Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_664

Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_663 and Pf6N2E2_664 are separated by 342 nucleotidesPf6N2E2_664 and Pf6N2E2_665 are separated by 2 nucleotides Pf6N2E2_663 - Transcriptional activator of acetoin dehydrogenase operon AcoR, at 766,744 to 768,609 _663 Pf6N2E2_664 - Acetoin catabolism protein X, at 768,952 to 770,025 _664 Pf6N2E2_665 - Acetoin dehydrogenase E1 component alpha-subunit (EC 1.2.4.-), at 770,028 to 771,005 _665 Position (kb) 768 769 770 771Strain fitness (log2 ratio) -1 0 1 2at 768.248 kb on + strand, within Pf6N2E2_663at 768.609 kb on + strandat 768.610 kb on - strandat 768.649 kb on + strandat 768.887 kb on - strandat 768.887 kb on - strandat 768.890 kb on - strandat 768.904 kb on + strandat 768.905 kb on - strandat 768.905 kb on - strandat 769.432 kb on + strand, within Pf6N2E2_664at 769.433 kb on - strand, within Pf6N2E2_664at 769.433 kb on - strand, within Pf6N2E2_664at 769.939 kb on + strandat 770.155 kb on - strand, within Pf6N2E2_665at 770.155 kb on - strand, within Pf6N2E2_665at 770.289 kb on + strand, within Pf6N2E2_665at 770.289 kb on + strand, within Pf6N2E2_665at 770.290 kb on - strand, within Pf6N2E2_665at 770.560 kb on - strand, within Pf6N2E2_665at 770.934 kb on + strandat 770.988 kb on + strandat 770.989 kb on - strandat 770.998 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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768,248 + Pf6N2E2_663 0.81 +0.9
768,609 + +0.5
768,610 - +1.9
768,649 + -0.4
768,887 - +0.2
768,887 - +0.4
768,890 - +1.2
768,904 + +0.9
768,905 - -0.4
768,905 - -0.4
769,432 + Pf6N2E2_664 0.45 -0.4
769,433 - Pf6N2E2_664 0.45 -1.4
769,433 - Pf6N2E2_664 0.45 -0.4
769,939 + +0.9
770,155 - Pf6N2E2_665 0.13 -0.4
770,155 - Pf6N2E2_665 0.13 +0.0
770,289 + Pf6N2E2_665 0.27 +0.6
770,289 + Pf6N2E2_665 0.27 +0.3
770,290 - Pf6N2E2_665 0.27 +0.7
770,560 - Pf6N2E2_665 0.54 +0.3
770,934 + -0.4
770,988 + +0.1
770,989 - +0.7
770,998 - +0.3

Or see this region's nucleotide sequence