Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4611 and Pf6N2E2_4612 are separated by 77 nucleotides Pf6N2E2_4612 and Pf6N2E2_4613 are separated by 152 nucleotides Pf6N2E2_4613 and Pf6N2E2_4614 are separated by 48 nucleotides
Pf6N2E2_4611 - Ferric siderophore transport system, periplasmic binding protein TonB, at 5,335,924 to 5,336,820
_4611
Pf6N2E2_4612 - Glutathione synthetase (EC 6.3.2.3), at 5,336,898 to 5,337,851
_4612
Pf6N2E2_4613 - twitching motility protein PilG, at 5,338,004 to 5,338,504
_4613
Pf6N2E2_4614 - twitching motility protein PilH, at 5,338,553 to 5,338,918
_4614
Position (kb) 5336
5337
5338 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5335.911 kb on + strand at 5335.911 kb on + strand at 5335.912 kb on - strand at 5335.912 kb on - strand at 5335.912 kb on - strand at 5336.083 kb on + strand, within Pf6N2E2_4611 at 5336.083 kb on + strand, within Pf6N2E2_4611 at 5336.099 kb on + strand, within Pf6N2E2_4611 at 5336.099 kb on + strand, within Pf6N2E2_4611 at 5336.100 kb on - strand, within Pf6N2E2_4611 at 5336.148 kb on - strand, within Pf6N2E2_4611 at 5336.291 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.517 kb on + strand, within Pf6N2E2_4611 at 5336.863 kb on + strand at 5337.188 kb on - strand, within Pf6N2E2_4612 at 5337.188 kb on - strand, within Pf6N2E2_4612 at 5337.250 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.550 kb on + strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.551 kb on - strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.559 kb on + strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.740 kb on - strand, within Pf6N2E2_4612 at 5337.792 kb on - strand at 5337.800 kb on + strand at 5337.803 kb on - strand at 5337.843 kb on + strand at 5337.844 kb on - strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.882 kb on + strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.883 kb on - strand at 5337.984 kb on + strand at 5338.047 kb on + strand at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.595 kb on + strand, within Pf6N2E2_4614 at 5338.596 kb on - strand, within Pf6N2E2_4614
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,335,911 + +1.2 5,335,911 + -0.2 5,335,912 - +1.5 5,335,912 - +0.4 5,335,912 - +2.0 5,336,083 + Pf6N2E2_4611 0.18 +0.4 5,336,083 + Pf6N2E2_4611 0.18 +0.4 5,336,099 + Pf6N2E2_4611 0.20 +0.4 5,336,099 + Pf6N2E2_4611 0.20 +0.4 5,336,100 - Pf6N2E2_4611 0.20 +0.4 5,336,148 - Pf6N2E2_4611 0.25 -0.1 5,336,291 + Pf6N2E2_4611 0.41 +0.4 5,336,517 + Pf6N2E2_4611 0.66 +0.4 5,336,517 + Pf6N2E2_4611 0.66 +0.4 5,336,517 + Pf6N2E2_4611 0.66 +0.4 5,336,517 + Pf6N2E2_4611 0.66 +0.4 5,336,517 + Pf6N2E2_4611 0.66 +0.4 5,336,517 + Pf6N2E2_4611 0.66 +0.4 5,336,863 + +0.5 5,337,188 - Pf6N2E2_4612 0.30 +0.4 5,337,188 - Pf6N2E2_4612 0.30 +0.4 5,337,250 + Pf6N2E2_4612 0.37 +0.4 5,337,550 + Pf6N2E2_4612 0.68 +0.4 5,337,550 + Pf6N2E2_4612 0.68 +0.4 5,337,550 + Pf6N2E2_4612 0.68 +0.4 5,337,550 + Pf6N2E2_4612 0.68 +0.4 5,337,550 + Pf6N2E2_4612 0.68 +0.4 5,337,551 - Pf6N2E2_4612 0.68 +0.4 5,337,551 - Pf6N2E2_4612 0.68 +0.4 5,337,551 - Pf6N2E2_4612 0.68 -1.9 5,337,559 + Pf6N2E2_4612 0.69 +0.4 5,337,559 + Pf6N2E2_4612 0.69 +0.4 5,337,740 - Pf6N2E2_4612 0.88 +0.4 5,337,740 - Pf6N2E2_4612 0.88 +0.4 5,337,792 - +0.4 5,337,800 + +0.4 5,337,803 - +0.4 5,337,843 + +0.4 5,337,844 - +0.4 5,337,882 + +1.0 5,337,882 + +0.4 5,337,882 + +0.4 5,337,882 + +1.4 5,337,882 + -0.1 5,337,883 - +0.4 5,337,883 - +0.7 5,337,883 - -0.0 5,337,883 - +1.8 5,337,883 - +1.2 5,337,883 - +2.0 5,337,883 - +0.6 5,337,883 - +0.4 5,337,883 - -0.8 5,337,984 + +0.0 5,338,047 + +0.5 5,338,595 + Pf6N2E2_4614 0.11 -2.0 5,338,595 + Pf6N2E2_4614 0.11 -2.0 5,338,595 + Pf6N2E2_4614 0.11 +0.4 5,338,596 - Pf6N2E2_4614 0.12 -0.6
Or see this region's nucleotide sequence