Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4396 and Pf6N2E2_4397 are separated by 56 nucleotides Pf6N2E2_4397 and Pf6N2E2_4398 are separated by 57 nucleotides
Pf6N2E2_4396 - Acetyl-CoA hydrolase, at 5,123,737 to 5,125,659
_4396
Pf6N2E2_4397 - Xanthine phosphoribosyltransferase (EC 2.4.2.22), at 5,125,716 to 5,126,288
_4397
Pf6N2E2_4398 - ATP-dependent DNA helicase Rep, at 5,126,346 to 5,128,355
_4398
Position (kb) 5125
5126
5127 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5124.797 kb on + strand, within Pf6N2E2_4396 at 5124.798 kb on - strand, within Pf6N2E2_4396 at 5124.798 kb on - strand, within Pf6N2E2_4396 at 5124.798 kb on - strand, within Pf6N2E2_4396 at 5124.832 kb on + strand, within Pf6N2E2_4396 at 5124.832 kb on + strand, within Pf6N2E2_4396 at 5124.895 kb on + strand, within Pf6N2E2_4396 at 5124.895 kb on + strand, within Pf6N2E2_4396 at 5124.895 kb on + strand, within Pf6N2E2_4396 at 5124.896 kb on - strand, within Pf6N2E2_4396 at 5124.896 kb on - strand, within Pf6N2E2_4396 at 5124.896 kb on - strand, within Pf6N2E2_4396 at 5124.896 kb on - strand, within Pf6N2E2_4396 at 5124.896 kb on - strand, within Pf6N2E2_4396 at 5124.910 kb on + strand, within Pf6N2E2_4396 at 5124.911 kb on - strand, within Pf6N2E2_4396 at 5124.963 kb on + strand, within Pf6N2E2_4396 at 5124.963 kb on + strand, within Pf6N2E2_4396 at 5124.963 kb on + strand, within Pf6N2E2_4396 at 5124.963 kb on + strand, within Pf6N2E2_4396 at 5124.963 kb on + strand, within Pf6N2E2_4396 at 5125.048 kb on + strand, within Pf6N2E2_4396 at 5125.049 kb on - strand, within Pf6N2E2_4396 at 5125.049 kb on - strand, within Pf6N2E2_4396 at 5125.144 kb on - strand, within Pf6N2E2_4396 at 5125.156 kb on + strand, within Pf6N2E2_4396 at 5125.208 kb on - strand, within Pf6N2E2_4396 at 5125.448 kb on + strand, within Pf6N2E2_4396 at 5125.449 kb on - strand, within Pf6N2E2_4396 at 5125.449 kb on - strand, within Pf6N2E2_4396 at 5125.704 kb on + strand at 5125.704 kb on + strand at 5125.704 kb on + strand at 5125.705 kb on - strand at 5125.705 kb on - strand at 5125.705 kb on - strand at 5125.705 kb on - strand at 5125.705 kb on - strand at 5125.999 kb on + strand, within Pf6N2E2_4397 at 5125.999 kb on + strand, within Pf6N2E2_4397 at 5126.000 kb on - strand, within Pf6N2E2_4397 at 5126.000 kb on - strand, within Pf6N2E2_4397 at 5126.348 kb on - strand at 5126.356 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,124,797 + Pf6N2E2_4396 0.55 +0.2 5,124,798 - Pf6N2E2_4396 0.55 +0.2 5,124,798 - Pf6N2E2_4396 0.55 -1.0 5,124,798 - Pf6N2E2_4396 0.55 -0.3 5,124,832 + Pf6N2E2_4396 0.57 +1.4 5,124,832 + Pf6N2E2_4396 0.57 -0.6 5,124,895 + Pf6N2E2_4396 0.60 +0.4 5,124,895 + Pf6N2E2_4396 0.60 -1.8 5,124,895 + Pf6N2E2_4396 0.60 -0.6 5,124,896 - Pf6N2E2_4396 0.60 +0.0 5,124,896 - Pf6N2E2_4396 0.60 -0.4 5,124,896 - Pf6N2E2_4396 0.60 -0.6 5,124,896 - Pf6N2E2_4396 0.60 +0.4 5,124,896 - Pf6N2E2_4396 0.60 +0.4 5,124,910 + Pf6N2E2_4396 0.61 +0.4 5,124,911 - Pf6N2E2_4396 0.61 +0.6 5,124,963 + Pf6N2E2_4396 0.64 +0.6 5,124,963 + Pf6N2E2_4396 0.64 +0.9 5,124,963 + Pf6N2E2_4396 0.64 +0.4 5,124,963 + Pf6N2E2_4396 0.64 -0.1 5,124,963 + Pf6N2E2_4396 0.64 +0.1 5,125,048 + Pf6N2E2_4396 0.68 +0.7 5,125,049 - Pf6N2E2_4396 0.68 +1.1 5,125,049 - Pf6N2E2_4396 0.68 +0.4 5,125,144 - Pf6N2E2_4396 0.73 -0.8 5,125,156 + Pf6N2E2_4396 0.74 -0.9 5,125,208 - Pf6N2E2_4396 0.76 +0.8 5,125,448 + Pf6N2E2_4396 0.89 +0.0 5,125,449 - Pf6N2E2_4396 0.89 +0.4 5,125,449 - Pf6N2E2_4396 0.89 +0.6 5,125,704 + +0.4 5,125,704 + +2.5 5,125,704 + +0.9 5,125,705 - +1.4 5,125,705 - +1.4 5,125,705 - +0.0 5,125,705 - +1.1 5,125,705 - +0.0 5,125,999 + Pf6N2E2_4397 0.49 -0.4 5,125,999 + Pf6N2E2_4397 0.49 -0.4 5,126,000 - Pf6N2E2_4397 0.50 -0.4 5,126,000 - Pf6N2E2_4397 0.50 -0.6 5,126,348 - +0.4 5,126,356 - +0.2
Or see this region's nucleotide sequence