Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_4350 and Pf6N2E2_4351 are separated by 308 nucleotides Pf6N2E2_4351 and Pf6N2E2_4352 are separated by 148 nucleotides
Pf6N2E2_4350 - RTX toxins and related Ca2+-binding proteins, at 5,078,885 to 5,080,228
_4350
Pf6N2E2_4351 - Predicted signal transduction protein, at 5,080,537 to 5,081,868
_4351
Pf6N2E2_4352 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 5,082,017 to 5,084,092
_4352
Position (kb) 5080
5081
5082 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5079.550 kb on + strand, within Pf6N2E2_4350 at 5079.550 kb on + strand, within Pf6N2E2_4350 at 5079.551 kb on - strand, within Pf6N2E2_4350 at 5079.551 kb on - strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.671 kb on + strand, within Pf6N2E2_4350 at 5079.672 kb on - strand, within Pf6N2E2_4350 at 5079.672 kb on - strand, within Pf6N2E2_4350 at 5079.672 kb on - strand, within Pf6N2E2_4350 at 5079.766 kb on + strand, within Pf6N2E2_4350 at 5079.767 kb on - strand, within Pf6N2E2_4350 at 5079.801 kb on + strand, within Pf6N2E2_4350 at 5079.801 kb on + strand, within Pf6N2E2_4350 at 5079.852 kb on + strand, within Pf6N2E2_4350 at 5079.927 kb on + strand, within Pf6N2E2_4350 at 5079.927 kb on + strand, within Pf6N2E2_4350 at 5079.927 kb on + strand, within Pf6N2E2_4350 at 5079.940 kb on + strand, within Pf6N2E2_4350 at 5079.940 kb on + strand, within Pf6N2E2_4350 at 5079.941 kb on - strand, within Pf6N2E2_4350 at 5079.996 kb on - strand, within Pf6N2E2_4350 at 5080.012 kb on + strand, within Pf6N2E2_4350 at 5080.012 kb on + strand, within Pf6N2E2_4350 at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.103 kb on + strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.104 kb on - strand at 5080.128 kb on + strand at 5080.128 kb on + strand at 5080.128 kb on + strand at 5080.129 kb on - strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.190 kb on + strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.191 kb on - strand at 5080.192 kb on + strand at 5080.192 kb on + strand at 5080.193 kb on - strand at 5080.217 kb on + strand at 5080.226 kb on + strand at 5080.244 kb on + strand at 5080.244 kb on + strand at 5080.244 kb on + strand at 5080.245 kb on - strand at 5080.245 kb on - strand at 5080.245 kb on - strand at 5080.245 kb on - strand at 5080.344 kb on + strand at 5080.344 kb on + strand at 5080.345 kb on - strand at 5080.633 kb on + strand at 5080.634 kb on - strand at 5080.634 kb on - strand at 5080.634 kb on - strand at 5080.706 kb on - strand, within Pf6N2E2_4351 at 5080.740 kb on - strand, within Pf6N2E2_4351 at 5080.868 kb on + strand, within Pf6N2E2_4351 at 5080.868 kb on + strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.869 kb on - strand, within Pf6N2E2_4351 at 5080.962 kb on + strand, within Pf6N2E2_4351 at 5080.963 kb on - strand, within Pf6N2E2_4351 at 5080.982 kb on + strand, within Pf6N2E2_4351 at 5080.982 kb on + strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5080.983 kb on - strand, within Pf6N2E2_4351 at 5081.054 kb on + strand, within Pf6N2E2_4351 at 5081.054 kb on + strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.055 kb on - strand, within Pf6N2E2_4351 at 5081.072 kb on + strand, within Pf6N2E2_4351 at 5081.216 kb on + strand, within Pf6N2E2_4351 at 5081.216 kb on + strand, within Pf6N2E2_4351 at 5081.219 kb on + strand, within Pf6N2E2_4351 at 5081.606 kb on + strand, within Pf6N2E2_4351 at 5081.607 kb on - strand, within Pf6N2E2_4351 at 5081.809 kb on + strand at 5081.809 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.970 kb on + strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.971 kb on - strand at 5081.972 kb on + strand at 5081.973 kb on - strand at 5082.777 kb on + strand, within Pf6N2E2_4352
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 5,079,550 + Pf6N2E2_4350 0.49 +0.0 5,079,550 + Pf6N2E2_4350 0.49 -1.0 5,079,551 - Pf6N2E2_4350 0.50 +0.4 5,079,551 - Pf6N2E2_4350 0.50 +0.5 5,079,671 + Pf6N2E2_4350 0.58 -2.5 5,079,671 + Pf6N2E2_4350 0.58 +0.4 5,079,671 + Pf6N2E2_4350 0.58 -0.0 5,079,671 + Pf6N2E2_4350 0.58 -1.2 5,079,671 + Pf6N2E2_4350 0.58 +1.9 5,079,672 - Pf6N2E2_4350 0.59 +0.4 5,079,672 - Pf6N2E2_4350 0.59 +1.5 5,079,672 - Pf6N2E2_4350 0.59 +0.5 5,079,766 + Pf6N2E2_4350 0.66 +0.8 5,079,767 - Pf6N2E2_4350 0.66 -0.1 5,079,801 + Pf6N2E2_4350 0.68 -0.4 5,079,801 + Pf6N2E2_4350 0.68 -1.0 5,079,852 + Pf6N2E2_4350 0.72 -0.2 5,079,927 + Pf6N2E2_4350 0.78 +0.9 5,079,927 + Pf6N2E2_4350 0.78 +0.4 5,079,927 + Pf6N2E2_4350 0.78 +0.4 5,079,940 + Pf6N2E2_4350 0.78 +0.2 5,079,940 + Pf6N2E2_4350 0.78 -0.8 5,079,941 - Pf6N2E2_4350 0.79 -0.2 5,079,996 - Pf6N2E2_4350 0.83 -1.2 5,080,012 + Pf6N2E2_4350 0.84 -0.2 5,080,012 + Pf6N2E2_4350 0.84 -0.1 5,080,103 + -0.0 5,080,103 + -0.1 5,080,103 + -0.1 5,080,103 + -1.2 5,080,103 + +0.3 5,080,103 + -0.0 5,080,104 - -1.2 5,080,104 - -0.3 5,080,104 - +0.8 5,080,104 - +0.6 5,080,104 - +1.1 5,080,104 - -0.4 5,080,104 - +0.1 5,080,104 - +1.1 5,080,104 - +1.7 5,080,128 + +0.3 5,080,128 + +0.8 5,080,128 + +0.1 5,080,129 - +0.8 5,080,190 + -0.3 5,080,190 + -0.2 5,080,190 + +1.0 5,080,190 + +0.5 5,080,190 + +0.4 5,080,190 + -1.2 5,080,190 + +0.5 5,080,190 + +0.5 5,080,191 - +1.4 5,080,191 - -1.2 5,080,191 - -0.6 5,080,191 - +0.6 5,080,191 - +0.2 5,080,191 - +0.4 5,080,191 - -1.5 5,080,191 - +0.0 5,080,192 + +1.9 5,080,192 + +1.1 5,080,193 - +0.4 5,080,217 + -0.1 5,080,226 + +0.4 5,080,244 + +0.8 5,080,244 + +0.6 5,080,244 + +0.7 5,080,245 - +2.4 5,080,245 - +1.4 5,080,245 - -0.1 5,080,245 - +0.2 5,080,344 + +0.1 5,080,344 + +0.4 5,080,345 - -1.2 5,080,633 + -1.0 5,080,634 - +0.4 5,080,634 - -2.1 5,080,634 - -0.9 5,080,706 - Pf6N2E2_4351 0.13 -1.0 5,080,740 - Pf6N2E2_4351 0.15 +0.6 5,080,868 + Pf6N2E2_4351 0.25 -0.1 5,080,868 + Pf6N2E2_4351 0.25 +0.8 5,080,869 - Pf6N2E2_4351 0.25 +2.4 5,080,869 - Pf6N2E2_4351 0.25 -0.6 5,080,869 - Pf6N2E2_4351 0.25 +0.1 5,080,869 - Pf6N2E2_4351 0.25 -0.4 5,080,869 - Pf6N2E2_4351 0.25 +0.4 5,080,869 - Pf6N2E2_4351 0.25 -1.5 5,080,962 + Pf6N2E2_4351 0.32 -1.5 5,080,963 - Pf6N2E2_4351 0.32 -1.6 5,080,982 + Pf6N2E2_4351 0.33 -0.6 5,080,982 + Pf6N2E2_4351 0.33 -0.0 5,080,983 - Pf6N2E2_4351 0.33 +0.4 5,080,983 - Pf6N2E2_4351 0.33 -1.6 5,080,983 - Pf6N2E2_4351 0.33 -1.0 5,080,983 - Pf6N2E2_4351 0.33 -1.3 5,080,983 - Pf6N2E2_4351 0.33 +0.4 5,081,054 + Pf6N2E2_4351 0.39 -0.8 5,081,054 + Pf6N2E2_4351 0.39 -0.2 5,081,055 - Pf6N2E2_4351 0.39 -0.5 5,081,055 - Pf6N2E2_4351 0.39 -1.1 5,081,055 - Pf6N2E2_4351 0.39 -1.0 5,081,055 - Pf6N2E2_4351 0.39 -1.5 5,081,055 - Pf6N2E2_4351 0.39 +0.5 5,081,055 - Pf6N2E2_4351 0.39 -1.6 5,081,055 - Pf6N2E2_4351 0.39 +0.5 5,081,055 - Pf6N2E2_4351 0.39 +0.8 5,081,072 + Pf6N2E2_4351 0.40 -0.4 5,081,216 + Pf6N2E2_4351 0.51 -0.5 5,081,216 + Pf6N2E2_4351 0.51 -0.6 5,081,219 + Pf6N2E2_4351 0.51 -1.6 5,081,606 + Pf6N2E2_4351 0.80 -0.3 5,081,607 - Pf6N2E2_4351 0.80 +0.7 5,081,809 + -0.1 5,081,809 + -0.4 5,081,970 + +0.4 5,081,970 + -0.6 5,081,970 + -0.6 5,081,970 + -0.3 5,081,970 + +0.1 5,081,970 + -0.6 5,081,971 - +1.4 5,081,971 - +0.4 5,081,971 - +0.5 5,081,971 - +0.7 5,081,972 + +1.4 5,081,973 - -1.0 5,082,777 + Pf6N2E2_4352 0.37 +0.4
Or see this region's nucleotide sequence