Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1129 and Pf6N2E2_1130 are separated by 173 nucleotides Pf6N2E2_1130 and Pf6N2E2_1131 are separated by 279 nucleotides Pf6N2E2_1131 and Pf6N2E2_1132 overlap by 1 nucleotides
Pf6N2E2_1129 - Sensor histidine kinase, at 1,325,288 to 1,326,472
_1129
Pf6N2E2_1130 - hypothetical protein, at 1,326,646 to 1,327,167
_1130
Pf6N2E2_1131 - RNA polymerase sigma-70 factor, ECF subfamily, at 1,327,447 to 1,327,866
_1131
Pf6N2E2_1132 - Fe2+-dicitrate sensor, membrane component, at 1,327,866 to 1,328,810
_1132
Position (kb)
1327
1328 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1326.522 kb on + strand at 1326.523 kb on - strand at 1326.548 kb on - strand at 1326.548 kb on - strand at 1326.678 kb on - strand at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.724 kb on + strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.725 kb on - strand, within Pf6N2E2_1130 at 1326.730 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.894 kb on + strand, within Pf6N2E2_1130 at 1326.895 kb on - strand, within Pf6N2E2_1130 at 1326.917 kb on - strand, within Pf6N2E2_1130 at 1327.012 kb on + strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.013 kb on - strand, within Pf6N2E2_1130 at 1327.014 kb on + strand, within Pf6N2E2_1130 at 1327.014 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.078 kb on + strand, within Pf6N2E2_1130 at 1327.079 kb on - strand, within Pf6N2E2_1130 at 1327.238 kb on + strand at 1327.255 kb on + strand at 1327.269 kb on + strand at 1327.269 kb on + strand at 1327.282 kb on + strand at 1327.282 kb on + strand at 1327.283 kb on - strand at 1327.283 kb on - strand at 1327.313 kb on - strand at 1327.402 kb on + strand at 1327.402 kb on + strand at 1327.416 kb on + strand at 1327.546 kb on + strand, within Pf6N2E2_1131 at 1327.546 kb on + strand, within Pf6N2E2_1131 at 1327.546 kb on + strand, within Pf6N2E2_1131 at 1327.546 kb on + strand, within Pf6N2E2_1131 at 1327.612 kb on + strand, within Pf6N2E2_1131 at 1327.822 kb on + strand, within Pf6N2E2_1131 at 1327.822 kb on + strand, within Pf6N2E2_1131 at 1327.823 kb on - strand, within Pf6N2E2_1131 at 1327.823 kb on - strand, within Pf6N2E2_1131 at 1327.823 kb on - strand, within Pf6N2E2_1131 at 1327.837 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,326,522 + -0.7 1,326,523 - -0.1 1,326,548 - -0.1 1,326,548 - +0.6 1,326,678 - +1.0 1,326,724 + Pf6N2E2_1130 0.15 +0.3 1,326,724 + Pf6N2E2_1130 0.15 -0.7 1,326,724 + Pf6N2E2_1130 0.15 -1.2 1,326,725 - Pf6N2E2_1130 0.15 -0.7 1,326,725 - Pf6N2E2_1130 0.15 +0.3 1,326,725 - Pf6N2E2_1130 0.15 -0.7 1,326,725 - Pf6N2E2_1130 0.15 -0.1 1,326,730 + Pf6N2E2_1130 0.16 -0.7 1,326,894 + Pf6N2E2_1130 0.48 -0.7 1,326,894 + Pf6N2E2_1130 0.48 +0.9 1,326,894 + Pf6N2E2_1130 0.48 +0.5 1,326,894 + Pf6N2E2_1130 0.48 +0.9 1,326,895 - Pf6N2E2_1130 0.48 -0.1 1,326,917 - Pf6N2E2_1130 0.52 -0.7 1,327,012 + Pf6N2E2_1130 0.70 +0.3 1,327,013 - Pf6N2E2_1130 0.70 +1.9 1,327,013 - Pf6N2E2_1130 0.70 -0.7 1,327,013 - Pf6N2E2_1130 0.70 -1.3 1,327,014 + Pf6N2E2_1130 0.70 -0.7 1,327,014 + Pf6N2E2_1130 0.70 +1.3 1,327,078 + Pf6N2E2_1130 0.83 -0.4 1,327,078 + Pf6N2E2_1130 0.83 +1.3 1,327,078 + Pf6N2E2_1130 0.83 +0.2 1,327,079 - Pf6N2E2_1130 0.83 +0.4 1,327,238 + -0.7 1,327,255 + -0.7 1,327,269 + +1.1 1,327,269 + +0.9 1,327,282 + +0.8 1,327,282 + -0.7 1,327,283 - -0.9 1,327,283 - -1.7 1,327,313 - +0.3 1,327,402 + -0.7 1,327,402 + -0.7 1,327,416 + +0.3 1,327,546 + Pf6N2E2_1131 0.24 +1.3 1,327,546 + Pf6N2E2_1131 0.24 -1.8 1,327,546 + Pf6N2E2_1131 0.24 -0.7 1,327,546 + Pf6N2E2_1131 0.24 -0.2 1,327,612 + Pf6N2E2_1131 0.39 -0.3 1,327,822 + Pf6N2E2_1131 0.89 -0.7 1,327,822 + Pf6N2E2_1131 0.89 -0.6 1,327,823 - Pf6N2E2_1131 0.90 -0.7 1,327,823 - Pf6N2E2_1131 0.90 +0.3 1,327,823 - Pf6N2E2_1131 0.90 -0.7 1,327,837 + +0.3
Or see this region's nucleotide sequence