2_1844at 2182.083 kb on + strand, within Pf6N2E2_1844at 2182.084 kb on - strand, within Pf6N2E2_1844at 2182.084 kb on - strand, within Pf6N2E2_1844at 2182.084 kb on - strand, within Pf6N2E2_1844at 2182.151 kb on - strand, within Pf6N2E2_1844at 2182.256 kb on - strandat 2182.325 kb on - strandat 2182.449 kb on + strandat 2182.450 kb on - strandat 2182.450 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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2,179,996 - Pf6N2E2_1842 0.45 -0.4
2,180,171 - Pf6N2E2_1842 0.72 -0.8
2,180,171 - Pf6N2E2_1842 0.72 -0.4
2,180,178 - Pf6N2E2_1842 0.73 -0.1
2,180,224 + Pf6N2E2_1842 0.80 -0.1
2,180,224 + Pf6N2E2_1842 0.80 +0.5
2,180,225 - Pf6N2E2_1842 0.80 -2.4
2,180,225 - Pf6N2E2_1842 0.80 +0.3
2,180,241 + Pf6N2E2_1842 0.83 +0.2
2,180,360 - +0.1
2,180,678 + -0.4
2,180,937 + Pf6N2E2_1843 0.21 -1.2
2,181,173 + Pf6N2E2_1843 0.52 -1.0
2,181,174 - Pf6N2E2_1843 0.52 -1.8
2,181,206 + Pf6N2E2_1843 0.56 -0.8
2,181,206 + Pf6N2E2_1843 0.56 -0.1
2,181,206 + Pf6N2E2_1843 0.56 +0.6
2,181,207 - Pf6N2E2_1843 0.56 -0.4
2,181,207 - Pf6N2E2_1843 0.56 -0.4
2,181,207 - Pf6N2E2_1843 0.56 -0.4
2,181,207 - Pf6N2E2_1843 0.56 -0.7
2,181,219 - Pf6N2E2_1843 0.58 -0.7
2,181,249 - Pf6N2E2_1843 0.62 +0.7
2,181,597 + -0.4
2,181,598 - +0.6
2,181,678 + +0.5
2,181,716 + -0.6
2,181,858 - Pf6N2E2_1844 0.24 -1.0
2,181,876 - Pf6N2E2_1844 0.27 +1.2
2,181,955 - Pf6N2E2_1844 0.44 +0.4
2,182,083 + Pf6N2E2_1844 0.70 -0.4
2,182,084 - Pf6N2E2_1844 0.70 +0.5
2,182,084 - Pf6N2E2_1844 0.70 -0.4
2,182,084 - Pf6N2E2_1844 0.70 +0.5
2,182,151 - Pf6N2E2_1844 0.84 -0.4
2,182,256 - +0.6
2,182,325 - +0.1
2,182,449 + -0.2
2,182,450 - +0.6
2,182,450 - +0.5

Or see this region's nucleotide sequence