Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4963 and Pf6N2E2_4964 overlap by 4 nucleotides Pf6N2E2_4964 and Pf6N2E2_4965 are separated by 112 nucleotides
Pf6N2E2_4963 - Homoserine dehydrogenase (EC 1.1.1.3), at 5,724,211 to 5,725,269
_4963
Pf6N2E2_4964 - Aldehyde dehydrogenase (EC 1.2.1.3), at 5,725,266 to 5,726,678
_4964
Pf6N2E2_4965 - hypothetical protein, at 5,726,791 to 5,727,738
_4965
Position (kb) 5725
5726
5727 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5724.589 kb on - strand, within Pf6N2E2_4963 at 5724.662 kb on - strand, within Pf6N2E2_4963 at 5725.408 kb on + strand, within Pf6N2E2_4964 at 5725.409 kb on - strand, within Pf6N2E2_4964 at 5725.409 kb on - strand, within Pf6N2E2_4964 at 5725.705 kb on - strand, within Pf6N2E2_4964 at 5725.705 kb on - strand, within Pf6N2E2_4964 at 5726.133 kb on + strand, within Pf6N2E2_4964 at 5726.133 kb on + strand, within Pf6N2E2_4964 at 5726.133 kb on + strand, within Pf6N2E2_4964 at 5726.134 kb on - strand, within Pf6N2E2_4964 at 5726.134 kb on - strand, within Pf6N2E2_4964 at 5726.290 kb on + strand, within Pf6N2E2_4964 at 5726.290 kb on + strand, within Pf6N2E2_4964 at 5726.290 kb on + strand, within Pf6N2E2_4964 at 5726.290 kb on + strand, within Pf6N2E2_4964 at 5726.290 kb on + strand, within Pf6N2E2_4964 at 5726.291 kb on - strand, within Pf6N2E2_4964 at 5726.291 kb on - strand, within Pf6N2E2_4964 at 5726.291 kb on - strand, within Pf6N2E2_4964 at 5726.291 kb on - strand, within Pf6N2E2_4964 at 5726.291 kb on - strand, within Pf6N2E2_4964 at 5726.322 kb on + strand, within Pf6N2E2_4964 at 5726.322 kb on + strand, within Pf6N2E2_4964 at 5726.478 kb on - strand, within Pf6N2E2_4964 at 5726.592 kb on + strand at 5726.593 kb on - strand at 5726.628 kb on + strand at 5726.666 kb on + strand at 5726.666 kb on + strand at 5726.666 kb on + strand at 5726.666 kb on + strand at 5726.666 kb on + strand at 5726.667 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.773 kb on - strand at 5726.793 kb on - strand at 5726.793 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,724,589 - Pf6N2E2_4963 0.36 +0.3 5,724,662 - Pf6N2E2_4963 0.43 -0.5 5,725,408 + Pf6N2E2_4964 0.10 -0.3 5,725,409 - Pf6N2E2_4964 0.10 -0.1 5,725,409 - Pf6N2E2_4964 0.10 -0.3 5,725,705 - Pf6N2E2_4964 0.31 +0.3 5,725,705 - Pf6N2E2_4964 0.31 +1.6 5,726,133 + Pf6N2E2_4964 0.61 +1.7 5,726,133 + Pf6N2E2_4964 0.61 -0.7 5,726,133 + Pf6N2E2_4964 0.61 +0.8 5,726,134 - Pf6N2E2_4964 0.61 -1.3 5,726,134 - Pf6N2E2_4964 0.61 +0.6 5,726,290 + Pf6N2E2_4964 0.72 -0.2 5,726,290 + Pf6N2E2_4964 0.72 +0.4 5,726,290 + Pf6N2E2_4964 0.72 -0.7 5,726,290 + Pf6N2E2_4964 0.72 +0.9 5,726,290 + Pf6N2E2_4964 0.72 -0.0 5,726,291 - Pf6N2E2_4964 0.73 -0.3 5,726,291 - Pf6N2E2_4964 0.73 +1.0 5,726,291 - Pf6N2E2_4964 0.73 +1.0 5,726,291 - Pf6N2E2_4964 0.73 +0.3 5,726,291 - Pf6N2E2_4964 0.73 +2.3 5,726,322 + Pf6N2E2_4964 0.75 -0.2 5,726,322 + Pf6N2E2_4964 0.75 -0.3 5,726,478 - Pf6N2E2_4964 0.86 -1.9 5,726,592 + -0.9 5,726,593 - +0.3 5,726,628 + +0.3 5,726,666 + -0.3 5,726,666 + -0.0 5,726,666 + -1.3 5,726,666 + -0.3 5,726,666 + +0.1 5,726,667 - -1.3 5,726,773 - +0.7 5,726,773 - +0.6 5,726,773 - -0.0 5,726,773 - +0.4 5,726,773 - +0.3 5,726,773 - +1.0 5,726,773 - +0.4 5,726,773 - -1.3 5,726,773 - +0.1 5,726,773 - +0.1 5,726,793 - +0.6 5,726,793 - +1.1
Or see this region's nucleotide sequence