Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1113

Experiment: 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1111 and Pf6N2E2_1112 are separated by 2 nucleotidesPf6N2E2_1112 and Pf6N2E2_1113 are separated by 154 nucleotidesPf6N2E2_1113 and Pf6N2E2_1114 are separated by 567 nucleotides Pf6N2E2_1111 - cell wall degradation protein, at 1,303,041 to 1,304,567 _1111 Pf6N2E2_1112 - NAD(P)H oxidoreductase YRKL (EC 1.6.99.-) @ Putative NADPH-quinone reductase (modulator of drug activity B) @ Flavodoxin 2, at 1,304,570 to 1,305,199 _1112 Pf6N2E2_1113 - Transcriptional regulator, TetR family, at 1,305,354 to 1,305,905 _1113 Pf6N2E2_1114 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 1,306,473 to 1,309,034 _1114 Position (kb) 1305 1306Strain fitness (log2 ratio) -1 0 1at 1304.505 kb on + strandat 1304.505 kb on + strandat 1304.506 kb on - strandat 1304.506 kb on - strandat 1304.506 kb on - strandat 1304.520 kb on + strandat 1304.520 kb on + strandat 1304.521 kb on - strandat 1304.766 kb on + strand, within Pf6N2E2_1112at 1305.238 kb on + strandat 1305.239 kb on - strandat 1305.239 kb on - strandat 1305.239 kb on - strandat 1305.249 kb on + strandat 1305.453 kb on + strand, within Pf6N2E2_1113at 1305.496 kb on - strand, within Pf6N2E2_1113at 1305.496 kb on - strand, within Pf6N2E2_1113at 1305.594 kb on + strand, within Pf6N2E2_1113at 1305.688 kb on - strand, within Pf6N2E2_1113at 1306.010 kb on + strandat 1306.010 kb on + strandat 1306.011 kb on - strandat 1306.080 kb on + strandat 1306.349 kb on + strandat 1306.349 kb on + strandat 1306.349 kb on + strandat 1306.350 kb on - strandat 1306.626 kb on + strandat 1306.626 kb on + strandat 1306.627 kb on - strandat 1306.651 kb on - strandat 1306.701 kb on + strandat 1306.702 kb on - strandat 1306.798 kb on - strand, within Pf6N2E2_1114at 1306.878 kb on + strand, within Pf6N2E2_1114at 1306.879 kb on - strand, within Pf6N2E2_1114at 1306.879 kb on - strand, within Pf6N2E2_1114

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Per-strain Table

Position Strand Gene LocusTag Fraction 10% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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1,304,505 + -0.1
1,304,505 + -0.0
1,304,506 - +0.3
1,304,506 - +0.3
1,304,506 - +1.0
1,304,520 + -0.5
1,304,520 + -1.5
1,304,521 - -0.7
1,304,766 + Pf6N2E2_1112 0.31 +0.3
1,305,238 + +1.5
1,305,239 - -0.3
1,305,239 - +1.3
1,305,239 - -1.3
1,305,249 + -1.7
1,305,453 + Pf6N2E2_1113 0.18 +0.9
1,305,496 - Pf6N2E2_1113 0.26 -1.3
1,305,496 - Pf6N2E2_1113 0.26 -0.7
1,305,594 + Pf6N2E2_1113 0.43 -0.1
1,305,688 - Pf6N2E2_1113 0.61 -0.7
1,306,010 + +0.5
1,306,010 + +0.9
1,306,011 - -1.2
1,306,080 + +0.7
1,306,349 + -0.1
1,306,349 + +0.2
1,306,349 + -0.2
1,306,350 - +0.9
1,306,626 + +1.1
1,306,626 + -0.7
1,306,627 - +0.3
1,306,651 - +0.2
1,306,701 + +0.9
1,306,702 - -0.7
1,306,798 - Pf6N2E2_1114 0.13 +0.7
1,306,878 + Pf6N2E2_1114 0.16 +0.9
1,306,879 - Pf6N2E2_1114 0.16 -0.5
1,306,879 - Pf6N2E2_1114 0.16 -0.3

Or see this region's nucleotide sequence