Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_934 and Pf6N2E2_935 are separated by 78 nucleotides Pf6N2E2_935 and Pf6N2E2_936 are separated by 102 nucleotides
Pf6N2E2_934 - Arabinose 5-phosphate isomerase (EC 5.3.1.13), at 1,101,075 to 1,101,671
_934
Pf6N2E2_935 - Aldehyde dehydrogenase (EC 1.2.1.3), at 1,101,750 to 1,103,156
_935
Pf6N2E2_936 - NADH:flavin oxidoreductase/NADH oxidase, at 1,103,259 to 1,104,380
_936
Position (kb)
1101
1102
1103
1104 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1100.830 kb on + strand at 1100.830 kb on + strand at 1100.830 kb on + strand at 1100.830 kb on + strand at 1100.831 kb on - strand at 1100.831 kb on - strand at 1100.831 kb on - strand at 1100.831 kb on - strand at 1100.831 kb on - strand at 1100.831 kb on - strand at 1100.831 kb on - strand at 1100.831 kb on - strand at 1100.880 kb on + strand at 1101.044 kb on - strand at 1101.106 kb on - strand at 1101.235 kb on + strand, within Pf6N2E2_934 at 1101.336 kb on - strand, within Pf6N2E2_934 at 1101.641 kb on + strand at 1101.642 kb on - strand at 1101.700 kb on + strand at 1102.004 kb on + strand, within Pf6N2E2_935 at 1102.004 kb on + strand, within Pf6N2E2_935 at 1102.005 kb on - strand, within Pf6N2E2_935 at 1102.005 kb on - strand, within Pf6N2E2_935 at 1102.008 kb on + strand, within Pf6N2E2_935 at 1102.008 kb on + strand, within Pf6N2E2_935 at 1102.008 kb on + strand, within Pf6N2E2_935 at 1102.008 kb on + strand, within Pf6N2E2_935 at 1102.009 kb on - strand, within Pf6N2E2_935 at 1102.034 kb on - strand, within Pf6N2E2_935 at 1102.034 kb on - strand, within Pf6N2E2_935 at 1102.034 kb on - strand, within Pf6N2E2_935 at 1102.096 kb on - strand, within Pf6N2E2_935 at 1102.114 kb on + strand, within Pf6N2E2_935 at 1102.115 kb on - strand, within Pf6N2E2_935 at 1102.137 kb on - strand, within Pf6N2E2_935 at 1102.244 kb on + strand, within Pf6N2E2_935 at 1102.245 kb on - strand, within Pf6N2E2_935 at 1102.271 kb on + strand, within Pf6N2E2_935 at 1102.271 kb on + strand, within Pf6N2E2_935 at 1102.271 kb on + strand, within Pf6N2E2_935 at 1102.272 kb on - strand, within Pf6N2E2_935 at 1102.272 kb on - strand, within Pf6N2E2_935 at 1102.272 kb on - strand, within Pf6N2E2_935 at 1102.272 kb on - strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.275 kb on + strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.276 kb on - strand, within Pf6N2E2_935 at 1102.278 kb on - strand, within Pf6N2E2_935 at 1102.520 kb on + strand, within Pf6N2E2_935 at 1102.530 kb on + strand, within Pf6N2E2_935 at 1102.530 kb on + strand, within Pf6N2E2_935 at 1102.531 kb on - strand, within Pf6N2E2_935 at 1102.562 kb on + strand, within Pf6N2E2_935 at 1102.671 kb on + strand, within Pf6N2E2_935 at 1102.762 kb on - strand, within Pf6N2E2_935 at 1102.808 kb on + strand, within Pf6N2E2_935 at 1102.809 kb on - strand, within Pf6N2E2_935 at 1102.815 kb on + strand, within Pf6N2E2_935 at 1102.893 kb on + strand, within Pf6N2E2_935 at 1102.893 kb on + strand, within Pf6N2E2_935 at 1102.893 kb on + strand, within Pf6N2E2_935 at 1102.999 kb on + strand, within Pf6N2E2_935 at 1103.001 kb on + strand, within Pf6N2E2_935 at 1103.002 kb on - strand, within Pf6N2E2_935 at 1103.123 kb on - strand at 1103.133 kb on + strand at 1103.133 kb on + strand at 1103.134 kb on - strand at 1103.167 kb on + strand at 1103.168 kb on - strand at 1103.168 kb on - strand at 1103.260 kb on + strand at 1103.261 kb on - strand at 1103.278 kb on + strand at 1103.287 kb on + strand at 1103.287 kb on + strand at 1103.287 kb on + strand at 1103.288 kb on - strand at 1103.288 kb on - strand at 1103.308 kb on + strand at 1103.309 kb on - strand at 1103.309 kb on - strand at 1103.311 kb on + strand at 1103.312 kb on - strand at 1103.312 kb on - strand at 1103.382 kb on - strand, within Pf6N2E2_936 at 1103.382 kb on - strand, within Pf6N2E2_936 at 1103.595 kb on - strand, within Pf6N2E2_936 at 1103.629 kb on + strand, within Pf6N2E2_936 at 1103.629 kb on + strand, within Pf6N2E2_936 at 1103.650 kb on + strand, within Pf6N2E2_936 at 1103.687 kb on + strand, within Pf6N2E2_936 at 1103.877 kb on + strand, within Pf6N2E2_936 at 1103.989 kb on + strand, within Pf6N2E2_936 at 1103.990 kb on - strand, within Pf6N2E2_936 at 1103.995 kb on + strand, within Pf6N2E2_936 at 1103.995 kb on + strand, within Pf6N2E2_936
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,100,830 + -0.5 1,100,830 + +0.8 1,100,830 + +1.5 1,100,830 + -0.5 1,100,831 - -0.5 1,100,831 - +0.5 1,100,831 - +1.6 1,100,831 - +0.5 1,100,831 - +1.8 1,100,831 - +1.5 1,100,831 - +1.0 1,100,831 - +0.8 1,100,880 + -1.1 1,101,044 - +0.4 1,101,106 - -0.3 1,101,235 + Pf6N2E2_934 0.27 -1.3 1,101,336 - Pf6N2E2_934 0.44 -0.1 1,101,641 + -0.6 1,101,642 - -0.6 1,101,700 + +0.6 1,102,004 + Pf6N2E2_935 0.18 +0.9 1,102,004 + Pf6N2E2_935 0.18 +1.3 1,102,005 - Pf6N2E2_935 0.18 +0.4 1,102,005 - Pf6N2E2_935 0.18 -0.4 1,102,008 + Pf6N2E2_935 0.18 +0.4 1,102,008 + Pf6N2E2_935 0.18 -0.0 1,102,008 + Pf6N2E2_935 0.18 -0.2 1,102,008 + Pf6N2E2_935 0.18 -0.8 1,102,009 - Pf6N2E2_935 0.18 +0.4 1,102,034 - Pf6N2E2_935 0.20 -1.1 1,102,034 - Pf6N2E2_935 0.20 -0.1 1,102,034 - Pf6N2E2_935 0.20 +0.8 1,102,096 - Pf6N2E2_935 0.25 +1.4 1,102,114 + Pf6N2E2_935 0.26 +1.3 1,102,115 - Pf6N2E2_935 0.26 +0.4 1,102,137 - Pf6N2E2_935 0.28 +1.0 1,102,244 + Pf6N2E2_935 0.35 -1.1 1,102,245 - Pf6N2E2_935 0.35 +0.5 1,102,271 + Pf6N2E2_935 0.37 +0.9 1,102,271 + Pf6N2E2_935 0.37 +0.4 1,102,271 + Pf6N2E2_935 0.37 -0.6 1,102,272 - Pf6N2E2_935 0.37 +0.3 1,102,272 - Pf6N2E2_935 0.37 +0.5 1,102,272 - Pf6N2E2_935 0.37 +1.0 1,102,272 - Pf6N2E2_935 0.37 -0.3 1,102,275 + Pf6N2E2_935 0.37 -0.6 1,102,275 + Pf6N2E2_935 0.37 +1.3 1,102,275 + Pf6N2E2_935 0.37 -0.3 1,102,275 + Pf6N2E2_935 0.37 -0.2 1,102,275 + Pf6N2E2_935 0.37 -1.1 1,102,275 + Pf6N2E2_935 0.37 +1.0 1,102,275 + Pf6N2E2_935 0.37 +0.4 1,102,275 + Pf6N2E2_935 0.37 -1.6 1,102,276 - Pf6N2E2_935 0.37 +0.9 1,102,276 - Pf6N2E2_935 0.37 +0.2 1,102,276 - Pf6N2E2_935 0.37 +0.4 1,102,276 - Pf6N2E2_935 0.37 +1.0 1,102,276 - Pf6N2E2_935 0.37 -0.6 1,102,276 - Pf6N2E2_935 0.37 -0.1 1,102,276 - Pf6N2E2_935 0.37 +0.4 1,102,276 - Pf6N2E2_935 0.37 +0.0 1,102,276 - Pf6N2E2_935 0.37 +0.7 1,102,278 - Pf6N2E2_935 0.38 -0.6 1,102,520 + Pf6N2E2_935 0.55 +0.4 1,102,530 + Pf6N2E2_935 0.55 +0.4 1,102,530 + Pf6N2E2_935 0.55 +0.2 1,102,531 - Pf6N2E2_935 0.56 +1.5 1,102,562 + Pf6N2E2_935 0.58 -2.1 1,102,671 + Pf6N2E2_935 0.65 +0.8 1,102,762 - Pf6N2E2_935 0.72 +0.3 1,102,808 + Pf6N2E2_935 0.75 +0.6 1,102,809 - Pf6N2E2_935 0.75 +1.4 1,102,815 + Pf6N2E2_935 0.76 +0.4 1,102,893 + Pf6N2E2_935 0.81 +0.4 1,102,893 + Pf6N2E2_935 0.81 +0.4 1,102,893 + Pf6N2E2_935 0.81 -0.4 1,102,999 + Pf6N2E2_935 0.89 -2.1 1,103,001 + Pf6N2E2_935 0.89 -0.1 1,103,002 - Pf6N2E2_935 0.89 +0.1 1,103,123 - -0.6 1,103,133 + +0.0 1,103,133 + +0.0 1,103,134 - -0.6 1,103,167 + +0.7 1,103,168 - -0.2 1,103,168 - +0.4 1,103,260 + +0.3 1,103,261 - -0.8 1,103,278 + +0.0 1,103,287 + -0.9 1,103,287 + +0.8 1,103,287 + +2.0 1,103,288 - -1.6 1,103,288 - -2.1 1,103,308 + -0.3 1,103,309 - -0.6 1,103,309 - -2.1 1,103,311 + -0.6 1,103,312 - -0.1 1,103,312 - -2.4 1,103,382 - Pf6N2E2_936 0.11 -0.0 1,103,382 - Pf6N2E2_936 0.11 -1.6 1,103,595 - Pf6N2E2_936 0.30 -0.1 1,103,629 + Pf6N2E2_936 0.33 +0.4 1,103,629 + Pf6N2E2_936 0.33 -1.0 1,103,650 + Pf6N2E2_936 0.35 -0.6 1,103,687 + Pf6N2E2_936 0.38 -1.6 1,103,877 + Pf6N2E2_936 0.55 -0.0 1,103,989 + Pf6N2E2_936 0.65 -0.8 1,103,990 - Pf6N2E2_936 0.65 -1.7 1,103,995 + Pf6N2E2_936 0.66 -0.6 1,103,995 + Pf6N2E2_936 0.66 +1.0
Or see this region's nucleotide sequence