Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_538 and Pf6N2E2_539 are separated by 6 nucleotides Pf6N2E2_539 and Pf6N2E2_540 overlap by 4 nucleotides Pf6N2E2_540 and Pf6N2E2_541 are separated by 147 nucleotides
Pf6N2E2_538 - Na(+) H(+) antiporter subunit A; Na(+) H(+) antiporter subunit B, at 604,870 to 607,794
_538
Pf6N2E2_539 - hypothetical protein, at 607,801 to 608,004
_539
Pf6N2E2_540 - Permease of the drug/metabolite transporter (DMT) superfamily, at 608,001 to 608,870
_540
Pf6N2E2_541 - Transcriptional regulator, Cro/CI family, at 609,018 to 609,581
_541
Position (kb) 608
609 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 607.067 kb on + strand, within Pf6N2E2_538 at 607.067 kb on + strand, within Pf6N2E2_538 at 607.195 kb on + strand, within Pf6N2E2_538 at 607.195 kb on + strand, within Pf6N2E2_538 at 607.195 kb on + strand, within Pf6N2E2_538 at 607.195 kb on + strand, within Pf6N2E2_538 at 607.195 kb on + strand, within Pf6N2E2_538 at 607.195 kb on + strand, within Pf6N2E2_538 at 607.196 kb on - strand, within Pf6N2E2_538 at 607.233 kb on - strand, within Pf6N2E2_538 at 607.233 kb on - strand, within Pf6N2E2_538 at 607.233 kb on - strand, within Pf6N2E2_538 at 607.233 kb on - strand, within Pf6N2E2_538 at 607.233 kb on - strand, within Pf6N2E2_538 at 607.311 kb on + strand, within Pf6N2E2_538 at 607.312 kb on - strand, within Pf6N2E2_538 at 607.312 kb on - strand, within Pf6N2E2_538 at 607.312 kb on - strand, within Pf6N2E2_538 at 607.312 kb on - strand, within Pf6N2E2_538 at 607.312 kb on - strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.313 kb on + strand, within Pf6N2E2_538 at 607.314 kb on - strand, within Pf6N2E2_538 at 607.314 kb on - strand, within Pf6N2E2_538 at 607.314 kb on - strand, within Pf6N2E2_538 at 607.314 kb on - strand, within Pf6N2E2_538 at 607.314 kb on - strand, within Pf6N2E2_538 at 607.314 kb on - strand, within Pf6N2E2_538 at 607.314 kb on - strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.499 kb on + strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.500 kb on - strand, within Pf6N2E2_538 at 607.511 kb on + strand at 607.512 kb on - strand at 607.616 kb on + strand at 607.616 kb on + strand at 607.617 kb on - strand at 607.617 kb on - strand at 607.617 kb on - strand at 607.617 kb on - strand at 607.617 kb on - strand at 607.617 kb on - strand at 607.777 kb on + strand at 607.777 kb on + strand at 607.777 kb on + strand at 607.783 kb on + strand at 607.784 kb on - strand at 607.784 kb on - strand at 607.784 kb on - strand at 607.794 kb on + strand at 607.794 kb on + strand at 607.795 kb on - strand at 607.795 kb on - strand at 607.795 kb on - strand at 607.802 kb on + strand at 607.803 kb on - strand at 607.829 kb on + strand, within Pf6N2E2_539 at 607.830 kb on - strand, within Pf6N2E2_539 at 607.830 kb on - strand, within Pf6N2E2_539 at 607.876 kb on + strand, within Pf6N2E2_539 at 607.877 kb on - strand, within Pf6N2E2_539 at 607.883 kb on + strand, within Pf6N2E2_539 at 607.883 kb on + strand, within Pf6N2E2_539 at 607.883 kb on + strand, within Pf6N2E2_539 at 607.883 kb on + strand, within Pf6N2E2_539 at 607.883 kb on + strand, within Pf6N2E2_539 at 607.884 kb on - strand, within Pf6N2E2_539 at 607.884 kb on - strand, within Pf6N2E2_539 at 607.884 kb on - strand, within Pf6N2E2_539 at 607.884 kb on - strand, within Pf6N2E2_539 at 607.884 kb on - strand, within Pf6N2E2_539 at 607.884 kb on - strand, within Pf6N2E2_539 at 607.884 kb on - strand, within Pf6N2E2_539 at 608.173 kb on + strand, within Pf6N2E2_540 at 608.173 kb on + strand, within Pf6N2E2_540 at 608.173 kb on + strand, within Pf6N2E2_540 at 608.173 kb on + strand, within Pf6N2E2_540 at 608.173 kb on + strand, within Pf6N2E2_540 at 608.173 kb on + strand, within Pf6N2E2_540 at 608.173 kb on + strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.174 kb on - strand, within Pf6N2E2_540 at 608.596 kb on + strand, within Pf6N2E2_540 at 608.596 kb on + strand, within Pf6N2E2_540 at 608.597 kb on - strand, within Pf6N2E2_540 at 608.597 kb on - strand, within Pf6N2E2_540 at 608.985 kb on + strand at 608.985 kb on + strand at 608.986 kb on - strand at 608.986 kb on - strand at 609.015 kb on - strand at 609.017 kb on - strand at 609.220 kb on + strand, within Pf6N2E2_541 at 609.387 kb on + strand, within Pf6N2E2_541 at 609.388 kb on - strand, within Pf6N2E2_541 at 609.429 kb on + strand, within Pf6N2E2_541 at 609.429 kb on + strand, within Pf6N2E2_541 at 609.429 kb on + strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.430 kb on - strand, within Pf6N2E2_541 at 609.562 kb on + strand at 609.629 kb on + strand at 609.629 kb on + strand at 609.630 kb on - strand at 609.650 kb on + strand at 609.650 kb on + strand at 609.651 kb on - strand at 609.651 kb on - strand at 609.686 kb on + strand at 609.687 kb on - strand at 609.710 kb on - strand at 609.747 kb on + strand at 609.747 kb on + strand at 609.748 kb on - strand at 609.752 kb on + strand at 609.753 kb on - strand at 609.816 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 607,067 + Pf6N2E2_538 0.75 +1.9 607,067 + Pf6N2E2_538 0.75 +0.4 607,195 + Pf6N2E2_538 0.79 -0.7 607,195 + Pf6N2E2_538 0.79 -1.4 607,195 + Pf6N2E2_538 0.79 -0.4 607,195 + Pf6N2E2_538 0.79 +0.6 607,195 + Pf6N2E2_538 0.79 +0.6 607,195 + Pf6N2E2_538 0.79 +0.6 607,196 - Pf6N2E2_538 0.80 -1.4 607,233 - Pf6N2E2_538 0.81 -2.3 607,233 - Pf6N2E2_538 0.81 +1.9 607,233 - Pf6N2E2_538 0.81 -0.4 607,233 - Pf6N2E2_538 0.81 -0.4 607,233 - Pf6N2E2_538 0.81 -2.0 607,311 + Pf6N2E2_538 0.83 +0.0 607,312 - Pf6N2E2_538 0.83 -0.4 607,312 - Pf6N2E2_538 0.83 -0.2 607,312 - Pf6N2E2_538 0.83 -1.2 607,312 - Pf6N2E2_538 0.83 +0.4 607,312 - Pf6N2E2_538 0.83 +0.2 607,313 + Pf6N2E2_538 0.84 -0.4 607,313 + Pf6N2E2_538 0.84 -0.4 607,313 + Pf6N2E2_538 0.84 -0.3 607,313 + Pf6N2E2_538 0.84 +0.1 607,313 + Pf6N2E2_538 0.84 +0.6 607,313 + Pf6N2E2_538 0.84 +0.8 607,313 + Pf6N2E2_538 0.84 -0.4 607,313 + Pf6N2E2_538 0.84 -0.4 607,313 + Pf6N2E2_538 0.84 +1.6 607,314 - Pf6N2E2_538 0.84 -0.4 607,314 - Pf6N2E2_538 0.84 +0.6 607,314 - Pf6N2E2_538 0.84 -0.1 607,314 - Pf6N2E2_538 0.84 -0.0 607,314 - Pf6N2E2_538 0.84 -0.1 607,314 - Pf6N2E2_538 0.84 -0.2 607,314 - Pf6N2E2_538 0.84 +0.9 607,499 + Pf6N2E2_538 0.90 +0.6 607,499 + Pf6N2E2_538 0.90 +0.6 607,499 + Pf6N2E2_538 0.90 +0.6 607,499 + Pf6N2E2_538 0.90 -1.1 607,499 + Pf6N2E2_538 0.90 -0.4 607,499 + Pf6N2E2_538 0.90 +1.4 607,499 + Pf6N2E2_538 0.90 +1.3 607,499 + Pf6N2E2_538 0.90 -0.4 607,499 + Pf6N2E2_538 0.90 +0.6 607,500 - Pf6N2E2_538 0.90 -0.4 607,500 - Pf6N2E2_538 0.90 -0.1 607,500 - Pf6N2E2_538 0.90 +0.0 607,500 - Pf6N2E2_538 0.90 +0.6 607,500 - Pf6N2E2_538 0.90 +0.6 607,500 - Pf6N2E2_538 0.90 -0.1 607,500 - Pf6N2E2_538 0.90 -0.0 607,500 - Pf6N2E2_538 0.90 -1.0 607,500 - Pf6N2E2_538 0.90 +1.2 607,500 - Pf6N2E2_538 0.90 +0.6 607,500 - Pf6N2E2_538 0.90 -0.4 607,511 + -0.4 607,512 - +0.6 607,616 + -0.4 607,616 + -0.4 607,617 - -0.4 607,617 - -0.4 607,617 - +0.0 607,617 - -0.4 607,617 - +0.0 607,617 - -0.4 607,777 + +0.4 607,777 + +0.4 607,777 + +0.3 607,783 + +0.9 607,784 - +0.6 607,784 - -0.6 607,784 - +0.4 607,794 + -0.1 607,794 + -0.4 607,795 - -0.5 607,795 - +0.2 607,795 - -0.9 607,802 + -0.5 607,803 - -0.4 607,829 + Pf6N2E2_539 0.14 -0.6 607,830 - Pf6N2E2_539 0.14 -0.4 607,830 - Pf6N2E2_539 0.14 +0.8 607,876 + Pf6N2E2_539 0.37 +0.2 607,877 - Pf6N2E2_539 0.37 +0.4 607,883 + Pf6N2E2_539 0.40 +0.6 607,883 + Pf6N2E2_539 0.40 +0.5 607,883 + Pf6N2E2_539 0.40 -0.1 607,883 + Pf6N2E2_539 0.40 +1.2 607,883 + Pf6N2E2_539 0.40 -0.4 607,884 - Pf6N2E2_539 0.41 +2.8 607,884 - Pf6N2E2_539 0.41 -0.4 607,884 - Pf6N2E2_539 0.41 +0.3 607,884 - Pf6N2E2_539 0.41 +0.2 607,884 - Pf6N2E2_539 0.41 -3.0 607,884 - Pf6N2E2_539 0.41 +0.7 607,884 - Pf6N2E2_539 0.41 -0.1 608,173 + Pf6N2E2_540 0.20 -0.2 608,173 + Pf6N2E2_540 0.20 +0.6 608,173 + Pf6N2E2_540 0.20 +0.0 608,173 + Pf6N2E2_540 0.20 -0.0 608,173 + Pf6N2E2_540 0.20 +1.1 608,173 + Pf6N2E2_540 0.20 +0.7 608,173 + Pf6N2E2_540 0.20 +0.2 608,174 - Pf6N2E2_540 0.20 +0.4 608,174 - Pf6N2E2_540 0.20 -0.1 608,174 - Pf6N2E2_540 0.20 +0.7 608,174 - Pf6N2E2_540 0.20 +0.8 608,174 - Pf6N2E2_540 0.20 -0.2 608,174 - Pf6N2E2_540 0.20 +1.4 608,174 - Pf6N2E2_540 0.20 +0.3 608,174 - Pf6N2E2_540 0.20 -0.5 608,174 - Pf6N2E2_540 0.20 +0.6 608,174 - Pf6N2E2_540 0.20 -0.4 608,596 + Pf6N2E2_540 0.68 -0.7 608,596 + Pf6N2E2_540 0.68 +0.2 608,597 - Pf6N2E2_540 0.69 +0.9 608,597 - Pf6N2E2_540 0.69 +0.3 608,985 + -0.4 608,985 + +2.4 608,986 - +0.7 608,986 - +0.7 609,015 - +1.2 609,017 - +1.2 609,220 + Pf6N2E2_541 0.36 -0.8 609,387 + Pf6N2E2_541 0.65 +0.8 609,388 - Pf6N2E2_541 0.66 +0.2 609,429 + Pf6N2E2_541 0.73 -0.8 609,429 + Pf6N2E2_541 0.73 -0.6 609,429 + Pf6N2E2_541 0.73 -0.4 609,430 - Pf6N2E2_541 0.73 +0.9 609,430 - Pf6N2E2_541 0.73 -0.7 609,430 - Pf6N2E2_541 0.73 +0.6 609,430 - Pf6N2E2_541 0.73 +0.6 609,430 - Pf6N2E2_541 0.73 -0.4 609,430 - Pf6N2E2_541 0.73 +0.8 609,430 - Pf6N2E2_541 0.73 +0.3 609,430 - Pf6N2E2_541 0.73 +2.0 609,430 - Pf6N2E2_541 0.73 +2.2 609,562 + +1.6 609,629 + +0.8 609,629 + +1.9 609,630 - -0.4 609,650 + -0.4 609,650 + -0.4 609,651 - -0.5 609,651 - +0.9 609,686 + +0.6 609,687 - +0.4 609,710 - +2.2 609,747 + +1.4 609,747 + +0.0 609,748 - -0.4 609,752 + -1.4 609,753 - +0.0 609,816 + +1.3
Or see this region's nucleotide sequence