Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_5091 and Pf6N2E2_5092 are separated by 89 nucleotides Pf6N2E2_5092 and Pf6N2E2_5093 are separated by 274 nucleotides Pf6N2E2_5093 and Pf6N2E2_5094 are separated by 408 nucleotides Pf6N2E2_5094 and Pf6N2E2_5095 are separated by 132 nucleotides
Pf6N2E2_5091 - putative outermembrane protein, at 5,873,119 to 5,874,972
_5091
Pf6N2E2_5092 - FIG00953225: hypothetical protein, at 5,875,062 to 5,875,550
_5092
Pf6N2E2_5093 - ATP-dependent protease La (EC 3.4.21.53) Type II, at 5,875,825 to 5,878,263
_5093
Pf6N2E2_5094 - FIG00956889: hypothetical protein, at 5,878,672 to 5,878,923
_5094
Pf6N2E2_5095 - Putative inner membrane protein (Fragment), at 5,879,056 to 5,879,544
_5095
Position (kb) 5875
5876
5877
5878
5879 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5874.972 kb on + strand at 5874.972 kb on + strand at 5874.973 kb on - strand at 5874.973 kb on - strand at 5874.981 kb on - strand at 5875.064 kb on - strand at 5875.064 kb on - strand at 5875.064 kb on - strand at 5875.064 kb on - strand at 5875.064 kb on - strand at 5875.079 kb on - strand at 5875.079 kb on - strand at 5875.124 kb on + strand, within Pf6N2E2_5092 at 5875.124 kb on + strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.125 kb on - strand, within Pf6N2E2_5092 at 5875.237 kb on + strand, within Pf6N2E2_5092 at 5875.237 kb on + strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.238 kb on - strand, within Pf6N2E2_5092 at 5875.328 kb on - strand, within Pf6N2E2_5092 at 5875.382 kb on + strand, within Pf6N2E2_5092 at 5875.383 kb on - strand, within Pf6N2E2_5092 at 5875.383 kb on - strand, within Pf6N2E2_5092 at 5875.512 kb on + strand at 5875.550 kb on + strand at 5875.550 kb on + strand at 5875.550 kb on + strand at 5875.550 kb on + strand at 5875.551 kb on - strand at 5875.551 kb on - strand at 5875.551 kb on - strand at 5875.551 kb on - strand at 5875.551 kb on - strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.554 kb on + strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.555 kb on - strand at 5875.565 kb on + strand at 5875.565 kb on + strand at 5875.565 kb on + strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.566 kb on - strand at 5875.597 kb on + strand at 5875.598 kb on - strand at 5875.646 kb on + strand at 5875.646 kb on + strand at 5875.738 kb on - strand at 5875.738 kb on - strand at 5875.773 kb on - strand at 5875.796 kb on - strand at 5875.796 kb on - strand at 5875.813 kb on + strand at 5875.813 kb on + strand at 5875.813 kb on + strand at 5875.813 kb on + strand at 5875.813 kb on + strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.814 kb on - strand at 5875.908 kb on + strand at 5875.909 kb on - strand at 5876.002 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.009 kb on + strand at 5876.010 kb on - strand at 5876.010 kb on - strand at 5876.010 kb on - strand at 5876.010 kb on - strand at 5876.059 kb on + strand at 5876.107 kb on + strand, within Pf6N2E2_5093 at 5876.108 kb on - strand, within Pf6N2E2_5093 at 5876.108 kb on - strand, within Pf6N2E2_5093 at 5876.164 kb on - strand, within Pf6N2E2_5093 at 5876.248 kb on + strand, within Pf6N2E2_5093 at 5876.249 kb on - strand, within Pf6N2E2_5093 at 5876.249 kb on - strand, within Pf6N2E2_5093 at 5876.249 kb on - strand, within Pf6N2E2_5093 at 5876.293 kb on + strand, within Pf6N2E2_5093 at 5876.294 kb on - strand, within Pf6N2E2_5093 at 5876.294 kb on - strand, within Pf6N2E2_5093 at 5876.294 kb on - strand, within Pf6N2E2_5093 at 5876.351 kb on - strand, within Pf6N2E2_5093 at 5876.351 kb on - strand, within Pf6N2E2_5093 at 5876.605 kb on + strand, within Pf6N2E2_5093 at 5876.606 kb on - strand, within Pf6N2E2_5093 at 5876.653 kb on + strand, within Pf6N2E2_5093 at 5876.653 kb on + strand, within Pf6N2E2_5093 at 5876.653 kb on + strand, within Pf6N2E2_5093 at 5876.654 kb on - strand, within Pf6N2E2_5093 at 5876.654 kb on - strand, within Pf6N2E2_5093 at 5876.654 kb on - strand, within Pf6N2E2_5093 at 5876.734 kb on + strand, within Pf6N2E2_5093 at 5876.735 kb on - strand, within Pf6N2E2_5093 at 5876.735 kb on - strand, within Pf6N2E2_5093 at 5876.735 kb on - strand, within Pf6N2E2_5093 at 5876.735 kb on - strand, within Pf6N2E2_5093 at 5876.833 kb on + strand, within Pf6N2E2_5093 at 5876.833 kb on + strand, within Pf6N2E2_5093 at 5876.833 kb on + strand, within Pf6N2E2_5093 at 5876.834 kb on - strand, within Pf6N2E2_5093 at 5876.857 kb on + strand, within Pf6N2E2_5093 at 5876.858 kb on - strand, within Pf6N2E2_5093 at 5876.858 kb on - strand, within Pf6N2E2_5093 at 5876.866 kb on + strand, within Pf6N2E2_5093 at 5876.867 kb on - strand, within Pf6N2E2_5093 at 5877.103 kb on + strand, within Pf6N2E2_5093 at 5877.103 kb on + strand, within Pf6N2E2_5093 at 5877.103 kb on + strand, within Pf6N2E2_5093 at 5877.103 kb on + strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.104 kb on - strand, within Pf6N2E2_5093 at 5877.106 kb on + strand, within Pf6N2E2_5093 at 5877.106 kb on + strand, within Pf6N2E2_5093 at 5877.106 kb on + strand, within Pf6N2E2_5093 at 5877.106 kb on + strand, within Pf6N2E2_5093 at 5877.107 kb on - strand, within Pf6N2E2_5093 at 5877.107 kb on - strand, within Pf6N2E2_5093 at 5877.107 kb on - strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.286 kb on + strand, within Pf6N2E2_5093 at 5877.287 kb on - strand, within Pf6N2E2_5093 at 5877.287 kb on - strand, within Pf6N2E2_5093 at 5877.287 kb on - strand, within Pf6N2E2_5093 at 5877.287 kb on - strand, within Pf6N2E2_5093 at 5877.411 kb on + strand, within Pf6N2E2_5093 at 5877.545 kb on + strand, within Pf6N2E2_5093 at 5877.545 kb on + strand, within Pf6N2E2_5093 at 5877.545 kb on + strand, within Pf6N2E2_5093 at 5877.546 kb on - strand, within Pf6N2E2_5093 at 5877.684 kb on + strand, within Pf6N2E2_5093 at 5877.760 kb on + strand, within Pf6N2E2_5093 at 5877.760 kb on + strand, within Pf6N2E2_5093 at 5877.761 kb on - strand, within Pf6N2E2_5093 at 5877.761 kb on - strand, within Pf6N2E2_5093 at 5877.761 kb on - strand, within Pf6N2E2_5093 at 5877.766 kb on + strand, within Pf6N2E2_5093 at 5877.804 kb on + strand, within Pf6N2E2_5093 at 5877.805 kb on - strand, within Pf6N2E2_5093 at 5878.051 kb on + strand at 5878.051 kb on + strand at 5878.051 kb on + strand at 5878.051 kb on + strand at 5878.052 kb on - strand at 5878.379 kb on - strand at 5878.393 kb on + strand at 5878.492 kb on + strand at 5878.565 kb on + strand at 5878.566 kb on - strand at 5878.566 kb on - strand at 5878.566 kb on - strand at 5878.665 kb on + strand at 5878.666 kb on - strand at 5878.671 kb on + strand at 5878.776 kb on + strand, within Pf6N2E2_5094 at 5878.807 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.832 kb on + strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.833 kb on - strand, within Pf6N2E2_5094 at 5878.951 kb on + strand at 5878.952 kb on - strand at 5879.017 kb on + strand at 5879.017 kb on + strand at 5879.017 kb on + strand at 5879.017 kb on + strand at 5879.018 kb on - strand at 5879.018 kb on - strand at 5879.018 kb on - strand at 5879.018 kb on - strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.019 kb on + strand at 5879.020 kb on - strand at 5879.020 kb on - strand at 5879.020 kb on - strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.083 kb on + strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.084 kb on - strand at 5879.113 kb on + strand, within Pf6N2E2_5095
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,874,972 + -1.0 5,874,972 + +0.2 5,874,973 - -0.7 5,874,973 - +0.9 5,874,981 - +0.3 5,875,064 - +0.1 5,875,064 - -0.3 5,875,064 - +0.3 5,875,064 - +0.3 5,875,064 - -0.7 5,875,079 - +0.3 5,875,079 - +0.3 5,875,124 + Pf6N2E2_5092 0.13 +0.5 5,875,124 + Pf6N2E2_5092 0.13 +0.7 5,875,125 - Pf6N2E2_5092 0.13 +1.9 5,875,125 - Pf6N2E2_5092 0.13 +0.3 5,875,125 - Pf6N2E2_5092 0.13 -1.3 5,875,125 - Pf6N2E2_5092 0.13 -0.1 5,875,125 - Pf6N2E2_5092 0.13 +0.3 5,875,237 + Pf6N2E2_5092 0.36 +1.9 5,875,237 + Pf6N2E2_5092 0.36 +0.3 5,875,238 - Pf6N2E2_5092 0.36 +0.3 5,875,238 - Pf6N2E2_5092 0.36 +1.3 5,875,238 - Pf6N2E2_5092 0.36 +1.0 5,875,238 - Pf6N2E2_5092 0.36 +0.7 5,875,238 - Pf6N2E2_5092 0.36 +1.9 5,875,328 - Pf6N2E2_5092 0.54 +1.3 5,875,382 + Pf6N2E2_5092 0.65 -1.1 5,875,383 - Pf6N2E2_5092 0.66 +0.3 5,875,383 - Pf6N2E2_5092 0.66 +0.3 5,875,512 + +0.8 5,875,550 + +0.9 5,875,550 + -1.5 5,875,550 + +0.3 5,875,550 + +0.9 5,875,551 - +0.3 5,875,551 - +1.0 5,875,551 - +0.3 5,875,551 - +0.3 5,875,551 - +0.3 5,875,554 + +0.3 5,875,554 + -0.3 5,875,554 + +1.9 5,875,554 + +1.3 5,875,554 + +0.7 5,875,554 + -1.0 5,875,554 + +0.7 5,875,554 + +0.3 5,875,554 + -0.2 5,875,554 + +0.6 5,875,554 + -0.7 5,875,554 + +0.4 5,875,555 - -0.4 5,875,555 - -1.3 5,875,555 - +0.3 5,875,555 - -0.7 5,875,555 - +0.3 5,875,555 - +1.3 5,875,555 - +1.3 5,875,555 - -0.7 5,875,555 - +0.3 5,875,555 - +3.1 5,875,565 + +1.3 5,875,565 + -0.5 5,875,565 + +0.3 5,875,566 - -0.2 5,875,566 - -0.4 5,875,566 - +0.7 5,875,566 - -2.0 5,875,566 - +0.1 5,875,566 - +0.8 5,875,566 - +0.7 5,875,597 + +0.3 5,875,598 - +0.3 5,875,646 + +2.0 5,875,646 + +0.5 5,875,738 - +1.0 5,875,738 - +0.4 5,875,773 - -0.2 5,875,796 - -0.4 5,875,796 - -0.5 5,875,813 + +0.7 5,875,813 + -0.3 5,875,813 + -0.6 5,875,813 + -0.1 5,875,813 + +0.4 5,875,814 - -0.3 5,875,814 - +1.1 5,875,814 - -0.7 5,875,814 - -0.9 5,875,814 - -0.7 5,875,814 - -1.1 5,875,814 - -0.4 5,875,814 - -1.5 5,875,814 - -0.2 5,875,814 - -0.3 5,875,814 - -1.6 5,875,814 - -1.1 5,875,908 + -0.1 5,875,909 - -0.8 5,876,002 + +0.3 5,876,009 + -0.1 5,876,009 + -0.7 5,876,009 + -0.9 5,876,009 + +0.3 5,876,009 + +0.3 5,876,009 + +0.3 5,876,009 + +0.3 5,876,010 - -1.0 5,876,010 - -0.7 5,876,010 - -0.3 5,876,010 - +0.5 5,876,059 + -1.0 5,876,107 + Pf6N2E2_5093 0.12 +1.0 5,876,108 - Pf6N2E2_5093 0.12 -1.5 5,876,108 - Pf6N2E2_5093 0.12 -0.4 5,876,164 - Pf6N2E2_5093 0.14 -0.4 5,876,248 + Pf6N2E2_5093 0.17 -1.9 5,876,249 - Pf6N2E2_5093 0.17 +0.1 5,876,249 - Pf6N2E2_5093 0.17 -0.2 5,876,249 - Pf6N2E2_5093 0.17 +0.9 5,876,293 + Pf6N2E2_5093 0.19 -0.5 5,876,294 - Pf6N2E2_5093 0.19 -0.8 5,876,294 - Pf6N2E2_5093 0.19 +0.3 5,876,294 - Pf6N2E2_5093 0.19 -2.3 5,876,351 - Pf6N2E2_5093 0.22 +0.1 5,876,351 - Pf6N2E2_5093 0.22 -0.7 5,876,605 + Pf6N2E2_5093 0.32 -1.0 5,876,606 - Pf6N2E2_5093 0.32 -1.7 5,876,653 + Pf6N2E2_5093 0.34 -1.8 5,876,653 + Pf6N2E2_5093 0.34 -0.5 5,876,653 + Pf6N2E2_5093 0.34 -0.8 5,876,654 - Pf6N2E2_5093 0.34 -1.7 5,876,654 - Pf6N2E2_5093 0.34 -1.3 5,876,654 - Pf6N2E2_5093 0.34 +0.3 5,876,734 + Pf6N2E2_5093 0.37 -0.9 5,876,735 - Pf6N2E2_5093 0.37 -0.3 5,876,735 - Pf6N2E2_5093 0.37 -0.5 5,876,735 - Pf6N2E2_5093 0.37 -0.7 5,876,735 - Pf6N2E2_5093 0.37 +0.3 5,876,833 + Pf6N2E2_5093 0.41 -0.1 5,876,833 + Pf6N2E2_5093 0.41 -1.5 5,876,833 + Pf6N2E2_5093 0.41 -0.5 5,876,834 - Pf6N2E2_5093 0.41 -1.3 5,876,857 + Pf6N2E2_5093 0.42 -1.0 5,876,858 - Pf6N2E2_5093 0.42 -2.3 5,876,858 - Pf6N2E2_5093 0.42 -1.7 5,876,866 + Pf6N2E2_5093 0.43 -1.3 5,876,867 - Pf6N2E2_5093 0.43 -0.3 5,877,103 + Pf6N2E2_5093 0.52 -0.7 5,877,103 + Pf6N2E2_5093 0.52 -1.4 5,877,103 + Pf6N2E2_5093 0.52 +0.3 5,877,103 + Pf6N2E2_5093 0.52 -2.2 5,877,104 - Pf6N2E2_5093 0.52 -3.4 5,877,104 - Pf6N2E2_5093 0.52 -0.9 5,877,104 - Pf6N2E2_5093 0.52 -0.5 5,877,104 - Pf6N2E2_5093 0.52 -1.1 5,877,104 - Pf6N2E2_5093 0.52 -0.2 5,877,106 + Pf6N2E2_5093 0.53 -0.5 5,877,106 + Pf6N2E2_5093 0.53 +0.9 5,877,106 + Pf6N2E2_5093 0.53 -1.8 5,877,106 + Pf6N2E2_5093 0.53 -1.0 5,877,107 - Pf6N2E2_5093 0.53 -0.3 5,877,107 - Pf6N2E2_5093 0.53 -0.6 5,877,107 - Pf6N2E2_5093 0.53 -2.0 5,877,286 + Pf6N2E2_5093 0.60 +0.3 5,877,286 + Pf6N2E2_5093 0.60 -1.7 5,877,286 + Pf6N2E2_5093 0.60 -0.7 5,877,286 + Pf6N2E2_5093 0.60 -0.9 5,877,286 + Pf6N2E2_5093 0.60 -0.5 5,877,287 - Pf6N2E2_5093 0.60 -1.1 5,877,287 - Pf6N2E2_5093 0.60 -0.3 5,877,287 - Pf6N2E2_5093 0.60 -1.0 5,877,287 - Pf6N2E2_5093 0.60 -0.7 5,877,411 + Pf6N2E2_5093 0.65 -0.6 5,877,545 + Pf6N2E2_5093 0.71 -0.0 5,877,545 + Pf6N2E2_5093 0.71 -0.1 5,877,545 + Pf6N2E2_5093 0.71 -1.4 5,877,546 - Pf6N2E2_5093 0.71 -0.7 5,877,684 + Pf6N2E2_5093 0.76 -0.8 5,877,760 + Pf6N2E2_5093 0.79 -0.2 5,877,760 + Pf6N2E2_5093 0.79 +0.3 5,877,761 - Pf6N2E2_5093 0.79 -1.7 5,877,761 - Pf6N2E2_5093 0.79 -0.0 5,877,761 - Pf6N2E2_5093 0.79 +0.5 5,877,766 + Pf6N2E2_5093 0.80 +0.3 5,877,804 + Pf6N2E2_5093 0.81 -0.3 5,877,805 - Pf6N2E2_5093 0.81 +1.3 5,878,051 + +0.3 5,878,051 + -1.4 5,878,051 + -3.2 5,878,051 + +0.3 5,878,052 - -0.7 5,878,379 - -1.6 5,878,393 + +0.3 5,878,492 + +0.3 5,878,565 + -0.7 5,878,566 - +0.3 5,878,566 - +1.1 5,878,566 - -0.0 5,878,665 + +0.3 5,878,666 - +0.4 5,878,671 + +0.3 5,878,776 + Pf6N2E2_5094 0.41 -0.1 5,878,807 + Pf6N2E2_5094 0.54 +0.3 5,878,832 + Pf6N2E2_5094 0.63 +0.9 5,878,832 + Pf6N2E2_5094 0.63 +0.9 5,878,832 + Pf6N2E2_5094 0.63 -0.5 5,878,832 + Pf6N2E2_5094 0.63 +0.9 5,878,832 + Pf6N2E2_5094 0.63 +0.5 5,878,832 + Pf6N2E2_5094 0.63 +0.3 5,878,833 - Pf6N2E2_5094 0.64 -0.7 5,878,833 - Pf6N2E2_5094 0.64 +0.7 5,878,833 - Pf6N2E2_5094 0.64 +1.9 5,878,833 - Pf6N2E2_5094 0.64 -1.7 5,878,833 - Pf6N2E2_5094 0.64 +0.0 5,878,833 - Pf6N2E2_5094 0.64 -0.3 5,878,951 + +1.4 5,878,952 - +1.0 5,879,017 + +0.3 5,879,017 + +0.3 5,879,017 + -0.1 5,879,017 + +1.7 5,879,018 - -2.0 5,879,018 - +0.6 5,879,018 - -0.7 5,879,018 - -1.5 5,879,019 + +0.8 5,879,019 + +0.1 5,879,019 + -0.6 5,879,019 + +0.9 5,879,019 + +0.3 5,879,019 + +0.7 5,879,020 - -0.7 5,879,020 - +2.6 5,879,020 - -0.1 5,879,083 + +1.4 5,879,083 + +0.3 5,879,083 + -0.3 5,879,083 + +1.0 5,879,083 + +0.3 5,879,083 + +0.3 5,879,083 + -0.3 5,879,083 + +0.3 5,879,084 - -0.4 5,879,084 - -0.7 5,879,084 - -0.1 5,879,084 - +1.3 5,879,084 - +1.7 5,879,084 - -0.1 5,879,113 + Pf6N2E2_5095 0.12 +0.1
Or see this region's nucleotide sequence