Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1710

Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1709 and Pf6N2E2_1710 are separated by 43 nucleotidesPf6N2E2_1710 and Pf6N2E2_1711 are separated by 116 nucleotidesPf6N2E2_1711 and Pf6N2E2_1712 overlap by 14 nucleotides Pf6N2E2_1709 - multidrug resistance transporter, Bcr/CflA family, at 2,017,318 to 2,018,538 _1709 Pf6N2E2_1710 - FIG00953587: hypothetical protein, at 2,018,582 to 2,019,856 _1710 Pf6N2E2_1711 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 2,019,973 to 2,020,857 _1711 Pf6N2E2_1712 - Permease of the drug/metabolite transporter (DMT) superfamily, at 2,020,844 to 2,021,749 _1712 Position (kb) 2018 2019 2020Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2017.904 kb on + strand, within Pf6N2E2_1709at 2017.904 kb on + strand, within Pf6N2E2_1709at 2017.976 kb on - strand, within Pf6N2E2_1709at 2018.120 kb on - strand, within Pf6N2E2_1709at 2018.120 kb on - strand, within Pf6N2E2_1709at 2018.281 kb on + strand, within Pf6N2E2_1709at 2018.281 kb on + strand, within Pf6N2E2_1709at 2018.675 kb on + strandat 2018.676 kb on - strandat 2018.752 kb on - strand, within Pf6N2E2_1710at 2019.029 kb on + strand, within Pf6N2E2_1710at 2019.029 kb on + strand, within Pf6N2E2_1710at 2019.029 kb on + strand, within Pf6N2E2_1710at 2019.029 kb on + strand, within Pf6N2E2_1710at 2019.030 kb on - strand, within Pf6N2E2_1710at 2019.030 kb on - strand, within Pf6N2E2_1710at 2019.030 kb on - strand, within Pf6N2E2_1710at 2019.030 kb on - strand, within Pf6N2E2_1710at 2019.030 kb on - strand, within Pf6N2E2_1710at 2019.030 kb on - strand, within Pf6N2E2_1710at 2019.030 kb on - strand, within Pf6N2E2_1710at 2019.030 kb on - strand, within Pf6N2E2_1710at 2019.031 kb on + strand, within Pf6N2E2_1710at 2019.105 kb on + strand, within Pf6N2E2_1710at 2019.106 kb on - strand, within Pf6N2E2_1710at 2019.123 kb on + strand, within Pf6N2E2_1710at 2019.363 kb on + strand, within Pf6N2E2_1710at 2019.364 kb on - strand, within Pf6N2E2_1710at 2019.364 kb on - strand, within Pf6N2E2_1710at 2019.442 kb on - strand, within Pf6N2E2_1710at 2019.576 kb on + strand, within Pf6N2E2_1710at 2019.577 kb on - strand, within Pf6N2E2_1710at 2019.751 kb on - strandat 2019.839 kb on - strandat 2019.847 kb on + strandat 2019.847 kb on + strandat 2020.037 kb on + strandat 2020.123 kb on + strand, within Pf6N2E2_1711at 2020.123 kb on + strand, within Pf6N2E2_1711at 2020.124 kb on - strand, within Pf6N2E2_1711at 2020.399 kb on + strand, within Pf6N2E2_1711at 2020.472 kb on + strand, within Pf6N2E2_1711at 2020.473 kb on - strand, within Pf6N2E2_1711at 2020.473 kb on - strand, within Pf6N2E2_1711

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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2,017,904 + Pf6N2E2_1709 0.48 -1.1
2,017,904 + Pf6N2E2_1709 0.48 +0.1
2,017,976 - Pf6N2E2_1709 0.54 +0.2
2,018,120 - Pf6N2E2_1709 0.66 -0.1
2,018,120 - Pf6N2E2_1709 0.66 +0.7
2,018,281 + Pf6N2E2_1709 0.79 +0.2
2,018,281 + Pf6N2E2_1709 0.79 +2.7
2,018,675 + -2.1
2,018,676 - +0.0
2,018,752 - Pf6N2E2_1710 0.13 +0.5
2,019,029 + Pf6N2E2_1710 0.35 +1.5
2,019,029 + Pf6N2E2_1710 0.35 +1.5
2,019,029 + Pf6N2E2_1710 0.35 +0.1
2,019,029 + Pf6N2E2_1710 0.35 +0.3
2,019,030 - Pf6N2E2_1710 0.35 +1.1
2,019,030 - Pf6N2E2_1710 0.35 -0.5
2,019,030 - Pf6N2E2_1710 0.35 -1.5
2,019,030 - Pf6N2E2_1710 0.35 +0.5
2,019,030 - Pf6N2E2_1710 0.35 +1.5
2,019,030 - Pf6N2E2_1710 0.35 -0.1
2,019,030 - Pf6N2E2_1710 0.35 +1.3
2,019,030 - Pf6N2E2_1710 0.35 +0.3
2,019,031 + Pf6N2E2_1710 0.35 +1.3
2,019,105 + Pf6N2E2_1710 0.41 +0.3
2,019,106 - Pf6N2E2_1710 0.41 +3.4
2,019,123 + Pf6N2E2_1710 0.42 +0.5
2,019,363 + Pf6N2E2_1710 0.61 +0.5
2,019,364 - Pf6N2E2_1710 0.61 -1.1
2,019,364 - Pf6N2E2_1710 0.61 +1.1
2,019,442 - Pf6N2E2_1710 0.67 +0.3
2,019,576 + Pf6N2E2_1710 0.78 +0.4
2,019,577 - Pf6N2E2_1710 0.78 -0.5
2,019,751 - +0.1
2,019,839 - +0.6
2,019,847 + -0.8
2,019,847 + -0.5
2,020,037 + +0.3
2,020,123 + Pf6N2E2_1711 0.17 -0.0
2,020,123 + Pf6N2E2_1711 0.17 -0.3
2,020,124 - Pf6N2E2_1711 0.17 +0.7
2,020,399 + Pf6N2E2_1711 0.48 -0.1
2,020,472 + Pf6N2E2_1711 0.56 +0.5
2,020,473 - Pf6N2E2_1711 0.56 -0.3
2,020,473 - Pf6N2E2_1711 0.56 +0.3

Or see this region's nucleotide sequence