Experiment: 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_3733 and Pf6N2E2_3734 are separated by 254 nucleotides Pf6N2E2_3734 and Pf6N2E2_3735 are separated by 368 nucleotides
Pf6N2E2_3733 - hypothetical protein, at 4,346,017 to 4,350,081
_3733
Pf6N2E2_3734 - Queuosine Biosynthesis QueC ATPase, at 4,350,336 to 4,351,040
_3734
Pf6N2E2_3735 - LysR family transcriptional regulator YnfL, at 4,351,409 to 4,352,314
_3735
Position (kb) 4350
4351
4352 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4349.366 kb on + strand, within Pf6N2E2_3733 at 4349.366 kb on + strand, within Pf6N2E2_3733 at 4349.366 kb on + strand, within Pf6N2E2_3733 at 4349.367 kb on - strand, within Pf6N2E2_3733 at 4349.377 kb on - strand, within Pf6N2E2_3733 at 4349.456 kb on + strand, within Pf6N2E2_3733 at 4349.457 kb on - strand, within Pf6N2E2_3733 at 4349.457 kb on - strand, within Pf6N2E2_3733 at 4349.457 kb on - strand, within Pf6N2E2_3733 at 4349.457 kb on - strand, within Pf6N2E2_3733 at 4349.476 kb on + strand, within Pf6N2E2_3733 at 4349.476 kb on + strand, within Pf6N2E2_3733 at 4349.477 kb on - strand, within Pf6N2E2_3733 at 4349.477 kb on - strand, within Pf6N2E2_3733 at 4349.478 kb on + strand, within Pf6N2E2_3733 at 4349.478 kb on + strand, within Pf6N2E2_3733 at 4349.478 kb on + strand, within Pf6N2E2_3733 at 4349.478 kb on + strand, within Pf6N2E2_3733 at 4349.479 kb on - strand, within Pf6N2E2_3733 at 4349.479 kb on - strand, within Pf6N2E2_3733 at 4349.479 kb on - strand, within Pf6N2E2_3733 at 4349.479 kb on - strand, within Pf6N2E2_3733 at 4349.479 kb on - strand, within Pf6N2E2_3733 at 4349.507 kb on - strand, within Pf6N2E2_3733 at 4349.599 kb on + strand, within Pf6N2E2_3733 at 4349.600 kb on - strand, within Pf6N2E2_3733 at 4349.600 kb on - strand, within Pf6N2E2_3733 at 4349.661 kb on + strand, within Pf6N2E2_3733 at 4349.690 kb on - strand at 4349.754 kb on + strand at 4349.775 kb on - strand at 4349.798 kb on + strand at 4349.849 kb on - strand at 4349.941 kb on + strand at 4349.942 kb on - strand at 4349.942 kb on - strand at 4349.942 kb on - strand at 4349.942 kb on - strand at 4349.942 kb on - strand at 4349.944 kb on + strand at 4349.944 kb on + strand at 4349.945 kb on - strand at 4349.945 kb on - strand at 4349.951 kb on - strand at 4350.030 kb on - strand at 4350.030 kb on - strand at 4350.043 kb on + strand at 4350.049 kb on + strand at 4350.049 kb on + strand at 4350.060 kb on + strand at 4350.079 kb on + strand at 4350.257 kb on - strand at 4350.257 kb on - strand at 4350.320 kb on - strand at 4350.320 kb on - strand at 4350.320 kb on - strand at 4350.320 kb on - strand at 4350.521 kb on + strand, within Pf6N2E2_3734 at 4350.522 kb on - strand, within Pf6N2E2_3734 at 4350.522 kb on - strand, within Pf6N2E2_3734 at 4350.522 kb on - strand, within Pf6N2E2_3734 at 4350.522 kb on - strand, within Pf6N2E2_3734 at 4350.526 kb on + strand, within Pf6N2E2_3734 at 4350.671 kb on + strand, within Pf6N2E2_3734 at 4350.672 kb on - strand, within Pf6N2E2_3734 at 4350.672 kb on - strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.675 kb on + strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.676 kb on - strand, within Pf6N2E2_3734 at 4350.682 kb on - strand, within Pf6N2E2_3734 at 4350.704 kb on + strand, within Pf6N2E2_3734 at 4350.704 kb on + strand, within Pf6N2E2_3734 at 4350.705 kb on - strand, within Pf6N2E2_3734 at 4350.802 kb on - strand, within Pf6N2E2_3734 at 4350.912 kb on + strand, within Pf6N2E2_3734 at 4350.912 kb on + strand, within Pf6N2E2_3734 at 4350.912 kb on + strand, within Pf6N2E2_3734 at 4350.912 kb on + strand, within Pf6N2E2_3734 at 4350.912 kb on + strand, within Pf6N2E2_3734 at 4350.912 kb on + strand, within Pf6N2E2_3734 at 4350.912 kb on + strand, within Pf6N2E2_3734 at 4350.912 kb on + strand, within Pf6N2E2_3734 at 4350.913 kb on - strand, within Pf6N2E2_3734 at 4350.913 kb on - strand, within Pf6N2E2_3734 at 4350.913 kb on - strand, within Pf6N2E2_3734 at 4350.913 kb on - strand, within Pf6N2E2_3734 at 4350.913 kb on - strand, within Pf6N2E2_3734 at 4350.913 kb on - strand, within Pf6N2E2_3734 at 4350.984 kb on + strand at 4350.985 kb on - strand at 4351.036 kb on + strand at 4351.036 kb on + strand at 4351.038 kb on + strand at 4351.039 kb on - strand at 4351.039 kb on - strand at 4351.066 kb on + strand at 4351.076 kb on + strand at 4351.092 kb on + strand at 4351.092 kb on + strand at 4351.092 kb on + strand at 4351.092 kb on + strand at 4351.093 kb on - strand at 4351.093 kb on - strand at 4351.093 kb on - strand at 4351.093 kb on - strand at 4351.093 kb on - strand at 4351.093 kb on - strand at 4351.113 kb on - strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.116 kb on + strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.117 kb on - strand at 4351.154 kb on + strand at 4351.159 kb on + strand at 4351.159 kb on + strand at 4351.159 kb on + strand at 4351.159 kb on + strand at 4351.160 kb on - strand at 4351.160 kb on - strand at 4351.388 kb on + strand at 4351.388 kb on + strand at 4351.388 kb on + strand at 4351.388 kb on + strand at 4351.388 kb on + strand at 4351.388 kb on + strand at 4351.388 kb on + strand at 4351.388 kb on + strand at 4351.389 kb on - strand at 4351.389 kb on - strand at 4351.389 kb on - strand at 4351.389 kb on - strand at 4351.389 kb on - strand at 4351.390 kb on + strand at 4351.390 kb on + strand at 4351.390 kb on + strand at 4351.391 kb on - strand at 4351.391 kb on - strand at 4351.391 kb on - strand at 4351.391 kb on - strand at 4351.391 kb on - strand at 4351.391 kb on - strand at 4351.391 kb on - strand at 4351.404 kb on - strand at 4351.431 kb on - strand at 4351.431 kb on - strand at 4351.432 kb on + strand at 4351.569 kb on + strand, within Pf6N2E2_3735 at 4351.570 kb on - strand, within Pf6N2E2_3735 at 4351.570 kb on - strand, within Pf6N2E2_3735 at 4351.714 kb on - strand, within Pf6N2E2_3735 at 4351.892 kb on + strand, within Pf6N2E2_3735
Per-strain Table
Position Strand Gene LocusTag Fraction 50% P. fluorescens FW300-N2E2 spent media from growth in 20 mM putrescine remove 4,349,366 + Pf6N2E2_3733 0.82 +0.9 4,349,366 + Pf6N2E2_3733 0.82 +2.1 4,349,366 + Pf6N2E2_3733 0.82 +0.1 4,349,367 - Pf6N2E2_3733 0.82 +1.4 4,349,377 - Pf6N2E2_3733 0.83 -0.6 4,349,456 + Pf6N2E2_3733 0.85 +1.8 4,349,457 - Pf6N2E2_3733 0.85 +0.7 4,349,457 - Pf6N2E2_3733 0.85 +0.1 4,349,457 - Pf6N2E2_3733 0.85 -0.2 4,349,457 - Pf6N2E2_3733 0.85 +0.4 4,349,476 + Pf6N2E2_3733 0.85 +1.1 4,349,476 + Pf6N2E2_3733 0.85 +0.7 4,349,477 - Pf6N2E2_3733 0.85 -0.2 4,349,477 - Pf6N2E2_3733 0.85 +0.5 4,349,478 + Pf6N2E2_3733 0.85 +0.3 4,349,478 + Pf6N2E2_3733 0.85 +0.4 4,349,478 + Pf6N2E2_3733 0.85 +1.1 4,349,478 + Pf6N2E2_3733 0.85 -0.2 4,349,479 - Pf6N2E2_3733 0.85 -0.9 4,349,479 - Pf6N2E2_3733 0.85 -0.3 4,349,479 - Pf6N2E2_3733 0.85 +0.5 4,349,479 - Pf6N2E2_3733 0.85 +0.1 4,349,479 - Pf6N2E2_3733 0.85 +0.0 4,349,507 - Pf6N2E2_3733 0.86 +0.6 4,349,599 + Pf6N2E2_3733 0.88 +0.4 4,349,600 - Pf6N2E2_3733 0.88 +2.0 4,349,600 - Pf6N2E2_3733 0.88 +0.7 4,349,661 + Pf6N2E2_3733 0.90 -0.5 4,349,690 - +0.5 4,349,754 + +1.0 4,349,775 - +0.4 4,349,798 + +0.2 4,349,849 - +0.4 4,349,941 + -0.4 4,349,942 - +0.8 4,349,942 - +1.0 4,349,942 - -0.6 4,349,942 - +0.4 4,349,942 - +1.4 4,349,944 + +1.7 4,349,944 + +0.2 4,349,945 - +0.5 4,349,945 - +1.4 4,349,951 - +1.4 4,350,030 - -1.2 4,350,030 - +0.4 4,350,043 + +0.4 4,350,049 + -0.3 4,350,049 + -0.3 4,350,060 + +0.1 4,350,079 + +0.1 4,350,257 - +0.6 4,350,257 - +1.4 4,350,320 - +2.0 4,350,320 - +0.0 4,350,320 - -0.0 4,350,320 - -0.0 4,350,521 + Pf6N2E2_3734 0.26 +1.5 4,350,522 - Pf6N2E2_3734 0.26 +1.1 4,350,522 - Pf6N2E2_3734 0.26 -0.7 4,350,522 - Pf6N2E2_3734 0.26 -0.3 4,350,522 - Pf6N2E2_3734 0.26 +0.3 4,350,526 + Pf6N2E2_3734 0.27 +0.4 4,350,671 + Pf6N2E2_3734 0.48 +0.1 4,350,672 - Pf6N2E2_3734 0.48 +1.1 4,350,672 - Pf6N2E2_3734 0.48 +0.6 4,350,675 + Pf6N2E2_3734 0.48 -0.3 4,350,675 + Pf6N2E2_3734 0.48 +0.4 4,350,675 + Pf6N2E2_3734 0.48 +1.4 4,350,675 + Pf6N2E2_3734 0.48 +1.1 4,350,675 + Pf6N2E2_3734 0.48 -0.5 4,350,675 + Pf6N2E2_3734 0.48 +0.7 4,350,675 + Pf6N2E2_3734 0.48 -0.3 4,350,675 + Pf6N2E2_3734 0.48 +0.4 4,350,675 + Pf6N2E2_3734 0.48 +0.6 4,350,675 + Pf6N2E2_3734 0.48 +0.4 4,350,675 + Pf6N2E2_3734 0.48 +1.0 4,350,675 + Pf6N2E2_3734 0.48 -0.2 4,350,675 + Pf6N2E2_3734 0.48 -1.9 4,350,675 + Pf6N2E2_3734 0.48 +2.0 4,350,675 + Pf6N2E2_3734 0.48 +0.7 4,350,675 + Pf6N2E2_3734 0.48 +0.5 4,350,675 + Pf6N2E2_3734 0.48 +1.1 4,350,675 + Pf6N2E2_3734 0.48 +0.6 4,350,675 + Pf6N2E2_3734 0.48 +1.6 4,350,675 + Pf6N2E2_3734 0.48 +0.0 4,350,675 + Pf6N2E2_3734 0.48 +1.1 4,350,675 + Pf6N2E2_3734 0.48 +0.8 4,350,676 - Pf6N2E2_3734 0.48 +0.3 4,350,676 - Pf6N2E2_3734 0.48 -0.2 4,350,676 - Pf6N2E2_3734 0.48 +0.3 4,350,676 - Pf6N2E2_3734 0.48 +1.4 4,350,676 - Pf6N2E2_3734 0.48 +0.1 4,350,676 - Pf6N2E2_3734 0.48 +0.4 4,350,676 - Pf6N2E2_3734 0.48 +0.4 4,350,676 - Pf6N2E2_3734 0.48 +0.9 4,350,676 - Pf6N2E2_3734 0.48 -0.2 4,350,676 - Pf6N2E2_3734 0.48 +0.4 4,350,676 - Pf6N2E2_3734 0.48 +0.3 4,350,676 - Pf6N2E2_3734 0.48 +0.7 4,350,676 - Pf6N2E2_3734 0.48 -0.1 4,350,682 - Pf6N2E2_3734 0.49 -0.6 4,350,704 + Pf6N2E2_3734 0.52 +2.0 4,350,704 + Pf6N2E2_3734 0.52 -0.7 4,350,705 - Pf6N2E2_3734 0.52 +1.7 4,350,802 - Pf6N2E2_3734 0.66 +0.3 4,350,912 + Pf6N2E2_3734 0.82 +0.4 4,350,912 + Pf6N2E2_3734 0.82 +1.4 4,350,912 + Pf6N2E2_3734 0.82 +0.7 4,350,912 + Pf6N2E2_3734 0.82 +1.2 4,350,912 + Pf6N2E2_3734 0.82 +0.4 4,350,912 + Pf6N2E2_3734 0.82 +1.9 4,350,912 + Pf6N2E2_3734 0.82 +0.4 4,350,912 + Pf6N2E2_3734 0.82 -0.1 4,350,913 - Pf6N2E2_3734 0.82 +0.4 4,350,913 - Pf6N2E2_3734 0.82 +0.2 4,350,913 - Pf6N2E2_3734 0.82 +3.0 4,350,913 - Pf6N2E2_3734 0.82 -0.4 4,350,913 - Pf6N2E2_3734 0.82 -0.1 4,350,913 - Pf6N2E2_3734 0.82 +0.4 4,350,984 + -1.2 4,350,985 - +2.7 4,351,036 + +0.0 4,351,036 + +0.6 4,351,038 + +0.4 4,351,039 - +0.9 4,351,039 - +0.1 4,351,066 + -0.6 4,351,076 + -0.5 4,351,092 + -0.8 4,351,092 + +1.0 4,351,092 + +1.0 4,351,092 + +0.4 4,351,093 - +0.7 4,351,093 - +0.9 4,351,093 - +0.4 4,351,093 - +0.3 4,351,093 - +1.4 4,351,093 - +0.5 4,351,113 - +1.0 4,351,116 + +1.9 4,351,116 + +0.8 4,351,116 + +2.0 4,351,116 + +0.0 4,351,116 + +0.1 4,351,116 + -2.2 4,351,116 + +0.2 4,351,116 + +1.4 4,351,116 + +0.4 4,351,116 + +0.7 4,351,116 + +2.7 4,351,117 - -0.1 4,351,117 - +0.0 4,351,117 - +0.3 4,351,117 - +0.8 4,351,117 - -0.4 4,351,117 - +0.0 4,351,117 - +0.1 4,351,117 - +0.2 4,351,117 - -1.6 4,351,117 - -0.3 4,351,117 - +0.1 4,351,117 - +0.4 4,351,117 - -0.6 4,351,117 - +0.4 4,351,154 + +0.0 4,351,159 + -2.3 4,351,159 + +0.8 4,351,159 + +0.4 4,351,159 + +0.1 4,351,160 - +0.6 4,351,160 - -0.6 4,351,388 + +0.5 4,351,388 + +1.0 4,351,388 + +0.4 4,351,388 + +0.8 4,351,388 + +1.1 4,351,388 + -0.3 4,351,388 + +0.0 4,351,388 + -0.9 4,351,389 - +0.1 4,351,389 - -0.1 4,351,389 - +1.0 4,351,389 - +0.0 4,351,389 - +0.4 4,351,390 + -0.2 4,351,390 + +0.1 4,351,390 + -0.5 4,351,391 - -0.3 4,351,391 - +0.7 4,351,391 - -0.2 4,351,391 - +0.1 4,351,391 - +0.4 4,351,391 - +1.1 4,351,391 - +0.4 4,351,404 - -0.0 4,351,431 - -0.6 4,351,431 - -0.1 4,351,432 + -0.9 4,351,569 + Pf6N2E2_3735 0.18 +0.3 4,351,570 - Pf6N2E2_3735 0.18 +1.4 4,351,570 - Pf6N2E2_3735 0.18 -0.7 4,351,714 - Pf6N2E2_3735 0.34 +0.0 4,351,892 + Pf6N2E2_3735 0.53 -0.8
Or see this region's nucleotide sequence