Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_408 and Pf6N2E2_409 are separated by 110 nucleotides Pf6N2E2_409 and Pf6N2E2_410 are separated by 219 nucleotides Pf6N2E2_410 and Pf6N2E2_411 are separated by 76 nucleotides
Pf6N2E2_408 - Transcriptional regulators, LysR family, at 477,998 to 478,864
_408
Pf6N2E2_409 - Permease of the drug/metabolite transporter (DMT) superfamily, at 478,975 to 479,898
_409
Pf6N2E2_410 - hypothetical protein, at 480,118 to 480,660
_410
Pf6N2E2_411 - Nitrogen regulation protein NR(I), at 480,737 to 480,892
_411
Position (kb) 478
479
480 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 477.995 kb on + strand at 477.995 kb on + strand at 477.995 kb on + strand at 477.996 kb on - strand at 477.996 kb on - strand at 477.996 kb on - strand at 477.996 kb on - strand at 477.996 kb on - strand at 477.999 kb on + strand at 477.999 kb on + strand at 478.000 kb on - strand at 478.029 kb on - strand at 478.074 kb on + strand at 478.074 kb on + strand at 478.074 kb on + strand at 478.113 kb on + strand, within Pf6N2E2_408 at 478.114 kb on - strand, within Pf6N2E2_408 at 478.149 kb on + strand, within Pf6N2E2_408 at 478.149 kb on + strand, within Pf6N2E2_408 at 478.150 kb on - strand, within Pf6N2E2_408 at 478.150 kb on - strand, within Pf6N2E2_408 at 478.213 kb on - strand, within Pf6N2E2_408 at 478.315 kb on + strand, within Pf6N2E2_408 at 478.316 kb on - strand, within Pf6N2E2_408 at 478.391 kb on + strand, within Pf6N2E2_408 at 478.392 kb on - strand, within Pf6N2E2_408 at 478.519 kb on - strand, within Pf6N2E2_408 at 478.530 kb on + strand, within Pf6N2E2_408 at 478.530 kb on + strand, within Pf6N2E2_408 at 478.530 kb on + strand, within Pf6N2E2_408 at 478.541 kb on + strand, within Pf6N2E2_408 at 478.541 kb on + strand, within Pf6N2E2_408 at 478.584 kb on - strand, within Pf6N2E2_408 at 478.897 kb on - strand at 478.907 kb on + strand at 478.907 kb on + strand at 478.908 kb on - strand at 478.929 kb on + strand at 478.930 kb on - strand at 478.939 kb on + strand at 479.030 kb on + strand at 479.074 kb on + strand, within Pf6N2E2_409 at 479.074 kb on + strand, within Pf6N2E2_409 at 479.075 kb on - strand, within Pf6N2E2_409 at 479.110 kb on + strand, within Pf6N2E2_409 at 479.110 kb on + strand, within Pf6N2E2_409 at 479.111 kb on - strand, within Pf6N2E2_409 at 479.186 kb on + strand, within Pf6N2E2_409 at 479.213 kb on + strand, within Pf6N2E2_409 at 479.272 kb on + strand, within Pf6N2E2_409 at 479.506 kb on + strand, within Pf6N2E2_409 at 479.507 kb on - strand, within Pf6N2E2_409 at 479.530 kb on - strand, within Pf6N2E2_409 at 479.539 kb on + strand, within Pf6N2E2_409 at 479.563 kb on + strand, within Pf6N2E2_409 at 479.563 kb on + strand, within Pf6N2E2_409 at 479.693 kb on - strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.707 kb on + strand, within Pf6N2E2_409 at 479.708 kb on - strand, within Pf6N2E2_409 at 479.708 kb on - strand, within Pf6N2E2_409 at 479.708 kb on - strand, within Pf6N2E2_409 at 479.752 kb on + strand, within Pf6N2E2_409 at 479.913 kb on + strand at 480.120 kb on - strand at 480.135 kb on - strand at 480.719 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 477,995 + +1.1 477,995 + +0.3 477,995 + -0.3 477,996 - +0.3 477,996 - +0.2 477,996 - +0.4 477,996 - +1.4 477,996 - -0.4 477,999 + -0.1 477,999 + -0.6 478,000 - -0.1 478,029 - -1.3 478,074 + +1.3 478,074 + +2.0 478,074 + -0.3 478,113 + Pf6N2E2_408 0.13 -0.1 478,114 - Pf6N2E2_408 0.13 +0.7 478,149 + Pf6N2E2_408 0.17 -0.6 478,149 + Pf6N2E2_408 0.17 +0.4 478,150 - Pf6N2E2_408 0.18 -0.1 478,150 - Pf6N2E2_408 0.18 +0.2 478,213 - Pf6N2E2_408 0.25 +1.2 478,315 + Pf6N2E2_408 0.37 +0.7 478,316 - Pf6N2E2_408 0.37 +0.5 478,391 + Pf6N2E2_408 0.45 -2.9 478,392 - Pf6N2E2_408 0.45 -0.3 478,519 - Pf6N2E2_408 0.60 +1.4 478,530 + Pf6N2E2_408 0.61 +0.2 478,530 + Pf6N2E2_408 0.61 +0.7 478,530 + Pf6N2E2_408 0.61 +1.8 478,541 + Pf6N2E2_408 0.63 -0.3 478,541 + Pf6N2E2_408 0.63 +1.0 478,584 - Pf6N2E2_408 0.68 -2.3 478,897 - +1.1 478,907 + +1.0 478,907 + -0.3 478,908 - -0.3 478,929 + -0.2 478,930 - +2.7 478,939 + +2.5 479,030 + +1.3 479,074 + Pf6N2E2_409 0.11 +0.7 479,074 + Pf6N2E2_409 0.11 +0.6 479,075 - Pf6N2E2_409 0.11 -1.3 479,110 + Pf6N2E2_409 0.15 +0.0 479,110 + Pf6N2E2_409 0.15 +0.0 479,111 - Pf6N2E2_409 0.15 +1.5 479,186 + Pf6N2E2_409 0.23 +0.2 479,213 + Pf6N2E2_409 0.26 +1.0 479,272 + Pf6N2E2_409 0.32 +0.3 479,506 + Pf6N2E2_409 0.57 +1.3 479,507 - Pf6N2E2_409 0.58 -0.3 479,530 - Pf6N2E2_409 0.60 -0.3 479,539 + Pf6N2E2_409 0.61 +1.5 479,563 + Pf6N2E2_409 0.64 -0.2 479,563 + Pf6N2E2_409 0.64 +0.4 479,693 - Pf6N2E2_409 0.78 -0.9 479,707 + Pf6N2E2_409 0.79 +0.6 479,707 + Pf6N2E2_409 0.79 +0.5 479,707 + Pf6N2E2_409 0.79 +0.2 479,707 + Pf6N2E2_409 0.79 -0.0 479,707 + Pf6N2E2_409 0.79 +1.0 479,708 - Pf6N2E2_409 0.79 -0.6 479,708 - Pf6N2E2_409 0.79 -1.3 479,708 - Pf6N2E2_409 0.79 +0.7 479,752 + Pf6N2E2_409 0.84 +0.4 479,913 + -0.5 480,120 - -0.3 480,135 - +0.1 480,719 - -0.3
Or see this region's nucleotide sequence