Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1709 and Pf6N2E2_1710 are separated by 43 nucleotides Pf6N2E2_1710 and Pf6N2E2_1711 are separated by 116 nucleotides Pf6N2E2_1711 and Pf6N2E2_1712 overlap by 14 nucleotides
Pf6N2E2_1709 - multidrug resistance transporter, Bcr/CflA family, at 2,017,318 to 2,018,538
_1709
Pf6N2E2_1710 - FIG00953587: hypothetical protein, at 2,018,582 to 2,019,856
_1710
Pf6N2E2_1711 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 2,019,973 to 2,020,857
_1711
Pf6N2E2_1712 - Permease of the drug/metabolite transporter (DMT) superfamily, at 2,020,844 to 2,021,749
_1712
Position (kb)
2018
2019
2020 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2017.904 kb on + strand, within Pf6N2E2_1709 at 2017.904 kb on + strand, within Pf6N2E2_1709 at 2017.976 kb on - strand, within Pf6N2E2_1709 at 2018.120 kb on - strand, within Pf6N2E2_1709 at 2018.120 kb on - strand, within Pf6N2E2_1709 at 2018.281 kb on + strand, within Pf6N2E2_1709 at 2018.281 kb on + strand, within Pf6N2E2_1709 at 2018.675 kb on + strand at 2018.676 kb on - strand at 2018.752 kb on - strand, within Pf6N2E2_1710 at 2019.029 kb on + strand, within Pf6N2E2_1710 at 2019.029 kb on + strand, within Pf6N2E2_1710 at 2019.029 kb on + strand, within Pf6N2E2_1710 at 2019.029 kb on + strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.030 kb on - strand, within Pf6N2E2_1710 at 2019.031 kb on + strand, within Pf6N2E2_1710 at 2019.105 kb on + strand, within Pf6N2E2_1710 at 2019.106 kb on - strand, within Pf6N2E2_1710 at 2019.123 kb on + strand, within Pf6N2E2_1710 at 2019.363 kb on + strand, within Pf6N2E2_1710 at 2019.364 kb on - strand, within Pf6N2E2_1710 at 2019.364 kb on - strand, within Pf6N2E2_1710 at 2019.442 kb on - strand, within Pf6N2E2_1710 at 2019.576 kb on + strand, within Pf6N2E2_1710 at 2019.577 kb on - strand, within Pf6N2E2_1710 at 2019.751 kb on - strand at 2019.839 kb on - strand at 2019.847 kb on + strand at 2019.847 kb on + strand at 2020.037 kb on + strand at 2020.123 kb on + strand, within Pf6N2E2_1711 at 2020.123 kb on + strand, within Pf6N2E2_1711 at 2020.124 kb on - strand, within Pf6N2E2_1711 at 2020.399 kb on + strand, within Pf6N2E2_1711 at 2020.472 kb on + strand, within Pf6N2E2_1711 at 2020.473 kb on - strand, within Pf6N2E2_1711 at 2020.473 kb on - strand, within Pf6N2E2_1711
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 2,017,904 + Pf6N2E2_1709 0.48 -1.1 2,017,904 + Pf6N2E2_1709 0.48 +0.1 2,017,976 - Pf6N2E2_1709 0.54 +0.2 2,018,120 - Pf6N2E2_1709 0.66 -0.1 2,018,120 - Pf6N2E2_1709 0.66 +0.7 2,018,281 + Pf6N2E2_1709 0.79 +0.2 2,018,281 + Pf6N2E2_1709 0.79 +2.7 2,018,675 + -2.1 2,018,676 - +0.0 2,018,752 - Pf6N2E2_1710 0.13 +0.5 2,019,029 + Pf6N2E2_1710 0.35 +1.5 2,019,029 + Pf6N2E2_1710 0.35 +1.5 2,019,029 + Pf6N2E2_1710 0.35 +0.1 2,019,029 + Pf6N2E2_1710 0.35 +0.3 2,019,030 - Pf6N2E2_1710 0.35 +1.1 2,019,030 - Pf6N2E2_1710 0.35 -0.5 2,019,030 - Pf6N2E2_1710 0.35 -1.5 2,019,030 - Pf6N2E2_1710 0.35 +0.5 2,019,030 - Pf6N2E2_1710 0.35 +1.5 2,019,030 - Pf6N2E2_1710 0.35 -0.1 2,019,030 - Pf6N2E2_1710 0.35 +1.3 2,019,030 - Pf6N2E2_1710 0.35 +0.3 2,019,031 + Pf6N2E2_1710 0.35 +1.3 2,019,105 + Pf6N2E2_1710 0.41 +0.3 2,019,106 - Pf6N2E2_1710 0.41 +3.4 2,019,123 + Pf6N2E2_1710 0.42 +0.5 2,019,363 + Pf6N2E2_1710 0.61 +0.5 2,019,364 - Pf6N2E2_1710 0.61 -1.1 2,019,364 - Pf6N2E2_1710 0.61 +1.1 2,019,442 - Pf6N2E2_1710 0.67 +0.3 2,019,576 + Pf6N2E2_1710 0.78 +0.4 2,019,577 - Pf6N2E2_1710 0.78 -0.5 2,019,751 - +0.1 2,019,839 - +0.6 2,019,847 + -0.8 2,019,847 + -0.5 2,020,037 + +0.3 2,020,123 + Pf6N2E2_1711 0.17 -0.0 2,020,123 + Pf6N2E2_1711 0.17 -0.3 2,020,124 - Pf6N2E2_1711 0.17 +0.7 2,020,399 + Pf6N2E2_1711 0.48 -0.1 2,020,472 + Pf6N2E2_1711 0.56 +0.5 2,020,473 - Pf6N2E2_1711 0.56 -0.3 2,020,473 - Pf6N2E2_1711 0.56 +0.3
Or see this region's nucleotide sequence