Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4434 and Pf6N2E2_4435 are separated by 174 nucleotides Pf6N2E2_4435 and Pf6N2E2_4436 are separated by 59 nucleotides Pf6N2E2_4436 and Pf6N2E2_4437 are separated by 191 nucleotides
Pf6N2E2_4434 - Periplasmic thiol:disulfide oxidoreductase DsbB, required for DsbA reoxidation, at 5,166,850 to 5,167,392
_4434
Pf6N2E2_4435 - Regulator of sigma D, at 5,167,567 to 5,168,025
_4435
Pf6N2E2_4436 - Macrophage infectivity potentiator, at 5,168,085 to 5,168,750
_4436
Pf6N2E2_4437 - Twin-arginine translocation protein TatB, at 5,168,942 to 5,170,117
_4437
Position (kb) 5167
5168
5169 Strain fitness (log2 ratio) -1
0
1
2
3 at 5166.588 kb on - strand at 5166.614 kb on + strand at 5166.615 kb on - strand at 5166.703 kb on - strand at 5166.711 kb on - strand at 5166.728 kb on - strand at 5166.755 kb on + strand at 5166.755 kb on + strand at 5166.756 kb on - strand at 5166.756 kb on - strand at 5166.818 kb on + strand at 5166.839 kb on - strand at 5166.839 kb on - strand at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.925 kb on + strand, within Pf6N2E2_4434 at 5166.934 kb on + strand, within Pf6N2E2_4434 at 5166.934 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.998 kb on + strand, within Pf6N2E2_4434 at 5166.999 kb on - strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.022 kb on + strand, within Pf6N2E2_4434 at 5167.023 kb on - strand, within Pf6N2E2_4434 at 5167.023 kb on - strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.054 kb on + strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.055 kb on - strand, within Pf6N2E2_4434 at 5167.230 kb on + strand, within Pf6N2E2_4434 at 5167.231 kb on - strand, within Pf6N2E2_4434 at 5167.257 kb on + strand, within Pf6N2E2_4434 at 5167.309 kb on + strand, within Pf6N2E2_4434 at 5167.309 kb on + strand, within Pf6N2E2_4434 at 5167.310 kb on - strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.313 kb on + strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.314 kb on - strand, within Pf6N2E2_4434 at 5167.363 kb on - strand at 5167.400 kb on + strand at 5167.400 kb on + strand at 5167.400 kb on + strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.401 kb on - strand at 5167.409 kb on + strand at 5167.410 kb on - strand at 5167.410 kb on - strand at 5167.420 kb on + strand at 5167.420 kb on + strand at 5167.421 kb on - strand at 5167.447 kb on + strand at 5167.447 kb on + strand at 5167.664 kb on - strand, within Pf6N2E2_4435 at 5167.681 kb on - strand, within Pf6N2E2_4435 at 5167.744 kb on + strand, within Pf6N2E2_4435 at 5167.744 kb on + strand, within Pf6N2E2_4435 at 5167.744 kb on + strand, within Pf6N2E2_4435 at 5167.744 kb on + strand, within Pf6N2E2_4435 at 5167.744 kb on + strand, within Pf6N2E2_4435 at 5167.744 kb on + strand, within Pf6N2E2_4435 at 5167.745 kb on - strand, within Pf6N2E2_4435 at 5167.745 kb on - strand, within Pf6N2E2_4435 at 5167.745 kb on - strand, within Pf6N2E2_4435 at 5167.745 kb on - strand, within Pf6N2E2_4435 at 5167.745 kb on - strand, within Pf6N2E2_4435 at 5167.745 kb on - strand, within Pf6N2E2_4435 at 5167.769 kb on + strand, within Pf6N2E2_4435 at 5167.769 kb on + strand, within Pf6N2E2_4435 at 5167.769 kb on + strand, within Pf6N2E2_4435 at 5167.770 kb on - strand, within Pf6N2E2_4435 at 5167.770 kb on - strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.771 kb on + strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.772 kb on - strand, within Pf6N2E2_4435 at 5167.840 kb on + strand, within Pf6N2E2_4435 at 5167.841 kb on - strand, within Pf6N2E2_4435 at 5168.167 kb on + strand, within Pf6N2E2_4436 at 5168.167 kb on + strand, within Pf6N2E2_4436 at 5168.319 kb on - strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.320 kb on + strand, within Pf6N2E2_4436 at 5168.321 kb on - strand, within Pf6N2E2_4436 at 5168.321 kb on - strand, within Pf6N2E2_4436 at 5168.321 kb on - strand, within Pf6N2E2_4436 at 5168.321 kb on - strand, within Pf6N2E2_4436 at 5168.321 kb on - strand, within Pf6N2E2_4436 at 5168.566 kb on + strand, within Pf6N2E2_4436 at 5168.566 kb on + strand, within Pf6N2E2_4436 at 5168.567 kb on - strand, within Pf6N2E2_4436 at 5168.567 kb on - strand, within Pf6N2E2_4436 at 5168.567 kb on - strand, within Pf6N2E2_4436 at 5168.567 kb on - strand, within Pf6N2E2_4436 at 5168.656 kb on + strand, within Pf6N2E2_4436 at 5168.657 kb on - strand, within Pf6N2E2_4436 at 5168.657 kb on - strand, within Pf6N2E2_4436 at 5168.657 kb on - strand, within Pf6N2E2_4436 at 5168.731 kb on - strand at 5168.780 kb on + strand at 5168.780 kb on + strand at 5168.781 kb on - strand at 5168.781 kb on - strand at 5168.781 kb on - strand at 5168.781 kb on - strand at 5168.865 kb on + strand at 5168.865 kb on + strand at 5168.865 kb on + strand at 5168.866 kb on - strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.904 kb on + strand at 5168.905 kb on - strand at 5168.905 kb on - strand at 5168.905 kb on - strand at 5168.905 kb on - strand at 5168.905 kb on - strand at 5168.907 kb on + strand at 5168.907 kb on + strand at 5168.907 kb on + strand at 5168.907 kb on + strand at 5168.907 kb on + strand at 5168.908 kb on - strand at 5168.908 kb on - strand at 5168.908 kb on - strand at 5168.908 kb on - strand at 5168.936 kb on + strand at 5168.937 kb on - strand at 5168.937 kb on - strand at 5168.937 kb on - strand at 5168.964 kb on + strand at 5168.965 kb on - strand at 5168.965 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,166,588 - +0.4 5,166,614 + +0.1 5,166,615 - +0.0 5,166,703 - -0.4 5,166,711 - +0.2 5,166,728 - +0.8 5,166,755 + +0.8 5,166,755 + +1.1 5,166,756 - -0.5 5,166,756 - +0.8 5,166,818 + +0.6 5,166,839 - +0.2 5,166,839 - +0.4 5,166,925 + Pf6N2E2_4434 0.14 +0.4 5,166,925 + Pf6N2E2_4434 0.14 +0.4 5,166,925 + Pf6N2E2_4434 0.14 +0.6 5,166,934 + Pf6N2E2_4434 0.15 -0.6 5,166,934 + Pf6N2E2_4434 0.15 -0.2 5,166,998 + Pf6N2E2_4434 0.27 +0.2 5,166,998 + Pf6N2E2_4434 0.27 -0.6 5,166,998 + Pf6N2E2_4434 0.27 +1.7 5,166,998 + Pf6N2E2_4434 0.27 +0.2 5,166,998 + Pf6N2E2_4434 0.27 +1.1 5,166,998 + Pf6N2E2_4434 0.27 +0.6 5,166,999 - Pf6N2E2_4434 0.27 +1.4 5,167,022 + Pf6N2E2_4434 0.32 +0.6 5,167,022 + Pf6N2E2_4434 0.32 +0.2 5,167,022 + Pf6N2E2_4434 0.32 +0.4 5,167,022 + Pf6N2E2_4434 0.32 +0.2 5,167,022 + Pf6N2E2_4434 0.32 +0.9 5,167,022 + Pf6N2E2_4434 0.32 -1.2 5,167,022 + Pf6N2E2_4434 0.32 -1.4 5,167,023 - Pf6N2E2_4434 0.32 +3.2 5,167,023 - Pf6N2E2_4434 0.32 +0.2 5,167,054 + Pf6N2E2_4434 0.38 -0.7 5,167,054 + Pf6N2E2_4434 0.38 +0.4 5,167,054 + Pf6N2E2_4434 0.38 +0.9 5,167,054 + Pf6N2E2_4434 0.38 +0.4 5,167,054 + Pf6N2E2_4434 0.38 +0.4 5,167,055 - Pf6N2E2_4434 0.38 -0.3 5,167,055 - Pf6N2E2_4434 0.38 -0.1 5,167,055 - Pf6N2E2_4434 0.38 +0.8 5,167,230 + Pf6N2E2_4434 0.70 +0.9 5,167,231 - Pf6N2E2_4434 0.70 +1.4 5,167,257 + Pf6N2E2_4434 0.75 +0.7 5,167,309 + Pf6N2E2_4434 0.85 -0.6 5,167,309 + Pf6N2E2_4434 0.85 +0.2 5,167,310 - Pf6N2E2_4434 0.85 +1.4 5,167,313 + Pf6N2E2_4434 0.85 -0.2 5,167,313 + Pf6N2E2_4434 0.85 -0.4 5,167,313 + Pf6N2E2_4434 0.85 +0.4 5,167,313 + Pf6N2E2_4434 0.85 +0.5 5,167,313 + Pf6N2E2_4434 0.85 +0.5 5,167,313 + Pf6N2E2_4434 0.85 -0.3 5,167,313 + Pf6N2E2_4434 0.85 +2.7 5,167,314 - Pf6N2E2_4434 0.85 +0.9 5,167,314 - Pf6N2E2_4434 0.85 +2.1 5,167,314 - Pf6N2E2_4434 0.85 +0.7 5,167,314 - Pf6N2E2_4434 0.85 -0.5 5,167,314 - Pf6N2E2_4434 0.85 +1.4 5,167,314 - Pf6N2E2_4434 0.85 -0.1 5,167,363 - +1.4 5,167,400 + +1.4 5,167,400 + -1.2 5,167,400 + +0.0 5,167,401 - +1.4 5,167,401 - +1.3 5,167,401 - +0.4 5,167,401 - +1.4 5,167,401 - +3.4 5,167,409 + +0.7 5,167,410 - +2.3 5,167,410 - +1.1 5,167,420 + +0.9 5,167,420 + +0.4 5,167,421 - +2.5 5,167,447 + +0.1 5,167,447 + +0.4 5,167,664 - Pf6N2E2_4435 0.21 +2.4 5,167,681 - Pf6N2E2_4435 0.25 +1.4 5,167,744 + Pf6N2E2_4435 0.39 +1.2 5,167,744 + Pf6N2E2_4435 0.39 +1.4 5,167,744 + Pf6N2E2_4435 0.39 +1.6 5,167,744 + Pf6N2E2_4435 0.39 +1.8 5,167,744 + Pf6N2E2_4435 0.39 +0.5 5,167,744 + Pf6N2E2_4435 0.39 +1.9 5,167,745 - Pf6N2E2_4435 0.39 +0.9 5,167,745 - Pf6N2E2_4435 0.39 +0.4 5,167,745 - Pf6N2E2_4435 0.39 +1.1 5,167,745 - Pf6N2E2_4435 0.39 +1.2 5,167,745 - Pf6N2E2_4435 0.39 +1.4 5,167,745 - Pf6N2E2_4435 0.39 +2.6 5,167,769 + Pf6N2E2_4435 0.44 +0.4 5,167,769 + Pf6N2E2_4435 0.44 +1.4 5,167,769 + Pf6N2E2_4435 0.44 +0.9 5,167,770 - Pf6N2E2_4435 0.44 +1.8 5,167,770 - Pf6N2E2_4435 0.44 +2.7 5,167,771 + Pf6N2E2_4435 0.44 +0.4 5,167,771 + Pf6N2E2_4435 0.44 +0.4 5,167,771 + Pf6N2E2_4435 0.44 +0.4 5,167,771 + Pf6N2E2_4435 0.44 +1.6 5,167,771 + Pf6N2E2_4435 0.44 +2.1 5,167,771 + Pf6N2E2_4435 0.44 +2.4 5,167,771 + Pf6N2E2_4435 0.44 +1.2 5,167,771 + Pf6N2E2_4435 0.44 +0.4 5,167,771 + Pf6N2E2_4435 0.44 +1.7 5,167,772 - Pf6N2E2_4435 0.45 +1.4 5,167,772 - Pf6N2E2_4435 0.45 +0.4 5,167,772 - Pf6N2E2_4435 0.45 +1.7 5,167,772 - Pf6N2E2_4435 0.45 +2.7 5,167,772 - Pf6N2E2_4435 0.45 +0.9 5,167,772 - Pf6N2E2_4435 0.45 +2.5 5,167,772 - Pf6N2E2_4435 0.45 +1.6 5,167,772 - Pf6N2E2_4435 0.45 +0.7 5,167,772 - Pf6N2E2_4435 0.45 +1.9 5,167,772 - Pf6N2E2_4435 0.45 +2.2 5,167,772 - Pf6N2E2_4435 0.45 +1.6 5,167,840 + Pf6N2E2_4435 0.59 +1.5 5,167,841 - Pf6N2E2_4435 0.60 +1.6 5,168,167 + Pf6N2E2_4436 0.12 +0.4 5,168,167 + Pf6N2E2_4436 0.12 +0.9 5,168,319 - Pf6N2E2_4436 0.35 +0.7 5,168,320 + Pf6N2E2_4436 0.35 +0.4 5,168,320 + Pf6N2E2_4436 0.35 +0.9 5,168,320 + Pf6N2E2_4436 0.35 +1.3 5,168,320 + Pf6N2E2_4436 0.35 +0.4 5,168,320 + Pf6N2E2_4436 0.35 -1.0 5,168,320 + Pf6N2E2_4436 0.35 -0.6 5,168,320 + Pf6N2E2_4436 0.35 +0.6 5,168,320 + Pf6N2E2_4436 0.35 +0.7 5,168,320 + Pf6N2E2_4436 0.35 +0.2 5,168,320 + Pf6N2E2_4436 0.35 +0.5 5,168,321 - Pf6N2E2_4436 0.35 -0.1 5,168,321 - Pf6N2E2_4436 0.35 +1.9 5,168,321 - Pf6N2E2_4436 0.35 -0.4 5,168,321 - Pf6N2E2_4436 0.35 +0.4 5,168,321 - Pf6N2E2_4436 0.35 +0.4 5,168,566 + Pf6N2E2_4436 0.72 +0.2 5,168,566 + Pf6N2E2_4436 0.72 -0.0 5,168,567 - Pf6N2E2_4436 0.72 +1.2 5,168,567 - Pf6N2E2_4436 0.72 +0.8 5,168,567 - Pf6N2E2_4436 0.72 +0.7 5,168,567 - Pf6N2E2_4436 0.72 +1.1 5,168,656 + Pf6N2E2_4436 0.86 +2.7 5,168,657 - Pf6N2E2_4436 0.86 +0.5 5,168,657 - Pf6N2E2_4436 0.86 -0.6 5,168,657 - Pf6N2E2_4436 0.86 +1.2 5,168,731 - +1.7 5,168,780 + +0.3 5,168,780 + -1.6 5,168,781 - +0.4 5,168,781 - +0.2 5,168,781 - +0.1 5,168,781 - +1.3 5,168,865 + +0.4 5,168,865 + +1.4 5,168,865 + -1.2 5,168,866 - -0.5 5,168,904 + +0.5 5,168,904 + -0.4 5,168,904 + +0.8 5,168,904 + +0.4 5,168,904 + +0.9 5,168,904 + +0.8 5,168,904 + +0.8 5,168,904 + +0.6 5,168,904 + +0.4 5,168,904 + -0.2 5,168,904 + +0.7 5,168,904 + -0.1 5,168,904 + -0.1 5,168,905 - +1.1 5,168,905 - +0.4 5,168,905 - +0.2 5,168,905 - +1.7 5,168,905 - +0.5 5,168,907 + -0.2 5,168,907 + +1.2 5,168,907 + -1.2 5,168,907 + +0.5 5,168,907 + +0.4 5,168,908 - +1.0 5,168,908 - -0.1 5,168,908 - -0.6 5,168,908 - +0.4 5,168,936 + +1.4 5,168,937 - +1.4 5,168,937 - -0.5 5,168,937 - +0.4 5,168,964 + +1.0 5,168,965 - -0.1 5,168,965 - +1.1
Or see this region's nucleotide sequence