Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_3822

Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_3821 and Pf6N2E2_3822 are separated by 121 nucleotidesPf6N2E2_3822 and Pf6N2E2_3823 are separated by 103 nucleotidesPf6N2E2_3823 and Pf6N2E2_3824 overlap by 4 nucleotides Pf6N2E2_3821 - Glutamine synthetase type I (EC 6.3.1.2), at 4,456,276 to 4,457,682 _3821 Pf6N2E2_3822 - Periplasmic chorismate mutase I precursor (EC 5.4.99.5), at 4,457,804 to 4,458,367 _3822 Pf6N2E2_3823 - FIG00956533: hypothetical protein, at 4,458,471 to 4,458,989 _3823 Pf6N2E2_3824 - FIG00953806: hypothetical protein, at 4,458,986 to 4,459,609 _3824 Position (kb) 4457 4458 4459Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4457.688 kb on + strandat 4457.689 kb on - strandat 4457.758 kb on - strandat 4457.786 kb on + strandat 4457.786 kb on + strandat 4457.786 kb on + strandat 4457.786 kb on + strandat 4457.786 kb on + strandat 4457.787 kb on - strandat 4457.787 kb on - strandat 4457.787 kb on - strandat 4457.787 kb on - strandat 4457.788 kb on + strandat 4457.788 kb on + strandat 4457.788 kb on + strandat 4457.788 kb on + strandat 4457.788 kb on + strandat 4457.788 kb on + strandat 4457.788 kb on + strandat 4457.788 kb on + strandat 4457.788 kb on + strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.789 kb on - strandat 4457.971 kb on + strand, within Pf6N2E2_3822at 4457.971 kb on + strand, within Pf6N2E2_3822at 4457.971 kb on + strand, within Pf6N2E2_3822at 4457.971 kb on + strand, within Pf6N2E2_3822at 4457.972 kb on - strand, within Pf6N2E2_3822at 4458.038 kb on + strand, within Pf6N2E2_3822at 4458.039 kb on - strand, within Pf6N2E2_3822at 4458.039 kb on - strand, within Pf6N2E2_3822at 4458.084 kb on + strand, within Pf6N2E2_3822at 4458.084 kb on + strand, within Pf6N2E2_3822at 4458.084 kb on + strand, within Pf6N2E2_3822at 4458.085 kb on - strand, within Pf6N2E2_3822at 4458.085 kb on - strand, within Pf6N2E2_3822at 4458.085 kb on - strand, within Pf6N2E2_3822at 4458.107 kb on + strand, within Pf6N2E2_3822at 4458.107 kb on + strand, within Pf6N2E2_3822at 4458.107 kb on + strand, within Pf6N2E2_3822at 4458.107 kb on + strand, within Pf6N2E2_3822at 4458.107 kb on + strand, within Pf6N2E2_3822at 4458.108 kb on - strand, within Pf6N2E2_3822at 4458.108 kb on - strand, within Pf6N2E2_3822at 4458.108 kb on - strand, within Pf6N2E2_3822at 4458.108 kb on - strand, within Pf6N2E2_3822at 4458.108 kb on - strand, within Pf6N2E2_3822at 4458.108 kb on - strand, within Pf6N2E2_3822at 4458.108 kb on - strand, within Pf6N2E2_3822at 4458.395 kb on + strandat 4458.395 kb on + strandat 4458.395 kb on + strandat 4458.396 kb on - strandat 4458.396 kb on - strandat 4458.432 kb on + strandat 4458.432 kb on + strandat 4458.432 kb on + strandat 4458.432 kb on + strandat 4458.432 kb on + strandat 4458.432 kb on + strandat 4458.433 kb on - strandat 4458.433 kb on - strandat 4458.433 kb on - strandat 4458.433 kb on - strandat 4458.433 kb on - strandat 4458.534 kb on + strand, within Pf6N2E2_3823at 4458.534 kb on + strand, within Pf6N2E2_3823at 4458.534 kb on + strand, within Pf6N2E2_3823at 4458.534 kb on + strand, within Pf6N2E2_3823at 4458.534 kb on + strand, within Pf6N2E2_3823at 4458.534 kb on + strand, within Pf6N2E2_3823at 4458.535 kb on - strand, within Pf6N2E2_3823at 4458.535 kb on - strand, within Pf6N2E2_3823at 4458.540 kb on + strand, within Pf6N2E2_3823at 4458.540 kb on + strand, within Pf6N2E2_3823at 4458.541 kb on - strand, within Pf6N2E2_3823at 4458.541 kb on - strand, within Pf6N2E2_3823at 4458.541 kb on - strand, within Pf6N2E2_3823at 4458.541 kb on - strand, within Pf6N2E2_3823at 4458.567 kb on + strand, within Pf6N2E2_3823at 4458.567 kb on + strand, within Pf6N2E2_3823at 4458.567 kb on + strand, within Pf6N2E2_3823at 4458.567 kb on + strand, within Pf6N2E2_3823at 4458.568 kb on - strand, within Pf6N2E2_3823at 4458.702 kb on + strand, within Pf6N2E2_3823at 4458.703 kb on - strand, within Pf6N2E2_3823at 4458.703 kb on - strand, within Pf6N2E2_3823at 4458.709 kb on + strand, within Pf6N2E2_3823at 4458.710 kb on - strand, within Pf6N2E2_3823at 4458.840 kb on + strand, within Pf6N2E2_3823at 4458.841 kb on - strand, within Pf6N2E2_3823at 4459.049 kb on + strand, within Pf6N2E2_3824at 4459.049 kb on + strand, within Pf6N2E2_3824at 4459.049 kb on + strand, within Pf6N2E2_3824at 4459.049 kb on + strand, within Pf6N2E2_3824at 4459.049 kb on + strand, within Pf6N2E2_3824at 4459.049 kb on + strand, within Pf6N2E2_3824at 4459.049 kb on + strand, within Pf6N2E2_3824at 4459.049 kb on + strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.050 kb on - strand, within Pf6N2E2_3824at 4459.051 kb on + strand, within Pf6N2E2_3824at 4459.051 kb on + strand, within Pf6N2E2_3824at 4459.051 kb on + strand, within Pf6N2E2_3824at 4459.051 kb on + strand, within Pf6N2E2_3824at 4459.051 kb on + strand, within Pf6N2E2_3824at 4459.052 kb on - strand, within Pf6N2E2_3824at 4459.052 kb on - strand, within Pf6N2E2_3824at 4459.052 kb on - strand, within Pf6N2E2_3824at 4459.052 kb on - strand, within Pf6N2E2_3824at 4459.052 kb on - strand, within Pf6N2E2_3824at 4459.055 kb on + strand, within Pf6N2E2_3824at 4459.055 kb on + strand, within Pf6N2E2_3824at 4459.055 kb on + strand, within Pf6N2E2_3824at 4459.055 kb on + strand, within Pf6N2E2_3824at 4459.055 kb on + strand, within Pf6N2E2_3824at 4459.055 kb on + strand, within Pf6N2E2_3824at 4459.055 kb on + strand, within Pf6N2E2_3824at 4459.055 kb on + strand, within Pf6N2E2_3824at 4459.056 kb on - strand, within Pf6N2E2_3824at 4459.056 kb on - strand, within Pf6N2E2_3824at 4459.056 kb on - strand, within Pf6N2E2_3824at 4459.056 kb on - strand, within Pf6N2E2_3824at 4459.119 kb on + strand, within Pf6N2E2_3824at 4459.119 kb on + strand, within Pf6N2E2_3824at 4459.119 kb on + strand, within Pf6N2E2_3824at 4459.120 kb on - strand, within Pf6N2E2_3824at 4459.120 kb on - strand, within Pf6N2E2_3824at 4459.120 kb on - strand, within Pf6N2E2_3824at 4459.120 kb on - strand, within Pf6N2E2_3824

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Per-strain Table

Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
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4,457,688 + -0.0
4,457,689 - -1.8
4,457,758 - +0.5
4,457,786 + +0.1
4,457,786 + +1.0
4,457,786 + +1.4
4,457,786 + -0.2
4,457,786 + -0.0
4,457,787 - -0.6
4,457,787 - -0.0
4,457,787 - +0.5
4,457,787 - +0.2
4,457,788 + +0.4
4,457,788 + +0.4
4,457,788 + +0.3
4,457,788 + +0.6
4,457,788 + +0.0
4,457,788 + -0.1
4,457,788 + -1.0
4,457,788 + +1.4
4,457,788 + +1.5
4,457,789 - +0.5
4,457,789 - +1.7
4,457,789 - +0.3
4,457,789 - +0.4
4,457,789 - -0.0
4,457,789 - +0.6
4,457,789 - +0.4
4,457,789 - +0.1
4,457,789 - +2.0
4,457,789 - -0.9
4,457,789 - +0.4
4,457,789 - +0.2
4,457,971 + Pf6N2E2_3822 0.30 +0.1
4,457,971 + Pf6N2E2_3822 0.30 -0.1
4,457,971 + Pf6N2E2_3822 0.30 +0.4
4,457,971 + Pf6N2E2_3822 0.30 +0.4
4,457,972 - Pf6N2E2_3822 0.30 -0.9
4,458,038 + Pf6N2E2_3822 0.41 +0.1
4,458,039 - Pf6N2E2_3822 0.42 +0.1
4,458,039 - Pf6N2E2_3822 0.42 -2.0
4,458,084 + Pf6N2E2_3822 0.50 +0.4
4,458,084 + Pf6N2E2_3822 0.50 +1.4
4,458,084 + Pf6N2E2_3822 0.50 +0.2
4,458,085 - Pf6N2E2_3822 0.50 -0.1
4,458,085 - Pf6N2E2_3822 0.50 +1.1
4,458,085 - Pf6N2E2_3822 0.50 -0.4
4,458,107 + Pf6N2E2_3822 0.54 +0.1
4,458,107 + Pf6N2E2_3822 0.54 +2.0
4,458,107 + Pf6N2E2_3822 0.54 -1.6
4,458,107 + Pf6N2E2_3822 0.54 +2.0
4,458,107 + Pf6N2E2_3822 0.54 +1.0
4,458,108 - Pf6N2E2_3822 0.54 -0.2
4,458,108 - Pf6N2E2_3822 0.54 -0.5
4,458,108 - Pf6N2E2_3822 0.54 +0.4
4,458,108 - Pf6N2E2_3822 0.54 +0.1
4,458,108 - Pf6N2E2_3822 0.54 -0.4
4,458,108 - Pf6N2E2_3822 0.54 -0.1
4,458,108 - Pf6N2E2_3822 0.54 -0.0
4,458,395 + +0.2
4,458,395 + +0.4
4,458,395 + +1.0
4,458,396 - +1.7
4,458,396 - -0.9
4,458,432 + -0.6
4,458,432 + -0.2
4,458,432 + +1.1
4,458,432 + +1.2
4,458,432 + +0.1
4,458,432 + +1.4
4,458,433 - -0.0
4,458,433 - +0.2
4,458,433 - +0.1
4,458,433 - +1.0
4,458,433 - +0.2
4,458,534 + Pf6N2E2_3823 0.12 +0.4
4,458,534 + Pf6N2E2_3823 0.12 -0.2
4,458,534 + Pf6N2E2_3823 0.12 +0.5
4,458,534 + Pf6N2E2_3823 0.12 +0.3
4,458,534 + Pf6N2E2_3823 0.12 +0.4
4,458,534 + Pf6N2E2_3823 0.12 +0.9
4,458,535 - Pf6N2E2_3823 0.12 -1.2
4,458,535 - Pf6N2E2_3823 0.12 -1.0
4,458,540 + Pf6N2E2_3823 0.13 +1.3
4,458,540 + Pf6N2E2_3823 0.13 +0.4
4,458,541 - Pf6N2E2_3823 0.13 -0.6
4,458,541 - Pf6N2E2_3823 0.13 +0.8
4,458,541 - Pf6N2E2_3823 0.13 +0.1
4,458,541 - Pf6N2E2_3823 0.13 +0.4
4,458,567 + Pf6N2E2_3823 0.18 +1.2
4,458,567 + Pf6N2E2_3823 0.18 -0.0
4,458,567 + Pf6N2E2_3823 0.18 -1.0
4,458,567 + Pf6N2E2_3823 0.18 +0.1
4,458,568 - Pf6N2E2_3823 0.19 -0.1
4,458,702 + Pf6N2E2_3823 0.45 +0.5
4,458,703 - Pf6N2E2_3823 0.45 +0.5
4,458,703 - Pf6N2E2_3823 0.45 -0.9
4,458,709 + Pf6N2E2_3823 0.46 +1.0
4,458,710 - Pf6N2E2_3823 0.46 +1.0
4,458,840 + Pf6N2E2_3823 0.71 +0.4
4,458,841 - Pf6N2E2_3823 0.71 +0.7
4,459,049 + Pf6N2E2_3824 0.10 +1.0
4,459,049 + Pf6N2E2_3824 0.10 +0.2
4,459,049 + Pf6N2E2_3824 0.10 +0.8
4,459,049 + Pf6N2E2_3824 0.10 +3.0
4,459,049 + Pf6N2E2_3824 0.10 +0.4
4,459,049 + Pf6N2E2_3824 0.10 -0.4
4,459,049 + Pf6N2E2_3824 0.10 -0.0
4,459,049 + Pf6N2E2_3824 0.10 +0.1
4,459,050 - Pf6N2E2_3824 0.10 -1.1
4,459,050 - Pf6N2E2_3824 0.10 +1.0
4,459,050 - Pf6N2E2_3824 0.10 -0.1
4,459,050 - Pf6N2E2_3824 0.10 +2.0
4,459,050 - Pf6N2E2_3824 0.10 +1.1
4,459,050 - Pf6N2E2_3824 0.10 +0.4
4,459,050 - Pf6N2E2_3824 0.10 -0.2
4,459,050 - Pf6N2E2_3824 0.10 +0.6
4,459,050 - Pf6N2E2_3824 0.10 +0.4
4,459,050 - Pf6N2E2_3824 0.10 +0.8
4,459,050 - Pf6N2E2_3824 0.10 -0.7
4,459,050 - Pf6N2E2_3824 0.10 -0.5
4,459,051 + Pf6N2E2_3824 0.10 +1.0
4,459,051 + Pf6N2E2_3824 0.10 -1.0
4,459,051 + Pf6N2E2_3824 0.10 -0.3
4,459,051 + Pf6N2E2_3824 0.10 +0.7
4,459,051 + Pf6N2E2_3824 0.10 +0.2
4,459,052 - Pf6N2E2_3824 0.11 +0.3
4,459,052 - Pf6N2E2_3824 0.11 +1.4
4,459,052 - Pf6N2E2_3824 0.11 -1.1
4,459,052 - Pf6N2E2_3824 0.11 -0.4
4,459,052 - Pf6N2E2_3824 0.11 +1.4
4,459,055 + Pf6N2E2_3824 0.11 +0.1
4,459,055 + Pf6N2E2_3824 0.11 +0.5
4,459,055 + Pf6N2E2_3824 0.11 +0.1
4,459,055 + Pf6N2E2_3824 0.11 +0.4
4,459,055 + Pf6N2E2_3824 0.11 -0.3
4,459,055 + Pf6N2E2_3824 0.11 +1.4
4,459,055 + Pf6N2E2_3824 0.11 +0.6
4,459,055 + Pf6N2E2_3824 0.11 +0.4
4,459,056 - Pf6N2E2_3824 0.11 -0.1
4,459,056 - Pf6N2E2_3824 0.11 +0.8
4,459,056 - Pf6N2E2_3824 0.11 -0.1
4,459,056 - Pf6N2E2_3824 0.11 -0.4
4,459,119 + Pf6N2E2_3824 0.21 -0.2
4,459,119 + Pf6N2E2_3824 0.21 +1.3
4,459,119 + Pf6N2E2_3824 0.21 +1.7
4,459,120 - Pf6N2E2_3824 0.21 +0.6
4,459,120 - Pf6N2E2_3824 0.21 +1.7
4,459,120 - Pf6N2E2_3824 0.21 -0.3
4,459,120 - Pf6N2E2_3824 0.21 +0.6

Or see this region's nucleotide sequence