Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_871 and Pf6N2E2_872 overlap by 17 nucleotides Pf6N2E2_872 and Pf6N2E2_873 are separated by 199 nucleotides
Pf6N2E2_871 - sensor histidine kinase, at 1,019,530 to 1,020,912
_871
Pf6N2E2_872 - TonB-dependent receptor; Outer membrane receptor for ferrienterochelin and colicins, at 1,020,896 to 1,022,863
_872
Pf6N2E2_873 - Methyl-accepting chemotaxis protein, at 1,023,063 to 1,024,352
_873
Position (kb)
1020
1021
1022
1023 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1020.031 kb on + strand, within Pf6N2E2_871 at 1020.032 kb on - strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.055 kb on + strand, within Pf6N2E2_871 at 1020.056 kb on - strand, within Pf6N2E2_871 at 1020.056 kb on - strand, within Pf6N2E2_871 at 1020.056 kb on - strand, within Pf6N2E2_871 at 1020.056 kb on - strand, within Pf6N2E2_871 at 1020.056 kb on - strand at 1020.309 kb on + strand, within Pf6N2E2_871 at 1020.479 kb on + strand, within Pf6N2E2_871 at 1020.897 kb on + strand at 1020.897 kb on + strand at 1020.898 kb on - strand at 1020.936 kb on + strand at 1020.936 kb on + strand at 1020.937 kb on - strand at 1020.937 kb on - strand at 1020.937 kb on - strand at 1020.951 kb on + strand at 1020.952 kb on - strand at 1021.026 kb on + strand at 1021.026 kb on + strand at 1021.027 kb on - strand at 1021.027 kb on - strand at 1021.102 kb on - strand, within Pf6N2E2_872 at 1021.104 kb on + strand, within Pf6N2E2_872 at 1021.104 kb on + strand, within Pf6N2E2_872 at 1021.104 kb on + strand, within Pf6N2E2_872 at 1021.104 kb on + strand, within Pf6N2E2_872 at 1021.104 kb on + strand, within Pf6N2E2_872 at 1021.105 kb on - strand, within Pf6N2E2_872 at 1021.233 kb on + strand, within Pf6N2E2_872 at 1021.233 kb on + strand, within Pf6N2E2_872 at 1021.233 kb on + strand, within Pf6N2E2_872 at 1021.234 kb on - strand, within Pf6N2E2_872 at 1021.239 kb on + strand, within Pf6N2E2_872 at 1021.239 kb on + strand, within Pf6N2E2_872 at 1021.239 kb on + strand, within Pf6N2E2_872 at 1021.239 kb on + strand, within Pf6N2E2_872 at 1021.240 kb on - strand, within Pf6N2E2_872 at 1021.240 kb on - strand, within Pf6N2E2_872 at 1021.341 kb on + strand, within Pf6N2E2_872 at 1021.342 kb on - strand, within Pf6N2E2_872 at 1021.398 kb on + strand, within Pf6N2E2_872 at 1021.398 kb on + strand, within Pf6N2E2_872 at 1021.398 kb on + strand, within Pf6N2E2_872 at 1021.399 kb on - strand, within Pf6N2E2_872 at 1021.458 kb on + strand, within Pf6N2E2_872 at 1021.530 kb on + strand, within Pf6N2E2_872 at 1021.650 kb on + strand, within Pf6N2E2_872 at 1021.650 kb on + strand, within Pf6N2E2_872 at 1021.756 kb on - strand, within Pf6N2E2_872 at 1021.899 kb on + strand, within Pf6N2E2_872 at 1021.899 kb on + strand, within Pf6N2E2_872 at 1021.899 kb on + strand, within Pf6N2E2_872 at 1021.903 kb on + strand, within Pf6N2E2_872 at 1022.212 kb on - strand, within Pf6N2E2_872 at 1022.266 kb on - strand, within Pf6N2E2_872 at 1022.317 kb on - strand, within Pf6N2E2_872 at 1022.544 kb on + strand, within Pf6N2E2_872 at 1022.544 kb on + strand, within Pf6N2E2_872 at 1022.875 kb on + strand at 1022.875 kb on + strand at 1022.875 kb on + strand at 1022.876 kb on - strand at 1022.876 kb on - strand at 1022.876 kb on - strand at 1022.970 kb on + strand at 1022.971 kb on - strand at 1023.040 kb on + strand at 1023.040 kb on + strand at 1023.040 kb on + strand at 1023.040 kb on + strand at 1023.041 kb on - strand at 1023.041 kb on - strand at 1023.041 kb on - strand at 1023.067 kb on + strand at 1023.067 kb on + strand at 1023.068 kb on - strand at 1023.186 kb on + strand at 1023.186 kb on + strand at 1023.187 kb on - strand at 1023.187 kb on - strand at 1023.187 kb on - strand at 1023.187 kb on - strand at 1023.207 kb on + strand, within Pf6N2E2_873 at 1023.359 kb on + strand, within Pf6N2E2_873 at 1023.359 kb on + strand, within Pf6N2E2_873 at 1023.359 kb on + strand, within Pf6N2E2_873 at 1023.359 kb on + strand, within Pf6N2E2_873 at 1023.360 kb on - strand, within Pf6N2E2_873 at 1023.360 kb on - strand, within Pf6N2E2_873 at 1023.418 kb on + strand, within Pf6N2E2_873 at 1023.419 kb on - strand, within Pf6N2E2_873 at 1023.656 kb on + strand, within Pf6N2E2_873 at 1023.656 kb on + strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.657 kb on - strand, within Pf6N2E2_873 at 1023.835 kb on + strand, within Pf6N2E2_873 at 1023.835 kb on + strand, within Pf6N2E2_873
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 1,020,031 + Pf6N2E2_871 0.36 -0.1 1,020,032 - Pf6N2E2_871 0.36 -0.9 1,020,055 + Pf6N2E2_871 0.38 -0.2 1,020,055 + Pf6N2E2_871 0.38 -0.5 1,020,055 + Pf6N2E2_871 0.38 +0.5 1,020,055 + Pf6N2E2_871 0.38 -1.7 1,020,055 + Pf6N2E2_871 0.38 +0.9 1,020,055 + Pf6N2E2_871 0.38 -0.2 1,020,056 - Pf6N2E2_871 0.38 -0.1 1,020,056 - Pf6N2E2_871 0.38 +1.3 1,020,056 - Pf6N2E2_871 0.38 +0.4 1,020,056 - Pf6N2E2_871 0.38 +0.1 1,020,056 - +0.5 1,020,309 + Pf6N2E2_871 0.56 +0.3 1,020,479 + Pf6N2E2_871 0.69 +1.0 1,020,897 + -1.1 1,020,897 + +0.2 1,020,898 - +0.0 1,020,936 + +0.1 1,020,936 + +1.0 1,020,937 - +1.0 1,020,937 - +1.2 1,020,937 - +0.3 1,020,951 + +1.1 1,020,952 - +0.1 1,021,026 + +0.3 1,021,026 + +0.8 1,021,027 - +0.2 1,021,027 - -0.5 1,021,102 - Pf6N2E2_872 0.10 -0.5 1,021,104 + Pf6N2E2_872 0.11 -1.5 1,021,104 + Pf6N2E2_872 0.11 +1.5 1,021,104 + Pf6N2E2_872 0.11 +0.6 1,021,104 + Pf6N2E2_872 0.11 +1.1 1,021,104 + Pf6N2E2_872 0.11 +0.3 1,021,105 - Pf6N2E2_872 0.11 +1.1 1,021,233 + Pf6N2E2_872 0.17 +0.9 1,021,233 + Pf6N2E2_872 0.17 +1.0 1,021,233 + Pf6N2E2_872 0.17 -0.5 1,021,234 - Pf6N2E2_872 0.17 +1.1 1,021,239 + Pf6N2E2_872 0.17 +0.5 1,021,239 + Pf6N2E2_872 0.17 +0.8 1,021,239 + Pf6N2E2_872 0.17 +0.3 1,021,239 + Pf6N2E2_872 0.17 +1.1 1,021,240 - Pf6N2E2_872 0.17 -0.5 1,021,240 - Pf6N2E2_872 0.17 +0.7 1,021,341 + Pf6N2E2_872 0.23 +0.7 1,021,342 - Pf6N2E2_872 0.23 -0.5 1,021,398 + Pf6N2E2_872 0.26 +0.5 1,021,398 + Pf6N2E2_872 0.26 +0.2 1,021,398 + Pf6N2E2_872 0.26 +1.1 1,021,399 - Pf6N2E2_872 0.26 +0.6 1,021,458 + Pf6N2E2_872 0.29 -0.1 1,021,530 + Pf6N2E2_872 0.32 +0.1 1,021,650 + Pf6N2E2_872 0.38 +1.0 1,021,650 + Pf6N2E2_872 0.38 -0.5 1,021,756 - Pf6N2E2_872 0.44 +0.5 1,021,899 + Pf6N2E2_872 0.51 -0.5 1,021,899 + Pf6N2E2_872 0.51 +0.3 1,021,899 + Pf6N2E2_872 0.51 +0.8 1,021,903 + Pf6N2E2_872 0.51 +0.2 1,022,212 - Pf6N2E2_872 0.67 -0.3 1,022,266 - Pf6N2E2_872 0.70 -1.5 1,022,317 - Pf6N2E2_872 0.72 -2.5 1,022,544 + Pf6N2E2_872 0.84 -1.2 1,022,544 + Pf6N2E2_872 0.84 -1.2 1,022,875 + -0.1 1,022,875 + +0.3 1,022,875 + +1.1 1,022,876 - +0.6 1,022,876 - -0.6 1,022,876 - -1.3 1,022,970 + -0.9 1,022,971 - -1.5 1,023,040 + +0.1 1,023,040 + +1.1 1,023,040 + +0.4 1,023,040 + +0.6 1,023,041 - +1.1 1,023,041 - -0.1 1,023,041 - -0.0 1,023,067 + -0.3 1,023,067 + +0.7 1,023,068 - +0.8 1,023,186 + +1.4 1,023,186 + +0.2 1,023,187 - -2.8 1,023,187 - -0.2 1,023,187 - +0.5 1,023,187 - +1.4 1,023,207 + Pf6N2E2_873 0.11 +0.5 1,023,359 + Pf6N2E2_873 0.23 -0.5 1,023,359 + Pf6N2E2_873 0.23 -0.4 1,023,359 + Pf6N2E2_873 0.23 -0.3 1,023,359 + Pf6N2E2_873 0.23 +2.0 1,023,360 - Pf6N2E2_873 0.23 -0.5 1,023,360 - Pf6N2E2_873 0.23 +0.3 1,023,418 + Pf6N2E2_873 0.28 +0.5 1,023,419 - Pf6N2E2_873 0.28 +0.7 1,023,656 + Pf6N2E2_873 0.46 -0.1 1,023,656 + Pf6N2E2_873 0.46 +1.3 1,023,657 - Pf6N2E2_873 0.46 +0.3 1,023,657 - Pf6N2E2_873 0.46 +0.3 1,023,657 - Pf6N2E2_873 0.46 -2.1 1,023,657 - Pf6N2E2_873 0.46 -0.9 1,023,835 + Pf6N2E2_873 0.60 +0.5 1,023,835 + Pf6N2E2_873 0.60 -0.5
Or see this region's nucleotide sequence